##################################
# NO CUT ENZYMES (   65 enzymes) #
##################################

AatII       Acc65I      AccIII      AcyI        AgeI        
Aor51HI     AscI        Asp718I     AviII       BbeI        
BcgI        BciVI       BclI        BglII       BsaHI       
BsaWI       BseAI       BsiWI       BspDI       BspEI       
BsrBI       BssHII      Bst1107I    BstBI       BstZ17I     
ClaI        CpoI        Eco47III    EcoRV       EcoT22I     
FbaI        FseI        FspI        HaeII       Hin1I       
KasI        KpnI        KspI        MluI        MroI        
NarI        NheI        NotI        NruI        NsiI        
NspV        PacI        PinAI       PmeI        PshAI       
PvuI        RsrII       SacII       SalI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SwaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AclI         5771  AACGTT               AA^CGTT
AflII        3590  CTTAAG               C^TTAAG
AseI          754  ATTAAT               AT^TAAT
AsnI          754  ATTAAT               AT^TAAT
Asp700I       596  GAAGCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI         4462  CTCGAG               C^[CT]CG[GA]G
BbrPI        4260  CACGTG               CAC^GTG
BbsI         2602  GTCTTC               GAAGAC(2/6)
BbvCI        5542  GCTGAGG              CCTCAGC(-5/-2)
BfrI         3590  CTTAAG               C^TTAAG
BpuAI        2602  GTCTTC               GAAGAC(2/6)
BsaAI        4260  CACGTG               [CT]AC^GT[GA]
BsiEI        1334  CGGCCG               CG[GA][CT]^CG
BsmI         2294  GCATTC               GAATGC(1/-1)
BsoBI        4462  CTCGAG               C^[CT]CG[GA]G
Bsp1407I     3551  TGTACA               T^GTACA
BsrGI        3551  TGTACA               T^GTACA
DrdI         2593  GACACTGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1334  CGGCCG               C^GGCCG
EclXI        1334  CGGCCG               C^GGCCG
Eco52I       1334  CGGCCG               C^GGCCG
HgaI          529  GACGC                GACGC(5/10)
MfeI          146  CAATTG               C^AATTG
MunI          146  CAATTG               C^AATTG
NaeI         5899  GCCGGC               GCC^GGC
NcoI         5815  CCATGG               C^CATGG
NdeI         3228  CATATG               CA^TATG
NgoMIV       5899  GCCGGC               G^CCGGC
PaeR7I       4462  CTCGAG               C^TCGAG
PmaCI        4260  CACGTG               CAC^GTG
PmlI         4260  CACGTG               CAC^GTG
PshBI         754  ATTAAT               AT^TAAT
Psp1406I     5771  AACGTT               AA^CGTT
SacI         3610  GAGCTC               GAGCT^C
SbfI         3288  CCTGCAGG             CCTGCA^GG
ScaI         3973  AGTACT               AGT^ACT
Sse8387I     3288  CCTGCAGG             CCTGCA^GG
SspBI        3551  TGTACA               T^GTACA
XbaI          643  TCTAGA               T^CTAGA
XhoI         4462  CTCGAG               C^TCGAG
XmnI          596  GAAGCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AccI         3846  GTCTAC               GT^[AC][GT]AC
             3930  GTAGAC              
AccII          12  CGCG                 CG^CG
             1633  CGCG                
AhdI         3829  GACCCTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4432  GACCCTATGTC         
ApaI         1853  GGGCCC               GGGCC^C
             2538  GGGCCC              
AspEI        3829  GACCCTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4432  GACCCTATGTC         
AspI         3332  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3933  GACTCTGTC           
BamHI        3490  GGATCC               G^GATCC
             4653  GGATCC              
BcnI          547  CCGGG                CC^[GC]GG
             2973  CCCGG               
BsaBI        2481  GATGCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2903  GATGATAATC          
BsaI          988  GAGACC               GGTCTC(1/5)
             1399  GGTCTC              
BsmBI         209  CGTCTC               CGTCTC(1/5)
              527  GAGACG              
BspHI        1655  TCATGA               T^CATGA
             5374  TCATGA              
BsrFI        4846  GCCGGT               [GA]^CCGG[CT]
             5899  GCCGGC              
BssSI        1547  CACGAG               CACGAG(-5/-1)
             2807  CTCGTG              
BstAPI       4044  GCAATTCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             5851  GCAGCTGCTGC         
BstUI          12  CGCG                 CG^CG
             1633  CGCG                
Cfr10I       4846  GCCGGT               [GA]^CCGG[CT]
             5899  GCCGGC              
DraI         5003  TTTAAA               TTT^AAA
             5626  TTTAAA              
DraIII       4155  CACGCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             5357  CACTTGGTG           
Eam1105I     3829  GACCCTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4432  GACCCTATGTC         
EcoRI        2801  GAATTC               G^AATTC
             5042  GAATTC              
HindIII      1538  AAGCTT               A^AGCTT
             5490  AAGCTT              
HpaI         2654  GTTAAC               GTT^AAC
             5032  GTTAAC              
MamI         2481  GATGCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2903  GATGATAATC          
MvnI           12  CGCG                 CG^CG
             1633  CGCG                
NciI          547  CCGGG                CC^[GC]GG
             2973  CCCGG               
PflFI        3332  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3933  GACTCTGTC           
RcaI         1655  TCATGA               T^CATGA
             5374  TCATGA              
SphI         3576  GCATGC               GCATG^C
             3920  GCATGC              
SspI         4949  AATATT               AAT^ATT
             4957  AATATT              
Tth111I      3332  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3933  GACTCTGTC           
XcmI         2874  CCACCAGGAATATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             4533  CCACAGGCCTGTTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  120 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
Alw44I      AlwI        AlwNI       AocI        ApaLI       
ApoI        AspHI       AvaII       AvrII       BalI        
BanI        BanII       BbvI        BfaI        BglI        
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BspLU11I    
BspMI       BsrDI       BsrI        BssKI       BstEII      
BstF5I      BstNI       BstPI       BstXI       BstYI       
Bsu36I      Cac8I       CelII       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HincII      
HindII      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PpuMI       PstI        PvuII       
RsaI        SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XhoII