##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
Acc65I AccIII AclI AgeI AlwNI
AscI Asp718I AspI AviII AvrII
BbeI BbrPI BbvCI BglI BlnI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BspLU11I BsrFI BssHII BstBI
BstEII BstPI Cfr10I ClaI CpoI
DraIII EagI EclXI Eco52I EcoNI
EcoO65I EcoRV FseI FspI HindIII
KasI KpnI KspI MluI MroI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PstI PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I StuI SwaI
Tth111I Van91I XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 4128 GACGTC GACGT^C
AccII 4313 CGCG CG^CG
AcyI 4128 GACGTC G[GA]^CG[CT]C
AflII 3402 CTTAAG C^TTAAG
AhdI 349 GACCAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3340 GTGCAC G^TGCAC
ApaI 386 GGGCCC GGGCC^C
ApaLI 3340 GTGCAC G^TGCAC
AspEI 349 GACCAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2201 CCCGAG C^[CT]CG[GA]G
BalI 2543 TGGCCA TGG^CCA
BanI 472 GGTGCC G^G[CT][GA]CC
BcgI 1706 GCATTGTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1024 GTATCC GTATCC(6/5)
BfrI 3402 CTTAAG C^TTAAG
BsaHI 4128 GACGTC G[GA]^CG[CT]C
BsaI 4352 GGTCTC GGTCTC(1/5)
BsaWI 2742 ACCGGA [AT]^CCGG[AT]
BseRI 1005 GAGGAG GAGGAG(10/8)
BsoBI 2201 CCCGAG C^[CT]CG[GA]G
BspMI 4118 ACCTGC ACCTGC(4/8)
BsrBI 4160 CCGCTC CCGCTC(-3/-3)
BssSI 1802 CTCGTG CACGAG(-5/-1)
BstUI 4313 CGCG CG^CG
BstXI 2546 CCATGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 4090 CCATGG C^C[GA][CT]GG
Eam1105I 349 GACCAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 814 ATGCAT ATGCA^T
Hin1I 4128 GACGTC G[GA]^CG[CT]C
HpaI 3097 GTTAAC GTT^AAC
MfeI 4803 CAATTG C^AATTG
MluNI 2543 TGGCCA TGG^CCA
MscI 2543 TGGCCA TGG^CCA
MunI 4803 CAATTG C^AATTG
MvnI 4313 CGCG CG^CG
NcoI 4090 CCATGG C^CATGG
NdeI 1334 CATATG CA^TATG
NsiI 814 ATGCAT ATGCA^T
SacI 1100 GAGCTC GAGCT^C
SapI 1532 GAAGAGC GCTCTTC(1/4)
SfcI 4199 CTGTAG C^T[GA][CT]AG
SphI 2749 GCATGC GCATG^C
XbaI 4374 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AocI 1002 CCTGAGG CC^T[ACGT]AGG
4013 CCTGAGG
AseI 3040 ATTAAT AT^TAAT
3518 ATTAAT
AsnI 3040 ATTAAT AT^TAAT
3518 ATTAAT
BclI 1958 TGATCA T^GATCA
3298 TGATCA
BglII 2711 AGATCT A^GATCT
3647 AGATCT
BlpI 1052 GCTTAGC GC^T[ACGT]AGC
2345 GCTCAGC
Bpu1102I 1052 GCTTAGC GC^T[ACGT]AGC
2345 GCTCAGC
BsaAI 253 CACGTA [CT]AC^GT[GA]
1426 CACGTA
BsaBI 1620 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1959 GATCATAATC
Bst1107I 3079 GTATAC GTA^TAC
3151 GTATAC
BstZ17I 3079 GTATAC GTA^TAC
3151 GTATAC
Bsu36I 1002 CCTGAGG CC^T[ACGT]AGG
4013 CCTGAGG
CelII 1052 GCTTAGC GC^T[ACGT]AGC
2345 GCTCAGC
DrdI 597 GACAGCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
691 GACTGTGAAGTC
EaeI 2543 TGGCCA [CT]^GGCC[GA]
4886 TGGCCG
Eco81I 1002 CCTGAGG CC^T[ACGT]AGG
4013 CCTGAGG
FbaI 1958 TGATCA T^GATCA
3298 TGATCA
MamI 1620 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1959 GATCATAATC
MspA1I 2186 CAGCTG C[AC]G^C[GT]G
3912 CAGCTG
PshBI 3040 ATTAAT AT^TAAT
3518 ATTAAT
PvuII 2186 CAGCTG CAG^CTG
3912 CAGCTG
SmlI 3402 CTTAAG C^T[CT][GA]AG
4099 CTCAAG
SspI 1845 AATATT AAT^ATT
2837 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI Aor51HI ApoI Asp700I
AspHI AvaII BamHI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BspHI BsrDI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EarI Eco47III EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI