##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AccIII AgeI Aor51HI AscI
AviII AvrII BalI BbeI BbrPI
BlnI BsaAI BsaI BseAI BsiEI
BsiWI BspDI BspEI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Cfr10I ClaI
CpoI DraIII EagI EclXI Eco47III
Eco52I EcoO65I EcoRV FauI FseI
FspI KasI KspI MluI MluNI
MroI MscI NaeI NarI NgoMIV
NotI NruI NspV PacI PinAI
PmaCI PmlI PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SwaI XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
Acc65I 1309 GGTACC G^GTACC
AccI 285 GTCTAC GT^[AC][GT]AC
AccII 513 CGCG CG^CG
AflIII 2439 ACATGT A^C[GA][CT]GT
AhdI 856 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 737 GTGCAC G^TGCAC
ApaI 2484 GGGCCC GGGCC^C
ApaLI 737 GTGCAC G^TGCAC
AseI 4692 ATTAAT AT^TAAT
AsnI 4692 ATTAAT AT^TAAT
Asp718I 1309 GGTACC G^GTACC
AspEI 856 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 502 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 573 GGATCC G^GATCC
BanI 1309 GGTACC G^G[CT][GA]CC
BbvCI 2590 CCTCAGC CCTCAGC(-5/-2)
BcgI 1282 CGACTCCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3849 GTATCC GTATCC(6/5)
BcnI 1859 CCCGG CC^[GC]GG
BglI 1424 GCCATAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3505 AGATCT A^GATCT
BlpI 1729 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1729 GCTAAGC GC^T[ACGT]AGC
BsaBI 2266 GATAGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2039 ACCGGA [AT]^CCGG[AT]
BspHI 4952 TCATGA T^CATGA
BspLU11I 2439 ACATGT A^CATGT
BstUI 513 CGCG CG^CG
BstXI 2481 CCAGGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1729 GCTAAGC GC^T[ACGT]AGC
EaeI 1837 CGGCCA [CT]^GGCC[GA]
Eam1105I 856 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 4263 CCTCAAATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 5103 ATGCAT ATGCA^T
HaeII 3066 GGCGCT [GA]GCGC^[CT]
HindIII 2314 AAGCTT A^AGCTT
HpaI 4603 GTTAAC GTT^AAC
KpnI 1309 GGTACC GGTAC^C
MamI 2266 GATAGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1314 CAATTG C^AATTG
MunI 1314 CAATTG C^AATTG
MvnI 513 CGCG CG^CG
NciI 1859 CCCGG CC^[GC]GG
NcoI 2402 CCATGG C^CATGG
NheI 234 GCTAGC G^CTAGC
NsiI 5103 ATGCAT ATGCA^T
PaeR7I 1690 CTCGAG C^TCGAG
PflFI 502 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3664 CCACGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmeI 4389 GTTTAAAC GTTT^AAAC
PpuMI 4016 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 4692 ATTAAT AT^TAAT
RcaI 4952 TCATGA T^CATGA
SacI 1255 GAGCTC GAGCT^C
SapI 1012 GAAGAGC GCTCTTC(1/4)
SpeI 607 ACTAGT A^CTAGT
StuI 2228 AGGCCT AGG^CCT
Tth111I 502 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3664 CCACGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1690 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 478 GACGCC G[GA]^CG[CT]C
2841 GACGCC
AocI 530 CCTCAGG CC^T[ACGT]AGG
3119 CCTCAGG
Asp700I 112 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1234 GAAGAGATTC
AvaI 838 CCCGAG C^[CT]CG[GA]G
1690 CTCGAG
BanII 1255 GAGCTC G[GA]GC[CT]^C
2484 GGGCCC
BsaHI 478 GACGCC G[GA]^CG[CT]C
2841 GACGCC
BsgI 1893 GTGCAG GTGCAG(16/14)
4937 GTGCAG
BsiYI 3664 CCACGGACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
4263 CCTCAAATAGG
BslI 3664 CCACGGACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
4263 CCTCAAATAGG
BsmBI 497 GAGACG CGTCTC(1/5)
616 GAGACG
BsmI 1420 GAATGC GAATGC(1/-1)
1623 GCATTC
BsoBI 838 CCCGAG C^[CT]CG[GA]G
1690 CTCGAG
Bsp1407I 1033 TGTACA T^GTACA
4418 TGTACA
BsrDI 1869 CATTGC GCAATG(2/0)
1043 GCAATG
BsrGI 1033 TGTACA T^GTACA
4418 TGTACA
Bsu36I 530 CCTCAGG CC^T[ACGT]AGG
3119 CCTCAGG
CfoI 512 GCGC GCG^C
3067 GCGC
Eco81I 530 CCTCAGG CC^T[ACGT]AGG
3119 CCTCAGG
EcoRI 411 GAATTC G^AATTC
3336 GAATTC
EcoT14I 2402 CCATGG C^C[AT][AT]GG
5095 CCAAGG
HapII 1860 CCGG C^CGG
2040 CCGG
HhaI 512 GCGC GCG^C
3067 GCGC
Hin1I 478 GACGCC G[GA]^CG[CT]C
2841 GACGCC
HinP1I 512 GCGC G^CGC
3067 GCGC
HpaII 1860 CCGG C^CGG
2040 CCGG
MspI 1860 CCGG C^CGG
2040 CCGG
PshAI 2425 GACCTTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3599 GACCATTGTC
PstI 224 CTGCAG CTGCA^G
3341 CTGCAG
ScaI 1006 AGTACT AGT^ACT
4278 AGTACT
SspBI 1033 TGTACA T^GTACA
4418 TGTACA
StyI 2402 CCATGG C^C[AT][AT]GG
5095 CCAAGG
XbaI 657 TCTAGA T^CTAGA
4290 TCTAGA
XmnI 112 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1234 GAAGAGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AclI AcsI AfaI AflII
AluI AlwI AlwNI ApoI AspHI
AvaII BbsI BbvI BclI BfaI
BfrI BmyI BpmI BpuAI BsaJI
BseRI BsiHKAI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraII DrdI DsaI EarI
EcoO109I EcoRII FbaI Fnu4HI FokI
HaeIII HgaI HincII HindII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MvaI MwoI
NdeI NdeII NlaIII NlaIV NspI
PleI Psp1406I PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII