##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
Acc65I AccIII AclI AflIII AgeI
Alw44I Aor51HI ApaI ApaLI AscI
Asp718I AvaI BamHI BbeI BbrPI
BbvCI BglII BseAI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspEI BspHI
BspLU11I BsrBI BsrGI BssSI BstBI
BstEII BstPI CpoI DraIII EagI
EclXI Eco47III Eco52I EcoO65I FseI
HaeII HgaI HincII HindII HpaI
KasI KpnI KspI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I SspBI SwaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 2943 GACGTC GACGT^C
AccI 5265 GTATAC GT^[AC][GT]AC
AcyI 2943 GACGTC G[GA]^CG[CT]C
AflII 4107 CTTAAG C^TTAAG
AhdI 2943 GACGTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 375 CCTAAGG CC^T[ACGT]AGG
AseI 3289 ATTAAT AT^TAAT
AsnI 3289 ATTAAT AT^TAAT
AspEI 2943 GACGTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3970 TGCGCA TGC^GCA
AvrII 1386 CCTAGG C^CTAGG
BalI 3011 TGGCCA TGG^CCA
BanI 2579 GGCACC G^G[CT][GA]CC
BcgI 3584 CGATCATAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1738 GTATCC GTATCC(6/5)
BfrI 4107 CTTAAG C^TTAAG
BglI 1938 GCCCATCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1386 CCTAGG C^CTAGG
BsaBI 2436 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2943 GACGTC G[GA]^CG[CT]C
BsaWI 2023 ACCGGA [AT]^CCGG[AT]
BspDI 2338 ATCGAT AT^CGAT
BsrFI 3082 GCCGGT [GA]^CCGG[CT]
Bst1107I 5265 GTATAC GTA^TAC
BstZ17I 5265 GTATAC GTA^TAC
Bsu36I 375 CCTAAGG CC^T[ACGT]AGG
Cfr10I 3082 GCCGGT [GA]^CCGG[CT]
ClaI 2338 ATCGAT AT^CGAT
DrdI 4376 GACAGACCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2943 GACGTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 375 CCTAAGG CC^T[ACGT]AGG
EcoRI 1 GAATTC G^AATTC
EcoRV 5291 GATATC GAT^ATC
FspI 3970 TGCGCA TGC^GCA
Hin1I 2943 GACGTC G[GA]^CG[CT]C
HindIII 2530 AAGCTT A^AGCTT
MamI 2436 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3011 TGGCCA TGG^CCA
MscI 3011 TGGCCA TGG^CCA
PshBI 3289 ATTAAT AT^TAAT
StuI 184 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
BcnI 1002 CCGGG CC^[GC]GG
5419 CCGGG
BlpI 153 GCTGAGC GC^T[ACGT]AGC
3761 GCTTAGC
Bpu1102I 153 GCTGAGC GC^T[ACGT]AGC
3761 GCTTAGC
BsaAI 685 CACGTA [CT]AC^GT[GA]
3861 TACGTG
BsgI 243 GTGCAG GTGCAG(16/14)
1072 GTGCAG
CelII 153 GCTGAGC GC^T[ACGT]AGC
3761 GCTTAGC
EaeI 1123 TGGCCG [CT]^GGCC[GA]
3011 TGGCCA
EcoNI 1337 CCTCCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2284 CCTATGGCAGG
EcoT22I 3517 ATGCAT ATGCA^T
5325 ATGCAT
FauI 1952 CCCGC CCCGC(4/6)
2766 CCCGC
MslI 3067 CATCAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3095 CATGATCATG
NciI 1002 CCGGG CC^[GC]GG
5419 CCGGG
NsiI 3517 ATGCAT ATGCA^T
5325 ATGCAT
PflMI 664 CCAGTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5448 CCAGCACTTGG
PstI 964 CTGCAG CTGCA^G
973 CTGCAG
SacI 3805 GAGCTC GAGCT^C
4420 GAGCTC
SapI 3329 GAAGAGC GCTCTTC(1/4)
4565 GCTCTTC
ScaI 3786 AGTACT AGT^ACT
4359 AGTACT
SphI 1278 GCATGC GCATG^C
2381 GCATGC
SspI 2274 AATATT AAT^ATT
5002 AATATT
Van91I 664 CCAGTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5448 CCAGCACTTGG
XbaI 2628 TCTAGA T^CTAGA
4570 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AspI AvaII BanII BbsI BbvI
BclI BfaI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspMI BsrDI BsrI BssHII BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EarI
EcoO109I EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MvnI MwoI NcoI NdeII
NheI NlaIII NlaIV NspI PflFI
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SpeI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XcmI XhoII XmnI