##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII Acc65I AclI AgeI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AspI BbrPI BcgI BsaAI BsiWI
BspDI BspLU11I BssHII Bst1107I BstAPI
BstBI BstZ17I ClaI CpoI DraIII
Eco47III EcoRV FseI HpaI KpnI
KspI MfeI MunI NaeI NgoMIV
NheI NotI NruI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SbfI SexAI SfiI SfuI SgrAI
SnaBI Sse8387I SspI StuI SwaI
Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccII 390 CGCG CG^CG
AccIII 2011 TCCGGA T^CCGGA
AflII 2277 CTTAAG C^TTAAG
AflIII 389 ACGCGT A^C[GA][CT]GT
AhdI 1021 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 4201 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1214 CCTCAGG CC^T[ACGT]AGG
AspEI 1021 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1321 TGCGCA TGC^GCA
AvrII 870 CCTAGG C^CTAGG
BamHI 2174 GGATCC G^GATCC
BbeI 364 GGCGCC GGCGC^C
BciVI 1489 GTATCC GTATCC(6/5)
BfrI 2277 CTTAAG C^TTAAG
BglII 506 AGATCT A^GATCT
BlnI 870 CCTAGG C^CTAGG
BsaBI 918 GATGGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2011 TCCGGA T^CCGGA
BsmBI 2655 GAGACG CGTCTC(1/5)
BspEI 2011 TCCGGA T^CCGGA
BspHI 4150 TCATGA T^CATGA
BsrFI 463 GCCGGT [GA]^CCGG[CT]
BssSI 1651 CTCGTG CACGAG(-5/-1)
BstEII 962 GGTGACC G^GT[ACGT]ACC
BstPI 962 GGTGACC G^GT[ACGT]ACC
BstUI 390 CGCG CG^CG
BstXI 292 CCAGCATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1214 CCTCAGG CC^T[ACGT]AGG
Cfr10I 463 GCCGGT [GA]^CCGG[CT]
DrdI 4382 GACTCCTTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1021 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1214 CCTCAGG CC^T[ACGT]AGG
EcoO65I 962 GGTGACC G^GT[ACGT]ACC
EcoRI 4578 GAATTC G^AATTC
EcoT22I 2827 ATGCAT ATGCA^T
FspI 1321 TGCGCA TGC^GCA
KasI 364 GGCGCC G^GCGCC
MamI 918 GATGGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 389 ACGCGT A^CGCGT
MroI 2011 TCCGGA T^CCGGA
MslI 2850 CATTCATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1420 CAGCTG C[AC]G^C[GT]G
MvnI 390 CGCG CG^CG
NarI 364 GGCGCC GG^CGCC
NsiI 2827 ATGCAT ATGCA^T
PaeR7I 2221 CTCGAG C^TCGAG
PshAI 677 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1420 CAGCTG CAG^CTG
RcaI 4150 TCATGA T^CATGA
SalI 284 GTCGAC G^TCGAC
SfoI 364 GGCGCC GGC^GCC
SpeI 2211 ACTAGT A^CTAGT
SphI 2510 GCATGC GCATG^C
XhoI 2221 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 284 GTCGAC GT^[AC][GT]AC
1018 GTAGAC
Alw44I 1533 GTGCAC G^TGCAC
4638 GTGCAC
ApaLI 1533 GTGCAC G^TGCAC
4638 GTGCAC
BalI 427 TGGCCA TGG^CCA
3997 TGGCCA
BclI 1256 TGATCA T^GATCA
2683 TGATCA
BlpI 1281 GCTCAGC GC^T[ACGT]AGC
2409 GCTGAGC
Bpu1102I 1281 GCTCAGC GC^T[ACGT]AGC
2409 GCTGAGC
BsaWI 2011 TCCGGA [AT]^CCGG[AT]
2884 ACCGGA
BsgI 4780 CTGCAC GTGCAG(16/14)
4307 GTGCAG
CelII 1281 GCTCAGC GC^T[ACGT]AGC
2409 GCTGAGC
EagI 461 CGGCCG C^GGCCG
2314 CGGCCG
EclXI 461 CGGCCG C^GGCCG
2314 CGGCCG
Eco52I 461 CGGCCG C^GGCCG
2314 CGGCCG
FauI 852 GCGGG CCCGC(4/6)
1292 GCGGG
FbaI 1256 TGATCA T^GATCA
2683 TGATCA
MluNI 427 TGGCCA TGG^CCA
3997 TGGCCA
MscI 427 TGGCCA TGG^CCA
3997 TGGCCA
NspI 1745 ACATGC [GA]CATG^[CT]
2510 GCATGC
PflMI 788 CCAGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1773 CCACATTCTGG
SapI 2376 GCTCTTC GCTCTTC(1/4)
4847 GCTCTTC
SmaI 343 CCCGGG CCC^GGG
958 CCCGGG
Van91I 788 CCAGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1773 CCACATTCTGG
XbaI 113 TCTAGA T^CTAGA
2832 TCTAGA
XmaCI 343 CCCGGG C^CCGGG
958 CCCGGG
XmaI 343 CCCGGG C^CCGGG
958 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApaI ApoI AspHI AvaI
AvaII BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspMI BsrBI BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspI
MvaI MwoI NciI NcoI NdeI
NdeII NlaIII NlaIV PleI PpuMI
PstI RsaI SacI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII