################################## # NO CUT ENZYMES ( 58 enzymes) # ################################## AccI AccIII AclI AflII AscI AseI AsnI AviII BbvCI BclI BfrI BsaBI BseAI BsiWI Bsp1407I BspDI BspEI BsrGI BssHII Bst1107I BstBI BstZ17I ClaI DrdI EagI EclXI Eco52I EcoRI EcoRV FbaI FseI FspI KspI MamI MfeI MluI MroI MunI NcoI NheI NotI NruI NspV PacI PmeI PshBI Psp1406I PvuI SacII SalI SexAI SfiI SfuI SnaBI SpeI SspBI SspI SwaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AatII 2052 GACGTC GACGT^C Acc65I 2445 GGTACC G^GTACC AccII 539 CGCG CG^CG AhdI 2550 GACACAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 1274 AGCGCT AGC^GCT Asp700I 1602 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2445 GGTACC G^GTACC AspEI 2550 GACACAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 1579 TGGCCA TGG^CCA BamHI 2354 GGATCC G^GATCC BbeI 3166 GGCGCC GGCGC^C BciVI 681 GTATCC GTATCC(6/5) BglII 4501 AGATCT A^GATCT BsiEI 4626 CGGTCG CG[GA][CT]^CG BspHI 4591 TCATGA T^CATGA BssSI 3844 CTCGTG CACGAG(-5/-1) BstUI 539 CGCG CG^CG BstXI 649 CCAGCGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CpoI 841 CGGACCG CG^G[AT]CCG Eam1105I 2550 GACACAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1274 AGCGCT AGC^GCT EcoT22I 2589 ATGCAT ATGCA^T HindIII 1005 AAGCTT A^AGCTT HpaI 3438 GTTAAC GTT^AAC KasI 3166 GGCGCC G^GCGCC KpnI 2445 GGTACC GGTAC^C MluNI 1579 TGGCCA TGG^CCA MscI 1579 TGGCCA TGG^CCA MvnI 539 CGCG CG^CG NaeI 1308 GCCGGC GCC^GGC NarI 3166 GGCGCC GG^CGCC NdeI 2466 CATATG CA^TATG NgoMIV 1308 GCCGGC G^CCGGC NsiI 2589 ATGCAT ATGCA^T RcaI 4591 TCATGA T^CATGA RsrII 841 CGGACCG CG^G[AT]CCG SapI 2291 GCTCTTC GCTCTTC(1/4) ScaI 4228 AGTACT AGT^ACT SfoI 3166 GGCGCC GGC^GCC SmaI 1705 CCCGGG CCC^GGG XbaI 3837 TCTAGA T^CTAGA XmaCI 1705 CCCGGG C^CCGGG XmaI 1705 CCCGGG C^CCGGG XmnI 1602 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AcsI 2244 AAATTT [GA]^AATT[CT] 2618 AAATTC AcyI 2052 GACGTC G[GA]^CG[CT]C 3166 GGCGCC AgeI 1681 ACCGGT A^CCGGT 2563 ACCGGT Alw44I 2010 GTGCAC G^TGCAC 3885 GTGCAC ApaLI 2010 GTGCAC G^TGCAC 3885 GTGCAC ApoI 2244 AAATTT [GA]^AATT[CT] 2618 AAATTC AvrII 1239 CCTAGG C^CTAGG 2193 CCTAGG BbrPI 2544 CACGTG CAC^GTG 2694 CACGTG BcgI 3553 CGACACGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1481 GCACTCTCCTCG BlnI 1239 CCTAGG C^CTAGG 2193 CCTAGG BsaHI 2052 GACGTC G[GA]^CG[CT]C 3166 GGCGCC BsaI 1035 GAGACC GGTCTC(1/5) 3061 GGTCTC BsmI 191 GAATGC GAATGC(1/-1) 1927 GCATTC BspLU11I 1733 ACATGT A^CATGT 3889 ACATGT BsrBI 2074 CCGCTC CCGCTC(-3/-3) 2767 GAGCGG DraI 2209 TTTAAA TTT^AAA 2673 TTTAAA DsaI 1994 CCACGG C^C[GA][CT]GG 2834 CCGTGG HaeII 1274 AGCGCT [GA]GCGC^[CT] 3166 GGCGCC HgaI 1475 GACGC GACGC(5/10) 3082 GACGC Hin1I 2052 GACGTC G[GA]^CG[CT]C 3166 GGCGCC HincII 3438 GTTAAC GT[CT]^[GA]AC 4347 GTTGAC HindII 3438 GTTAAC GT[CT]^[GA]AC 4347 GTTGAC PinAI 1681 ACCGGT A^CCGGT 2563 ACCGGT PmaCI 2544 CACGTG CAC^GTG 2694 CACGTG PmlI 2544 CACGTG CAC^GTG 2694 CACGTG PshAI 1037 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1739 GACTGCTGTC SacI 2225 GAGCTC GAGCT^C 2839 GAGCTC SgrAI 2562 CACCGGTG C[GA]^CCGG[CT]G 3168 CGCCGGTG SphI 3468 GCATGC GCATG^C 4269 GCATGC StuI 3669 AGGCCT AGG^CCT 4404 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 126 enzymes) # ######################################### AciI AfaI AflIII AluI AlwI AlwNI AocI ApaI AspHI AspI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII EaeI EarI Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PaeR7I PflFI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I SbfI ScrFI SfaNI SfcI SmlI Sse8387I StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoI XhoII