################################## # NO CUT ENZYMES ( 57 enzymes) # ################################## AflII AscI AseI AsnI AspI BamHI BbeI BciVI BfrI BglII BsaBI BsiWI BsrBI BsrDI Bst1107I BstAPI BstBI BstZ17I CpoI DraIII EcoRV EcoT22I HindIII KasI KspI MamI MfeI MluI MunI NarI NheI NotI NruI NsiI NspV PacI PflFI PmeI PshAI PshBI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SwaI Tth111I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AatII 4273 GACGTC GACGT^C AccIII 2548 TCCGGA T^CCGGA AclI 2501 AACGTT AA^CGTT AgeI 3785 ACCGGT A^CCGGT Aor51HI 1576 AGCGCT AGC^GCT ApaI 3993 GGGCCC GGGCC^C AvrII 412 CCTAGG C^CTAGG BbrPI 3119 CACGTG CAC^GTG BclI 1829 TGATCA T^GATCA BlnI 412 CCTAGG C^CTAGG BsaAI 3119 CACGTG [CT]AC^GT[GA] BseAI 2548 TCCGGA T^CCGGA BsmI 63 GCATTC GAATGC(1/-1) Bsp1407I 4313 TGTACA T^GTACA BspDI 3589 ATCGAT AT^CGAT BspEI 2548 TCCGGA T^CCGGA BsrGI 4313 TGTACA T^GTACA BssHII 2581 GCGCGC G^CGCGC ClaI 3589 ATCGAT AT^CGAT Eco47III 1576 AGCGCT AGC^GCT FbaI 1829 TGATCA T^GATCA FseI 5363 GGCCGGCC GGCCGG^CC HpaI 652 GTTAAC GTT^AAC MroI 2548 TCCGGA T^CCGGA NdeI 2455 CATATG CA^TATG PaeR7I 1314 CTCGAG C^TCGAG PinAI 3785 ACCGGT A^CCGGT PmaCI 3119 CACGTG CAC^GTG PmlI 3119 CACGTG CAC^GTG Psp1406I 2501 AACGTT AA^CGTT PvuI 4559 CGATCG CGAT^CG SacI 5592 GAGCTC GAGCT^C ScaI 3974 AGTACT AGT^ACT SphI 2207 GCATGC GCATG^C SspBI 4313 TGTACA T^GTACA SspI 4624 AATATT AAT^ATT XhoI 1314 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### Acc65I 1195 GGTACC G^GTACC 3405 GGTACC AccI 4751 GTCTAC GT^[AC][GT]AC 5193 GTCTAC AhdI 3609 GACGCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4268 GACAGGACGTC Alw44I 2162 GTGCAC G^TGCAC 4725 GTGCAC ApaLI 2162 GTGCAC G^TGCAC 4725 GTGCAC Asp700I 14 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3783 GAACCGGTTC Asp718I 1195 GGTACC G^GTACC 3405 GGTACC AspEI 3609 GACGCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4268 GACAGGACGTC BsaI 2413 GGTCTC GGTCTC(1/5) 5689 GGTCTC BsaWI 2548 TCCGGA [AT]^CCGG[AT] 3785 ACCGGT BsmBI 4275 CGTCTC CGTCTC(1/5) 1339 GAGACG BstEII 1379 GGTCACC G^GT[ACGT]ACC 1986 GGTCACC BstPI 1379 GGTCACC G^GT[ACGT]ACC 1986 GGTCACC DrdI 492 GACTGATGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3232 GACCCTGATGTC Eam1105I 3609 GACGCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4268 GACAGGACGTC EcoO65I 1379 GGTCACC G^GT[ACGT]ACC 1986 GGTCACC EcoRI 3178 GAATTC G^AATTC 5086 GAATTC KpnI 1195 GGTACC GGTAC^C 3405 GGTACC PflMI 929 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1670 CCAGCCAGTGG SapI 1568 GAAGAGC GCTCTTC(1/4) 2101 GAAGAGC TaqI 1315 TCGA T^CGA 3590 TCGA TthHB8I 1315 TCGA T^CGA 3590 TCGA Van91I 929 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1670 CCAGCCAGTGG XbaI 294 TCTAGA T^CTAGA 2227 TCTAGA XmnI 14 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3783 GAACCGGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 139 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI AocI ApoI AspHI AvaI AvaII AviII BalI BanI BanII BbsI BbvCI BbvI BcgI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspLU11I BspMI BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EagI EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PleI PpuMI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XcmI XhoII