################################## # NO CUT ENZYMES ( 78 enzymes) # ################################## Acc65I AccII AclI AflII AgeI Aor51HI AscI AseI AsnI Asp718I AspI AviII AvrII BcgI BclI BfrI BlnI BsaBI BsiEI BsiWI BsmBI BspDI BspLU11I BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I ClaI CpoI EagI EclXI Eco47III Eco52I EcoO65I EcoRV FbaI FseI FspI HpaI KpnI KspI MamI MluI MvnI NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SphI Sse8387I SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AccIII 3674 TCCGGA T^CCGGA AflIII 648 ACACGT A^C[GA][CT]GT AhdI 1582 GACAAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2649 GTGCAC G^TGCAC ApaLI 2649 GTGCAC G^TGCAC Asp700I 3573 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1582 GACAAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 205 GGATCC G^GATCC BbeI 1146 GGCGCC GGCGC^C BbrPI 649 CACGTG CAC^GTG BlpI 853 GCTGAGC GC^T[ACGT]AGC Bpu1102I 853 GCTGAGC GC^T[ACGT]AGC BsaWI 3674 TCCGGA [AT]^CCGG[AT] BseAI 3674 TCCGGA T^CCGGA BspEI 3674 TCCGGA T^CCGGA BssSI 4075 CTCGTG CACGAG(-5/-1) BstAPI 1433 GCAGCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 853 GCTGAGC GC^T[ACGT]AGC Eam1105I 1582 GACAAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT22I 4883 ATGCAT ATGCA^T HindIII 2203 AAGCTT A^AGCTT KasI 1146 GGCGCC G^GCGCC MroI 3674 TCCGGA T^CCGGA NarI 1146 GGCGCC GG^CGCC NheI 2011 GCTAGC G^CTAGC NsiI 4883 ATGCAT ATGCA^T PmaCI 649 CACGTG CAC^GTG PmlI 649 CACGTG CAC^GTG SapI 1227 GAAGAGC GCTCTTC(1/4) SfoI 1146 GGCGCC GGC^GCC SpeI 3 ACTAGT A^CTAGT SspI 604 AATATT AAT^ATT XmnI 3573 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AatII 1247 GACGTC GACGT^C 3693 GACGTC AccI 223 GTCTAC GT^[AC][GT]AC 5109 GTAGAC ApaI 1287 GGGCCC GGGCC^C 2663 GGGCCC BalI 1752 TGGCCA TGG^CCA 2367 TGGCCA BbvCI 1107 CCTCAGC CCTCAGC(-5/-2) 3874 CCTCAGC BcnI 677 CCGGG CC^[GC]GG 894 CCCGG BsaAI 649 CACGTG [CT]AC^GT[GA] 3724 TACGTG BsmI 4298 GAATGC GAATGC(1/-1) 960 GCATTC Bsp1407I 4108 TGTACA T^GTACA 4487 TGTACA BspMI 3906 ACCTGC ACCTGC(4/8) 2278 GCAGGT BsrBI 3926 CCGCTC CCGCTC(-3/-3) 4032 CCGCTC BsrGI 4108 TGTACA T^GTACA 4487 TGTACA DraIII 4632 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG 5005 CACATTGTG DrdI 1510 GACTCGGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1906 GACATCCCAGTC EcoRI 64 GAATTC G^AATTC 4477 GAATTC HaeII 1146 GGCGCC [GA]GCGC^[CT] 1375 GGCGCT HgaI 3532 GACGC GACGC(5/10) 4607 GCGTC HincII 637 GTCAAC GT[CT]^[GA]AC 3157 GTCAAC HindII 637 GTCAAC GT[CT]^[GA]AC 3157 GTCAAC MfeI 3124 CAATTG C^AATTG 5379 CAATTG MluNI 1752 TGGCCA TGG^CCA 2367 TGGCCA MscI 1752 TGGCCA TGG^CCA 2367 TGGCCA MunI 3124 CAATTG C^AATTG 5379 CAATTG NaeI 2640 GCCGGC GCC^GGC 2801 GCCGGC NciI 677 CCGGG CC^[GC]GG 894 CCCGG NcoI 2353 CCATGG C^CATGG 4564 CCATGG NdeI 3167 CATATG CA^TATG 4282 CATATG NgoMIV 2640 GCCGGC G^CCGGC 2801 GCCGGC NspI 4528 ACATGC [GA]CATG^[CT] 4881 ACATGC SspBI 4108 TGTACA T^GTACA 4487 TGTACA StuI 1100 AGGCCT AGG^CCT 2372 AGGCCT TaqI 2564 TCGA T^CGA 3181 TCGA TthHB8I 2564 TCGA T^CGA 3181 TCGA XcmI 1617 CCAGGACCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2891 CCAGCCAGTATGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 113 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvI BciVI BfaI BglI BglII BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XhoII