##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
ApaI AscI Asp700I BbeI BfrI
BlpI Bpu1102I BsaBI BseAI BsiEI
BsiWI BsmBI BspEI BsrFI BssSI
Bst1107I BstZ17I CelII Cfr10I CpoI
EagI EclXI Eco52I FseI KasI
MamI MfeI MluI MroI MunI
NaeI NarI NgoMIV NheI NotI
PaeR7I PinAI PmeI Psp1406I PvuI
RsrII SacI SalI SbfI SexAI
SfiI SfoI SgrAI SphI Sse8387I
StuI SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
Acc65I 4828 GGTACC G^GTACC
AccI 4844 GTCTAC GT^[AC][GT]AC
AhdI 2601 GACTACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3254 GTGCAC G^TGCAC
Aor51HI 3824 AGCGCT AGC^GCT
ApaLI 3254 GTGCAC G^TGCAC
AseI 5409 ATTAAT AT^TAAT
AsnI 5409 ATTAAT AT^TAAT
Asp718I 4828 GGTACC G^GTACC
AspEI 2601 GACTACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1092 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3653 TGCGCA TGC^GCA
AvrII 4060 CCTAGG C^CTAGG
BalI 3315 TGGCCA TGG^CCA
BamHI 1889 GGATCC G^GATCC
BbrPI 2493 CACGTG CAC^GTG
BbvCI 1550 CCTCAGC CCTCAGC(-5/-2)
BclI 3575 TGATCA T^GATCA
BglI 3999 GCCAGATCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4781 AGATCT A^GATCT
BlnI 4060 CCTAGG C^CTAGG
BspDI 3235 ATCGAT AT^CGAT
BssHII 1491 GCGCGC G^CGCGC
BstBI 4067 TTCGAA TT^CGAA
BstEII 4869 GGTCACC G^GT[ACGT]ACC
BstPI 4869 GGTCACC G^GT[ACGT]ACC
ClaI 3235 ATCGAT AT^CGAT
DraIII 2208 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2401 GACCGCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2601 GACTACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3824 AGCGCT AGC^GCT
EcoO65I 4869 GGTCACC G^GT[ACGT]ACC
EcoRV 3880 GATATC GAT^ATC
EcoT22I 3947 ATGCAT ATGCA^T
FbaI 3575 TGATCA T^GATCA
FspI 3653 TGCGCA TGC^GCA
KpnI 4828 GGTACC GGTAC^C
KspI 1367 CCGCGG CCGC^GG
MluNI 3315 TGGCCA TGG^CCA
MscI 3315 TGGCCA TGG^CCA
NdeI 3400 CATATG CA^TATG
NruI 208 TCGCGA TCG^CGA
NsiI 3947 ATGCAT ATGCA^T
NspV 4067 TTCGAA TT^CGAA
PacI 5406 TTAATTAA TTAAT^TAA
PflFI 1092 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1811 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2493 CACGTG CAC^GTG
PmlI 2493 CACGTG CAC^GTG
PshAI 1896 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 5409 ATTAAT AT^TAAT
SacII 1367 CCGCGG CCGC^GG
ScaI 3184 AGTACT AGT^ACT
SfuI 4067 TTCGAA TT^CGAA
SmaI 3512 CCCGGG CCC^GGG
SnaBI 2794 TACGTA TAC^GTA
SspI 5264 AATATT AAT^ATT
Tth111I 1092 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1811 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2248 CCAGGCAAAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 3512 CCCGGG C^CCGGG
XmaI 3512 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AcyI 2559 GGCGTC G[GA]^CG[CT]C
4747 GACGCC
BcgI 991 CGAGCCTCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3276 CGATGACAGTGC
BsaAI 2493 CACGTG [CT]AC^GT[GA]
2794 TACGTA
BsaHI 2559 GGCGTC G[GA]^CG[CT]C
4747 GACGCC
BsaWI 1057 TCCGGT [AT]^CCGG[AT]
4851 TCCGGT
BsmI 1747 GAATGC GAATGC(1/-1)
4594 GAATGC
BspHI 4486 TCATGA T^CATGA
5036 TCATGA
EcoRI 2592 GAATTC G^AATTC
3436 GAATTC
HaeII 2018 AGCGCC [GA]GCGC^[CT]
3824 AGCGCT
Hin1I 2559 GGCGTC G[GA]^CG[CT]C
4747 GACGCC
HincII 4297 GTTAAC GT[CT]^[GA]AC
5227 GTTAAC
HindII 4297 GTTAAC GT[CT]^[GA]AC
5227 GTTAAC
HpaI 4297 GTTAAC GTT^AAC
5227 GTTAAC
RcaI 4486 TCATGA T^CATGA
5036 TCATGA
SpeI 86 ACTAGT A^CTAGT
4933 ACTAGT
XbaI 2956 TCTAGA T^CTAGA
5065 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BciVI BcnI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspLU11I BspMI BsrBI BsrDI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII