################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## AatII Acc65I AccIII AcyI AgeI Aor51HI AscI Asp718I AviII BbeI BcgI BciVI BclI BglII BsaHI BsaWI BseAI BsiWI BspDI BspEI BsrBI BssHII Bst1107I BstBI BstZ17I ClaI CpoI Eco47III EcoRV EcoT22I FbaI FseI FspI HaeII Hin1I KasI KpnI KspI MluI MroI NarI NheI NotI NruI NsiI NspV PacI PinAI PmeI PshAI PvuI RsrII SacII SalI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AclI 5771 AACGTT AA^CGTT AflII 3590 CTTAAG C^TTAAG AseI 754 ATTAAT AT^TAAT AsnI 754 ATTAAT AT^TAAT Asp700I 596 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 4462 CTCGAG C^[CT]CG[GA]G BbrPI 4260 CACGTG CAC^GTG BbsI 2602 GTCTTC GAAGAC(2/6) BbvCI 5542 GCTGAGG CCTCAGC(-5/-2) BfrI 3590 CTTAAG C^TTAAG BpuAI 2602 GTCTTC GAAGAC(2/6) BsaAI 4260 CACGTG [CT]AC^GT[GA] BsiEI 1334 CGGCCG CG[GA][CT]^CG BsmI 2294 GCATTC GAATGC(1/-1) BsoBI 4462 CTCGAG C^[CT]CG[GA]G Bsp1407I 3551 TGTACA T^GTACA BsrGI 3551 TGTACA T^GTACA DrdI 2593 GACACTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1334 CGGCCG C^GGCCG EclXI 1334 CGGCCG C^GGCCG Eco52I 1334 CGGCCG C^GGCCG HgaI 529 GACGC GACGC(5/10) MfeI 146 CAATTG C^AATTG MunI 146 CAATTG C^AATTG NaeI 5899 GCCGGC GCC^GGC NcoI 5815 CCATGG C^CATGG NdeI 3228 CATATG CA^TATG NgoMIV 5899 GCCGGC G^CCGGC PaeR7I 4462 CTCGAG C^TCGAG PmaCI 4260 CACGTG CAC^GTG PmlI 4260 CACGTG CAC^GTG PshBI 754 ATTAAT AT^TAAT Psp1406I 5771 AACGTT AA^CGTT SacI 3610 GAGCTC GAGCT^C SbfI 3288 CCTGCAGG CCTGCA^GG ScaI 3973 AGTACT AGT^ACT Sse8387I 3288 CCTGCAGG CCTGCA^GG SspBI 3551 TGTACA T^GTACA XbaI 643 TCTAGA T^CTAGA XhoI 4462 CTCGAG C^TCGAG XmnI 596 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccI 3846 GTCTAC GT^[AC][GT]AC 3930 GTAGAC AccII 12 CGCG CG^CG 1633 CGCG AhdI 3829 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4432 GACCCTATGTC ApaI 1853 GGGCCC GGGCC^C 2538 GGGCCC AspEI 3829 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4432 GACCCTATGTC AspI 3332 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3933 GACTCTGTC BamHI 3490 GGATCC G^GATCC 4653 GGATCC BcnI 547 CCGGG CC^[GC]GG 2973 CCCGG BsaBI 2481 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2903 GATGATAATC BsaI 988 GAGACC GGTCTC(1/5) 1399 GGTCTC BsmBI 209 CGTCTC CGTCTC(1/5) 527 GAGACG BspHI 1655 TCATGA T^CATGA 5374 TCATGA BsrFI 4846 GCCGGT [GA]^CCGG[CT] 5899 GCCGGC BssSI 1547 CACGAG CACGAG(-5/-1) 2807 CTCGTG BstAPI 4044 GCAATTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 5851 GCAGCTGCTGC BstUI 12 CGCG CG^CG 1633 CGCG Cfr10I 4846 GCCGGT [GA]^CCGG[CT] 5899 GCCGGC DraI 5003 TTTAAA TTT^AAA 5626 TTTAAA DraIII 4155 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG 5357 CACTTGGTG Eam1105I 3829 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4432 GACCCTATGTC EcoRI 2801 GAATTC G^AATTC 5042 GAATTC HindIII 1538 AAGCTT A^AGCTT 5490 AAGCTT HpaI 2654 GTTAAC GTT^AAC 5032 GTTAAC MamI 2481 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2903 GATGATAATC MvnI 12 CGCG CG^CG 1633 CGCG NciI 547 CCGGG CC^[GC]GG 2973 CCCGG PflFI 3332 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3933 GACTCTGTC RcaI 1655 TCATGA T^CATGA 5374 TCATGA SphI 3576 GCATGC GCATG^C 3920 GCATGC SspI 4949 AATATT AAT^ATT 4957 AATATT Tth111I 3332 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3933 GACTCTGTC XcmI 2874 CCACCAGGAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4533 CCACAGGCCTGTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 120 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI Alw44I AlwI AlwNI AocI ApaLI ApoI AspHI AvaII AvrII BalI BanI BanII BbvI BfaI BglI BlnI BlpI BmyI BpmI Bpu1102I BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspLU11I BspMI BsrDI BsrI BssKI BstEII BstF5I BstNI BstPI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII