################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AatII AccIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AviII AvrII BcgI BclI BlnI BsaWI BseAI BsiWI BspDI BspEI BsrBI BssHII Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DraIII DrdI Eam1105I Eco47III Eco81I EcoT22I FbaI FseI FspI KspI MfeI MluI MroI MunI NaeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshBI PvuI RsrII SacII SalI SapI SbfI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 1734 GTAGAC GT^[AC][GT]AC AccII 2662 CGCG CG^CG AclI 1287 AACGTT AA^CGTT AcyI 2890 GGCGCC G[GA]^CG[CT]C AspI 4203 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 3083 CTCGGG C^[CT]CG[GA]G BamHI 3097 GGATCC G^GATCC BbeI 2890 GGCGCC GGCGC^C BbrPI 4583 CACGTG CAC^GTG BbvCI 3381 CCTCAGC CCTCAGC(-5/-2) BsaAI 4583 CACGTG [CT]AC^GT[GA] BsaHI 2890 GGCGCC G[GA]^CG[CT]C BsiEI 2975 CGGCCG CG[GA][CT]^CG BsmBI 3019 GAGACG CGTCTC(1/5) BsoBI 3083 CTCGGG C^[CT]CG[GA]G BsrDI 3062 CATTGC GCAATG(2/0) BsrFI 2010 GCCGGT [GA]^CCGG[CT] BstEII 828 GGTGACC G^GT[ACGT]ACC BstPI 828 GGTGACC G^GT[ACGT]ACC BstUI 2662 CGCG CG^CG Cfr10I 2010 GCCGGT [GA]^CCGG[CT] DraI 5226 TTTAAA TTT^AAA EagI 2975 CGGCCG C^GGCCG EclXI 2975 CGGCCG C^GGCCG Eco52I 2975 CGGCCG C^GGCCG EcoNI 2072 CCTCACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 828 GGTGACC G^GT[ACGT]ACC EcoRV 1443 GATATC GAT^ATC HaeII 2890 GGCGCC [GA]GCGC^[CT] HgaI 2936 GCGTC GACGC(5/10) Hin1I 2890 GGCGCC G[GA]^CG[CT]C KasI 2890 GGCGCC G^GCGCC MvnI 2662 CGCG CG^CG NarI 2890 GGCGCC GG^CGCC NdeI 4134 CATATG CA^TATG NheI 4794 GCTAGC G^CTAGC PflFI 4203 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1045 CCATCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 4583 CACGTG CAC^GTG PmlI 4583 CACGTG CAC^GTG PshAI 4716 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 1287 AACGTT AA^CGTT SacI 2216 GAGCTC GAGCT^C SexAI 1123 ACCTGGT A^CC[AT]GGT SfoI 2890 GGCGCC GGC^GCC SphI 2364 GCATGC GCATG^C Tth111I 4203 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1045 CCATCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### Acc65I 715 GGTACC G^GTACC 1587 GGTACC AflII 726 CTTAAG C^TTAAG 2031 CTTAAG AflIII 19 ACATGT A^C[GA][CT]GT 5252 ACATGT AlwNI 1539 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG 2569 CAGCAGCTG Asp718I 715 GGTACC G^GTACC 1587 GGTACC BcnI 1864 CCCGG CC^[GC]GG 3110 CCCGG BfrI 726 CTTAAG C^TTAAG 2031 CTTAAG BglI 435 GCCCGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 4231 GCCTTACTGGC BsaBI 78 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2673 GATTCCAATC BsaI 629 GAGACC GGTCTC(1/5) 1041 GAGACC BsmI 2330 GAATGC GAATGC(1/-1) 933 GCATTC Bsp1407I 1226 TGTACA T^GTACA 5249 TGTACA BspLU11I 19 ACATGT A^CATGT 5252 ACATGT BsrGI 1226 TGTACA T^GTACA 5249 TGTACA BssSI 1194 CACGAG CACGAG(-5/-1) 555 CTCGTG BstAPI 2568 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2577 GCAAAGCTTGC HpaI 911 GTTAAC GTT^AAC 5123 GTTAAC KpnI 715 GGTACC GGTAC^C 1587 GGTACC MamI 78 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2673 GATTCCAATC NciI 1864 CCCGG CC^[GC]GG 3110 CCCGG ScaI 646 AGTACT AGT^ACT 4029 AGTACT SspBI 1226 TGTACA T^GTACA 5249 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 113 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI Asp700I AspHI AvaII BalI BanI BanII BbsI BbvI BciVI BfaI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BspMI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI