##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccII AccIII AcyI AgeI
AscI AseI AsnI Asp700I AvrII
BbeI BbrPI BciVI BclI BlnI
BsaAI BsaHI BseAI BsiEI BsiWI
BspDI BspEI BsrBI BssHII BssSI
Bst1107I BstEII BstPI BstUI BstZ17I
ClaI CpoI DrdI DsaI EagI
EclXI Eco52I EcoO65I EcoRI EcoRV
FbaI FseI Hin1I HpaI KasI
KspI MfeI MluI MroI MunI
MvnI NarI NcoI NdeI NheI
NotI NruI PacI PinAI PmaCI
PmeI PmlI PshAI PshBI PvuI
RsrII SacII SalI ScaI SfiI
SfoI SgrAI SnaBI SpeI SwaI
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 2607 GTCTAC GT^[AC][GT]AC
AclI 3297 AACGTT AA^CGTT
AflIII 2581 ACATGT A^C[GA][CT]GT
Alw44I 2355 GTGCAC G^TGCAC
ApaLI 2355 GTGCAC G^TGCAC
AspI 1960 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1843 TGCGCA TGC^GCA
BamHI 3161 GGATCC G^GATCC
BcgI 802 GCAGCCTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1399 AGATCT A^GATCT
BsaBI 1853 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 607 TCCGGT [AT]^CCGG[AT]
Bsp1407I 3044 TGTACA T^GTACA
BspLU11I 2581 ACATGT A^CATGT
BsrFI 1500 GCCGGC [GA]^CCGG[CT]
BsrGI 3044 TGTACA T^GTACA
BstAPI 1298 GCAGCTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 915 TTCGAA TT^CGAA
BstXI 3608 CCATCCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1500 GCCGGC [GA]^CCGG[CT]
DraIII 260 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 3660 CCTAGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 1843 TGCGCA TGC^GCA
HindIII 3065 AAGCTT A^AGCTT
MamI 1853 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1500 GCCGGC GCC^GGC
NgoMIV 1500 GCCGGC G^CCGGC
NspV 915 TTCGAA TT^CGAA
PaeR7I 454 CTCGAG C^TCGAG
PflFI 1960 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 3297 AACGTT AA^CGTT
SexAI 2028 ACCAGGT A^CC[AT]GGT
SfuI 915 TTCGAA TT^CGAA
SphI 1255 GCATGC GCATG^C
SspBI 3044 TGTACA T^GTACA
SspI 1249 AATATT AAT^ATT
StuI 4352 AGGCCT AGG^CCT
Tth111I 1960 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 454 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 2392 GGTACC G^GTACC
4573 GGTACC
AflII 3129 CTTAAG C^TTAAG
3740 CTTAAG
AhdI 866 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2510 GACTCTGAGTC
Aor51HI 120 AGCGCT AGC^GCT
1617 AGCGCT
Asp718I 2392 GGTACC G^GTACC
4573 GGTACC
AspEI 866 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2510 GACTCTGAGTC
BfrI 3129 CTTAAG C^TTAAG
3740 CTTAAG
BglI 626 GCCTCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
805 GCCTGTTCGGC
BsmBI 2264 GAGACG CGTCTC(1/5)
2756 GAGACG
BsmI 3764 GAATGC GAATGC(1/-1)
4242 GCATTC
BsrDI 348 CATTGC GCAATG(2/0)
4342 CATTGC
BstYI 1399 AGATCT [GA]^GATC[CT]
3161 GGATCC
DraI 2864 TTTAAA TTT^AAA
4191 TTTAAA
Eam1105I 866 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2510 GACTCTGAGTC
Eco47III 120 AGCGCT AGC^GCT
1617 AGCGCT
EcoT22I 765 ATGCAT ATGCA^T
2105 ATGCAT
FauI 2378 CCCGC CCCGC(4/6)
223 GCGGG
HaeII 120 AGCGCT [GA]GCGC^[CT]
1617 AGCGCT
HincII 926 GTCAAC GT[CT]^[GA]AC
3375 GTTGAC
HindII 926 GTCAAC GT[CT]^[GA]AC
3375 GTTGAC
KpnI 2392 GGTACC GGTAC^C
4573 GGTACC
MaeII 1416 ACGT A^CGT
3298 ACGT
MflI 1399 AGATCT [GA]^GATC[CT]
3161 GGATCC
NsiI 765 ATGCAT ATGCA^T
2105 ATGCAT
PflMI 2345 CCAGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2967 CCACCACCTGG
PpuMI 2494 AGGACCT [GA]G^G[AT]CC[CT]
3049 AGGACCC
SbfI 1976 CCTGCAGG CCTGCA^GG
2987 CCTGCAGG
SmaI 273 CCCGGG CCC^GGG
2233 CCCGGG
Sse8387I 1976 CCTGCAGG CCTGCA^GG
2987 CCTGCAGG
TaqI 455 TCGA T^CGA
916 TCGA
TthHB8I 455 TCGA T^CGA
916 TCGA
Van91I 2345 CCAGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2967 CCACCACCTGG
XcmI 2559 CCAATGGCTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4055 CCAGTCTTCCCCTGG
XhoII 1399 AGATCT [GA]^GATC[CT]
3161 GGATCC
XmaCI 273 CCCGGG C^CCGGG
2233 CCCGGG
XmaI 273 CCCGGG C^CCGGG
2233 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApaI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspHI BspMI BsrI BssKI
BstF5I BstNI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RcaI
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI