##################################
# NO CUT ENZYMES ( 43 enzymes) #
##################################
Aor51HI Asp700I AviII BamHI BbeI
BclI BsiEI BsiWI BspHI BspLU11I
BstXI CpoI EagI EclXI Eco47III
Eco52I EcoT22I FbaI FseI FspI
HpaI KasI MluI NarI NheI
NotI NruI NsiI PacI PmeI
PvuI RcaI RsrII SfiI SfoI
SgrAI SmaI SnaBI SpeI SwaI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 5527 GGTACC G^GTACC
AccIII 390 TCCGGA T^CCGGA
AclI 5633 AACGTT AA^CGTT
AflII 3995 CTTAAG C^TTAAG
AgeI 2316 ACCGGT A^CCGGT
ApaI 6155 GGGCCC GGGCC^C
AscI 183 GGCGCGCC GG^CGCGCC
Asp718I 5527 GGTACC G^GTACC
AvrII 5502 CCTAGG C^CTAGG
BbrPI 3948 CACGTG CAC^GTG
BciVI 5234 GTATCC GTATCC(6/5)
BfrI 3995 CTTAAG C^TTAAG
BlnI 5502 CCTAGG C^CTAGG
BsaBI 767 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 390 TCCGGA T^CCGGA
BsmI 471 GAATGC GAATGC(1/-1)
BspDI 1002 ATCGAT AT^CGAT
BspEI 390 TCCGGA T^CCGGA
BsrDI 1564 GCAATG GCAATG(2/0)
BssHII 184 GCGCGC G^CGCGC
Bst1107I 4601 GTATAC GTA^TAC
BstBI 4793 TTCGAA TT^CGAA
BstEII 2312 GGTGACC G^GT[ACGT]ACC
BstPI 2312 GGTGACC G^GT[ACGT]ACC
BstZ17I 4601 GTATAC GTA^TAC
ClaI 1002 ATCGAT AT^CGAT
EcoO65I 2312 GGTGACC G^GT[ACGT]ACC
EcoRI 834 GAATTC G^AATTC
KpnI 5527 GGTACC GGTAC^C
KspI 173 CCGCGG CCGC^GG
MamI 767 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1528 CAATTG C^AATTG
MroI 390 TCCGGA T^CCGGA
MunI 1528 CAATTG C^AATTG
NaeI 6202 GCCGGC GCC^GGC
NdeI 589 CATATG CA^TATG
NgoMIV 6202 GCCGGC G^CCGGC
NspV 4793 TTCGAA TT^CGAA
PinAI 2316 ACCGGT A^CCGGT
PmaCI 3948 CACGTG CAC^GTG
PmlI 3948 CACGTG CAC^GTG
PshAI 2870 GACTCGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 5633 AACGTT AA^CGTT
SacII 173 CCGCGG CCGC^GG
SalI 443 GTCGAC G^TCGAC
SbfI 3326 CCTGCAGG CCTGCA^GG
ScaI 5202 AGTACT AGT^ACT
SexAI 94 ACCAGGT A^CC[AT]GGT
SfuI 4793 TTCGAA TT^CGAA
Sse8387I 3326 CCTGCAGG CCTGCA^GG
SspI 3823 AATATT AAT^ATT
XbaI 4559 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AatII 669 GACGTC GACGT^C
2862 GACGTC
AccI 443 GTCGAC GT^[AC][GT]AC
4601 GTATAC
AflIII 3947 ACACGT A^C[GA][CT]GT
3949 ACGTGT
AhdI 1947 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2001 GACGAGCTGTC
Alw44I 3082 GTGCAC G^TGCAC
4655 GTGCAC
ApaLI 3082 GTGCAC G^TGCAC
4655 GTGCAC
AseI 2613 ATTAAT AT^TAAT
5062 ATTAAT
AsnI 2613 ATTAAT AT^TAAT
5062 ATTAAT
AspEI 1947 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2001 GACGAGCTGTC
BglII 2938 AGATCT A^GATCT
3008 AGATCT
BsrBI 4095 CCGCTC CCGCTC(-3/-3)
3912 GAGCGG
BstAPI 272 GCATCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1790 GCAGCAGATGC
Eam1105I 1947 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2001 GACGAGCTGTC
EcoNI 506 CCTGAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4712 CCTGACACAGG
EcoRV 3441 GATATC GAT^ATC
4263 GATATC
HincII 443 GTCGAC GT[CT]^[GA]AC
1938 GTCAAC
HindII 443 GTCGAC GT[CT]^[GA]AC
1938 GTCAAC
HindIII 2682 AAGCTT A^AGCTT
3498 AAGCTT
PaeR7I 790 CTCGAG C^TCGAG
2872 CTCGAG
PshBI 2613 ATTAAT AT^TAAT
5062 ATTAAT
SacI 1626 GAGCTC GAGCT^C
3173 GAGCTC
XcmI 2026 CCATGCTGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2060 CCAGGAGGTCAGTGG
XhoI 790 CTCGAG C^TCGAG
2872 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
AspHI AspI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcgI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrFI BsrGI BsrI BssKI
BssSI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DraIII DrdI DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XhoII