##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AccII AccIII AgeI Aor51HI AscI
AviII BbeI BbrPI BbvCI BsaAI
BsaWI BseAI BsiEI BsiWI Bsp1407I
BspDI BspEI BsrDI BsrGI BssHII
BssSI BstBI BstUI ClaI CpoI
EagI EclXI Eco47III Eco52I EcoRV
EcoT22I FseI FspI HgaI KasI
KspI MfeI MluI MroI MunI
MvnI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflMI PinAI PmaCI PmeI PmlI
PvuI RsrII SacII SalI SapI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SspBI SwaI
Van91I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 2999 GACGTC GACGT^C
AclI 4295 AACGTT AA^CGTT
AcyI 2999 GACGTC G[GA]^CG[CT]C
Alw44I 1703 GTGCAC G^TGCAC
AocI 2721 CCTTAGG CC^T[ACGT]AGG
ApaLI 1703 GTGCAC G^TGCAC
AspI 4779 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 216 CCTAGG C^CTAGG
BalI 3029 TGGCCA TGG^CCA
BciVI 47 GTATCC GTATCC(6/5)
BclI 2316 TGATCA T^GATCA
BcnI 1729 CCCGG CC^[GC]GG
BlnI 216 CCTAGG C^CTAGG
BsaBI 2380 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2999 GACGTC G[GA]^CG[CT]C
BseRI 1251 GAGGAG GAGGAG(10/8)
BsmBI 933 CGTCTC CGTCTC(1/5)
BsrBI 1176 GAGCGG CCGCTC(-3/-3)
BsrFI 730 ACCGGC [GA]^CCGG[CT]
BstEII 3690 GGTGACC G^GT[ACGT]ACC
BstPI 3690 GGTGACC G^GT[ACGT]ACC
Bsu36I 2721 CCTTAGG CC^T[ACGT]AGG
Cfr10I 730 ACCGGC [GA]^CCGG[CT]
DrdI 380 GACTGCTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2721 CCTTAGG CC^T[ACGT]AGG
EcoNI 4799 CCTGGCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3690 GGTGACC G^GT[ACGT]ACC
EcoRI 1675 GAATTC G^AATTC
FbaI 2316 TGATCA T^GATCA
HaeII 553 GGCGCT [GA]GCGC^[CT]
Hin1I 2999 GACGTC G[GA]^CG[CT]C
MamI 2380 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3029 TGGCCA TGG^CCA
MscI 3029 TGGCCA TGG^CCA
MspA1I 3889 CAGCTG C[AC]G^C[GT]G
NciI 1729 CCCGG CC^[GC]GG
NcoI 4338 CCATGG C^CATGG
NdeI 2406 CATATG CA^TATG
PaeR7I 583 CTCGAG C^TCGAG
PflFI 4779 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 799 AGGTCCC [GA]G^G[AT]CC[CT]
Psp1406I 4295 AACGTT AA^CGTT
PvuII 3889 CAGCTG CAG^CTG
SbfI 503 CCTGCAGG CCTGCA^GG
ScaI 2697 AGTACT AGT^ACT
Sse8387I 503 CCTGCAGG CCTGCA^GG
SspI 2174 AATATT AAT^ATT
Tth111I 4779 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 583 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 2794 GGTACC G^GTACC
3744 GGTACC
AflII 3207 CTTAAG C^TTAAG
3281 CTTAAG
AflIII 481 ACATGT A^C[GA][CT]GT
4717 ACATGT
AhdI 631 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
793 GACAGCAGGTC
ApaI 96 GGGCCC GGGCC^C
1181 GGGCCC
AseI 256 ATTAAT AT^TAAT
3997 ATTAAT
AsnI 256 ATTAAT AT^TAAT
3997 ATTAAT
Asp718I 2794 GGTACC G^GTACC
3744 GGTACC
AspEI 631 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
793 GACAGCAGGTC
AvaII 800 GGTCC G^G[AT]CC
4778 GGACC
BamHI 221 GGATCC G^GATCC
854 GGATCC
BcgI 4788 CGAGTGTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1072 GCAGAGTAGTCG
BfrI 3207 CTTAAG C^TTAAG
3281 CTTAAG
BglI 1809 GCCTTTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4611 GCCTGGCAGGC
BglII 655 AGATCT A^GATCT
4331 AGATCT
BlpI 4424 GCTCAGC GC^T[ACGT]AGC
4625 GCTAAGC
Bpu1102I 4424 GCTCAGC GC^T[ACGT]AGC
4625 GCTAAGC
BsmI 914 GCATTC GAATGC(1/-1)
1818 GCATTC
BspLU11I 481 ACATGT A^CATGT
4717 ACATGT
Bst1107I 2107 GTATAC GTA^TAC
2580 GTATAC
BstAPI 2884 GCAGAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4735 GCAGTCCATGC
BstXI 835 CCAGGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4283 CCATTTTACTGG
BstZ17I 2107 GTATAC GTA^TAC
2580 GTATAC
CelII 4424 GCTCAGC GC^T[ACGT]AGC
4625 GCTAAGC
DraI 3158 TTTAAA TTT^AAA
4084 TTTAAA
DraIII 1706 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
3354 CACACAGTG
DsaI 1185 CCACGG C^C[GA][CT]GG
4338 CCATGG
EaeI 2037 TGGCCG [CT]^GGCC[GA]
3029 TGGCCA
Eam1105I 631 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
793 GACAGCAGGTC
FauI 3626 CCCGC CCCGC(4/6)
1178 GCGGG
HapII 731 CCGG C^CGG
1730 CCGG
HpaI 4486 GTTAAC GTT^AAC
4509 GTTAAC
HpaII 731 CCGG C^CGG
1730 CCGG
KpnI 2794 GGTACC GGTAC^C
3744 GGTACC
MspI 731 CCGG C^CGG
1730 CCGG
PshAI 4154 GACTTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4460 GACATCTGTC
PshBI 256 ATTAAT AT^TAAT
3997 ATTAAT
SphI 2071 GCATGC GCATG^C
4520 GCATGC
StuI 205 AGGCCT AGG^CCT
1720 AGGCCT
XbaI 764 TCTAGA T^CTAGA
2537 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI BanI BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspHI
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HhaI
HinP1I HincII HindII HindIII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PstI
RcaI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmnI