##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
Acc65I AclI AgeI AocI AscI
AseI AsnI Asp718I AspI AviII
AvrII BbvCI BcgI BclI BlnI
BsiEI BsiWI BspDI Bst1107I BstZ17I
Bsu36I ClaI CpoI EagI EclXI
Eco52I Eco81I EcoRV EcoT22I FbaI
FseI FspI KpnI KspI MfeI
MluI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmeI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfiI SgrAI SnaBI
SpeI Sse8387I SspI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 2009 GACGTC GACGT^C
AflII 4727 CTTAAG C^TTAAG
Alw44I 5099 GTGCAC G^TGCAC
Aor51HI 3461 AGCGCT AGC^GCT
ApaI 5853 GGGCCC GGGCC^C
ApaLI 5099 GTGCAC G^TGCAC
AvaI 523 CCCGGG C^[CT]CG[GA]G
BalI 32 TGGCCA TGG^CCA
BbeI 4668 GGCGCC GGCGC^C
BciVI 4054 GTATCC GTATCC(6/5)
BfrI 4727 CTTAAG C^TTAAG
BglI 3959 GCCTTCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 296 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 296 GCTAAGC GC^T[ACGT]AGC
BsmBI 3767 GAGACG CGTCTC(1/5)
BsoBI 523 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1125 TGTACA T^GTACA
BspHI 5372 TCATGA T^CATGA
BsrBI 1150 CCGCTC CCGCTC(-3/-3)
BsrFI 4713 ACCGGC [GA]^CCGG[CT]
BsrGI 1125 TGTACA T^GTACA
BssHII 2694 GCGCGC G^CGCGC
BssSI 3349 CACGAG CACGAG(-5/-1)
BstAPI 2301 GCATGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3831 TTCGAA TT^CGAA
BstEII 1840 GGTGACC G^GT[ACGT]ACC
BstPI 1840 GGTGACC G^GT[ACGT]ACC
CelII 296 GCTAAGC GC^T[ACGT]AGC
Cfr10I 4713 ACCGGC [GA]^CCGG[CT]
Eco47III 3461 AGCGCT AGC^GCT
EcoO65I 1840 GGTGACC G^GT[ACGT]ACC
HindIII 3118 AAGCTT A^AGCTT
HpaI 6 GTTAAC GTT^AAC
KasI 4668 GGCGCC G^GCGCC
MluNI 32 TGGCCA TGG^CCA
MscI 32 TGGCCA TGG^CCA
NarI 4668 GGCGCC GG^CGCC
NspV 3831 TTCGAA TT^CGAA
PshAI 2725 GACTTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 5372 TCATGA T^CATGA
SfoI 4668 GGCGCC GGC^GCC
SfuI 3831 TTCGAA TT^CGAA
SmaI 523 CCCGGG CCC^GGG
SphI 2301 GCATGC GCATG^C
SspBI 1125 TGTACA T^GTACA
SwaI 4456 ATTTAAAT ATTT^AAAT
XbaI 3934 TCTAGA T^CTAGA
XmaCI 523 CCCGGG C^CCGGG
XmaI 523 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccIII 1743 TCCGGA T^CCGGA
3591 TCCGGA
AhdI 344 GACGAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
587 GACCGCGAGTC
AspEI 344 GACGAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
587 GACCGCGAGTC
BbsI 4699 GAAGAC GAAGAC(2/6)
2844 GTCTTC
BglII 2041 AGATCT A^GATCT
4176 AGATCT
BpuAI 4699 GAAGAC GAAGAC(2/6)
2844 GTCTTC
BsaBI 3046 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3578 GATGCAGATC
BseAI 1743 TCCGGA T^CCGGA
3591 TCCGGA
BspEI 1743 TCCGGA T^CCGGA
3591 TCCGGA
BspLU11I 473 ACATGT A^CATGT
5998 ACATGT
BsrDI 2257 GCAATG GCAATG(2/0)
3363 GCAATG
DraI 4457 TTTAAA TTT^AAA
5909 TTTAAA
EaeI 32 TGGCCA [CT]^GGCC[GA]
4574 CGGCCA
Eam1105I 344 GACGAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
587 GACCGCGAGTC
HincII 6 GTTAAC GT[CT]^[GA]AC
584 GTTGAC
HindII 6 GTTAAC GT[CT]^[GA]AC
584 GTTGAC
MamI 3046 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3578 GATGCAGATC
MroI 1743 TCCGGA T^CCGGA
3591 TCCGGA
ScaI 1443 AGTACT AGT^ACT
5558 AGTACT
StuI 3554 AGGCCT AGG^CCT
3957 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApoI Asp700I AspHI AvaII BamHI
BanI BanII BbrPI BbvI BcnI
BfaI BmyI BpmI BsaAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DraIII DrdI DsaI EarI
EcoNI EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmnI