##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI AocI Aor51HI
ApaI AscI Asp718I AspEI AviII
AvrII BamHI BbrPI BcgI BciVI
BclI BfrI BglI BlnI BsaAI
BsaWI BseAI BsiEI BsiWI BsmBI
BspEI BsrFI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cfr10I CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV FbaI FseI
FspI KpnI KspI MluI MroI
MvnI NaeI NdeI NgoMIV NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmlI PshAI Psp1406I PvuI
RsrII SacII SalI SbfI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AflIII 2817 ACATGT A^C[GA][CT]GT
Asp700I 4152 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 3788 CCCGGG C^[CT]CG[GA]G
BbeI 409 GGCGCC GGCGC^C
BsaBI 2622 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 2947 GAATGC GAATGC(1/-1)
BsoBI 3788 CCCGGG C^[CT]CG[GA]G
Bsp1407I 2514 TGTACA T^GTACA
BspDI 1460 ATCGAT AT^CGAT
BspLU11I 2817 ACATGT A^CATGT
BsrBI 2419 GAGCGG CCGCTC(-3/-3)
BsrGI 2514 TGTACA T^GTACA
BssSI 3228 CTCGTG CACGAG(-5/-1)
ClaI 1460 ATCGAT AT^CGAT
DsaI 2306 CCATGG C^C[GA][CT]GG
EcoNI 4652 CCTTGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 2795 ATGCAT ATGCA^T
HaeII 409 GGCGCC [GA]GCGC^[CT]
HincII 853 GTTAAC GT[CT]^[GA]AC
HindII 853 GTTAAC GT[CT]^[GA]AC
HindIII 2275 AAGCTT A^AGCTT
HpaI 853 GTTAAC GTT^AAC
KasI 409 GGCGCC G^GCGCC
MaeII 4068 ACGT A^CGT
MamI 2622 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2600 CAATTG C^AATTG
MspA1I 5 CAGCTG C[AC]G^C[GT]G
MunI 2600 CAATTG C^AATTG
NarI 409 GGCGCC GG^CGCC
NcoI 2306 CCATGG C^CATGG
NheI 2482 GCTAGC G^CTAGC
NsiI 2795 ATGCAT ATGCA^T
PflMI 1437 CCAACTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmeI 804 GTTTAAAC GTTT^AAAC
PstI 3665 CTGCAG CTGCA^G
PvuII 5 CAGCTG CAG^CTG
SapI 4074 GCTCTTC GCTCTTC(1/4)
ScaI 3245 AGTACT AGT^ACT
SfoI 409 GGCGCC GGC^GCC
SmaI 3788 CCCGGG CCC^GGG
SspBI 2514 TGTACA T^GTACA
Van91I 1437 CCAACTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 3788 CCCGGG C^CCGGG
XmaI 3788 CCCGGG C^CCGGG
XmnI 4152 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 2386 GTAGAC GT^[AC][GT]AC
4767 GTCTAC
AcyI 409 GGCGCC G[GA]^CG[CT]C
503 GACGCC
AspI 2333 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4584 GACACTGTC
BalI 2357 TGGCCA TGG^CCA
4698 TGGCCA
BbvCI 4555 CCTCAGC CCTCAGC(-5/-2)
2231 GCTGAGG
BglII 717 AGATCT A^GATCT
2627 AGATCT
BlpI 551 GCTCAGC GC^T[ACGT]AGC
2590 GCTTAGC
Bpu1102I 551 GCTCAGC GC^T[ACGT]AGC
2590 GCTTAGC
BsaHI 409 GGCGCC G[GA]^CG[CT]C
503 GACGCC
BspHI 1664 TCATGA T^CATGA
3799 TCATGA
BsrDI 168 GCAATG GCAATG(2/0)
1755 GCAATG
BstAPI 307 GCAGAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2958 GCAGATCATGC
CelII 551 GCTCAGC GC^T[ACGT]AGC
2590 GCTTAGC
CfoI 410 GCGC GCG^C
2855 GCGC
FauI 445 CCCGC CCCGC(4/6)
2421 GCGGG
HgaI 503 GACGC GACGC(5/10)
3680 GACGC
HhaI 410 GCGC GCG^C
2855 GCGC
Hin1I 409 GGCGCC G[GA]^CG[CT]C
503 GACGCC
HinP1I 410 GCGC G^CGC
2855 GCGC
MluNI 2357 TGGCCA TGG^CCA
4698 TGGCCA
MscI 2357 TGGCCA TGG^CCA
4698 TGGCCA
MslI 4006 CACTCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4586 CACTGTCATG
PflFI 2333 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4584 GACACTGTC
RcaI 1664 TCATGA T^CATGA
3799 TCATGA
SacI 391 GAGCTC GAGCT^C
2170 GAGCTC
SexAI 477 ACCAGGT A^CC[AT]GGT
893 ACCAGGT
SmlI 212 CTTGAG C^T[CT][GA]AG
3199 CTTGAG
SphI 2595 GCATGC GCATG^C
2797 GCATGC
Tth111I 2333 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4584 GACACTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI AlwNI ApaLI ApoI AseI
AsnI AspHI AvaII BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI DraII EaeI
EarI EcoO109I EcoRI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PshBI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII