##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
AatII AclI AflII AgeI AscI
AvrII BamHI BcgI BfrI BlnI
BsiEI BsiWI BspDI BssHII Bst1107I
BstBI BstZ17I ClaI CpoI DraI
EagI EclXI Eco52I FseI HindIII
MfeI MluI MunI NheI NotI
NruI NspV PacI PaeR7I PinAI
PmeI Psp1406I PvuI RsrII SalI
SapI ScaI SfiI SfuI SmaI
SnaBI SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AcsI 4853 GAATTC [GA]^AATT[CT]
Aor51HI 3125 AGCGCT AGC^GCT
ApoI 4853 GAATTC [GA]^AATT[CT]
AseI 3727 ATTAAT AT^TAAT
AsnI 3727 ATTAAT AT^TAAT
Asp700I 3421 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 2372 GGCGCC GGCGC^C
BclI 4336 TGATCA T^GATCA
BsaBI 5760 GATAATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 5143 TCATGA T^CATGA
BspLU11I 3158 ACATGT A^CATGT
BsrFI 2375 GCCGGC [GA]^CCGG[CT]
BssSI 4407 CTCGTG CACGAG(-5/-1)
Cfr10I 2375 GCCGGC [GA]^CCGG[CT]
DraIII 923 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 3125 AGCGCT AGC^GCT
EcoRI 4853 GAATTC G^AATTC
EcoRV 244 GATATC GAT^ATC
FbaI 4336 TGATCA T^GATCA
HpaI 1225 GTTAAC GTT^AAC
KasI 2372 GGCGCC G^GCGCC
KspI 5064 CCGCGG CCGC^GG
MamI 5760 GATAATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 2375 GCCGGC GCC^GGC
NarI 2372 GGCGCC GG^CGCC
NgoMIV 2375 GCCGGC G^CCGGC
PflMI 5293 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 6158 GACCTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3727 ATTAAT AT^TAAT
RcaI 5143 TCATGA T^CATGA
SacII 5064 CCGCGG CCGC^GG
SbfI 1362 CCTGCAGG CCTGCA^GG
SexAI 5045 ACCAGGT A^CC[AT]GGT
SfoI 2372 GGCGCC GGC^GCC
SgrAI 2374 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 6305 ACTAGT A^CTAGT
SphI 1823 GCATGC GCATG^C
Sse8387I 1362 CCTGCAGG CCTGCA^GG
Van91I 5293 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 5684 TCTAGA T^CTAGA
XmnI 3421 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Acc65I 544 GGTACC G^GTACC
5987 GGTACC
AccI 1753 GTCTAC GT^[AC][GT]AC
5426 GTCTAC
AccII 1927 CGCG CG^CG
5065 CGCG
AhdI 2599 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3026 GACTGCTGGTC
AocI 2569 CCTGAGG CC^T[ACGT]AGG
6135 CCTCAGG
Asp718I 544 GGTACC G^GTACC
5987 GGTACC
AspEI 2599 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3026 GACTGCTGGTC
BbrPI 871 CACGTG CAC^GTG
2335 CACGTG
BglII 1763 AGATCT A^GATCT
3394 AGATCT
BsmI 616 GAATGC GAATGC(1/-1)
3646 GAATGC
Bsp1407I 3161 TGTACA T^GTACA
6243 TGTACA
BsrGI 3161 TGTACA T^GTACA
6243 TGTACA
BstUI 1927 CGCG CG^CG
5065 CGCG
Bsu36I 2569 CCTGAGG CC^T[ACGT]AGG
6135 CCTCAGG
Eam1105I 2599 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3026 GACTGCTGGTC
Eco81I 2569 CCTGAGG CC^T[ACGT]AGG
6135 CCTCAGG
EcoT22I 2086 ATGCAT ATGCA^T
4900 ATGCAT
KpnI 544 GGTACC GGTAC^C
5987 GGTACC
MvnI 1927 CGCG CG^CG
5065 CGCG
NsiI 2086 ATGCAT ATGCA^T
4900 ATGCAT
PmaCI 871 CACGTG CAC^GTG
2335 CACGTG
PmlI 871 CACGTG CAC^GTG
2335 CACGTG
PstI 1363 CTGCAG CTGCA^G
3301 CTGCAG
SspBI 3161 TGTACA T^GTACA
6243 TGTACA
SspI 1504 AATATT AAT^ATT
3697 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
AccIII AciI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaI
ApaLI AspHI AspI AvaI AvaII
AviII BalI BanI BanII BbsI
BbvCI BbvI BciVI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaJI
BsaWI BseAI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstXI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DrdI DsaI EaeI
EarI EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MroI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeI NdeII NlaIII
NlaIV NspI PflFI PleI PpuMI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XcmI XhoII