##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AclI AgeI Alw44I ApaI
ApaLI AscI AseI AsnI Asp700I
BciVI BglII BsaBI BsiWI BspDI
BspHI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI EcoRI EcoRV EcoT22I
FseI HindIII HpaI MamI MfeI
MunI NaeI NdeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI ScaI
SfuI SgrAI SnaBI SpeI SspI
SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 2786 GGTACC G^GTACC
AccIII 1681 TCCGGA T^CCGGA
AhdI 375 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 603 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2041 AGCGCT AGC^GCT
Asp718I 2786 GGTACC G^GTACC
AspEI 375 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4716 CCTAGG C^CTAGG
BalI 3759 TGGCCA TGG^CCA
BamHI 1712 GGATCC G^GATCC
BbeI 2273 GGCGCC GGCGC^C
BcgI 2498 CGAAGAGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 199 TGATCA T^GATCA
BlnI 4716 CCTAGG C^CTAGG
BsaWI 1681 TCCGGA [AT]^CCGG[AT]
BseAI 1681 TCCGGA T^CCGGA
BsiEI 2494 CGGCCG CG[GA][CT]^CG
BsmBI 2670 GAGACG CGTCTC(1/5)
BsmI 2504 GAATGC GAATGC(1/-1)
Bsp1407I 3603 TGTACA T^GTACA
BspEI 1681 TCCGGA T^CCGGA
BspLU11I 2358 ACATGT A^CATGT
BsrGI 3603 TGTACA T^GTACA
BssSI 4686 CTCGTG CACGAG(-5/-1)
BstEII 1986 GGTGACC G^GT[ACGT]ACC
BstPI 1986 GGTGACC G^GT[ACGT]ACC
Bsu36I 603 CCTCAGG CC^T[ACGT]AGG
DraI 4914 TTTAAA TTT^AAA
DrdI 2410 GACACTCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2494 CGGCCG C^GGCCG
Eam1105I 375 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2494 CGGCCG C^GGCCG
Eco47III 2041 AGCGCT AGC^GCT
Eco52I 2494 CGGCCG C^GGCCG
Eco81I 603 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1986 GGTGACC G^GT[ACGT]ACC
FbaI 199 TGATCA T^GATCA
KasI 2273 GGCGCC G^GCGCC
KpnI 2786 GGTACC GGTAC^C
KspI 2081 CCGCGG CCGC^GG
MluI 780 ACGCGT A^CGCGT
MluNI 3759 TGGCCA TGG^CCA
MroI 1681 TCCGGA T^CCGGA
MscI 3759 TGGCCA TGG^CCA
NarI 2273 GGCGCC GG^CGCC
SacII 2081 CCGCGG CCGC^GG
SapI 5030 GCTCTTC GCTCTTC(1/4)
SexAI 859 ACCTGGT A^CC[AT]GGT
SfiI 3083 GGCCACAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2273 GGCGCC GGC^GCC
SspBI 3603 TGTACA T^GTACA
XcmI 2738 CCAGTGGACACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 4168 GTAGAC GT^[AC][GT]AC
4751 GTCTAC
AcyI 87 GACGCC G[GA]^CG[CT]C
2273 GGCGCC
AspI 858 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4195 GACAAAGTC
AviII 2185 TGCGCA TGC^GCA
4770 TGCGCA
BbvCI 5201 CCTCAGC CCTCAGC(-5/-2)
4184 GCTGAGG
BlpI 1232 GCTGAGC GC^T[ACGT]AGC
2055 GCTGAGC
Bpu1102I 1232 GCTGAGC GC^T[ACGT]AGC
2055 GCTGAGC
BsaHI 87 GACGCC G[GA]^CG[CT]C
2273 GGCGCC
BsrBI 3809 CCGCTC CCGCTC(-3/-3)
1431 GAGCGG
BsrDI 1382 CATTGC GCAATG(2/0)
2761 GCAATG
BstAPI 767 GCAGCACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2511 GCATCCAGTGC
BstXI 64 CCAAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2515 CCAGTGCTCTGG
CelII 1232 GCTGAGC GC^T[ACGT]AGC
2055 GCTGAGC
FspI 2185 TGCGCA TGC^GCA
4770 TGCGCA
Hin1I 87 GACGCC G[GA]^CG[CT]C
2273 GGCGCC
NcoI 1729 CCATGG C^CATGG
3547 CCATGG
NheI 2603 GCTAGC G^CTAGC
3709 GCTAGC
PflFI 858 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4195 GACAAAGTC
SmaI 748 CCCGGG CCC^GGG
1293 CCCGGG
SphI 595 GCATGC GCATG^C
1021 GCATGC
StuI 3346 AGGCCT AGG^CCT
3950 AGGCCT
TaqI 644 TCGA T^CGA
5149 TCGA
Tth111I 858 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4195 GACAAAGTC
TthHB8I 644 TCGA T^CGA
5149 TCGA
XmaCI 748 CCCGGG C^CCGGG
1293 CCCGGG
XmaI 748 CCCGGG C^CCGGG
1293 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AflIII AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BanI BanII
BbrPI BbsI BbvI BcnI BfaI
BfrI BglI BmyI BpmI BpuAI
BsaAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I SbfI ScrFI SfaNI SfcI
SmlI Sse8387I StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XhoII