##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII AclI AgeI AhdI Aor51HI
AscI AspEI AspI AvrII BbeI
BbrPI BcgI BlnI BsiEI BsiWI
BspDI BssHII BssSI BstBI ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FseI
HaeII HpaI KasI KspI MfeI
MunI NarI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacII SalI SfiI SfoI SfuI
SgrAI SnaBI SpeI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccII 4321 CGCG CG^CG
AccIII 243 TCCGGA T^CCGGA
AcyI 2063 GGCGTC G[GA]^CG[CT]C
AflII 3188 CTTAAG C^TTAAG
AseI 5206 ATTAAT AT^TAAT
AsnI 5206 ATTAAT AT^TAAT
Asp700I 4960 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 2193 GGATCC G^GATCC
BciVI 6602 GTATCC GTATCC(6/5)
BclI 1112 TGATCA T^GATCA
BfrI 3188 CTTAAG C^TTAAG
BsaAI 2670 TACGTG [CT]AC^GT[GA]
BsaBI 4494 GATATAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2063 GGCGTC G[GA]^CG[CT]C
BsaWI 243 TCCGGA [AT]^CCGG[AT]
BseAI 243 TCCGGA T^CCGGA
BsmBI 1008 CGTCTC CGTCTC(1/5)
BspEI 243 TCCGGA T^CCGGA
Bst1107I 1348 GTATAC GTA^TAC
BstEII 4799 GGTCACC G^GT[ACGT]ACC
BstPI 4799 GGTCACC G^GT[ACGT]ACC
BstUI 4321 CGCG CG^CG
BstZ17I 1348 GTATAC GTA^TAC
EcoO65I 4799 GGTCACC G^GT[ACGT]ACC
FbaI 1112 TGATCA T^GATCA
Hin1I 2063 GGCGTC G[GA]^CG[CT]C
MamI 4494 GATATAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 4320 ACGCGT A^CGCGT
MroI 243 TCCGGA T^CCGGA
MvnI 4321 CGCG CG^CG
NaeI 1048 GCCGGC GCC^GGC
NgoMIV 1048 GCCGGC G^CCGGC
PaeR7I 804 CTCGAG C^TCGAG
PshAI 5171 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 5206 ATTAAT AT^TAAT
SapI 7117 GCTCTTC GCTCTTC(1/4)
SbfI 3783 CCTGCAGG CCTGCA^GG
ScaI 624 AGTACT AGT^ACT
SexAI 6895 ACCAGGT A^CC[AT]GGT
SmaI 3497 CCCGGG CCC^GGG
Sse8387I 3783 CCTGCAGG CCTGCA^GG
SspI 5570 AATATT AAT^ATT
XbaI 4325 TCTAGA T^CTAGA
XhoI 804 CTCGAG C^TCGAG
XmaCI 3497 CCCGGG C^CCGGG
XmaI 3497 CCCGGG C^CCGGG
XmnI 4960 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Acc65I 3511 GGTACC G^GTACC
4093 GGTACC
ApaI 1003 GGGCCC GGGCC^C
6712 GGGCCC
Asp718I 3511 GGTACC G^GTACC
4093 GGTACC
BcnI 3497 CCCGG CC^[GC]GG
3498 CCGGG
BglI 1043 GCCCTGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
6493 GCCTCTCTGGC
BglII 1526 AGATCT A^GATCT
3806 AGATCT
BsmI 1983 GAATGC GAATGC(1/-1)
1619 GCATTC
Bsp1407I 3755 TGTACA T^GTACA
5247 TGTACA
BsrBI 683 GAGCGG CCGCTC(-3/-3)
1026 GAGCGG
BsrDI 5116 CATTGC GCAATG(2/0)
3083 GCAATG
BsrFI 1048 GCCGGC [GA]^CCGG[CT]
2981 GCCGGT
BsrGI 3755 TGTACA T^GTACA
5247 TGTACA
BstAPI 701 GCACAGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
6388 GCAGGAGCTGC
Cfr10I 1048 GCCGGC [GA]^CCGG[CT]
2981 GCCGGT
FauI 698 CCCGC CCCGC(4/6)
481 GCGGG
HincII 2771 GTCAAC GT[CT]^[GA]AC
6085 GTCAAC
HindII 2771 GTCAAC GT[CT]^[GA]AC
6085 GTCAAC
HindIII 2327 AAGCTT A^AGCTT
3005 AAGCTT
KpnI 3511 GGTACC GGTAC^C
4093 GGTACC
MaeII 2671 ACGT A^CGT
5997 ACGT
NciI 3497 CCCGG CC^[GC]GG
3498 CCGGG
NdeI 1376 CATATG CA^TATG
4001 CATATG
NheI 1973 GCTAGC G^CTAGC
4304 GCTAGC
SacI 1487 GAGCTC GAGCT^C
5788 GAGCTC
SspBI 3755 TGTACA T^GTACA
5247 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI AocI
ApaLI ApoI AspHI AvaI AvaII
AviII BalI BanI BanII BbsI
BbvCI BbvI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspHI
BspLU11I BspMI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DraIII DrdI DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI FokI FspI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII