##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
Acc65I AccII AclI AflII AgeI
Aor51HI AscI AseI AsnI Asp718I
AspI AviII AvrII BcgI BclI
BfrI BlnI BsaBI BsiEI BsiWI
BsmBI BspDI BspLU11I BssHII Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
ClaI CpoI EagI EclXI Eco47III
Eco52I EcoO65I EcoRV FbaI FseI
FspI HpaI KpnI KspI MamI
MluI MvnI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SwaI Tth111I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccIII 3674 TCCGGA T^CCGGA
AflIII 648 ACACGT A^C[GA][CT]GT
AhdI 1582 GACAAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2649 GTGCAC G^TGCAC
ApaLI 2649 GTGCAC G^TGCAC
Asp700I 3573 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1582 GACAAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 205 GGATCC G^GATCC
BbeI 1146 GGCGCC GGCGC^C
BbrPI 649 CACGTG CAC^GTG
BlpI 853 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 853 GCTGAGC GC^T[ACGT]AGC
BsaWI 3674 TCCGGA [AT]^CCGG[AT]
BseAI 3674 TCCGGA T^CCGGA
BspEI 3674 TCCGGA T^CCGGA
BssSI 4075 CTCGTG CACGAG(-5/-1)
BstAPI 1433 GCAGCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 853 GCTGAGC GC^T[ACGT]AGC
Eam1105I 1582 GACAAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 4883 ATGCAT ATGCA^T
HindIII 2203 AAGCTT A^AGCTT
KasI 1146 GGCGCC G^GCGCC
MroI 3674 TCCGGA T^CCGGA
NarI 1146 GGCGCC GG^CGCC
NheI 2011 GCTAGC G^CTAGC
NsiI 4883 ATGCAT ATGCA^T
PmaCI 649 CACGTG CAC^GTG
PmlI 649 CACGTG CAC^GTG
SapI 1227 GAAGAGC GCTCTTC(1/4)
SfoI 1146 GGCGCC GGC^GCC
SpeI 3 ACTAGT A^CTAGT
SspI 604 AATATT AAT^ATT
XmnI 3573 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AatII 1247 GACGTC GACGT^C
3693 GACGTC
AccI 223 GTCTAC GT^[AC][GT]AC
5109 GTAGAC
ApaI 1287 GGGCCC GGGCC^C
2663 GGGCCC
BalI 1752 TGGCCA TGG^CCA
2367 TGGCCA
BbvCI 1107 CCTCAGC CCTCAGC(-5/-2)
3874 CCTCAGC
BcnI 677 CCGGG CC^[GC]GG
894 CCCGG
BsaAI 649 CACGTG [CT]AC^GT[GA]
3724 TACGTG
BsmI 4298 GAATGC GAATGC(1/-1)
960 GCATTC
Bsp1407I 4108 TGTACA T^GTACA
4487 TGTACA
BspMI 3906 ACCTGC ACCTGC(4/8)
2278 GCAGGT
BsrBI 3926 CCGCTC CCGCTC(-3/-3)
4032 CCGCTC
BsrGI 4108 TGTACA T^GTACA
4487 TGTACA
DraIII 4632 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
5005 CACATTGTG
DrdI 1510 GACTCGGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1906 GACATCCCAGTC
EcoRI 64 GAATTC G^AATTC
4477 GAATTC
HaeII 1146 GGCGCC [GA]GCGC^[CT]
1375 GGCGCT
HgaI 3532 GACGC GACGC(5/10)
4607 GCGTC
HincII 637 GTCAAC GT[CT]^[GA]AC
3157 GTCAAC
HindII 637 GTCAAC GT[CT]^[GA]AC
3157 GTCAAC
MfeI 3124 CAATTG C^AATTG
5379 CAATTG
MluNI 1752 TGGCCA TGG^CCA
2367 TGGCCA
MscI 1752 TGGCCA TGG^CCA
2367 TGGCCA
MunI 3124 CAATTG C^AATTG
5379 CAATTG
NaeI 2640 GCCGGC GCC^GGC
2801 GCCGGC
NciI 677 CCGGG CC^[GC]GG
894 CCCGG
NcoI 2353 CCATGG C^CATGG
4564 CCATGG
NdeI 3167 CATATG CA^TATG
4282 CATATG
NgoMIV 2640 GCCGGC G^CCGGC
2801 GCCGGC
NspI 4528 ACATGC [GA]CATG^[CT]
4881 ACATGC
SspBI 4108 TGTACA T^GTACA
4487 TGTACA
StuI 1100 AGGCCT AGG^CCT
2372 AGGCCT
TaqI 2564 TCGA T^CGA
3181 TCGA
TthHB8I 2564 TCGA T^CGA
3181 TCGA
XcmI 1617 CCAGGACCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2891 CCAGCCAGTATGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AvaI AvaII BanI BanII BbsI
BbvI BciVI BfaI BglI BglII
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspHI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RcaI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XhoII