################################## # NO CUT ENZYMES ( 86 enzymes) # ################################## Acc65I AccIII AclI AflIII AgeI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AvaI BamHI BbeI BbrPI BbvCI BglII BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspEI BspHI BspLU11I BsrBI BsrGI BssSI BstBI BstEII BstPI CpoI DraIII EagI EclXI Eco47III Eco52I EcoO65I FseI HaeII HgaI HincII HindII HpaI KasI KpnI KspI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SspBI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AatII 2943 GACGTC GACGT^C AccI 5265 GTATAC GT^[AC][GT]AC AcyI 2943 GACGTC G[GA]^CG[CT]C AflII 4107 CTTAAG C^TTAAG AhdI 2943 GACGTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 375 CCTAAGG CC^T[ACGT]AGG AseI 3289 ATTAAT AT^TAAT AsnI 3289 ATTAAT AT^TAAT AspEI 2943 GACGTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 3970 TGCGCA TGC^GCA AvrII 1386 CCTAGG C^CTAGG BalI 3011 TGGCCA TGG^CCA BanI 2579 GGCACC G^G[CT][GA]CC BcgI 3584 CGATCATAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1738 GTATCC GTATCC(6/5) BfrI 4107 CTTAAG C^TTAAG BglI 1938 GCCCATCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 1386 CCTAGG C^CTAGG BsaBI 2436 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 2943 GACGTC G[GA]^CG[CT]C BsaWI 2023 ACCGGA [AT]^CCGG[AT] BspDI 2338 ATCGAT AT^CGAT BsrFI 3082 GCCGGT [GA]^CCGG[CT] Bst1107I 5265 GTATAC GTA^TAC BstZ17I 5265 GTATAC GTA^TAC Bsu36I 375 CCTAAGG CC^T[ACGT]AGG Cfr10I 3082 GCCGGT [GA]^CCGG[CT] ClaI 2338 ATCGAT AT^CGAT DrdI 4376 GACAGACCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 2943 GACGTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 375 CCTAAGG CC^T[ACGT]AGG EcoRI 1 GAATTC G^AATTC EcoRV 5291 GATATC GAT^ATC FspI 3970 TGCGCA TGC^GCA Hin1I 2943 GACGTC G[GA]^CG[CT]C HindIII 2530 AAGCTT A^AGCTT MamI 2436 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 3011 TGGCCA TGG^CCA MscI 3011 TGGCCA TGG^CCA PshBI 3289 ATTAAT AT^TAAT StuI 184 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### BcnI 1002 CCGGG CC^[GC]GG 5419 CCGGG BlpI 153 GCTGAGC GC^T[ACGT]AGC 3761 GCTTAGC Bpu1102I 153 GCTGAGC GC^T[ACGT]AGC 3761 GCTTAGC BsaAI 685 CACGTA [CT]AC^GT[GA] 3861 TACGTG BsgI 243 GTGCAG GTGCAG(16/14) 1072 GTGCAG CelII 153 GCTGAGC GC^T[ACGT]AGC 3761 GCTTAGC EaeI 1123 TGGCCG [CT]^GGCC[GA] 3011 TGGCCA EcoNI 1337 CCTCCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2284 CCTATGGCAGG EcoT22I 3517 ATGCAT ATGCA^T 5325 ATGCAT FauI 1952 CCCGC CCCGC(4/6) 2766 CCCGC MslI 3067 CATCAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 3095 CATGATCATG NciI 1002 CCGGG CC^[GC]GG 5419 CCGGG NsiI 3517 ATGCAT ATGCA^T 5325 ATGCAT PflMI 664 CCAGTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5448 CCAGCACTTGG PstI 964 CTGCAG CTGCA^G 973 CTGCAG SacI 3805 GAGCTC GAGCT^C 4420 GAGCTC SapI 3329 GAAGAGC GCTCTTC(1/4) 4565 GCTCTTC ScaI 3786 AGTACT AGT^ACT 4359 AGTACT SphI 1278 GCATGC GCATG^C 2381 GCATGC SspI 2274 AATATT AAT^ATT 5002 AATATT Van91I 664 CCAGTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5448 CCAGCACTTGG XbaI 2628 TCTAGA T^CTAGA 4570 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AspI AvaII BanII BbsI BbvI BclI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrDI BsrI BssHII BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EarI EcoO109I EcoRII EcoT14I FbaI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NcoI NdeII NheI NlaIII NlaIV NspI PflFI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SpeI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XcmI XhoII XmnI