##################################
# NO CUT ENZYMES (   61 enzymes) #
##################################

AatII       AccIII      AclI        AgeI        AscI        
AseI        AsnI        AviII       BclI        BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BspDI       
BspEI       BssHII      BstBI       ClaI        CpoI        
EagI        EclXI       Eco52I      EcoRV       EcoT22I     
FbaI        FseI        FspI        KspI        MfeI        
MluI        MroI        MunI        NaeI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PinAI       PmeI        PshBI       
Psp1406I    PvuI        RsrII       SacII       SbfI        
SexAI       SfuI        SgrAI       SmaI        SnaBI       
Sse8387I    SspI        SwaI        XbaI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

Acc65I       2274  GGTACC               G^GTACC
AccII        3028  CGCG                 CG^CG
Aor51HI      2985  AGCGCT               AGC^GCT
Asp718I      2274  GGTACC               G^GTACC
BamHI          61  GGATCC               G^GATCC
BbeI         3254  GGCGCC               GGCGC^C
BbsI           80  GAAGAC               GAAGAC(2/6)
BciVI        3698  GGATAC               GTATCC(6/5)
BglI         1669  GCCTATTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI          80  GAAGAC               GAAGAC(2/6)
BsaBI        2743  GATGCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         4245  GAGACC               GGTCTC(1/5)
BsmI         2946  GCATTC               GAATGC(1/-1)
BsrBI        3107  CCGCTC               CCGCTC(-3/-3)
BsrDI        3660  CATTGC               GCAATG(2/0)
BsrFI        1531  GCCGGT               [GA]^CCGG[CT]
Bst1107I     3286  GTATAC               GTA^TAC
BstUI        3028  CGCG                 CG^CG
BstZ17I      3286  GTATAC               GTA^TAC
Cfr10I       1531  GCCGGT               [GA]^CCGG[CT]
DraIII       1367  CACCGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III     2985  AGCGCT               AGC^GCT
EcoRI        3344  GAATTC               G^AATTC
HgaI          504  GACGC                GACGC(5/10)
HpaI         2630  GTTAAC               GTT^AAC
KasI         3254  GGCGCC               G^GCGCC
KpnI         2274  GGTACC               GGTAC^C
MamI         2743  GATGCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI         3028  CGCG                 CG^CG
NarI         3254  GGCGCC               GG^CGCC
NcoI          201  CCATGG               C^CATGG
PaeR7I       1341  CTCGAG               C^TCGAG
PflMI        3956  CCAGCACTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        1062  GACCCTTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI         1511  GAGCTC               GAGCT^C
SalI          426  GTCGAC               G^TCGAC
SapI         3811  GCTCTTC              GCTCTTC(1/4)
ScaI         3667  AGTACT               AGT^ACT
SfiI         1668  GGCCTATTTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         3254  GGCGCC               GGC^GCC
SphI         4335  GCATGC               GCATG^C
Van91I       3956  CCAGCACTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI           66  CCAAGCAGTTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1341  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   38 enzymes) #
###################################

AcyI          504  GACGCC               G[GA]^CG[CT]C
             3254  GGCGCC              
AhdI         2449  GACCAGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2491  GACTTTTGGTC         
AocI          945  CCTGAGG              CC^T[ACGT]AGG
             2120  CCTGAGG             
Asp700I      3136  GAAGCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3597  GAAGCCATTC          
AspEI        2449  GACCAGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2491  GACTTTTGGTC         
AvrII        3115  CCTAGG               C^CTAGG
             4223  CCTAGG              
BbrPI        2195  CACGTG               CAC^GTG
             4735  CACGTG              
BbvCI        4298  CCTCAGC              CCTCAGC(-5/-2)
             1838  GCTGAGG             
BcgI          503  CGACGCCCATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             3784  GCAGTAAACTCG        
BglII        2700  AGATCT               A^GATCT
             3982  AGATCT              
BlnI         3115  CCTAGG               C^CTAGG
             4223  CCTAGG              
BlpI         2069  GCTCAGC              GC^T[ACGT]AGC
             4236  GCTGAGC             
Bpu1102I     2069  GCTCAGC              GC^T[ACGT]AGC
             4236  GCTGAGC             
BsaHI         504  GACGCC               G[GA]^CG[CT]C
             3254  GGCGCC              
BsgI         1392  CTGCAC               GTGCAG(16/14)
             4732  CTGCAC              
Bsp1407I      785  TGTACA               T^GTACA
             4809  TGTACA              
BspLU11I     2393  ACATGT               A^CATGT
             5024  ACATGT              
BsrGI         785  TGTACA               T^GTACA
             4809  TGTACA              
BssSI         150  CTCGTG               CACGAG(-5/-1)
             3792  CTCGTG              
BstAPI        659  GCAGGCCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3066  GCATGGTCTGC         
BstEII        937  GGTGACC              G^GT[ACGT]ACC
             1273  GGTAACC             
BstPI         937  GGTGACC              G^GT[ACGT]ACC
             1273  GGTAACC             
Bsu36I        945  CCTGAGG              CC^T[ACGT]AGG
             2120  CCTGAGG             
CelII        2069  GCTCAGC              GC^T[ACGT]AGC
             4236  GCTGAGC             
DrdI         2193  GACACGTGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4284  GACGACAATGTC        
Eam1105I     2449  GACCAGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2491  GACTTTTGGTC         
Eco81I        945  CCTGAGG              CC^T[ACGT]AGG
             2120  CCTGAGG             
EcoO65I       937  GGTGACC              G^GT[ACGT]ACC
             1273  GGTAACC             
FauI         1499  CCCGC                CCCGC(4/6)
             1999  GCGGG               
HaeII        2985  AGCGCT               [GA]GCGC^[CT]
             3254  GGCGCC              
Hin1I         504  GACGCC               G[GA]^CG[CT]C
             3254  GGCGCC              
PmaCI        2195  CACGTG               CAC^GTG
             4735  CACGTG              
PmlI         2195  CACGTG               CAC^GTG
             4735  CACGTG              
PpuMI        1042  GGGTCCT              [GA]G^G[AT]CC[CT]
             2871  GGGACCC             
PstI          433  CTGCAG               CTGCA^G
             3240  CTGCAG              
SpeI         1601  ACTAGT               A^CTAGT
             2062  ACTAGT              
SspBI         785  TGTACA               T^GTACA
             4809  TGTACA              
XmnI         3136  GAAGCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3597  GAAGCCATTC          


#########################################
# 3 OR MORE CUT ENZYMES (  115 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AflII       
AflIII      AluI        Alw44I      AlwI        AlwNI       
ApaI        ApaLI       ApoI        AspHI       AspI        
AvaI        AvaII       BalI        BanI        BanII       
BbvI        BcnI        BfaI        BfrI        BmyI        
BpmI        BsaAI       BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspHI       BspMI       BsrI        BssKI       
BstF5I      BstNI       BstXI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraI        
DraII       DsaI        EaeI        EarI        EcoNI       
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HincII      
HindII      HindIII     HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PflFI       PleI        PvuII       
RcaI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       Tth111I     TthHB8I     XhoII