##################################
# NO CUT ENZYMES (   79 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AhdI        
Aor51HI     AscI        AseI        AsnI        Asp718I     
AspEI       AspI        BalI        BbeI        BbrPI       
BbvCI       BsaAI       BsaBI       BseAI       BsiWI       
Bsp1407I    BspDI       BspEI       BspHI       BsrGI       
BssHII      Bst1107I    BstAPI      BstBI       BstUI       
BstZ17I     ClaI        CpoI        DrdI        Eam1105I    
Eco47III    FseI        HincII      HindII      HpaI        
KasI        KpnI        KspI        MamI        MluI        
MluNI       MroI        MscI        MvnI        NaeI        
NarI        NdeI        NgoMIV      NheI        NruI        
NspV        PacI        PaeR7I      PflFI       PmaCI       
PmeI        PmlI        PshBI       PvuI        RcaI        
RsrII       SacII       SalI        SbfI        SfoI        
SfuI        SgrAI       SnaBI       Sse8387I    SspBI       
SspI        SwaI        Tth111I     XhoI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AclI         1226  AACGTT               AA^CGTT
AcyI          537  GGCGTC               G[GA]^CG[CT]C
AflII        2768  CTTAAG               C^TTAAG
AflIII       4203  ACATGT               A^C[GA][CT]GT
AgeI          713  ACCGGT               A^CCGGT
Alw44I       3672  GTGCAC               G^TGCAC
ApaI         2142  GGGCCC               GGGCC^C
ApaLI        3672  GTGCAC               G^TGCAC
Asp700I       562  GAAGAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII        4816  TGCGCA               TGC^GCA
AvrII        4100  CCTAGG               C^CTAGG
BcgI         1617  GCAGAAACGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        2014  GGATAC               GTATCC(6/5)
BclI          483  TGATCA               T^GATCA
BfrI         2768  CTTAAG               C^TTAAG
BlnI         4100  CCTAGG               C^CTAGG
BlpI            2  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I        2  GCTGAGC              GC^T[ACGT]AGC
BsaHI         537  GGCGTC               G[GA]^CG[CT]C
BsaWI         713  ACCGGT               [AT]^CCGG[AT]
BsgI         5038  GTGCAG               GTGCAG(16/14)
BspLU11I     4203  ACATGT               A^CATGT
BsrFI         713  ACCGGT               [GA]^CCGG[CT]
BssSI         352  CTCGTG               CACGAG(-5/-1)
CelII           2  GCTGAGC              GC^T[ACGT]AGC
Cfr10I        713  ACCGGT               [GA]^CCGG[CT]
DraIII       4494  CACCAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI           42  CGGCCG               C^GGCCG
EclXI          42  CGGCCG               C^GGCCG
Eco52I         42  CGGCCG               C^GGCCG
EcoNI        2823  CCTCTGTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1183  GAATTC               G^AATTC
EcoRV        2842  GATATC               GAT^ATC
FbaI          483  TGATCA               T^GATCA
FspI         4816  TGCGCA               TGC^GCA
HaeII        1808  AGCGCC               [GA]GCGC^[CT]
Hin1I         537  GGCGTC               G[GA]^CG[CT]C
MfeI         5492  CAATTG               C^AATTG
MunI         5492  CAATTG               C^AATTG
NotI           41  GCGGCCGC             GC^GGCCGC
PinAI         713  ACCGGT               A^CCGGT
Psp1406I     1226  AACGTT               AA^CGTT
SexAI         527  ACCTGGT              A^CC[AT]GGT
SfiI         1761  GGCCGTGAAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI         3544  ACTAGT               A^CTAGT
XmnI          562  GAAGAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

BamHI        2284  GGATCC               G^GATCC
             4573  GGATCC              
BglI           84  GCCCTTGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1762  GCCGTGAAGGC         
BsiEI          42  CGGCCG               CG[GA][CT]^CG
              754  CGGTCG              
BsmBI         337  GAGACG               CGTCTC(1/5)
             2729  GAGACG              
BspMI        1469  ACCTGC               ACCTGC(4/8)
              231  GCAGGT              
BsrBI          29  CCGCTC               CCGCTC(-3/-3)
              862  GAGCGG              
BstEII        445  GGTGACC              G^GT[ACGT]ACC
             3131  GGTCACC             
BstPI         445  GGTGACC              G^GT[ACGT]ACC
             3131  GGTCACC             
BstXI        4508  CCACCCATGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             5019  CCATGACCATGG        
DraI         3043  TTTAAA               TTT^AAA
             4774  TTTAAA              
EaeI           42  CGGCCG               [CT]^GGCC[GA]
             2062  CGGCCA              
EcoO65I       445  GGTGACC              G^GT[ACGT]ACC
             3131  GGTCACC             
EcoT22I      2573  ATGCAT               ATGCA^T
             3648  ATGCAT              
NsiI         2573  ATGCAT               ATGCA^T
             3648  ATGCAT              
PpuMI        1157  AGGACCC              [GA]G^G[AT]CC[CT]
             1466  AGGACCT             
PshAI        3077  GACCTAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             4233  GACACTTGTC          
PvuII        1216  CAGCTG               CAG^CTG
             4878  CAGCTG              
SapI         2403  GAAGAGC              GCTCTTC(1/4)
             5071  GCTCTTC             
ScaI         2233  AGTACT               AGT^ACT
             5467  AGTACT              
StuI         2735  AGGCCT               AGG^CCT
             4730  AGGCCT              
XbaI         4659  TCTAGA               T^CTAGA
             4760  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (  112 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       AocI        ApoI        AspHI       
AvaI        AvaII       BanI        BanII       BbsI        
BbvI        BcnI        BfaI        BglII       BmyI        
BpmI        BpuAI       BsaI        BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        BsoBI       Bsp1286I    BsrDI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Bsu36I      
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EarI        Eco81I      EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        HinP1I      
HindIII     HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PstI        RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmaI        SmlI        SphI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XhoII       
XmaCI       XmaI