##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AccIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI AspEI AviII AvrII
BcgI BclI BlnI BsaWI BseAI
BsiWI BspDI BspEI BsrBI BssHII
Bst1107I BstBI BstZ17I Bsu36I ClaI
CpoI DraIII DrdI Eam1105I Eco47III
Eco81I EcoT22I FbaI FseI FspI
KspI MfeI MluI MroI MunI
NaeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshBI PvuI RsrII SacII SalI
SapI SbfI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
SwaI XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 1734 GTAGAC GT^[AC][GT]AC
AccII 2662 CGCG CG^CG
AclI 1287 AACGTT AA^CGTT
AcyI 2890 GGCGCC G[GA]^CG[CT]C
AspI 4203 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3083 CTCGGG C^[CT]CG[GA]G
BamHI 3097 GGATCC G^GATCC
BbeI 2890 GGCGCC GGCGC^C
BbrPI 4583 CACGTG CAC^GTG
BbvCI 3381 CCTCAGC CCTCAGC(-5/-2)
BsaAI 4583 CACGTG [CT]AC^GT[GA]
BsaHI 2890 GGCGCC G[GA]^CG[CT]C
BsiEI 2975 CGGCCG CG[GA][CT]^CG
BsmBI 3019 GAGACG CGTCTC(1/5)
BsoBI 3083 CTCGGG C^[CT]CG[GA]G
BsrDI 3062 CATTGC GCAATG(2/0)
BsrFI 2010 GCCGGT [GA]^CCGG[CT]
BstEII 828 GGTGACC G^GT[ACGT]ACC
BstPI 828 GGTGACC G^GT[ACGT]ACC
BstUI 2662 CGCG CG^CG
Cfr10I 2010 GCCGGT [GA]^CCGG[CT]
DraI 5226 TTTAAA TTT^AAA
EagI 2975 CGGCCG C^GGCCG
EclXI 2975 CGGCCG C^GGCCG
Eco52I 2975 CGGCCG C^GGCCG
EcoNI 2072 CCTCACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 828 GGTGACC G^GT[ACGT]ACC
EcoRV 1443 GATATC GAT^ATC
HaeII 2890 GGCGCC [GA]GCGC^[CT]
HgaI 2936 GCGTC GACGC(5/10)
Hin1I 2890 GGCGCC G[GA]^CG[CT]C
KasI 2890 GGCGCC G^GCGCC
MvnI 2662 CGCG CG^CG
NarI 2890 GGCGCC GG^CGCC
NdeI 4134 CATATG CA^TATG
NheI 4794 GCTAGC G^CTAGC
PflFI 4203 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1045 CCATCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 4583 CACGTG CAC^GTG
PmlI 4583 CACGTG CAC^GTG
PshAI 4716 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1287 AACGTT AA^CGTT
SacI 2216 GAGCTC GAGCT^C
SexAI 1123 ACCTGGT A^CC[AT]GGT
SfoI 2890 GGCGCC GGC^GCC
SphI 2364 GCATGC GCATG^C
Tth111I 4203 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1045 CCATCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Acc65I 715 GGTACC G^GTACC
1587 GGTACC
AflII 726 CTTAAG C^TTAAG
2031 CTTAAG
AflIII 19 ACATGT A^C[GA][CT]GT
5252 ACATGT
AlwNI 1539 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
2569 CAGCAGCTG
Asp718I 715 GGTACC G^GTACC
1587 GGTACC
BcnI 1864 CCCGG CC^[GC]GG
3110 CCCGG
BfrI 726 CTTAAG C^TTAAG
2031 CTTAAG
BglI 435 GCCCGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4231 GCCTTACTGGC
BsaBI 78 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2673 GATTCCAATC
BsaI 629 GAGACC GGTCTC(1/5)
1041 GAGACC
BsmI 2330 GAATGC GAATGC(1/-1)
933 GCATTC
Bsp1407I 1226 TGTACA T^GTACA
5249 TGTACA
BspLU11I 19 ACATGT A^CATGT
5252 ACATGT
BsrGI 1226 TGTACA T^GTACA
5249 TGTACA
BssSI 1194 CACGAG CACGAG(-5/-1)
555 CTCGTG
BstAPI 2568 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2577 GCAAAGCTTGC
HpaI 911 GTTAAC GTT^AAC
5123 GTTAAC
KpnI 715 GGTACC GGTAC^C
1587 GGTACC
MamI 78 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2673 GATTCCAATC
NciI 1864 CCCGG CC^[GC]GG
3110 CCCGG
ScaI 646 AGTACT AGT^ACT
4029 AGTACT
SspBI 1226 TGTACA T^GTACA
5249 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaII BalI
BanI BanII BbsI BbvI BciVI
BfaI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspHI BspMI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NcoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmnI