##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AflII AscI AseI AsnI AspI
BamHI BbeI BciVI BfrI BglII
BsaBI BsiWI BsrBI BsrDI Bst1107I
BstAPI BstBI BstZ17I CpoI DraIII
EcoRV EcoT22I HindIII KasI KspI
MamI MfeI MluI MunI NarI
NheI NotI NruI NsiI NspV
PacI PflFI PmeI PshAI PshBI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SwaI Tth111I
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 4273 GACGTC GACGT^C
AccIII 2548 TCCGGA T^CCGGA
AclI 2501 AACGTT AA^CGTT
AgeI 3785 ACCGGT A^CCGGT
Aor51HI 1576 AGCGCT AGC^GCT
ApaI 3993 GGGCCC GGGCC^C
AvrII 412 CCTAGG C^CTAGG
BbrPI 3119 CACGTG CAC^GTG
BclI 1829 TGATCA T^GATCA
BlnI 412 CCTAGG C^CTAGG
BsaAI 3119 CACGTG [CT]AC^GT[GA]
BseAI 2548 TCCGGA T^CCGGA
BsmI 63 GCATTC GAATGC(1/-1)
Bsp1407I 4313 TGTACA T^GTACA
BspDI 3589 ATCGAT AT^CGAT
BspEI 2548 TCCGGA T^CCGGA
BsrGI 4313 TGTACA T^GTACA
BssHII 2581 GCGCGC G^CGCGC
ClaI 3589 ATCGAT AT^CGAT
Eco47III 1576 AGCGCT AGC^GCT
FbaI 1829 TGATCA T^GATCA
FseI 5363 GGCCGGCC GGCCGG^CC
HpaI 652 GTTAAC GTT^AAC
MroI 2548 TCCGGA T^CCGGA
NdeI 2455 CATATG CA^TATG
PaeR7I 1314 CTCGAG C^TCGAG
PinAI 3785 ACCGGT A^CCGGT
PmaCI 3119 CACGTG CAC^GTG
PmlI 3119 CACGTG CAC^GTG
Psp1406I 2501 AACGTT AA^CGTT
PvuI 4559 CGATCG CGAT^CG
SacI 5592 GAGCTC GAGCT^C
ScaI 3974 AGTACT AGT^ACT
SphI 2207 GCATGC GCATG^C
SspBI 4313 TGTACA T^GTACA
SspI 4624 AATATT AAT^ATT
XhoI 1314 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Acc65I 1195 GGTACC G^GTACC
3405 GGTACC
AccI 4751 GTCTAC GT^[AC][GT]AC
5193 GTCTAC
AhdI 3609 GACGCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4268 GACAGGACGTC
Alw44I 2162 GTGCAC G^TGCAC
4725 GTGCAC
ApaLI 2162 GTGCAC G^TGCAC
4725 GTGCAC
Asp700I 14 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3783 GAACCGGTTC
Asp718I 1195 GGTACC G^GTACC
3405 GGTACC
AspEI 3609 GACGCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4268 GACAGGACGTC
BsaI 2413 GGTCTC GGTCTC(1/5)
5689 GGTCTC
BsaWI 2548 TCCGGA [AT]^CCGG[AT]
3785 ACCGGT
BsmBI 4275 CGTCTC CGTCTC(1/5)
1339 GAGACG
BstEII 1379 GGTCACC G^GT[ACGT]ACC
1986 GGTCACC
BstPI 1379 GGTCACC G^GT[ACGT]ACC
1986 GGTCACC
DrdI 492 GACTGATGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3232 GACCCTGATGTC
Eam1105I 3609 GACGCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4268 GACAGGACGTC
EcoO65I 1379 GGTCACC G^GT[ACGT]ACC
1986 GGTCACC
EcoRI 3178 GAATTC G^AATTC
5086 GAATTC
KpnI 1195 GGTACC GGTAC^C
3405 GGTACC
PflMI 929 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1670 CCAGCCAGTGG
SapI 1568 GAAGAGC GCTCTTC(1/4)
2101 GAAGAGC
TaqI 1315 TCGA T^CGA
3590 TCGA
TthHB8I 1315 TCGA T^CGA
3590 TCGA
Van91I 929 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1670 CCAGCCAGTGG
XbaI 294 TCTAGA T^CTAGA
2227 TCTAGA
XmnI 14 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3783 GAACCGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI AspHI AvaI AvaII AviII
BalI BanI BanII BbsI BbvCI
BbvI BcgI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspHI BspLU11I BspMI
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EagI EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XcmI XhoII