################################## # NO CUT ENZYMES ( 43 enzymes) # ################################## Aor51HI Asp700I AviII BamHI BbeI BclI BsiEI BsiWI BspHI BspLU11I BstXI CpoI EagI EclXI Eco47III Eco52I EcoT22I FbaI FseI FspI HpaI KasI MluI NarI NheI NotI NruI NsiI PacI PmeI PvuI RcaI RsrII SfiI SfoI SgrAI SmaI SnaBI SpeI SwaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Acc65I 5527 GGTACC G^GTACC AccIII 390 TCCGGA T^CCGGA AclI 5633 AACGTT AA^CGTT AflII 3995 CTTAAG C^TTAAG AgeI 2316 ACCGGT A^CCGGT ApaI 6155 GGGCCC GGGCC^C AscI 183 GGCGCGCC GG^CGCGCC Asp718I 5527 GGTACC G^GTACC AvrII 5502 CCTAGG C^CTAGG BbrPI 3948 CACGTG CAC^GTG BciVI 5234 GTATCC GTATCC(6/5) BfrI 3995 CTTAAG C^TTAAG BlnI 5502 CCTAGG C^CTAGG BsaBI 767 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 390 TCCGGA T^CCGGA BsmI 471 GAATGC GAATGC(1/-1) BspDI 1002 ATCGAT AT^CGAT BspEI 390 TCCGGA T^CCGGA BsrDI 1564 GCAATG GCAATG(2/0) BssHII 184 GCGCGC G^CGCGC Bst1107I 4601 GTATAC GTA^TAC BstBI 4793 TTCGAA TT^CGAA BstEII 2312 GGTGACC G^GT[ACGT]ACC BstPI 2312 GGTGACC G^GT[ACGT]ACC BstZ17I 4601 GTATAC GTA^TAC ClaI 1002 ATCGAT AT^CGAT EcoO65I 2312 GGTGACC G^GT[ACGT]ACC EcoRI 834 GAATTC G^AATTC KpnI 5527 GGTACC GGTAC^C KspI 173 CCGCGG CCGC^GG MamI 767 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1528 CAATTG C^AATTG MroI 390 TCCGGA T^CCGGA MunI 1528 CAATTG C^AATTG NaeI 6202 GCCGGC GCC^GGC NdeI 589 CATATG CA^TATG NgoMIV 6202 GCCGGC G^CCGGC NspV 4793 TTCGAA TT^CGAA PinAI 2316 ACCGGT A^CCGGT PmaCI 3948 CACGTG CAC^GTG PmlI 3948 CACGTG CAC^GTG PshAI 2870 GACTCGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 5633 AACGTT AA^CGTT SacII 173 CCGCGG CCGC^GG SalI 443 GTCGAC G^TCGAC SbfI 3326 CCTGCAGG CCTGCA^GG ScaI 5202 AGTACT AGT^ACT SexAI 94 ACCAGGT A^CC[AT]GGT SfuI 4793 TTCGAA TT^CGAA Sse8387I 3326 CCTGCAGG CCTGCA^GG SspI 3823 AATATT AAT^ATT XbaI 4559 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AatII 669 GACGTC GACGT^C 2862 GACGTC AccI 443 GTCGAC GT^[AC][GT]AC 4601 GTATAC AflIII 3947 ACACGT A^C[GA][CT]GT 3949 ACGTGT AhdI 1947 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2001 GACGAGCTGTC Alw44I 3082 GTGCAC G^TGCAC 4655 GTGCAC ApaLI 3082 GTGCAC G^TGCAC 4655 GTGCAC AseI 2613 ATTAAT AT^TAAT 5062 ATTAAT AsnI 2613 ATTAAT AT^TAAT 5062 ATTAAT AspEI 1947 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2001 GACGAGCTGTC BglII 2938 AGATCT A^GATCT 3008 AGATCT BsrBI 4095 CCGCTC CCGCTC(-3/-3) 3912 GAGCGG BstAPI 272 GCATCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1790 GCAGCAGATGC Eam1105I 1947 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2001 GACGAGCTGTC EcoNI 506 CCTGAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4712 CCTGACACAGG EcoRV 3441 GATATC GAT^ATC 4263 GATATC HincII 443 GTCGAC GT[CT]^[GA]AC 1938 GTCAAC HindII 443 GTCGAC GT[CT]^[GA]AC 1938 GTCAAC HindIII 2682 AAGCTT A^AGCTT 3498 AAGCTT PaeR7I 790 CTCGAG C^TCGAG 2872 CTCGAG PshBI 2613 ATTAAT AT^TAAT 5062 ATTAAT SacI 1626 GAGCTC GAGCT^C 3173 GAGCTC XcmI 2026 CCATGCTGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2060 CCAGGAGGTCAGTGG XhoI 790 CTCGAG C^TCGAG 2872 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 140 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApoI AspHI AspI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcgI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrFI BsrGI BsrI BssKI BssSI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DraIII DrdI DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XhoII