################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AatII AccIII AgeI Aor51HI AscI AviII AvrII BalI BbeI BbrPI BlnI BsaAI BsaI BseAI BsiEI BsiWI BspDI BspEI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Cfr10I ClaI CpoI DraIII EagI EclXI Eco47III Eco52I EcoO65I EcoRV FauI FseI FspI KasI KspI MluI MluNI MroI MscI NaeI NarI NgoMIV NotI NruI NspV PacI PinAI PmaCI PmlI PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SwaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### Acc65I 1309 GGTACC G^GTACC AccI 285 GTCTAC GT^[AC][GT]AC AccII 513 CGCG CG^CG AflIII 2439 ACATGT A^C[GA][CT]GT AhdI 856 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 737 GTGCAC G^TGCAC ApaI 2484 GGGCCC GGGCC^C ApaLI 737 GTGCAC G^TGCAC AseI 4692 ATTAAT AT^TAAT AsnI 4692 ATTAAT AT^TAAT Asp718I 1309 GGTACC G^GTACC AspEI 856 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 502 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 573 GGATCC G^GATCC BanI 1309 GGTACC G^G[CT][GA]CC BbvCI 2590 CCTCAGC CCTCAGC(-5/-2) BcgI 1282 CGACTCCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 3849 GTATCC GTATCC(6/5) BcnI 1859 CCCGG CC^[GC]GG BglI 1424 GCCATAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 3505 AGATCT A^GATCT BlpI 1729 GCTAAGC GC^T[ACGT]AGC Bpu1102I 1729 GCTAAGC GC^T[ACGT]AGC BsaBI 2266 GATAGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 2039 ACCGGA [AT]^CCGG[AT] BspHI 4952 TCATGA T^CATGA BspLU11I 2439 ACATGT A^CATGT BstUI 513 CGCG CG^CG BstXI 2481 CCAGGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1729 GCTAAGC GC^T[ACGT]AGC EaeI 1837 CGGCCA [CT]^GGCC[GA] Eam1105I 856 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 4263 CCTCAAATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 5103 ATGCAT ATGCA^T HaeII 3066 GGCGCT [GA]GCGC^[CT] HindIII 2314 AAGCTT A^AGCTT HpaI 4603 GTTAAC GTT^AAC KpnI 1309 GGTACC GGTAC^C MamI 2266 GATAGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1314 CAATTG C^AATTG MunI 1314 CAATTG C^AATTG MvnI 513 CGCG CG^CG NciI 1859 CCCGG CC^[GC]GG NcoI 2402 CCATGG C^CATGG NheI 234 GCTAGC G^CTAGC NsiI 5103 ATGCAT ATGCA^T PaeR7I 1690 CTCGAG C^TCGAG PflFI 502 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 3664 CCACGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmeI 4389 GTTTAAAC GTTT^AAAC PpuMI 4016 AGGTCCT [GA]G^G[AT]CC[CT] PshBI 4692 ATTAAT AT^TAAT RcaI 4952 TCATGA T^CATGA SacI 1255 GAGCTC GAGCT^C SapI 1012 GAAGAGC GCTCTTC(1/4) SpeI 607 ACTAGT A^CTAGT StuI 2228 AGGCCT AGG^CCT Tth111I 502 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 3664 CCACGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 1690 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AcyI 478 GACGCC G[GA]^CG[CT]C 2841 GACGCC AocI 530 CCTCAGG CC^T[ACGT]AGG 3119 CCTCAGG Asp700I 112 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1234 GAAGAGATTC AvaI 838 CCCGAG C^[CT]CG[GA]G 1690 CTCGAG BanII 1255 GAGCTC G[GA]GC[CT]^C 2484 GGGCCC BsaHI 478 GACGCC G[GA]^CG[CT]C 2841 GACGCC BsgI 1893 GTGCAG GTGCAG(16/14) 4937 GTGCAG BsiYI 3664 CCACGGACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 4263 CCTCAAATAGG BslI 3664 CCACGGACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 4263 CCTCAAATAGG BsmBI 497 GAGACG CGTCTC(1/5) 616 GAGACG BsmI 1420 GAATGC GAATGC(1/-1) 1623 GCATTC BsoBI 838 CCCGAG C^[CT]CG[GA]G 1690 CTCGAG Bsp1407I 1033 TGTACA T^GTACA 4418 TGTACA BsrDI 1869 CATTGC GCAATG(2/0) 1043 GCAATG BsrGI 1033 TGTACA T^GTACA 4418 TGTACA Bsu36I 530 CCTCAGG CC^T[ACGT]AGG 3119 CCTCAGG CfoI 512 GCGC GCG^C 3067 GCGC Eco81I 530 CCTCAGG CC^T[ACGT]AGG 3119 CCTCAGG EcoRI 411 GAATTC G^AATTC 3336 GAATTC EcoT14I 2402 CCATGG C^C[AT][AT]GG 5095 CCAAGG HapII 1860 CCGG C^CGG 2040 CCGG HhaI 512 GCGC GCG^C 3067 GCGC Hin1I 478 GACGCC G[GA]^CG[CT]C 2841 GACGCC HinP1I 512 GCGC G^CGC 3067 GCGC HpaII 1860 CCGG C^CGG 2040 CCGG MspI 1860 CCGG C^CGG 2040 CCGG PshAI 2425 GACCTTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3599 GACCATTGTC PstI 224 CTGCAG CTGCA^G 3341 CTGCAG ScaI 1006 AGTACT AGT^ACT 4278 AGTACT SspBI 1033 TGTACA T^GTACA 4418 TGTACA StyI 2402 CCATGG C^C[AT][AT]GG 5095 CCAAGG XbaI 657 TCTAGA T^CTAGA 4290 TCTAGA XmnI 112 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1234 GAAGAGATTC ######################################### # 3 OR MORE CUT ENZYMES ( 90 enzymes) # ######################################### AciI AclI AcsI AfaI AflII AluI AlwI AlwNI ApoI AspHI AvaII BbsI BbvI BclI BfaI BfrI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII DrdI DsaI EarI EcoO109I EcoRII FbaI Fnu4HI FokI HaeIII HgaI HincII HindII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MvaI MwoI NdeI NdeII NlaIII NlaIV NspI PleI Psp1406I PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII