##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AccII AflII AgeI AhdI AocI
Aor51HI AscI AspEI AviII AvrII
BbeI BbrPI BbvCI BciVI BclI
BcnI BfrI BlnI BsiEI BsiWI
BspHI BsrBI BsrFI BssHII BssSI
BstEII BstPI BstUI Bsu36I Cfr10I
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoT22I FauI FbaI FseI FspI
KasI KspI MluI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI PacI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfoI SgrAI
SmaI SnaBI SphI Sse8387I SwaI
XbaI XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 1099 GACGTC GACGT^C
Acc65I 550 GGTACC G^GTACC
AccI 2868 GTATAC GT^[AC][GT]AC
AccIII 1754 TCCGGA T^CCGGA
AclI 2357 AACGTT AA^CGTT
Asp718I 550 GGTACC G^GTACC
AspI 1624 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2124 GGATCC G^GATCC
BcgI 2493 GCACGATCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1581 AGATCT A^GATCT
BsaAI 1597 TACGTG [CT]AC^GT[GA]
BseAI 1754 TCCGGA T^CCGGA
BsmBI 2449 GAGACG CGTCTC(1/5)
BsmI 1497 GAATGC GAATGC(1/-1)
Bsp1407I 989 TGTACA T^GTACA
BspDI 2081 ATCGAT AT^CGAT
BspEI 1754 TCCGGA T^CCGGA
BsrGI 989 TGTACA T^GTACA
Bst1107I 2868 GTATAC GTA^TAC
BstBI 3299 TTCGAA TT^CGAA
BstZ17I 2868 GTATAC GTA^TAC
CfoI 3995 GCGC GCG^C
ClaI 2081 ATCGAT AT^CGAT
DraI 2983 TTTAAA TTT^AAA
DraII 2636 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 2636 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRI 4518 GAATTC G^AATTC
HaeII 3994 GGCGCT [GA]GCGC^[CT]
HhaI 3995 GCGC GCG^C
HinP1I 3995 GCGC G^CGC
HpaI 1785 GTTAAC GTT^AAC
KpnI 550 GGTACC GGTAC^C
MroI 1754 TCCGGA T^CCGGA
NspV 3299 TTCGAA TT^CGAA
PaeR7I 2501 CTCGAG C^TCGAG
PflFI 1624 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 2357 AACGTT AA^CGTT
SapI 1867 GAAGAGC GCTCTTC(1/4)
SfiI 2638 GGCCCACATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 3299 TTCGAA TT^CGAA
SpeI 1555 ACTAGT A^CTAGT
SspBI 989 TGTACA T^GTACA
StuI 2657 AGGCCT AGG^CCT
Tth111I 1624 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2501 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 1099 GACGTC G[GA]^CG[CT]C
2719 GACGCC
Alw44I 256 GTGCAC G^TGCAC
534 GTGCAC
ApaLI 256 GTGCAC G^TGCAC
534 GTGCAC
AseI 556 ATTAAT AT^TAAT
1588 ATTAAT
AsnI 556 ATTAAT AT^TAAT
1588 ATTAAT
Asp700I 1965 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4259 GAATTTATTC
AvaI 440 CTCGGG C^[CT]CG[GA]G
2501 CTCGAG
BglI 280 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2639 GCCCACATGGC
BlpI 1936 GCTGAGC GC^T[ACGT]AGC
2311 GCTCAGC
Bpu1102I 1936 GCTGAGC GC^T[ACGT]AGC
2311 GCTCAGC
BsaHI 1099 GACGTC G[GA]^CG[CT]C
2719 GACGCC
BsaWI 1754 TCCGGA [AT]^CCGG[AT]
2802 ACCGGA
BseRI 447 CTCCTC GAGGAG(10/8)
1318 CTCCTC
BsoBI 440 CTCGGG C^[CT]CG[GA]G
2501 CTCGAG
BspLU11I 2875 ACATGT A^CATGT
4117 ACATGT
BstXI 2209 CCAACAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3378 CCATGGAGGTGG
CelII 1936 GCTGAGC GC^T[ACGT]AGC
2311 GCTCAGC
DsaI 996 CCATGG C^C[GA][CT]GG
3378 CCATGG
EcoRV 1758 GATATC GAT^ATC
3631 GATATC
EcoT14I 996 CCATGG C^C[AT][AT]GG
3378 CCATGG
HapII 1755 CCGG C^CGG
2803 CCGG
Hin1I 1099 GACGTC G[GA]^CG[CT]C
2719 GACGCC
HindIII 460 AAGCTT A^AGCTT
4388 AAGCTT
HpaII 1755 CCGG C^CGG
2803 CCGG
MspI 1755 CCGG C^CGG
2803 CCGG
NcoI 996 CCATGG C^CATGG
3378 CCATGG
NspI 2875 ACATGT [GA]CATG^[CT]
4117 ACATGT
PshBI 556 ATTAAT AT^TAAT
1588 ATTAAT
PvuII 1119 CAGCTG CAG^CTG
2339 CAGCTG
SacI 1330 GAGCTC GAGCT^C
1926 GAGCTC
SspI 2443 AATATT AAT^ATT
3469 AATATT
StyI 996 CCATGG C^C[AT][AT]GG
3378 CCATGG
XmnI 1965 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4259 GAATTTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApaI ApoI AspHI
AvaII BalI BanI BanII BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaBI BsaI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII EaeI EarI EcoNI
EcoRII Fnu4HI FokI HaeIII HgaI
HincII HindII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MfeI MflI MluNI
MnlI MscI MseI MslI MspA1I
MunI MvaI MwoI NdeII NlaIII
NlaIV PflMI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII