##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AccI AccIII AclI AflII AscI
AseI AsnI AviII BbvCI BclI
BfrI BsaBI BseAI BsiWI Bsp1407I
BspDI BspEI BsrGI BssHII Bst1107I
BstBI BstZ17I ClaI DrdI EagI
EclXI Eco52I EcoRI EcoRV FbaI
FseI FspI KspI MamI MfeI
MluI MroI MunI NcoI NheI
NotI NruI NspV PacI PmeI
PshBI Psp1406I PvuI SacII SalI
SexAI SfiI SfuI SnaBI SpeI
SspBI SspI SwaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 2052 GACGTC GACGT^C
Acc65I 2445 GGTACC G^GTACC
AccII 539 CGCG CG^CG
AhdI 2550 GACACAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1274 AGCGCT AGC^GCT
Asp700I 1602 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2445 GGTACC G^GTACC
AspEI 2550 GACACAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1579 TGGCCA TGG^CCA
BamHI 2354 GGATCC G^GATCC
BbeI 3166 GGCGCC GGCGC^C
BciVI 681 GTATCC GTATCC(6/5)
BglII 4501 AGATCT A^GATCT
BsiEI 4626 CGGTCG CG[GA][CT]^CG
BspHI 4591 TCATGA T^CATGA
BssSI 3844 CTCGTG CACGAG(-5/-1)
BstUI 539 CGCG CG^CG
BstXI 649 CCAGCGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 841 CGGACCG CG^G[AT]CCG
Eam1105I 2550 GACACAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1274 AGCGCT AGC^GCT
EcoT22I 2589 ATGCAT ATGCA^T
HindIII 1005 AAGCTT A^AGCTT
HpaI 3438 GTTAAC GTT^AAC
KasI 3166 GGCGCC G^GCGCC
KpnI 2445 GGTACC GGTAC^C
MluNI 1579 TGGCCA TGG^CCA
MscI 1579 TGGCCA TGG^CCA
MvnI 539 CGCG CG^CG
NaeI 1308 GCCGGC GCC^GGC
NarI 3166 GGCGCC GG^CGCC
NdeI 2466 CATATG CA^TATG
NgoMIV 1308 GCCGGC G^CCGGC
NsiI 2589 ATGCAT ATGCA^T
RcaI 4591 TCATGA T^CATGA
RsrII 841 CGGACCG CG^G[AT]CCG
SapI 2291 GCTCTTC GCTCTTC(1/4)
ScaI 4228 AGTACT AGT^ACT
SfoI 3166 GGCGCC GGC^GCC
SmaI 1705 CCCGGG CCC^GGG
XbaI 3837 TCTAGA T^CTAGA
XmaCI 1705 CCCGGG C^CCGGG
XmaI 1705 CCCGGG C^CCGGG
XmnI 1602 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 2244 AAATTT [GA]^AATT[CT]
2618 AAATTC
AcyI 2052 GACGTC G[GA]^CG[CT]C
3166 GGCGCC
AgeI 1681 ACCGGT A^CCGGT
2563 ACCGGT
Alw44I 2010 GTGCAC G^TGCAC
3885 GTGCAC
ApaLI 2010 GTGCAC G^TGCAC
3885 GTGCAC
ApoI 2244 AAATTT [GA]^AATT[CT]
2618 AAATTC
AvrII 1239 CCTAGG C^CTAGG
2193 CCTAGG
BbrPI 2544 CACGTG CAC^GTG
2694 CACGTG
BcgI 3553 CGACACGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1481 GCACTCTCCTCG
BlnI 1239 CCTAGG C^CTAGG
2193 CCTAGG
BsaHI 2052 GACGTC G[GA]^CG[CT]C
3166 GGCGCC
BsaI 1035 GAGACC GGTCTC(1/5)
3061 GGTCTC
BsmI 191 GAATGC GAATGC(1/-1)
1927 GCATTC
BspLU11I 1733 ACATGT A^CATGT
3889 ACATGT
BsrBI 2074 CCGCTC CCGCTC(-3/-3)
2767 GAGCGG
DraI 2209 TTTAAA TTT^AAA
2673 TTTAAA
DsaI 1994 CCACGG C^C[GA][CT]GG
2834 CCGTGG
HaeII 1274 AGCGCT [GA]GCGC^[CT]
3166 GGCGCC
HgaI 1475 GACGC GACGC(5/10)
3082 GACGC
Hin1I 2052 GACGTC G[GA]^CG[CT]C
3166 GGCGCC
HincII 3438 GTTAAC GT[CT]^[GA]AC
4347 GTTGAC
HindII 3438 GTTAAC GT[CT]^[GA]AC
4347 GTTGAC
PinAI 1681 ACCGGT A^CCGGT
2563 ACCGGT
PmaCI 2544 CACGTG CAC^GTG
2694 CACGTG
PmlI 2544 CACGTG CAC^GTG
2694 CACGTG
PshAI 1037 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1739 GACTGCTGTC
SacI 2225 GAGCTC GAGCT^C
2839 GAGCTC
SgrAI 2562 CACCGGTG C[GA]^CCGG[CT]G
3168 CGCCGGTG
SphI 3468 GCATGC GCATG^C
4269 GCATGC
StuI 3669 AGGCCT AGG^CCT
4404 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI AocI ApaI AspHI AspI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII EaeI EarI
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PaeR7I PflFI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I SbfI ScrFI SfaNI SfcI
SmlI Sse8387I StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoI
XhoII