##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
Aor51HI AscI AspEI AviII BamHI
BclI BfrI BsaBI BsiWI BspDI
BssHII BstBI ClaI DrdI Eam1105I
Eco47III EcoRI EcoRV EcoT22I FbaI
FseI FspI HpaI MamI MfeI
MluI MunI NheI NotI NruI
NsiI NspV PacI PinAI PmeI
Psp1406I PvuI SalI SbfI ScaI
SexAI SfuI SgrAI SnaBI Sse8387I
SwaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 4022 GGTACC G^GTACC
AccIII 1495 TCCGGA T^CCGGA
AseI 4130 ATTAAT AT^TAAT
AsnI 4130 ATTAAT AT^TAAT
Asp700I 3948 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4022 GGTACC G^GTACC
BbeI 2846 GGCGCC GGCGC^C
BbrPI 3014 CACGTG CAC^GTG
BbvCI 374 CCTCAGC CCTCAGC(-5/-2)
BglII 5892 AGATCT A^GATCT
BseAI 1495 TCCGGA T^CCGGA
BsiEI 82 CGGCCG CG[GA][CT]^CG
BsmBI 1202 GAGACG CGTCTC(1/5)
BspEI 1495 TCCGGA T^CCGGA
BstAPI 5452 GCATATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 489 GGTCACC G^GT[ACGT]ACC
BstPI 489 GGTCACC G^GT[ACGT]ACC
CpoI 113 CGGACCG CG^G[AT]CCG
DraIII 5863 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 82 CGGCCG C^GGCCG
EclXI 82 CGGCCG C^GGCCG
Eco52I 82 CGGCCG C^GGCCG
EcoO65I 489 GGTCACC G^GT[ACGT]ACC
HgaI 1204 GACGC GACGC(5/10)
KasI 2846 GGCGCC G^GCGCC
KpnI 4022 GGTACC GGTAC^C
KspI 79 CCGCGG CCGC^GG
MroI 1495 TCCGGA T^CCGGA
NarI 2846 GGCGCC GG^CGCC
NdeI 4344 CATATG CA^TATG
PaeR7I 2137 CTCGAG C^TCGAG
PmaCI 3014 CACGTG CAC^GTG
PmlI 3014 CACGTG CAC^GTG
PshAI 2641 GACAGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4130 ATTAAT AT^TAAT
RsrII 113 CGGACCG CG^G[AT]CCG
SacII 79 CCGCGG CCGC^GG
SfiI 2919 GGCCCCATTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2846 GGCGCC GGC^GCC
SpeI 3625 ACTAGT A^CTAGT
SphI 568 GCATGC GCATG^C
SspI 3743 AATATT AAT^ATT
XbaI 5165 TCTAGA T^CTAGA
XhoI 2137 CTCGAG C^TCGAG
XmnI 3948 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AcyI 1204 GACGCC G[GA]^CG[CT]C
2846 GGCGCC
Alw44I 457 GTGCAC G^TGCAC
639 GTGCAC
AocI 2671 CCTCAGG CC^T[ACGT]AGG
2857 CCTCAGG
ApaLI 457 GTGCAC G^TGCAC
639 GTGCAC
AvrII 3332 CCTAGG C^CTAGG
4069 CCTAGG
BalI 254 TGGCCA TGG^CCA
4997 TGGCCA
BbsI 986 GAAGAC GAAGAC(2/6)
5920 GAAGAC
BlnI 3332 CCTAGG C^CTAGG
4069 CCTAGG
BpuAI 986 GAAGAC GAAGAC(2/6)
5920 GAAGAC
BsaAI 794 CACGTA [CT]AC^GT[GA]
3014 CACGTG
BsaHI 1204 GACGCC G[GA]^CG[CT]C
2846 GGCGCC
BsmI 401 GAATGC GAATGC(1/-1)
4882 GCATTC
Bsp1407I 3817 TGTACA T^GTACA
5545 TGTACA
BspHI 4053 TCATGA T^CATGA
5469 TCATGA
BspLU11I 2535 ACATGT A^CATGT
3458 ACATGT
BspMI 1572 ACCTGC ACCTGC(4/8)
2606 ACCTGC
BsrGI 3817 TGTACA T^GTACA
5545 TGTACA
BssSI 1357 CACGAG CACGAG(-5/-1)
1474 CACGAG
BstYI 1478 AGATCC [GA]^GATC[CT]
5892 AGATCT
Bsu36I 2671 CCTCAGG CC^T[ACGT]AGG
2857 CCTCAGG
DraI 3201 TTTAAA TTT^AAA
3686 TTTAAA
Eco81I 2671 CCTCAGG CC^T[ACGT]AGG
2857 CCTCAGG
HaeII 2846 GGCGCC [GA]GCGC^[CT]
3376 AGCGCC
Hin1I 1204 GACGCC G[GA]^CG[CT]C
2846 GGCGCC
HincII 1378 GTTGAC GT[CT]^[GA]AC
1845 GTCAAC
HindII 1378 GTTGAC GT[CT]^[GA]AC
1845 GTCAAC
MflI 1478 AGATCC [GA]^GATC[CT]
5892 AGATCT
MluNI 254 TGGCCA TGG^CCA
4997 TGGCCA
MscI 254 TGGCCA TGG^CCA
4997 TGGCCA
NcoI 230 CCATGG C^CATGG
4478 CCATGG
PvuII 625 CAGCTG CAG^CTG
2506 CAGCTG
RcaI 4053 TCATGA T^CATGA
5469 TCATGA
SacI 4407 GAGCTC GAGCT^C
5135 GAGCTC
SapI 3832 GCTCTTC GCTCTTC(1/4)
5827 GCTCTTC
SspBI 3817 TGTACA T^GTACA
5545 TGTACA
StuI 1554 AGGCCT AGG^CCT
2424 AGGCCT
XhoII 1478 AGATCC [GA]^GATC[CT]
5892 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApaI
ApoI AspHI AspI AvaI AvaII
BanI BanII BbvI BcgI BciVI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrBI
BsrDI BsrFI BsrI BssKI Bst1107I
BstF5I BstNI BstUI BstXI BstZ17I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XmaCI XmaI