################################## # NO CUT ENZYMES ( 79 enzymes) # ################################## AatII Acc65I AccII AccIII AhdI Aor51HI AscI AseI AsnI Asp718I AspEI AspI BalI BbeI BbrPI BbvCI BsaAI BsaBI BseAI BsiWI Bsp1407I BspDI BspEI BspHI BsrGI BssHII Bst1107I BstAPI BstBI BstUI BstZ17I ClaI CpoI DrdI Eam1105I Eco47III FseI HincII HindII HpaI KasI KpnI KspI MamI MluI MluNI MroI MscI MvnI NaeI NarI NdeI NgoMIV NheI NruI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PshBI PvuI RcaI RsrII SacII SalI SbfI SfoI SfuI SgrAI SnaBI Sse8387I SspBI SspI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AclI 1226 AACGTT AA^CGTT AcyI 537 GGCGTC G[GA]^CG[CT]C AflII 2768 CTTAAG C^TTAAG AflIII 4203 ACATGT A^C[GA][CT]GT AgeI 713 ACCGGT A^CCGGT Alw44I 3672 GTGCAC G^TGCAC ApaI 2142 GGGCCC GGGCC^C ApaLI 3672 GTGCAC G^TGCAC Asp700I 562 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 4816 TGCGCA TGC^GCA AvrII 4100 CCTAGG C^CTAGG BcgI 1617 GCAGAAACGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2014 GGATAC GTATCC(6/5) BclI 483 TGATCA T^GATCA BfrI 2768 CTTAAG C^TTAAG BlnI 4100 CCTAGG C^CTAGG BlpI 2 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2 GCTGAGC GC^T[ACGT]AGC BsaHI 537 GGCGTC G[GA]^CG[CT]C BsaWI 713 ACCGGT [AT]^CCGG[AT] BsgI 5038 GTGCAG GTGCAG(16/14) BspLU11I 4203 ACATGT A^CATGT BsrFI 713 ACCGGT [GA]^CCGG[CT] BssSI 352 CTCGTG CACGAG(-5/-1) CelII 2 GCTGAGC GC^T[ACGT]AGC Cfr10I 713 ACCGGT [GA]^CCGG[CT] DraIII 4494 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 42 CGGCCG C^GGCCG EclXI 42 CGGCCG C^GGCCG Eco52I 42 CGGCCG C^GGCCG EcoNI 2823 CCTCTGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1183 GAATTC G^AATTC EcoRV 2842 GATATC GAT^ATC FbaI 483 TGATCA T^GATCA FspI 4816 TGCGCA TGC^GCA HaeII 1808 AGCGCC [GA]GCGC^[CT] Hin1I 537 GGCGTC G[GA]^CG[CT]C MfeI 5492 CAATTG C^AATTG MunI 5492 CAATTG C^AATTG NotI 41 GCGGCCGC GC^GGCCGC PinAI 713 ACCGGT A^CCGGT Psp1406I 1226 AACGTT AA^CGTT SexAI 527 ACCTGGT A^CC[AT]GGT SfiI 1761 GGCCGTGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SpeI 3544 ACTAGT A^CTAGT XmnI 562 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### BamHI 2284 GGATCC G^GATCC 4573 GGATCC BglI 84 GCCCTTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1762 GCCGTGAAGGC BsiEI 42 CGGCCG CG[GA][CT]^CG 754 CGGTCG BsmBI 337 GAGACG CGTCTC(1/5) 2729 GAGACG BspMI 1469 ACCTGC ACCTGC(4/8) 231 GCAGGT BsrBI 29 CCGCTC CCGCTC(-3/-3) 862 GAGCGG BstEII 445 GGTGACC G^GT[ACGT]ACC 3131 GGTCACC BstPI 445 GGTGACC G^GT[ACGT]ACC 3131 GGTCACC BstXI 4508 CCACCCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5019 CCATGACCATGG DraI 3043 TTTAAA TTT^AAA 4774 TTTAAA EaeI 42 CGGCCG [CT]^GGCC[GA] 2062 CGGCCA EcoO65I 445 GGTGACC G^GT[ACGT]ACC 3131 GGTCACC EcoT22I 2573 ATGCAT ATGCA^T 3648 ATGCAT NsiI 2573 ATGCAT ATGCA^T 3648 ATGCAT PpuMI 1157 AGGACCC [GA]G^G[AT]CC[CT] 1466 AGGACCT PshAI 3077 GACCTAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 4233 GACACTTGTC PvuII 1216 CAGCTG CAG^CTG 4878 CAGCTG SapI 2403 GAAGAGC GCTCTTC(1/4) 5071 GCTCTTC ScaI 2233 AGTACT AGT^ACT 5467 AGTACT StuI 2735 AGGCCT AGG^CCT 4730 AGGCCT XbaI 4659 TCTAGA T^CTAGA 4760 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 112 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BglII BmyI BpmI BpuAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SphI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI