################################## # NO CUT ENZYMES ( 77 enzymes) # ################################## AatII AccII AccIII AcyI AgeI AscI AseI AsnI Asp700I AvrII BbeI BbrPI BciVI BclI BlnI BsaAI BsaHI BseAI BsiEI BsiWI BspDI BspEI BsrBI BssHII BssSI Bst1107I BstEII BstPI BstUI BstZ17I ClaI CpoI DrdI DsaI EagI EclXI Eco52I EcoO65I EcoRI EcoRV FbaI FseI Hin1I HpaI KasI KspI MfeI MluI MroI MunI MvnI NarI NcoI NdeI NheI NotI NruI PacI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RsrII SacII SalI ScaI SfiI SfoI SgrAI SnaBI SpeI SwaI XbaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccI 2607 GTCTAC GT^[AC][GT]AC AclI 3297 AACGTT AA^CGTT AflIII 2581 ACATGT A^C[GA][CT]GT Alw44I 2355 GTGCAC G^TGCAC ApaLI 2355 GTGCAC G^TGCAC AspI 1960 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1843 TGCGCA TGC^GCA BamHI 3161 GGATCC G^GATCC BcgI 802 GCAGCCTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 1399 AGATCT A^GATCT BsaBI 1853 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 607 TCCGGT [AT]^CCGG[AT] Bsp1407I 3044 TGTACA T^GTACA BspLU11I 2581 ACATGT A^CATGT BsrFI 1500 GCCGGC [GA]^CCGG[CT] BsrGI 3044 TGTACA T^GTACA BstAPI 1298 GCAGCTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 915 TTCGAA TT^CGAA BstXI 3608 CCATCCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 1500 GCCGGC [GA]^CCGG[CT] DraIII 260 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 3660 CCTAGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FspI 1843 TGCGCA TGC^GCA HindIII 3065 AAGCTT A^AGCTT MamI 1853 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 1500 GCCGGC GCC^GGC NgoMIV 1500 GCCGGC G^CCGGC NspV 915 TTCGAA TT^CGAA PaeR7I 454 CTCGAG C^TCGAG PflFI 1960 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC Psp1406I 3297 AACGTT AA^CGTT SexAI 2028 ACCAGGT A^CC[AT]GGT SfuI 915 TTCGAA TT^CGAA SphI 1255 GCATGC GCATG^C SspBI 3044 TGTACA T^GTACA SspI 1249 AATATT AAT^ATT StuI 4352 AGGCCT AGG^CCT Tth111I 1960 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 454 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### Acc65I 2392 GGTACC G^GTACC 4573 GGTACC AflII 3129 CTTAAG C^TTAAG 3740 CTTAAG AhdI 866 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2510 GACTCTGAGTC Aor51HI 120 AGCGCT AGC^GCT 1617 AGCGCT Asp718I 2392 GGTACC G^GTACC 4573 GGTACC AspEI 866 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2510 GACTCTGAGTC BfrI 3129 CTTAAG C^TTAAG 3740 CTTAAG BglI 626 GCCTCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 805 GCCTGTTCGGC BsmBI 2264 GAGACG CGTCTC(1/5) 2756 GAGACG BsmI 3764 GAATGC GAATGC(1/-1) 4242 GCATTC BsrDI 348 CATTGC GCAATG(2/0) 4342 CATTGC BstYI 1399 AGATCT [GA]^GATC[CT] 3161 GGATCC DraI 2864 TTTAAA TTT^AAA 4191 TTTAAA Eam1105I 866 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2510 GACTCTGAGTC Eco47III 120 AGCGCT AGC^GCT 1617 AGCGCT EcoT22I 765 ATGCAT ATGCA^T 2105 ATGCAT FauI 2378 CCCGC CCCGC(4/6) 223 GCGGG HaeII 120 AGCGCT [GA]GCGC^[CT] 1617 AGCGCT HincII 926 GTCAAC GT[CT]^[GA]AC 3375 GTTGAC HindII 926 GTCAAC GT[CT]^[GA]AC 3375 GTTGAC KpnI 2392 GGTACC GGTAC^C 4573 GGTACC MaeII 1416 ACGT A^CGT 3298 ACGT MflI 1399 AGATCT [GA]^GATC[CT] 3161 GGATCC NsiI 765 ATGCAT ATGCA^T 2105 ATGCAT PflMI 2345 CCAGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2967 CCACCACCTGG PpuMI 2494 AGGACCT [GA]G^G[AT]CC[CT] 3049 AGGACCC SbfI 1976 CCTGCAGG CCTGCA^GG 2987 CCTGCAGG SmaI 273 CCCGGG CCC^GGG 2233 CCCGGG Sse8387I 1976 CCTGCAGG CCTGCA^GG 2987 CCTGCAGG TaqI 455 TCGA T^CGA 916 TCGA TthHB8I 455 TCGA T^CGA 916 TCGA Van91I 2345 CCAGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2967 CCACCACCTGG XcmI 2559 CCAATGGCTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4055 CCAGTCTTCCCCTGG XhoII 1399 AGATCT [GA]^GATC[CT] 3161 GGATCC XmaCI 273 CCCGGG C^CCGGG 2233 CCCGGG XmaI 273 CCCGGG C^CCGGG 2233 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI AocI ApaI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspMI BsrI BssKI BstF5I BstNI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII EaeI EarI Eco81I EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI PvuII RcaI RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI