##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII AccIII AclI AcyI AhdI
Aor51HI ApaI AscI AseI AsnI
AspEI BbeI BbrPI BbvCI BcnI
BglI BglII BpmI BsaHI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BspHI BssHII BssSI Bst1107I BstEII
BstPI BstZ17I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I FseI HaeII Hin1I
HpaI KasI KspI MluI MroI
NaeI NarI NciI NdeI NgoMIV
NotI NruI PacI PaeR7I PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacII SfiI SfoI
SgrAI SmaI SwaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 178 GGTACC G^GTACC
AccII 2462 CGCG CG^CG
AgeI 1312 ACCGGT A^CCGGT
Alw44I 3007 GTGCAC G^TGCAC
AocI 2114 CCTCAGG CC^T[ACGT]AGG
ApaLI 3007 GTGCAC G^TGCAC
Asp700I 1396 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 178 GGTACC G^GTACC
AspI 1978 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 5178 CTCGGG C^[CT]CG[GA]G
AviII 596 TGCGCA TGC^GCA
AvrII 2178 CCTAGG C^CTAGG
BamHI 2675 GGATCC G^GATCC
BcgI 1811 GCAAGAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2232 GGATAC GTATCC(6/5)
BlnI 2178 CCTAGG C^CTAGG
BsaWI 1312 ACCGGT [AT]^CCGG[AT]
BsoBI 5178 CTCGGG C^[CT]CG[GA]G
BsrBI 2434 CCGCTC CCGCTC(-3/-3)
BstBI 1505 TTCGAA TT^CGAA
BstUI 2462 CGCG CG^CG
BstXI 2240 CCAACACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 2675 GGATCC [GA]^GATC[CT]
Bsu36I 2114 CCTCAGG CC^T[ACGT]AGG
CfoI 597 GCGC GCG^C
DraII 5094 AGGTCCT [GA]G^G[ACGT]CC[CT]
Eco81I 2114 CCTCAGG CC^T[ACGT]AGG
EcoO109I 5094 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 3683 GAATTC G^AATTC
EcoRV 4645 GATATC GAT^ATC
FspI 596 TGCGCA TGC^GCA
HhaI 597 GCGC GCG^C
HinP1I 597 GCGC G^CGC
KpnI 178 GGTACC GGTAC^C
MflI 2675 GGATCC [GA]^GATC[CT]
MvnI 2462 CGCG CG^CG
NheI 4628 GCTAGC G^CTAGC
NspV 1505 TTCGAA TT^CGAA
PflFI 1978 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1312 ACCGGT A^CCGGT
PpuMI 5094 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 4001 GACTTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 4838 GAGCTC GAGCT^C
SalI 1288 GTCGAC G^TCGAC
SfuI 1505 TTCGAA TT^CGAA
SnaBI 5384 TACGTA TAC^GTA
StuI 317 AGGCCT AGG^CCT
Tth111I 1978 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2004 TCTAGA T^CTAGA
XhoII 2675 GGATCC [GA]^GATC[CT]
XmnI 1396 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflII 1023 CTTAAG C^TTAAG
5938 CTTAAG
BanII 4838 GAGCTC G[GA]GC[CT]^C
5746 GGGCTC
BfrI 1023 CTTAAG C^TTAAG
5938 CTTAAG
BsaAI 3523 CACGTA [CT]AC^GT[GA]
5384 TACGTA
BsaBI 97 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1936 GATGTTGATC
BsaI 1270 GAGACC GGTCTC(1/5)
1847 GAGACC
BseRI 4505 CTCCTC GAGGAG(10/8)
5632 GAGGAG
BsmFI 796 GGGAC GGGAC(10/14)
4008 GTCCC
BsmI 1737 GAATGC GAATGC(1/-1)
2494 GCATTC
BspLU11I 1518 ACATGT A^CATGT
3764 ACATGT
DraIII 381 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
2372 CACAGAGTG
EcoT22I 2150 ATGCAT ATGCA^T
6173 ATGCAT
HgaI 5442 GACGC GACGC(5/10)
1084 GCGTC
HindIII 4247 AAGCTT A^AGCTT
4929 AAGCTT
MamI 97 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1936 GATGTTGATC
NsiI 2150 ATGCAT ATGCA^T
6173 ATGCAT
SapI 3967 GAAGAGC GCTCTTC(1/4)
3659 GCTCTTC
SbfI 2210 CCTGCAGG CCTGCA^GG
5329 CCTGCAGG
SexAI 590 ACCTGGT A^CC[AT]GGT
956 ACCAGGT
SphI 765 GCATGC GCATG^C
3880 GCATGC
Sse8387I 2210 CCTGCAGG CCTGCA^GG
5329 CCTGCAGG
XcmI 866 CCAATCCTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2842 CCAATGAAAACATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaII BalI BanI BbsI BbvI
BclI BfaI BlpI BmyI Bpu1102I
BpuAI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI Bsp1286I Bsp1407I BspMI
BsrDI BsrFI BsrGI BsrI BssKI
BstAPI BstF5I BstNI Cac8I CelII
Cfr10I Cfr13I DdeI DpnII DraI
DsaI EaeI EarI EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MfeI MluNI
MnlI MscI MseI MslI MspA1I
MspI MunI MvaI MwoI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SpeI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I