################################## # NO CUT ENZYMES ( 60 enzymes) # ################################## AatII AclI AgeI AhdI Aor51HI AscI AspEI AspI AvrII BbeI BbrPI BcgI BlnI BsiEI BsiWI BspDI BssHII BssSI BstBI ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FseI HaeII HpaI KasI KspI MfeI MunI NarI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SalI SfiI SfoI SfuI SgrAI SnaBI SpeI SwaI Tth111I ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccII 4321 CGCG CG^CG AccIII 243 TCCGGA T^CCGGA AcyI 2063 GGCGTC G[GA]^CG[CT]C AflII 3188 CTTAAG C^TTAAG AseI 5206 ATTAAT AT^TAAT AsnI 5206 ATTAAT AT^TAAT Asp700I 4960 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 2193 GGATCC G^GATCC BciVI 6602 GTATCC GTATCC(6/5) BclI 1112 TGATCA T^GATCA BfrI 3188 CTTAAG C^TTAAG BsaAI 2670 TACGTG [CT]AC^GT[GA] BsaBI 4494 GATATAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 2063 GGCGTC G[GA]^CG[CT]C BsaWI 243 TCCGGA [AT]^CCGG[AT] BseAI 243 TCCGGA T^CCGGA BsmBI 1008 CGTCTC CGTCTC(1/5) BspEI 243 TCCGGA T^CCGGA Bst1107I 1348 GTATAC GTA^TAC BstEII 4799 GGTCACC G^GT[ACGT]ACC BstPI 4799 GGTCACC G^GT[ACGT]ACC BstUI 4321 CGCG CG^CG BstZ17I 1348 GTATAC GTA^TAC EcoO65I 4799 GGTCACC G^GT[ACGT]ACC FbaI 1112 TGATCA T^GATCA Hin1I 2063 GGCGTC G[GA]^CG[CT]C MamI 4494 GATATAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 4320 ACGCGT A^CGCGT MroI 243 TCCGGA T^CCGGA MvnI 4321 CGCG CG^CG NaeI 1048 GCCGGC GCC^GGC NgoMIV 1048 GCCGGC G^CCGGC PaeR7I 804 CTCGAG C^TCGAG PshAI 5171 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 5206 ATTAAT AT^TAAT SapI 7117 GCTCTTC GCTCTTC(1/4) SbfI 3783 CCTGCAGG CCTGCA^GG ScaI 624 AGTACT AGT^ACT SexAI 6895 ACCAGGT A^CC[AT]GGT SmaI 3497 CCCGGG CCC^GGG Sse8387I 3783 CCTGCAGG CCTGCA^GG SspI 5570 AATATT AAT^ATT XbaI 4325 TCTAGA T^CTAGA XhoI 804 CTCGAG C^TCGAG XmaCI 3497 CCCGGG C^CCGGG XmaI 3497 CCCGGG C^CCGGG XmnI 4960 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### Acc65I 3511 GGTACC G^GTACC 4093 GGTACC ApaI 1003 GGGCCC GGGCC^C 6712 GGGCCC Asp718I 3511 GGTACC G^GTACC 4093 GGTACC BcnI 3497 CCCGG CC^[GC]GG 3498 CCGGG BglI 1043 GCCCTGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 6493 GCCTCTCTGGC BglII 1526 AGATCT A^GATCT 3806 AGATCT BsmI 1983 GAATGC GAATGC(1/-1) 1619 GCATTC Bsp1407I 3755 TGTACA T^GTACA 5247 TGTACA BsrBI 683 GAGCGG CCGCTC(-3/-3) 1026 GAGCGG BsrDI 5116 CATTGC GCAATG(2/0) 3083 GCAATG BsrFI 1048 GCCGGC [GA]^CCGG[CT] 2981 GCCGGT BsrGI 3755 TGTACA T^GTACA 5247 TGTACA BstAPI 701 GCACAGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 6388 GCAGGAGCTGC Cfr10I 1048 GCCGGC [GA]^CCGG[CT] 2981 GCCGGT FauI 698 CCCGC CCCGC(4/6) 481 GCGGG HincII 2771 GTCAAC GT[CT]^[GA]AC 6085 GTCAAC HindII 2771 GTCAAC GT[CT]^[GA]AC 6085 GTCAAC HindIII 2327 AAGCTT A^AGCTT 3005 AAGCTT KpnI 3511 GGTACC GGTAC^C 4093 GGTACC MaeII 2671 ACGT A^CGT 5997 ACGT NciI 3497 CCCGG CC^[GC]GG 3498 CCGGG NdeI 1376 CATATG CA^TATG 4001 CATATG NheI 1973 GCTAGC G^CTAGC 4304 GCTAGC SacI 1487 GAGCTC GAGCT^C 5788 GAGCTC SspBI 3755 TGTACA T^GTACA 5247 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 126 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI Alw44I AlwI AlwNI AocI ApaLI ApoI AspHI AvaI AvaII AviII BalI BanI BanII BbsI BbvCI BbvI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspLU11I BspMI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DraIII DrdI DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI FspI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII