##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI AocI AscI AseI AsnI
Asp718I AspEI AviII BamHI BclI
BfrI BsaBI BsaI BseAI BsiWI
Bsp1407I BspDI BspEI BspLU11I BsrGI
Bst1107I BstBI BstZ17I Bsu36I ClaI
CpoI DrdI Eam1105I Eco81I EcoT22I
FbaI FspI HincII HindII HpaI
KpnI KspI MamI MfeI MluI
MroI MunI NheI NotI NsiI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I RsrII SacII SalI
SapI SbfI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 2272 GACGTC GACGT^C
AflIII 2053 ACACGT A^C[GA][CT]GT
Aor51HI 542 AGCGCT AGC^GCT
ApaI 95 GGGCCC GGGCC^C
Asp700I 3866 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 4324 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 340 CCTAGG C^CTAGG
BbeI 3247 GGCGCC GGCGC^C
BbrPI 2918 CACGTG CAC^GTG
BbsI 739 GAAGAC GAAGAC(2/6)
BbvCI 1282 CCTCAGC CCTCAGC(-5/-2)
BcgI 366 CGACGACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 583 GTATCC GTATCC(6/5)
BglII 2538 AGATCT A^GATCT
BlnI 340 CCTAGG C^CTAGG
BpuAI 739 GAAGAC GAAGAC(2/6)
BsaWI 1590 TCCGGT [AT]^CCGG[AT]
BsmBI 4015 CGTCTC CGTCTC(1/5)
BsmI 1971 GCATTC GAATGC(1/-1)
BspHI 2437 TCATGA T^CATGA
BssHII 123 GCGCGC G^CGCGC
DraIII 1083 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 542 AGCGCT AGC^GCT
EcoRI 674 GAATTC G^AATTC
EcoRV 4141 GATATC GAT^ATC
FseI 318 GGCCGGCC GGCCGG^CC
KasI 3247 GGCGCC G^GCGCC
NarI 3247 GGCGCC GG^CGCC
NruI 563 TCGCGA TCG^CGA
PflFI 4324 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2918 CACGTG CAC^GTG
PmlI 2918 CACGTG CAC^GTG
PvuI 1805 CGATCG CGAT^CG
RcaI 2437 TCATGA T^CATGA
ScaI 3623 AGTACT AGT^ACT
SexAI 1320 ACCTGGT A^CC[AT]GGT
SfoI 3247 GGCGCC GGC^GCC
SphI 1981 GCATGC GCATG^C
SspI 794 AATATT AAT^ATT
SwaI 631 ATTTAAAT ATTT^AAAT
TaqI 2 TCGA T^CGA
Tth111I 4324 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 2 TCGA T^CGA
XbaI 4472 TCTAGA T^CTAGA
XmnI 3866 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 2057 GTCTAC GT^[AC][GT]AC
3222 GTAGAC
Alw44I 3269 GTGCAC G^TGCAC
3790 GTGCAC
ApaLI 3269 GTGCAC G^TGCAC
3790 GTGCAC
BsaAI 2496 TACGTG [CT]AC^GT[GA]
2918 CACGTG
BspMI 2646 ACCTGC ACCTGC(4/8)
916 GCAGGT
BstEII 919 GGTCACC G^GT[ACGT]ACC
4066 GGTGACC
BstPI 919 GGTCACC G^GT[ACGT]ACC
4066 GGTGACC
BstXI 1033 CCAGCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2404 CCACACAGATGG
EcoNI 654 CCTGTGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2992 CCTCCAGAAGG
EcoO65I 919 GGTCACC G^GT[ACGT]ACC
4066 GGTGACC
HindIII 2171 AAGCTT A^AGCTT
4610 AAGCTT
NaeI 319 GCCGGC GCC^GGC
329 GCCGGC
NcoI 2008 CCATGG C^CATGG
4259 CCATGG
NdeI 3876 CATATG CA^TATG
4271 CATATG
NgoMIV 319 GCCGGC G^CCGGC
329 GCCGGC
NspI 1981 GCATGC [GA]CATG^[CT]
4455 GCATGT
PshAI 1487 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2380 GACCCTGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaHI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrBI BsrDI BsrFI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EagI EarI EclXI
Eco52I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XcmI XhoII XmaCI
XmaI