##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII AccIII AcsI AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AvrII
BbeI BbrPI BbsI BciVI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaWI
BseAI BsiWI BsmBI Bsp1407I BspEI
BsrGI BssHII Bst1107I BstBI BstZ17I
CelII CpoI DraI Eam1105I Eco47III
EcoRI EcoRV EcoT22I FseI HindIII
HpaI KasI KspI MfeI MluI
MroI MunI NarI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
PvuI RsrII SacII SalI SapI
SexAI SfoI SfuI SgrAI SnaBI
SpeI SspBI SspI SwaI XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AclI 4333 AACGTT AA^CGTT
AflIII 3458 ACATGT A^C[GA][CT]GT
AocI 3324 CCTGAGG CC^T[ACGT]AGG
AspI 779 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4523 TGCGCA TGC^GCA
BbvCI 430 CCTCAGC CCTCAGC(-5/-2)
BcgI 3600 GCATTCTTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2438 AGATCT A^GATCT
BsaBI 4089 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 155 CGGCCG CG[GA][CT]^CG
BsmI 3600 GCATTC GAATGC(1/-1)
BspDI 650 ATCGAT AT^CGAT
BspLU11I 3458 ACATGT A^CATGT
BsrDI 3972 GCAATG GCAATG(2/0)
BstEII 1004 GGTGACC G^GT[ACGT]ACC
BstPI 1004 GGTGACC G^GT[ACGT]ACC
Bsu36I 3324 CCTGAGG CC^T[ACGT]AGG
ClaI 650 ATCGAT AT^CGAT
DraIII 3144 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 155 CGGCCG C^GGCCG
EclXI 155 CGGCCG C^GGCCG
Eco52I 155 CGGCCG C^GGCCG
Eco81I 3324 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1004 GGTGACC G^GT[ACGT]ACC
FspI 4523 TGCGCA TGC^GCA
HincII 1493 GTCAAC GT[CT]^[GA]AC
HindII 1493 GTCAAC GT[CT]^[GA]AC
MaeII 4334 ACGT A^CGT
MamI 4089 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI 2557 GCTAGC G^CTAGC
PflFI 779 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3645 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4333 AACGTT AA^CGTT
ScaI 825 AGTACT AGT^ACT
SfiI 296 GGCCGTAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 187 CCCGGG CCC^GGG
TfiI 1975 GATTC G^A[AT]TC
Tth111I 779 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 187 CCCGGG C^CCGGG
XmaI 187 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Acc65I 2618 GGTACC G^GTACC
3364 GGTACC
AccII 137 CGCG CG^CG
713 CGCG
AflII 659 CTTAAG C^TTAAG
3926 CTTAAG
Asp718I 2618 GGTACC G^GTACC
3364 GGTACC
AvaI 19 CTCGGG C^[CT]CG[GA]G
187 CCCGGG
BfrI 659 CTTAAG C^TTAAG
3926 CTTAAG
BsoBI 19 CTCGGG C^[CT]CG[GA]G
187 CCCGGG
BspHI 3126 TCATGA T^CATGA
4348 TCATGA
BstUI 137 CGCG CG^CG
713 CGCG
BstXI 2483 CCATGGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3020 CCAGGGAGCTGG
EcoNI 733 CCTGAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2928 CCTGGTCCAGG
KpnI 2618 GGTACC GGTAC^C
3364 GGTACC
MvnI 137 CGCG CG^CG
713 CGCG
NaeI 153 GCCGGC GCC^GGC
1824 GCCGGC
NgoMIV 153 GCCGGC G^CCGGC
1824 GCCGGC
RcaI 3126 TCATGA T^CATGA
4348 TCATGA
SacI 1786 GAGCTC GAGCT^C
3451 GAGCTC
SbfI 244 CCTGCAGG CCTGCA^GG
3899 CCTGCAGG
Sse8387I 244 CCTGCAGG CCTGCA^GG
3899 CCTGCAGG
StuI 3328 AGGCCT AGG^CCT
4577 AGGCCT
XcmI 1649 CCAACAGTGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3402 CCACGCCAGGACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccI AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaII
BalI BamHI BanI BanII BbvI
BclI BcnI BfaI BglI BmyI
BpmI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DrdI
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII