##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspI
AviII AvrII BbeI BbrPI BbvCI
BcgI BfrI BglI BlnI BsaAI
BsaHI BsaWI BseAI BsiEI BspDI
BspEI BsrBI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstZ17I ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I FseI FspI Hin1I
KasI KpnI KspI MfeI MluI
MroI MunI NarI NheI NotI
NruI PacI PflFI PinAI PmaCI
PmeI PmlI PvuI RsrII SacII
SalI SfiI SfoI SgrAI SmaI
SnaBI SpeI SspI Tth111I XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccII 688 CGCG CG^CG
AclI 3688 AACGTT AA^CGTT
AocI 1115 CCTGAGG CC^T[ACGT]AGG
AseI 6069 ATTAAT AT^TAAT
AsnI 6069 ATTAAT AT^TAAT
BamHI 2451 GGATCC G^GATCC
BanI 1587 GGTGCC G^G[CT][GA]CC
BcnI 4004 CCCGG CC^[GC]GG
BsiWI 2823 CGTACG C^GTACG
BsmBI 1010 GAGACG CGTCTC(1/5)
BsrFI 844 GCCGGC [GA]^CCGG[CT]
BstBI 55 TTCGAA TT^CGAA
BstUI 688 CGCG CG^CG
Bsu36I 1115 CCTGAGG CC^T[ACGT]AGG
Cfr10I 844 GCCGGC [GA]^CCGG[CT]
Eco81I 1115 CCTGAGG CC^T[ACGT]AGG
HindIII 5750 AAGCTT A^AGCTT
HpaI 1820 GTTAAC GTT^AAC
MvnI 688 CGCG CG^CG
NaeI 844 GCCGGC GCC^GGC
NciI 4004 CCCGG CC^[GC]GG
NdeI 4539 CATATG CA^TATG
NgoMIV 844 GCCGGC G^CCGGC
NspV 55 TTCGAA TT^CGAA
PaeR7I 2714 CTCGAG C^TCGAG
PshAI 5249 GACTCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 6069 ATTAAT AT^TAAT
Psp1406I 3688 AACGTT AA^CGTT
SacI 5415 GAGCTC GAGCT^C
SbfI 2695 CCTGCAGG CCTGCA^GG
ScaI 2496 AGTACT AGT^ACT
SexAI 2535 ACCTGGT A^CC[AT]GGT
SfuI 55 TTCGAA TT^CGAA
Sse8387I 2695 CCTGCAGG CCTGCA^GG
SwaI 4614 ATTTAAAT ATTT^AAAT
XhoI 2714 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AflIII 1782 ACATGT A^C[GA][CT]GT
4329 ACATGT
Asp700I 917 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5427 GAAGTTATTC
AspHI 185 GTGCTC G[AT]GC[AT]^C
5415 GAGCTC
AvaI 2714 CTCGAG C^[CT]CG[GA]G
3969 CCCGAG
BanII 1760 GGGCTC G[GA]GC[CT]^C
5415 GAGCTC
BbsI 477 GAAGAC GAAGAC(2/6)
4910 GAAGAC
BciVI 4410 GTATCC GTATCC(6/5)
4869 GTATCC
BclI 1877 TGATCA T^GATCA
6245 TGATCA
BglII 452 AGATCT A^GATCT
570 AGATCT
BpuAI 477 GAAGAC GAAGAC(2/6)
4910 GAAGAC
BsiHKAI 185 GTGCTC G[AT]GC[AT]^C
5415 GAGCTC
BsoBI 2714 CTCGAG C^[CT]CG[GA]G
3969 CCCGAG
Bsp1407I 4332 TGTACA T^GTACA
4675 TGTACA
BspHI 946 TCATGA T^CATGA
2001 TCATGA
BspLU11I 1782 ACATGT A^CATGT
4329 ACATGT
BsrGI 4332 TGTACA T^GTACA
4675 TGTACA
DraIII 1252 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
3236 CACACCGTG
EcoNI 1415 CCTAAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3451 CCTGAATAAGG
EcoRI 917 GAATTC G^AATTC
3680 GAATTC
EcoRV 4747 GATATC GAT^ATC
5998 GATATC
FbaI 1877 TGATCA T^GATCA
6245 TGATCA
HapII 845 CCGG C^CGG
4005 CCGG
HgaI 1602 GACGC GACGC(5/10)
4650 GCGTC
HpaII 845 CCGG C^CGG
4005 CCGG
MspI 845 CCGG C^CGG
4005 CCGG
PflMI 1173 CCAAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2442 CCAGGAGCTGG
PpuMI 2757 AGGACCT [GA]G^G[AT]CC[CT]
3335 AGGTCCT
RcaI 946 TCATGA T^CATGA
2001 TCATGA
SphI 1678 GCATGC GCATG^C
5124 GCATGC
SspBI 4332 TGTACA T^GTACA
4675 TGTACA
Van91I 1173 CCAAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2442 CCAGGAGCTGG
XcmI 1421 CCAGGCAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4098 CCACACCATGCTTGG
XmnI 917 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5427 GAAGTTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AvaII BalI
BbvI BfaI BlpI BmyI BpmI
Bpu1102I BsaBI BsaI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII DrdI DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeII
HaeIII HhaI HinP1I HincII HindII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MvaI MwoI NcoI
NdeII NlaIII NlaIV NsiI NspI
PleI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII