################################## # NO CUT ENZYMES ( 61 enzymes) # ################################## AatII AccIII AclI AgeI AscI AseI AsnI AviII BclI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BssHII BstBI ClaI CpoI EagI EclXI Eco52I EcoRV EcoT22I FbaI FseI FspI KspI MfeI MluI MroI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SbfI SexAI SfuI SgrAI SmaI SnaBI Sse8387I SspI SwaI XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Acc65I 2274 GGTACC G^GTACC AccII 3028 CGCG CG^CG Aor51HI 2985 AGCGCT AGC^GCT Asp718I 2274 GGTACC G^GTACC BamHI 61 GGATCC G^GATCC BbeI 3254 GGCGCC GGCGC^C BbsI 80 GAAGAC GAAGAC(2/6) BciVI 3698 GGATAC GTATCC(6/5) BglI 1669 GCCTATTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 80 GAAGAC GAAGAC(2/6) BsaBI 2743 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 4245 GAGACC GGTCTC(1/5) BsmI 2946 GCATTC GAATGC(1/-1) BsrBI 3107 CCGCTC CCGCTC(-3/-3) BsrDI 3660 CATTGC GCAATG(2/0) BsrFI 1531 GCCGGT [GA]^CCGG[CT] Bst1107I 3286 GTATAC GTA^TAC BstUI 3028 CGCG CG^CG BstZ17I 3286 GTATAC GTA^TAC Cfr10I 1531 GCCGGT [GA]^CCGG[CT] DraIII 1367 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 2985 AGCGCT AGC^GCT EcoRI 3344 GAATTC G^AATTC HgaI 504 GACGC GACGC(5/10) HpaI 2630 GTTAAC GTT^AAC KasI 3254 GGCGCC G^GCGCC KpnI 2274 GGTACC GGTAC^C MamI 2743 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MvnI 3028 CGCG CG^CG NarI 3254 GGCGCC GG^CGCC NcoI 201 CCATGG C^CATGG PaeR7I 1341 CTCGAG C^TCGAG PflMI 3956 CCAGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 1062 GACCCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 1511 GAGCTC GAGCT^C SalI 426 GTCGAC G^TCGAC SapI 3811 GCTCTTC GCTCTTC(1/4) ScaI 3667 AGTACT AGT^ACT SfiI 1668 GGCCTATTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 3254 GGCGCC GGC^GCC SphI 4335 GCATGC GCATG^C Van91I 3956 CCAGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 66 CCAAGCAGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1341 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 38 enzymes) # ################################### AcyI 504 GACGCC G[GA]^CG[CT]C 3254 GGCGCC AhdI 2449 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2491 GACTTTTGGTC AocI 945 CCTGAGG CC^T[ACGT]AGG 2120 CCTGAGG Asp700I 3136 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3597 GAAGCCATTC AspEI 2449 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2491 GACTTTTGGTC AvrII 3115 CCTAGG C^CTAGG 4223 CCTAGG BbrPI 2195 CACGTG CAC^GTG 4735 CACGTG BbvCI 4298 CCTCAGC CCTCAGC(-5/-2) 1838 GCTGAGG BcgI 503 CGACGCCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3784 GCAGTAAACTCG BglII 2700 AGATCT A^GATCT 3982 AGATCT BlnI 3115 CCTAGG C^CTAGG 4223 CCTAGG BlpI 2069 GCTCAGC GC^T[ACGT]AGC 4236 GCTGAGC Bpu1102I 2069 GCTCAGC GC^T[ACGT]AGC 4236 GCTGAGC BsaHI 504 GACGCC G[GA]^CG[CT]C 3254 GGCGCC BsgI 1392 CTGCAC GTGCAG(16/14) 4732 CTGCAC Bsp1407I 785 TGTACA T^GTACA 4809 TGTACA BspLU11I 2393 ACATGT A^CATGT 5024 ACATGT BsrGI 785 TGTACA T^GTACA 4809 TGTACA BssSI 150 CTCGTG CACGAG(-5/-1) 3792 CTCGTG BstAPI 659 GCAGGCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3066 GCATGGTCTGC BstEII 937 GGTGACC G^GT[ACGT]ACC 1273 GGTAACC BstPI 937 GGTGACC G^GT[ACGT]ACC 1273 GGTAACC Bsu36I 945 CCTGAGG CC^T[ACGT]AGG 2120 CCTGAGG CelII 2069 GCTCAGC GC^T[ACGT]AGC 4236 GCTGAGC DrdI 2193 GACACGTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4284 GACGACAATGTC Eam1105I 2449 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2491 GACTTTTGGTC Eco81I 945 CCTGAGG CC^T[ACGT]AGG 2120 CCTGAGG EcoO65I 937 GGTGACC G^GT[ACGT]ACC 1273 GGTAACC FauI 1499 CCCGC CCCGC(4/6) 1999 GCGGG HaeII 2985 AGCGCT [GA]GCGC^[CT] 3254 GGCGCC Hin1I 504 GACGCC G[GA]^CG[CT]C 3254 GGCGCC PmaCI 2195 CACGTG CAC^GTG 4735 CACGTG PmlI 2195 CACGTG CAC^GTG 4735 CACGTG PpuMI 1042 GGGTCCT [GA]G^G[AT]CC[CT] 2871 GGGACCC PstI 433 CTGCAG CTGCA^G 3240 CTGCAG SpeI 1601 ACTAGT A^CTAGT 2062 ACTAGT SspBI 785 TGTACA T^GTACA 4809 TGTACA XmnI 3136 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3597 GAAGCCATTC ######################################### # 3 OR MORE CUT ENZYMES ( 115 enzymes) # ######################################### AccI AciI AcsI AfaI AflII AflIII AluI Alw44I AlwI AlwNI ApaI ApaLI ApoI AspHI AspI AvaI AvaII BalI BanI BanII BbvI BcnI BfaI BfrI BmyI BpmI BsaAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspMI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflFI PleI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XhoII