################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## AatII Acc65I AccIII AcyI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AviII AvrII BbeI BbrPI BbvCI BcgI BfrI BglI BlnI BsaAI BsaHI BsaWI BseAI BsiEI BspDI BspEI BsrBI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstZ17I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I FseI FspI Hin1I KasI KpnI KspI MfeI MluI MroI MunI NarI NheI NotI NruI PacI PflFI PinAI PmaCI PmeI PmlI PvuI RsrII SacII SalI SfiI SfoI SgrAI SmaI SnaBI SpeI SspI Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccII 688 CGCG CG^CG AclI 3688 AACGTT AA^CGTT AocI 1115 CCTGAGG CC^T[ACGT]AGG AseI 6069 ATTAAT AT^TAAT AsnI 6069 ATTAAT AT^TAAT BamHI 2451 GGATCC G^GATCC BanI 1587 GGTGCC G^G[CT][GA]CC BcnI 4004 CCCGG CC^[GC]GG BsiWI 2823 CGTACG C^GTACG BsmBI 1010 GAGACG CGTCTC(1/5) BsrFI 844 GCCGGC [GA]^CCGG[CT] BstBI 55 TTCGAA TT^CGAA BstUI 688 CGCG CG^CG Bsu36I 1115 CCTGAGG CC^T[ACGT]AGG Cfr10I 844 GCCGGC [GA]^CCGG[CT] Eco81I 1115 CCTGAGG CC^T[ACGT]AGG HindIII 5750 AAGCTT A^AGCTT HpaI 1820 GTTAAC GTT^AAC MvnI 688 CGCG CG^CG NaeI 844 GCCGGC GCC^GGC NciI 4004 CCCGG CC^[GC]GG NdeI 4539 CATATG CA^TATG NgoMIV 844 GCCGGC G^CCGGC NspV 55 TTCGAA TT^CGAA PaeR7I 2714 CTCGAG C^TCGAG PshAI 5249 GACTCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 6069 ATTAAT AT^TAAT Psp1406I 3688 AACGTT AA^CGTT SacI 5415 GAGCTC GAGCT^C SbfI 2695 CCTGCAGG CCTGCA^GG ScaI 2496 AGTACT AGT^ACT SexAI 2535 ACCTGGT A^CC[AT]GGT SfuI 55 TTCGAA TT^CGAA Sse8387I 2695 CCTGCAGG CCTGCA^GG SwaI 4614 ATTTAAAT ATTT^AAAT XhoI 2714 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AflIII 1782 ACATGT A^C[GA][CT]GT 4329 ACATGT Asp700I 917 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5427 GAAGTTATTC AspHI 185 GTGCTC G[AT]GC[AT]^C 5415 GAGCTC AvaI 2714 CTCGAG C^[CT]CG[GA]G 3969 CCCGAG BanII 1760 GGGCTC G[GA]GC[CT]^C 5415 GAGCTC BbsI 477 GAAGAC GAAGAC(2/6) 4910 GAAGAC BciVI 4410 GTATCC GTATCC(6/5) 4869 GTATCC BclI 1877 TGATCA T^GATCA 6245 TGATCA BglII 452 AGATCT A^GATCT 570 AGATCT BpuAI 477 GAAGAC GAAGAC(2/6) 4910 GAAGAC BsiHKAI 185 GTGCTC G[AT]GC[AT]^C 5415 GAGCTC BsoBI 2714 CTCGAG C^[CT]CG[GA]G 3969 CCCGAG Bsp1407I 4332 TGTACA T^GTACA 4675 TGTACA BspHI 946 TCATGA T^CATGA 2001 TCATGA BspLU11I 1782 ACATGT A^CATGT 4329 ACATGT BsrGI 4332 TGTACA T^GTACA 4675 TGTACA DraIII 1252 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG 3236 CACACCGTG EcoNI 1415 CCTAAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3451 CCTGAATAAGG EcoRI 917 GAATTC G^AATTC 3680 GAATTC EcoRV 4747 GATATC GAT^ATC 5998 GATATC FbaI 1877 TGATCA T^GATCA 6245 TGATCA HapII 845 CCGG C^CGG 4005 CCGG HgaI 1602 GACGC GACGC(5/10) 4650 GCGTC HpaII 845 CCGG C^CGG 4005 CCGG MspI 845 CCGG C^CGG 4005 CCGG PflMI 1173 CCAAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2442 CCAGGAGCTGG PpuMI 2757 AGGACCT [GA]G^G[AT]CC[CT] 3335 AGGTCCT RcaI 946 TCATGA T^CATGA 2001 TCATGA SphI 1678 GCATGC GCATG^C 5124 GCATGC SspBI 4332 TGTACA T^GTACA 4675 TGTACA Van91I 1173 CCAAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2442 CCAGGAGCTGG XcmI 1421 CCAGGCAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4098 CCACACCATGCTTGG XmnI 917 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5427 GAAGTTATTC ######################################### # 3 OR MORE CUT ENZYMES ( 107 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI ApoI AvaII BalI BbvI BfaI BlpI BmyI BpmI Bpu1102I BsaBI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DrdI DsaI EaeI EarI EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HhaI HinP1I HincII HindII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MvaI MwoI NcoI NdeII NlaIII NlaIV NsiI NspI PleI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII