##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI AhdI AocI Aor51HI ApaI
AscI Asp700I Asp718I AspEI AviII
BbeI BclI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BsrBI BsrDI BsrFI BssHII
Bst1107I BstAPI BstBI BstUI BstZ17I
Bsu36I Cfr10I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I FbaI FseI FspI HaeII
HgaI Hin1I HpaI KasI KpnI
KspI MamI MluI MroI MvnI
NaeI NarI NgoMIV NotI NruI
NspV PinAI PmeI PvuI RsrII
SacII SalI SbfI SexAI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AclI 3063 AACGTT AA^CGTT
AflII 5754 CTTAAG C^TTAAG
AlwNI 3818 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 4218 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 5368 CCTAGG C^CTAGG
BamHI 1688 GGATCC G^GATCC
BanI 5561 GGTGCC G^G[CT][GA]CC
BbrPI 149 CACGTG CAC^GTG
BbvCI 4382 CCTCAGC CCTCAGC(-5/-2)
BcgI 5889 GCAGACCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3273 GTATCC GTATCC(6/5)
BfrI 5754 CTTAAG C^TTAAG
BglI 5663 GCCAGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 5104 AGATCT A^GATCT
BlnI 5368 CCTAGG C^CTAGG
Bsp1407I 937 TGTACA T^GTACA
BsrGI 937 TGTACA T^GTACA
BssSI 669 CACGAG CACGAG(-5/-1)
BstXI 3548 CCAGAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 740 GCGC GCG^C
EcoNI 2485 CCTTTATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 4829 ATGCAT ATGCA^T
HhaI 740 GCGC GCG^C
HinP1I 740 GCGC G^CGC
MfeI 1438 CAATTG C^AATTG
MunI 1438 CAATTG C^AATTG
NdeI 3585 CATATG CA^TATG
NheI 4347 GCTAGC G^CTAGC
NsiI 4829 ATGCAT ATGCA^T
PacI 6296 TTAATTAA TTAAT^TAA
PaeR7I 5897 CTCGAG C^TCGAG
PflFI 4218 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 149 CACGTG CAC^GTG
PmlI 149 CACGTG CAC^GTG
PshAI 3860 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3063 AACGTT AA^CGTT
SfiI 5662 GGCCAGGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1876 CCCGGG CCC^GGG
SphI 619 GCATGC GCATG^C
SspBI 937 TGTACA T^GTACA
StuI 3628 AGGCCT AGG^CCT
Tth111I 4218 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 3664 CCACTGGCCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 5897 CTCGAG C^TCGAG
XmaCI 1876 CCCGGG C^CCGGG
XmaI 1876 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Alw44I 115 GTGCAC G^TGCAC
2316 GTGCAC
ApaLI 115 GTGCAC G^TGCAC
2316 GTGCAC
AvaI 1876 CCCGGG C^[CT]CG[GA]G
5897 CTCGAG
BbsI 2339 GAAGAC GAAGAC(2/6)
1003 GTCTTC
BlpI 24 GCTGAGC GC^T[ACGT]AGC
4398 GCTTAGC
Bpu1102I 24 GCTGAGC GC^T[ACGT]AGC
4398 GCTTAGC
BpuAI 2339 GAAGAC GAAGAC(2/6)
1003 GTCTTC
BsaAI 149 CACGTG [CT]AC^GT[GA]
4502 TACGTG
BsoBI 1876 CCCGGG C^[CT]CG[GA]G
5897 CTCGAG
BspHI 190 TCATGA T^CATGA
2937 TCATGA
BspMI 4929 ACCTGC ACCTGC(4/8)
1118 GCAGGT
BstEII 3070 GGTTACC G^GT[ACGT]ACC
4925 GGTAACC
BstPI 3070 GGTTACC G^GT[ACGT]ACC
4925 GGTAACC
CelII 24 GCTGAGC GC^T[ACGT]AGC
4398 GCTTAGC
EcoO65I 3070 GGTTACC G^GT[ACGT]ACC
4925 GGTAACC
EcoRI 80 GAATTC G^AATTC
431 GAATTC
EcoRV 3078 GATATC GAT^ATC
3887 GATATC
FauI 5482 CCCGC CCCGC(4/6)
883 GCGGG
HapII 782 CCGG C^CGG
1877 CCGG
HpaII 782 CCGG C^CGG
1877 CCGG
MspI 782 CCGG C^CGG
1877 CCGG
NcoI 743 CCATGG C^CATGG
3929 CCATGG
PpuMI 504 AGGTCCT [GA]G^G[AT]CC[CT]
3806 AGGACCC
PstI 590 CTGCAG CTGCA^G
642 CTGCAG
RcaI 190 TCATGA T^CATGA
2937 TCATGA
SapI 29 GCTCTTC GCTCTTC(1/4)
185 GCTCTTC
ScaI 942 AGTACT AGT^ACT
3988 AGTACT
XbaI 200 TCTAGA T^CTAGA
1249 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI AseI AsnI
AspHI AvaII BalI BanII BbvI
BcnI BfaI BmyI BpmI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspLU11I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HincII HindII HindIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PshBI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII