##################################
# NO CUT ENZYMES (   75 enzymes) #
##################################

AatII       AccIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        AspEI       AviII       AvrII       
BcgI        BclI        BlnI        BsaWI       BseAI       
BsiWI       BspDI       BspEI       BsrBI       BssHII      
Bst1107I    BstBI       BstZ17I     Bsu36I      ClaI        
CpoI        DraIII      DrdI        Eam1105I    Eco47III    
Eco81I      EcoT22I     FbaI        FseI        FspI        
KspI        MfeI        MluI        MroI        MunI        
NaeI        NgoMIV      NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PinAI       PmeI        
PshBI       PvuI        RsrII       SacII       SalI        
SapI        SbfI        SfiI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SspI        
SwaI        XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccI         1734  GTAGAC               GT^[AC][GT]AC
AccII        2662  CGCG                 CG^CG
AclI         1287  AACGTT               AA^CGTT
AcyI         2890  GGCGCC               G[GA]^CG[CT]C
AspI         4203  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         3083  CTCGGG               C^[CT]CG[GA]G
BamHI        3097  GGATCC               G^GATCC
BbeI         2890  GGCGCC               GGCGC^C
BbrPI        4583  CACGTG               CAC^GTG
BbvCI        3381  CCTCAGC              CCTCAGC(-5/-2)
BsaAI        4583  CACGTG               [CT]AC^GT[GA]
BsaHI        2890  GGCGCC               G[GA]^CG[CT]C
BsiEI        2975  CGGCCG               CG[GA][CT]^CG
BsmBI        3019  GAGACG               CGTCTC(1/5)
BsoBI        3083  CTCGGG               C^[CT]CG[GA]G
BsrDI        3062  CATTGC               GCAATG(2/0)
BsrFI        2010  GCCGGT               [GA]^CCGG[CT]
BstEII        828  GGTGACC              G^GT[ACGT]ACC
BstPI         828  GGTGACC              G^GT[ACGT]ACC
BstUI        2662  CGCG                 CG^CG
Cfr10I       2010  GCCGGT               [GA]^CCGG[CT]
DraI         5226  TTTAAA               TTT^AAA
EagI         2975  CGGCCG               C^GGCCG
EclXI        2975  CGGCCG               C^GGCCG
Eco52I       2975  CGGCCG               C^GGCCG
EcoNI        2072  CCTCACTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       828  GGTGACC              G^GT[ACGT]ACC
EcoRV        1443  GATATC               GAT^ATC
HaeII        2890  GGCGCC               [GA]GCGC^[CT]
HgaI         2936  GCGTC                GACGC(5/10)
Hin1I        2890  GGCGCC               G[GA]^CG[CT]C
KasI         2890  GGCGCC               G^GCGCC
MvnI         2662  CGCG                 CG^CG
NarI         2890  GGCGCC               GG^CGCC
NdeI         4134  CATATG               CA^TATG
NheI         4794  GCTAGC               G^CTAGC
PflFI        4203  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1045  CCATCTGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        4583  CACGTG               CAC^GTG
PmlI         4583  CACGTG               CAC^GTG
PshAI        4716  GACTGTTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     1287  AACGTT               AA^CGTT
SacI         2216  GAGCTC               GAGCT^C
SexAI        1123  ACCTGGT              A^CC[AT]GGT
SfoI         2890  GGCGCC               GGC^GCC
SphI         2364  GCATGC               GCATG^C
Tth111I      4203  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1045  CCATCTGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

Acc65I        715  GGTACC               G^GTACC
             1587  GGTACC              
AflII         726  CTTAAG               C^TTAAG
             2031  CTTAAG              
AflIII         19  ACATGT               A^C[GA][CT]GT
             5252  ACATGT              
AlwNI        1539  CAGGCACTG            CAG[ACGT][ACGT][ACGT]^CTG
             2569  CAGCAGCTG           
Asp718I       715  GGTACC               G^GTACC
             1587  GGTACC              
BcnI         1864  CCCGG                CC^[GC]GG
             3110  CCCGG               
BfrI          726  CTTAAG               C^TTAAG
             2031  CTTAAG              
BglI          435  GCCCGCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             4231  GCCTTACTGGC         
BsaBI          78  GATATTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2673  GATTCCAATC          
BsaI          629  GAGACC               GGTCTC(1/5)
             1041  GAGACC              
BsmI         2330  GAATGC               GAATGC(1/-1)
              933  GCATTC              
Bsp1407I     1226  TGTACA               T^GTACA
             5249  TGTACA              
BspLU11I       19  ACATGT               A^CATGT
             5252  ACATGT              
BsrGI        1226  TGTACA               T^GTACA
             5249  TGTACA              
BssSI        1194  CACGAG               CACGAG(-5/-1)
              555  CTCGTG              
BstAPI       2568  GCAGCAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2577  GCAAAGCTTGC         
HpaI          911  GTTAAC               GTT^AAC
             5123  GTTAAC              
KpnI          715  GGTACC               GGTAC^C
             1587  GGTACC              
MamI           78  GATATTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2673  GATTCCAATC          
NciI         1864  CCCGG                CC^[GC]GG
             3110  CCCGG               
ScaI          646  AGTACT               AGT^ACT
             4029  AGTACT              
SspBI        1226  TGTACA               T^GTACA
             5249  TGTACA              


#########################################
# 3 OR MORE CUT ENZYMES (  113 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        Asp700I     AspHI       AvaII       BalI        
BanI        BanII       BbsI        BbvI        BciVI       
BfaI        BglII       BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspHI       BspMI       BsrI        BssKI       
BstF5I      BstNI       BstXI       BstYI       Cac8I       
CelII       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoO109I    
EcoRI       EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HincII      HindII      HindIII     HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NcoI        NdeII       
NlaIII      NlaIV       NspI        PleI        PpuMI       
PstI        PvuII       RcaI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XcmI        XhoII       XmnI