##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
Aor51HI AscI Asp700I AvrII BamHI
BbeI BbrPI BciVI BfrI BlnI
BsaAI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstZ17I ClaI CpoI EagI EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
FseI HpaI KasI KspI MaeII
MluI MroI NarI NotI PacI
PaeR7I PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacII SapI
SbfI SexAI SfiI SfoI SgrAI
SnaBI SpeI Sse8387I SwaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 180 GGTACC G^GTACC
AccI 2816 GTCGAC GT^[AC][GT]AC
AccII 73 CGCG CG^CG
AcyI 3805 GGCGTC G[GA]^CG[CT]C
AflIII 105 ACATGT A^C[GA][CT]GT
AhdI 4291 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 207 ATTAAT AT^TAAT
AsnI 207 ATTAAT AT^TAAT
Asp718I 180 GGTACC G^GTACC
AspEI 4291 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2485 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1655 TGCGCA TGC^GCA
BbvCI 2080 GCTGAGG CCTCAGC(-5/-2)
BcgI 3126 GCAACATGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 493 GCCCGAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 3805 GGCGTC G[GA]^CG[CT]C
Bsp1407I 3421 TGTACA T^GTACA
BspLU11I 105 ACATGT A^CATGT
BsrFI 2032 GCCGGC [GA]^CCGG[CT]
BsrGI 3421 TGTACA T^GTACA
BstBI 4085 TTCGAA TT^CGAA
BstUI 73 CGCG CG^CG
Cfr10I 2032 GCCGGC [GA]^CCGG[CT]
DrdI 266 GACATCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4291 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 3 GCGGG CCCGC(4/6)
FspI 1655 TGCGCA TGC^GCA
HaeII 3219 AGCGCC [GA]GCGC^[CT]
Hin1I 3805 GGCGTC G[GA]^CG[CT]C
HindIII 1861 AAGCTT A^AGCTT
KpnI 180 GGTACC GGTAC^C
MvnI 73 CGCG CG^CG
NaeI 2032 GCCGGC GCC^GGC
NgoMIV 2032 GCCGGC G^CCGGC
NheI 4538 GCTAGC G^CTAGC
NruI 72 TCGCGA TCG^CGA
NspV 4085 TTCGAA TT^CGAA
PflFI 2485 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 4757 GACATTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 207 ATTAAT AT^TAAT
SalI 2816 GTCGAC G^TCGAC
ScaI 1555 AGTACT AGT^ACT
SfuI 4085 TTCGAA TT^CGAA
SmaI 3392 CCCGGG CCC^GGG
SspBI 3421 TGTACA T^GTACA
SspI 1611 AATATT AAT^ATT
StuI 2373 AGGCCT AGG^CCT
Tth111I 2485 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 3392 CCCGGG C^CCGGG
XmaI 3392 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Alw44I 1589 GTGCAC G^TGCAC
4155 GTGCAC
AocI 859 CCTGAGG CC^T[ACGT]AGG
4739 CCTGAGG
ApaLI 1589 GTGCAC G^TGCAC
4155 GTGCAC
AvaI 494 CCCGAG C^[CT]CG[GA]G
3392 CCCGGG
BclI 504 TGATCA T^GATCA
1550 TGATCA
BglII 797 AGATCT A^GATCT
3735 AGATCT
BsoBI 494 CCCGAG C^[CT]CG[GA]G
3392 CCCGGG
Bsu36I 859 CCTGAGG CC^T[ACGT]AGG
4739 CCTGAGG
DraI 387 TTTAAA TTT^AAA
1569 TTTAAA
DraIII 1262 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
4227 CACAGTGTG
Eco81I 859 CCTGAGG CC^T[ACGT]AGG
4739 CCTGAGG
EcoNI 859 CCTGAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4547 CCTTCTAGAGG
EcoT14I 159 CCATGG C^C[AT][AT]GG
3024 CCATGG
EcoT22I 356 ATGCAT ATGCA^T
1378 ATGCAT
FbaI 504 TGATCA T^GATCA
1550 TGATCA
HincII 2816 GTCGAC GT[CT]^[GA]AC
3717 GTTGAC
HindII 2816 GTCGAC GT[CT]^[GA]AC
3717 GTTGAC
NcoI 159 CCATGG C^CATGG
3024 CCATGG
NsiI 356 ATGCAT ATGCA^T
1378 ATGCAT
SphI 1376 GCATGC GCATG^C
1762 GCATGC
StyI 159 CCATGG C^C[AT][AT]GG
3024 CCATGG
XbaI 784 TCTAGA T^CTAGA
4550 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApaI ApoI AspHI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaBI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MamI
MboI MboII MfeI MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MunI MvaI MwoI NciI
NdeI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RcaI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII