################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## Acc65I AclI AgeI AocI AscI AseI AsnI Asp718I AspI AviII AvrII BbvCI BcgI BclI BlnI BsiEI BsiWI BspDI Bst1107I BstZ17I Bsu36I ClaI CpoI EagI EclXI Eco52I Eco81I EcoRV EcoT22I FbaI FseI FspI KpnI KspI MfeI MluI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SgrAI SnaBI SpeI Sse8387I SspI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AatII 2009 GACGTC GACGT^C AflII 4727 CTTAAG C^TTAAG Alw44I 5099 GTGCAC G^TGCAC Aor51HI 3461 AGCGCT AGC^GCT ApaI 5853 GGGCCC GGGCC^C ApaLI 5099 GTGCAC G^TGCAC AvaI 523 CCCGGG C^[CT]CG[GA]G BalI 32 TGGCCA TGG^CCA BbeI 4668 GGCGCC GGCGC^C BciVI 4054 GTATCC GTATCC(6/5) BfrI 4727 CTTAAG C^TTAAG BglI 3959 GCCTTCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 296 GCTAAGC GC^T[ACGT]AGC Bpu1102I 296 GCTAAGC GC^T[ACGT]AGC BsmBI 3767 GAGACG CGTCTC(1/5) BsoBI 523 CCCGGG C^[CT]CG[GA]G Bsp1407I 1125 TGTACA T^GTACA BspHI 5372 TCATGA T^CATGA BsrBI 1150 CCGCTC CCGCTC(-3/-3) BsrFI 4713 ACCGGC [GA]^CCGG[CT] BsrGI 1125 TGTACA T^GTACA BssHII 2694 GCGCGC G^CGCGC BssSI 3349 CACGAG CACGAG(-5/-1) BstAPI 2301 GCATGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 3831 TTCGAA TT^CGAA BstEII 1840 GGTGACC G^GT[ACGT]ACC BstPI 1840 GGTGACC G^GT[ACGT]ACC CelII 296 GCTAAGC GC^T[ACGT]AGC Cfr10I 4713 ACCGGC [GA]^CCGG[CT] Eco47III 3461 AGCGCT AGC^GCT EcoO65I 1840 GGTGACC G^GT[ACGT]ACC HindIII 3118 AAGCTT A^AGCTT HpaI 6 GTTAAC GTT^AAC KasI 4668 GGCGCC G^GCGCC MluNI 32 TGGCCA TGG^CCA MscI 32 TGGCCA TGG^CCA NarI 4668 GGCGCC GG^CGCC NspV 3831 TTCGAA TT^CGAA PshAI 2725 GACTTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 5372 TCATGA T^CATGA SfoI 4668 GGCGCC GGC^GCC SfuI 3831 TTCGAA TT^CGAA SmaI 523 CCCGGG CCC^GGG SphI 2301 GCATGC GCATG^C SspBI 1125 TGTACA T^GTACA SwaI 4456 ATTTAAAT ATTT^AAAT XbaI 3934 TCTAGA T^CTAGA XmaCI 523 CCCGGG C^CCGGG XmaI 523 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccIII 1743 TCCGGA T^CCGGA 3591 TCCGGA AhdI 344 GACGAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 587 GACCGCGAGTC AspEI 344 GACGAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 587 GACCGCGAGTC BbsI 4699 GAAGAC GAAGAC(2/6) 2844 GTCTTC BglII 2041 AGATCT A^GATCT 4176 AGATCT BpuAI 4699 GAAGAC GAAGAC(2/6) 2844 GTCTTC BsaBI 3046 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3578 GATGCAGATC BseAI 1743 TCCGGA T^CCGGA 3591 TCCGGA BspEI 1743 TCCGGA T^CCGGA 3591 TCCGGA BspLU11I 473 ACATGT A^CATGT 5998 ACATGT BsrDI 2257 GCAATG GCAATG(2/0) 3363 GCAATG DraI 4457 TTTAAA TTT^AAA 5909 TTTAAA EaeI 32 TGGCCA [CT]^GGCC[GA] 4574 CGGCCA Eam1105I 344 GACGAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 587 GACCGCGAGTC HincII 6 GTTAAC GT[CT]^[GA]AC 584 GTTGAC HindII 6 GTTAAC GT[CT]^[GA]AC 584 GTTGAC MamI 3046 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3578 GATGCAGATC MroI 1743 TCCGGA T^CCGGA 3591 TCCGGA ScaI 1443 AGTACT AGT^ACT 5558 AGTACT StuI 3554 AGGCCT AGG^CCT 3957 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 124 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AvaII BamHI BanI BanII BbrPI BbvI BcnI BfaI BmyI BpmI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DrdI DsaI EarI EcoNI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI