##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
Aor51HI AscI Asp718I AviII BbeI
BcgI BciVI BclI BglII BsaHI
BsaWI BseAI BsiWI BspDI BspEI
BsrBI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI Eco47III EcoRV EcoT22I
FbaI FseI FspI HaeII Hin1I
KasI KpnI KspI MluI MroI
NarI NheI NotI NruI NsiI
NspV PacI PinAI PmeI PshAI
PvuI RsrII SacII SalI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AclI 5771 AACGTT AA^CGTT
AflII 3590 CTTAAG C^TTAAG
AseI 754 ATTAAT AT^TAAT
AsnI 754 ATTAAT AT^TAAT
Asp700I 596 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 4462 CTCGAG C^[CT]CG[GA]G
BbrPI 4260 CACGTG CAC^GTG
BbsI 2602 GTCTTC GAAGAC(2/6)
BbvCI 5542 GCTGAGG CCTCAGC(-5/-2)
BfrI 3590 CTTAAG C^TTAAG
BpuAI 2602 GTCTTC GAAGAC(2/6)
BsaAI 4260 CACGTG [CT]AC^GT[GA]
BsiEI 1334 CGGCCG CG[GA][CT]^CG
BsmI 2294 GCATTC GAATGC(1/-1)
BsoBI 4462 CTCGAG C^[CT]CG[GA]G
Bsp1407I 3551 TGTACA T^GTACA
BsrGI 3551 TGTACA T^GTACA
DrdI 2593 GACACTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1334 CGGCCG C^GGCCG
EclXI 1334 CGGCCG C^GGCCG
Eco52I 1334 CGGCCG C^GGCCG
HgaI 529 GACGC GACGC(5/10)
MfeI 146 CAATTG C^AATTG
MunI 146 CAATTG C^AATTG
NaeI 5899 GCCGGC GCC^GGC
NcoI 5815 CCATGG C^CATGG
NdeI 3228 CATATG CA^TATG
NgoMIV 5899 GCCGGC G^CCGGC
PaeR7I 4462 CTCGAG C^TCGAG
PmaCI 4260 CACGTG CAC^GTG
PmlI 4260 CACGTG CAC^GTG
PshBI 754 ATTAAT AT^TAAT
Psp1406I 5771 AACGTT AA^CGTT
SacI 3610 GAGCTC GAGCT^C
SbfI 3288 CCTGCAGG CCTGCA^GG
ScaI 3973 AGTACT AGT^ACT
Sse8387I 3288 CCTGCAGG CCTGCA^GG
SspBI 3551 TGTACA T^GTACA
XbaI 643 TCTAGA T^CTAGA
XhoI 4462 CTCGAG C^TCGAG
XmnI 596 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 3846 GTCTAC GT^[AC][GT]AC
3930 GTAGAC
AccII 12 CGCG CG^CG
1633 CGCG
AhdI 3829 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4432 GACCCTATGTC
ApaI 1853 GGGCCC GGGCC^C
2538 GGGCCC
AspEI 3829 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4432 GACCCTATGTC
AspI 3332 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3933 GACTCTGTC
BamHI 3490 GGATCC G^GATCC
4653 GGATCC
BcnI 547 CCGGG CC^[GC]GG
2973 CCCGG
BsaBI 2481 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2903 GATGATAATC
BsaI 988 GAGACC GGTCTC(1/5)
1399 GGTCTC
BsmBI 209 CGTCTC CGTCTC(1/5)
527 GAGACG
BspHI 1655 TCATGA T^CATGA
5374 TCATGA
BsrFI 4846 GCCGGT [GA]^CCGG[CT]
5899 GCCGGC
BssSI 1547 CACGAG CACGAG(-5/-1)
2807 CTCGTG
BstAPI 4044 GCAATTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5851 GCAGCTGCTGC
BstUI 12 CGCG CG^CG
1633 CGCG
Cfr10I 4846 GCCGGT [GA]^CCGG[CT]
5899 GCCGGC
DraI 5003 TTTAAA TTT^AAA
5626 TTTAAA
DraIII 4155 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
5357 CACTTGGTG
Eam1105I 3829 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4432 GACCCTATGTC
EcoRI 2801 GAATTC G^AATTC
5042 GAATTC
HindIII 1538 AAGCTT A^AGCTT
5490 AAGCTT
HpaI 2654 GTTAAC GTT^AAC
5032 GTTAAC
MamI 2481 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2903 GATGATAATC
MvnI 12 CGCG CG^CG
1633 CGCG
NciI 547 CCGGG CC^[GC]GG
2973 CCCGG
PflFI 3332 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3933 GACTCTGTC
RcaI 1655 TCATGA T^CATGA
5374 TCATGA
SphI 3576 GCATGC GCATG^C
3920 GCATGC
SspI 4949 AATATT AAT^ATT
4957 AATATT
Tth111I 3332 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3933 GACTCTGTC
XcmI 2874 CCACCAGGAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4533 CCACAGGCCTGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
Alw44I AlwI AlwNI AocI ApaLI
ApoI AspHI AvaII AvrII BalI
BanI BanII BbvI BfaI BglI
BlnI BlpI BmyI BpmI Bpu1102I
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspLU11I
BspMI BsrDI BsrI BssKI BstEII
BstF5I BstNI BstPI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII