##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccII AccIII AhdI
Aor51HI AscI AseI AsnI Asp718I
AspEI AspI BalI BbeI BbrPI
BbvCI BsaAI BsaBI BseAI BsiWI
Bsp1407I BspDI BspEI BspHI BsrGI
BssHII Bst1107I BstAPI BstBI BstUI
BstZ17I ClaI CpoI DrdI Eam1105I
Eco47III FseI HincII HindII HpaI
KasI KpnI KspI MamI MluI
MluNI MroI MscI MvnI NaeI
NarI NdeI NgoMIV NheI NruI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PshBI PvuI RcaI
RsrII SacII SalI SbfI SfoI
SfuI SgrAI SnaBI Sse8387I SspBI
SspI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AclI 1226 AACGTT AA^CGTT
AcyI 537 GGCGTC G[GA]^CG[CT]C
AflII 2768 CTTAAG C^TTAAG
AflIII 4203 ACATGT A^C[GA][CT]GT
AgeI 713 ACCGGT A^CCGGT
Alw44I 3672 GTGCAC G^TGCAC
ApaI 2142 GGGCCC GGGCC^C
ApaLI 3672 GTGCAC G^TGCAC
Asp700I 562 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 4816 TGCGCA TGC^GCA
AvrII 4100 CCTAGG C^CTAGG
BcgI 1617 GCAGAAACGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2014 GGATAC GTATCC(6/5)
BclI 483 TGATCA T^GATCA
BfrI 2768 CTTAAG C^TTAAG
BlnI 4100 CCTAGG C^CTAGG
BlpI 2 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2 GCTGAGC GC^T[ACGT]AGC
BsaHI 537 GGCGTC G[GA]^CG[CT]C
BsaWI 713 ACCGGT [AT]^CCGG[AT]
BsgI 5038 GTGCAG GTGCAG(16/14)
BspLU11I 4203 ACATGT A^CATGT
BsrFI 713 ACCGGT [GA]^CCGG[CT]
BssSI 352 CTCGTG CACGAG(-5/-1)
CelII 2 GCTGAGC GC^T[ACGT]AGC
Cfr10I 713 ACCGGT [GA]^CCGG[CT]
DraIII 4494 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 42 CGGCCG C^GGCCG
EclXI 42 CGGCCG C^GGCCG
Eco52I 42 CGGCCG C^GGCCG
EcoNI 2823 CCTCTGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1183 GAATTC G^AATTC
EcoRV 2842 GATATC GAT^ATC
FbaI 483 TGATCA T^GATCA
FspI 4816 TGCGCA TGC^GCA
HaeII 1808 AGCGCC [GA]GCGC^[CT]
Hin1I 537 GGCGTC G[GA]^CG[CT]C
MfeI 5492 CAATTG C^AATTG
MunI 5492 CAATTG C^AATTG
NotI 41 GCGGCCGC GC^GGCCGC
PinAI 713 ACCGGT A^CCGGT
Psp1406I 1226 AACGTT AA^CGTT
SexAI 527 ACCTGGT A^CC[AT]GGT
SfiI 1761 GGCCGTGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 3544 ACTAGT A^CTAGT
XmnI 562 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BamHI 2284 GGATCC G^GATCC
4573 GGATCC
BglI 84 GCCCTTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1762 GCCGTGAAGGC
BsiEI 42 CGGCCG CG[GA][CT]^CG
754 CGGTCG
BsmBI 337 GAGACG CGTCTC(1/5)
2729 GAGACG
BspMI 1469 ACCTGC ACCTGC(4/8)
231 GCAGGT
BsrBI 29 CCGCTC CCGCTC(-3/-3)
862 GAGCGG
BstEII 445 GGTGACC G^GT[ACGT]ACC
3131 GGTCACC
BstPI 445 GGTGACC G^GT[ACGT]ACC
3131 GGTCACC
BstXI 4508 CCACCCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5019 CCATGACCATGG
DraI 3043 TTTAAA TTT^AAA
4774 TTTAAA
EaeI 42 CGGCCG [CT]^GGCC[GA]
2062 CGGCCA
EcoO65I 445 GGTGACC G^GT[ACGT]ACC
3131 GGTCACC
EcoT22I 2573 ATGCAT ATGCA^T
3648 ATGCAT
NsiI 2573 ATGCAT ATGCA^T
3648 ATGCAT
PpuMI 1157 AGGACCC [GA]G^G[AT]CC[CT]
1466 AGGACCT
PshAI 3077 GACCTAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4233 GACACTTGTC
PvuII 1216 CAGCTG CAG^CTG
4878 CAGCTG
SapI 2403 GAAGAGC GCTCTTC(1/4)
5071 GCTCTTC
ScaI 2233 AGTACT AGT^ACT
5467 AGTACT
StuI 2735 AGGCCT AGG^CCT
4730 AGGCCT
XbaI 4659 TCTAGA T^CTAGA
4760 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BglII BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PstI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI SphI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI