################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## AatII Acc65I AccII AccIII AcyI AgeI AhdI AocI Aor51HI ApaI AscI Asp700I Asp718I AspEI AviII BbeI BclI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BsrBI BsrDI BsrFI BssHII Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I FbaI FseI FspI HaeII HgaI Hin1I HpaI KasI KpnI KspI MamI MluI MroI MvnI NaeI NarI NgoMIV NotI NruI NspV PinAI PmeI PvuI RsrII SacII SalI SbfI SexAI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SwaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AclI 3063 AACGTT AA^CGTT AflII 5754 CTTAAG C^TTAAG AlwNI 3818 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG AspI 4218 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 5368 CCTAGG C^CTAGG BamHI 1688 GGATCC G^GATCC BanI 5561 GGTGCC G^G[CT][GA]CC BbrPI 149 CACGTG CAC^GTG BbvCI 4382 CCTCAGC CCTCAGC(-5/-2) BcgI 5889 GCAGACCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 3273 GTATCC GTATCC(6/5) BfrI 5754 CTTAAG C^TTAAG BglI 5663 GCCAGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 5104 AGATCT A^GATCT BlnI 5368 CCTAGG C^CTAGG Bsp1407I 937 TGTACA T^GTACA BsrGI 937 TGTACA T^GTACA BssSI 669 CACGAG CACGAG(-5/-1) BstXI 3548 CCAGAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 740 GCGC GCG^C EcoNI 2485 CCTTTATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 4829 ATGCAT ATGCA^T HhaI 740 GCGC GCG^C HinP1I 740 GCGC G^CGC MfeI 1438 CAATTG C^AATTG MunI 1438 CAATTG C^AATTG NdeI 3585 CATATG CA^TATG NheI 4347 GCTAGC G^CTAGC NsiI 4829 ATGCAT ATGCA^T PacI 6296 TTAATTAA TTAAT^TAA PaeR7I 5897 CTCGAG C^TCGAG PflFI 4218 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 149 CACGTG CAC^GTG PmlI 149 CACGTG CAC^GTG PshAI 3860 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 3063 AACGTT AA^CGTT SfiI 5662 GGCCAGGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 1876 CCCGGG CCC^GGG SphI 619 GCATGC GCATG^C SspBI 937 TGTACA T^GTACA StuI 3628 AGGCCT AGG^CCT Tth111I 4218 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 3664 CCACTGGCCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 5897 CTCGAG C^TCGAG XmaCI 1876 CCCGGG C^CCGGG XmaI 1876 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### Alw44I 115 GTGCAC G^TGCAC 2316 GTGCAC ApaLI 115 GTGCAC G^TGCAC 2316 GTGCAC AvaI 1876 CCCGGG C^[CT]CG[GA]G 5897 CTCGAG BbsI 2339 GAAGAC GAAGAC(2/6) 1003 GTCTTC BlpI 24 GCTGAGC GC^T[ACGT]AGC 4398 GCTTAGC Bpu1102I 24 GCTGAGC GC^T[ACGT]AGC 4398 GCTTAGC BpuAI 2339 GAAGAC GAAGAC(2/6) 1003 GTCTTC BsaAI 149 CACGTG [CT]AC^GT[GA] 4502 TACGTG BsoBI 1876 CCCGGG C^[CT]CG[GA]G 5897 CTCGAG BspHI 190 TCATGA T^CATGA 2937 TCATGA BspMI 4929 ACCTGC ACCTGC(4/8) 1118 GCAGGT BstEII 3070 GGTTACC G^GT[ACGT]ACC 4925 GGTAACC BstPI 3070 GGTTACC G^GT[ACGT]ACC 4925 GGTAACC CelII 24 GCTGAGC GC^T[ACGT]AGC 4398 GCTTAGC EcoO65I 3070 GGTTACC G^GT[ACGT]ACC 4925 GGTAACC EcoRI 80 GAATTC G^AATTC 431 GAATTC EcoRV 3078 GATATC GAT^ATC 3887 GATATC FauI 5482 CCCGC CCCGC(4/6) 883 GCGGG HapII 782 CCGG C^CGG 1877 CCGG HpaII 782 CCGG C^CGG 1877 CCGG MspI 782 CCGG C^CGG 1877 CCGG NcoI 743 CCATGG C^CATGG 3929 CCATGG PpuMI 504 AGGTCCT [GA]G^G[AT]CC[CT] 3806 AGGACCC PstI 590 CTGCAG CTGCA^G 642 CTGCAG RcaI 190 TCATGA T^CATGA 2937 TCATGA SapI 29 GCTCTTC GCTCTTC(1/4) 185 GCTCTTC ScaI 942 AGTACT AGT^ACT 3988 AGTACT XbaI 200 TCTAGA T^CTAGA 1249 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 102 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI ApoI AseI AsnI AspHI AvaII BalI BanII BbvI BcnI BfaI BmyI BpmI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspLU11I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HincII HindII HindIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PshBI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII