##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AccI AccIII AclI AflII AgeI
AocI AscI AseI AsnI AvrII
BamHI BbeI BcgI BfrI BlnI
BseAI BsiWI BspDI BspEI BspHI
BsrDI Bst1107I BstEII BstPI BstZ17I
Bsu36I ClaI DraI DraIII EagI
EclXI Eco52I Eco81I EcoO65I EcoT22I
FseI HpaI KasI KspI MfeI
MroI MunI NarI NdeI NotI
NruI NsiI PacI PflMI PinAI
PmeI PshBI Psp1406I PvuI RcaI
SacII SalI SbfI SfiI SfoI
SgrAI SnaBI Sse8387I StuI SwaI
Van91I
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AatII 2473 GACGTC GACGT^C
Acc65I 5391 GGTACC G^GTACC
Asp700I 1414 GAATGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 5391 GGTACC G^GTACC
AviII 2097 TGCGCA TGC^GCA
BglII 3012 AGATCT A^GATCT
BsaBI 5193 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 72 CGGTCG CG[GA][CT]^CG
Bsp1407I 2964 TGTACA T^GTACA
BsrGI 2964 TGTACA T^GTACA
BstBI 417 TTCGAA TT^CGAA
DrdI 787 GACATCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 6015 GAATTC G^AATTC
FspI 2097 TGCGCA TGC^GCA
HincII 2249 GTCAAC GT[CT]^[GA]AC
HindII 2249 GTCAAC GT[CT]^[GA]AC
HindIII 1809 AAGCTT A^AGCTT
KpnI 5391 GGTACC GGTAC^C
MamI 5193 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 3929 ACGCGT A^CGCGT
NaeI 67 GCCGGC GCC^GGC
NgoMIV 67 GCCGGC G^CCGGC
NheI 1714 GCTAGC G^CTAGC
NspV 417 TTCGAA TT^CGAA
PshAI 3834 GACCGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 1869 ACCAGGT A^CC[AT]GGT
SfuI 417 TTCGAA TT^CGAA
SmaI 2700 CCCGGG CCC^GGG
SpeI 6135 ACTAGT A^CTAGT
SphI 4627 GCATGC GCATG^C
SspBI 2964 TGTACA T^GTACA
SspI 6634 AATATT AAT^ATT
XmaCI 2700 CCCGGG C^CCGGG
XmaI 2700 CCCGGG C^CCGGG
XmnI 1414 GAATGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 640 AAATTC [GA]^AATT[CT]
6015 GAATTC
AcyI 836 GACGCC G[GA]^CG[CT]C
2473 GACGTC
AhdI 866 GACACCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2537 GACCGGAAGTC
AlwI 88 GGATC GGATC(4/5)
2488 GGATC
Aor51HI 2481 AGCGCT AGC^GCT
4514 AGCGCT
ApoI 640 AAATTC [GA]^AATT[CT]
6015 GAATTC
AspEI 866 GACACCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2537 GACCGGAAGTC
AspI 4353 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
5903 GACAGGGTC
BbrPI 222 CACGTG CAC^GTG
3444 CACGTG
BciVI 4442 GTATCC GTATCC(6/5)
6332 GTATCC
BsaHI 836 GACGCC G[GA]^CG[CT]C
2473 GACGTC
BsmBI 873 CGTCTC CGTCTC(1/5)
2475 CGTCTC
BspLU11I 1699 ACATGT A^CATGT
5227 ACATGT
BssHII 32 GCGCGC G^CGCGC
34 GCGCGC
BssSI 698 CACGAG CACGAG(-5/-1)
6235 CACGAG
CpoI 58 CGGTCCG CG^G[AT]CCG
2970 CGGACCG
Eam1105I 866 GACACCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2537 GACCGGAAGTC
Eco47III 2481 AGCGCT AGC^GCT
4514 AGCGCT
EcoNI 1192 CCTTTTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1339 CCTACGGAAGG
EcoRV 2777 GATATC GAT^ATC
4106 GATATC
Hin1I 836 GACGCC G[GA]^CG[CT]C
2473 GACGTC
NcoI 5742 CCATGG C^CATGG
6024 CCATGG
PflFI 4353 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
5903 GACAGGGTC
PmaCI 222 CACGTG CAC^GTG
3444 CACGTG
PmlI 222 CACGTG CAC^GTG
3444 CACGTG
RsrII 58 CGGTCCG CG^G[AT]CCG
2970 CGGACCG
Tth111I 4353 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
5903 GACAGGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
Alw44I AlwNI ApaI ApaLI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BclI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PaeR7I PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI XcmI XhoI XhoII