##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Aor51HI ApaI AscI AseI
AsnI Asp718I BbeI BcgI BciVI
BfrI BsaBI BseAI BsiWI BspDI
BspEI BssHII Bst1107I BstZ17I ClaI
CpoI DraIII DrdI Eco47III EcoRV
EcoT22I FseI HincII HindII HpaI
KasI KpnI MamI MluI MroI
NarI NsiI PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacI SalI SapI SbfI SfiI
SfoI SnaBI SphI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AflIII 2071 ACATGT A^C[GA][CT]GT
AhdI 3837 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 3837 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1011 TGCGCA TGC^GCA
BclI 2361 TGATCA T^GATCA
BglI 141 GCCGTGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 3568 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3568 GCTCAGC GC^T[ACGT]AGC
Bsp1407I 639 TGTACA T^GTACA
BspLU11I 2071 ACATGT A^CATGT
BsrBI 4876 GAGCGG CCGCTC(-3/-3)
BsrGI 639 TGTACA T^GTACA
BstBI 6124 TTCGAA TT^CGAA
CelII 3568 GCTCAGC GC^T[ACGT]AGC
Eam1105I 3837 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 5604 GAATTC G^AATTC
FbaI 2361 TGATCA T^GATCA
FspI 1011 TGCGCA TGC^GCA
KspI 163 CCGCGG CCGC^GG
MfeI 4924 CAATTG C^AATTG
MunI 4924 CAATTG C^AATTG
NotI 135 GCGGCCGC GC^GGCCGC
NruI 182 TCGCGA TCG^CGA
NspV 6124 TTCGAA TT^CGAA
PaeR7I 2287 CTCGAG C^TCGAG
SacII 163 CCGCGG CCGC^GG
ScaI 2689 AGTACT AGT^ACT
SexAI 4245 ACCTGGT A^CC[AT]GGT
SfuI 6124 TTCGAA TT^CGAA
SgrAI 869 CACCGGCG C[GA]^CCGG[CT]G
SmaI 11 CCCGGG CCC^GGG
SspBI 639 TGTACA T^GTACA
SspI 5225 AATATT AAT^ATT
XbaI 1161 TCTAGA T^CTAGA
XhoI 2287 CTCGAG C^TCGAG
XmaCI 11 CCCGGG C^CCGGG
XmaI 11 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 1215 GTAGAC GT^[AC][GT]AC
3283 GTAGAC
Alw44I 715 GTGCAC G^TGCAC
6155 GTGCAC
AocI 342 CCTCAGG CC^T[ACGT]AGG
2628 CCTTAGG
ApaLI 715 GTGCAC G^TGCAC
6155 GTGCAC
Asp700I 2 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2369 GAATTTGTTC
AvrII 4021 CCTAGG C^CTAGG
4505 CCTAGG
BamHI 3690 GGATCC G^GATCC
4967 GGATCC
BbrPI 2533 CACGTG CAC^GTG
3441 CACGTG
BlnI 4021 CCTAGG C^CTAGG
4505 CCTAGG
BsaAI 2533 CACGTG [CT]AC^GT[GA]
3441 CACGTG
BsaWI 44 ACCGGA [AT]^CCGG[AT]
5930 TCCGGT
BsgI 2719 CTGCAC GTGCAG(16/14)
426 GTGCAG
BsiEI 136 CGGCCG CG[GA][CT]^CG
1547 CGGCCG
BsmI 23 GAATGC GAATGC(1/-1)
3623 GAATGC
BspHI 3663 TCATGA T^CATGA
3704 TCATGA
BsrDI 1531 GCAATG GCAATG(2/0)
5937 GCAATG
BssSI 2428 CACGAG CACGAG(-5/-1)
3906 CTCGTG
Bsu36I 342 CCTCAGG CC^T[ACGT]AGG
2628 CCTTAGG
EagI 136 CGGCCG C^GGCCG
1547 CGGCCG
EclXI 136 CGGCCG C^GGCCG
1547 CGGCCG
Eco52I 136 CGGCCG C^GGCCG
1547 CGGCCG
Eco81I 342 CCTCAGG CC^T[ACGT]AGG
2628 CCTTAGG
NcoI 316 CCATGG C^CATGG
563 CCATGG
NdeI 2095 CATATG CA^TATG
2667 CATATG
NheI 806 GCTAGC G^CTAGC
5184 GCTAGC
PmaCI 2533 CACGTG CAC^GTG
3441 CACGTG
PmlI 2533 CACGTG CAC^GTG
3441 CACGTG
RcaI 3663 TCATGA T^CATGA
3704 TCATGA
SpeI 3966 ACTAGT A^CTAGT
5203 ACTAGT
StuI 1784 AGGCCT AGG^CCT
4154 AGGCCT
XmnI 2 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2369 GAATTTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AspI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NspI PflFI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII