##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII AccIII AclI AgeI AscI
AseI AsnI AviII BclI BsaWI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BssHII BstBI ClaI CpoI
EagI EclXI Eco52I EcoRV EcoT22I
FbaI FseI FspI KspI MfeI
MluI MroI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PinAI PmeI PshBI
Psp1406I PvuI RsrII SacII SbfI
SexAI SfuI SgrAI SmaI SnaBI
Sse8387I SspI SwaI XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 2274 GGTACC G^GTACC
AccII 3028 CGCG CG^CG
Aor51HI 2985 AGCGCT AGC^GCT
Asp718I 2274 GGTACC G^GTACC
BamHI 61 GGATCC G^GATCC
BbeI 3254 GGCGCC GGCGC^C
BbsI 80 GAAGAC GAAGAC(2/6)
BciVI 3698 GGATAC GTATCC(6/5)
BglI 1669 GCCTATTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 80 GAAGAC GAAGAC(2/6)
BsaBI 2743 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 4245 GAGACC GGTCTC(1/5)
BsmI 2946 GCATTC GAATGC(1/-1)
BsrBI 3107 CCGCTC CCGCTC(-3/-3)
BsrDI 3660 CATTGC GCAATG(2/0)
BsrFI 1531 GCCGGT [GA]^CCGG[CT]
Bst1107I 3286 GTATAC GTA^TAC
BstUI 3028 CGCG CG^CG
BstZ17I 3286 GTATAC GTA^TAC
Cfr10I 1531 GCCGGT [GA]^CCGG[CT]
DraIII 1367 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 2985 AGCGCT AGC^GCT
EcoRI 3344 GAATTC G^AATTC
HgaI 504 GACGC GACGC(5/10)
HpaI 2630 GTTAAC GTT^AAC
KasI 3254 GGCGCC G^GCGCC
KpnI 2274 GGTACC GGTAC^C
MamI 2743 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 3028 CGCG CG^CG
NarI 3254 GGCGCC GG^CGCC
NcoI 201 CCATGG C^CATGG
PaeR7I 1341 CTCGAG C^TCGAG
PflMI 3956 CCAGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1062 GACCCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1511 GAGCTC GAGCT^C
SalI 426 GTCGAC G^TCGAC
SapI 3811 GCTCTTC GCTCTTC(1/4)
ScaI 3667 AGTACT AGT^ACT
SfiI 1668 GGCCTATTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3254 GGCGCC GGC^GCC
SphI 4335 GCATGC GCATG^C
Van91I 3956 CCAGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 66 CCAAGCAGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1341 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AcyI 504 GACGCC G[GA]^CG[CT]C
3254 GGCGCC
AhdI 2449 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2491 GACTTTTGGTC
AocI 945 CCTGAGG CC^T[ACGT]AGG
2120 CCTGAGG
Asp700I 3136 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3597 GAAGCCATTC
AspEI 2449 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2491 GACTTTTGGTC
AvrII 3115 CCTAGG C^CTAGG
4223 CCTAGG
BbrPI 2195 CACGTG CAC^GTG
4735 CACGTG
BbvCI 4298 CCTCAGC CCTCAGC(-5/-2)
1838 GCTGAGG
BcgI 503 CGACGCCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3784 GCAGTAAACTCG
BglII 2700 AGATCT A^GATCT
3982 AGATCT
BlnI 3115 CCTAGG C^CTAGG
4223 CCTAGG
BlpI 2069 GCTCAGC GC^T[ACGT]AGC
4236 GCTGAGC
Bpu1102I 2069 GCTCAGC GC^T[ACGT]AGC
4236 GCTGAGC
BsaHI 504 GACGCC G[GA]^CG[CT]C
3254 GGCGCC
BsgI 1392 CTGCAC GTGCAG(16/14)
4732 CTGCAC
Bsp1407I 785 TGTACA T^GTACA
4809 TGTACA
BspLU11I 2393 ACATGT A^CATGT
5024 ACATGT
BsrGI 785 TGTACA T^GTACA
4809 TGTACA
BssSI 150 CTCGTG CACGAG(-5/-1)
3792 CTCGTG
BstAPI 659 GCAGGCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3066 GCATGGTCTGC
BstEII 937 GGTGACC G^GT[ACGT]ACC
1273 GGTAACC
BstPI 937 GGTGACC G^GT[ACGT]ACC
1273 GGTAACC
Bsu36I 945 CCTGAGG CC^T[ACGT]AGG
2120 CCTGAGG
CelII 2069 GCTCAGC GC^T[ACGT]AGC
4236 GCTGAGC
DrdI 2193 GACACGTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4284 GACGACAATGTC
Eam1105I 2449 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2491 GACTTTTGGTC
Eco81I 945 CCTGAGG CC^T[ACGT]AGG
2120 CCTGAGG
EcoO65I 937 GGTGACC G^GT[ACGT]ACC
1273 GGTAACC
FauI 1499 CCCGC CCCGC(4/6)
1999 GCGGG
HaeII 2985 AGCGCT [GA]GCGC^[CT]
3254 GGCGCC
Hin1I 504 GACGCC G[GA]^CG[CT]C
3254 GGCGCC
PmaCI 2195 CACGTG CAC^GTG
4735 CACGTG
PmlI 2195 CACGTG CAC^GTG
4735 CACGTG
PpuMI 1042 GGGTCCT [GA]G^G[AT]CC[CT]
2871 GGGACCC
PstI 433 CTGCAG CTGCA^G
3240 CTGCAG
SpeI 1601 ACTAGT A^CTAGT
2062 ACTAGT
SspBI 785 TGTACA T^GTACA
4809 TGTACA
XmnI 3136 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3597 GAAGCCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AflIII AluI Alw44I AlwI AlwNI
ApaI ApaLI ApoI AspHI AspI
AvaI AvaII BalI BanI BanII
BbvI BcnI BfaI BfrI BmyI
BpmI BsaAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspHI BspMI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflFI PleI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I XhoII