##################################
# NO CUT ENZYMES (   86 enzymes) #
##################################

Acc65I      AccIII      AclI        AflIII      AgeI        
Alw44I      Aor51HI     ApaI        ApaLI       AscI        
Asp718I     AvaI        BamHI       BbeI        BbrPI       
BbvCI       BglII       BseAI       BsiEI       BsiWI       
BsmBI       BsoBI       Bsp1407I    BspEI       BspHI       
BspLU11I    BsrBI       BsrGI       BssSI       BstBI       
BstEII      BstPI       CpoI        DraIII      EagI        
EclXI       Eco47III    Eco52I      EcoO65I     FseI        
HaeII       HgaI        HincII      HindII      HpaI        
KasI        KpnI        KspI        MfeI        MluI        
MroI        MunI        NaeI        NarI        NdeI        
NgoMIV      NotI        NruI        NspV        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshAI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
Sse8387I    SspBI       SwaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AatII        2943  GACGTC               GACGT^C
AccI         5265  GTATAC               GT^[AC][GT]AC
AcyI         2943  GACGTC               G[GA]^CG[CT]C
AflII        4107  CTTAAG               C^TTAAG
AhdI         2943  GACGTCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          375  CCTAAGG              CC^T[ACGT]AGG
AseI         3289  ATTAAT               AT^TAAT
AsnI         3289  ATTAAT               AT^TAAT
AspEI        2943  GACGTCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        3970  TGCGCA               TGC^GCA
AvrII        1386  CCTAGG               C^CTAGG
BalI         3011  TGGCCA               TGG^CCA
BanI         2579  GGCACC               G^G[CT][GA]CC
BcgI         3584  CGATCATAGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1738  GTATCC               GTATCC(6/5)
BfrI         4107  CTTAAG               C^TTAAG
BglI         1938  GCCCATCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         1386  CCTAGG               C^CTAGG
BsaBI        2436  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        2943  GACGTC               G[GA]^CG[CT]C
BsaWI        2023  ACCGGA               [AT]^CCGG[AT]
BspDI        2338  ATCGAT               AT^CGAT
BsrFI        3082  GCCGGT               [GA]^CCGG[CT]
Bst1107I     5265  GTATAC               GTA^TAC
BstZ17I      5265  GTATAC               GTA^TAC
Bsu36I        375  CCTAAGG              CC^T[ACGT]AGG
Cfr10I       3082  GCCGGT               [GA]^CCGG[CT]
ClaI         2338  ATCGAT               AT^CGAT
DrdI         4376  GACAGACCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     2943  GACGTCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        375  CCTAAGG              CC^T[ACGT]AGG
EcoRI           1  GAATTC               G^AATTC
EcoRV        5291  GATATC               GAT^ATC
FspI         3970  TGCGCA               TGC^GCA
Hin1I        2943  GACGTC               G[GA]^CG[CT]C
HindIII      2530  AAGCTT               A^AGCTT
MamI         2436  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        3011  TGGCCA               TGG^CCA
MscI         3011  TGGCCA               TGG^CCA
PshBI        3289  ATTAAT               AT^TAAT
StuI          184  AGGCCT               AGG^CCT


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

BcnI         1002  CCGGG                CC^[GC]GG
             5419  CCGGG               
BlpI          153  GCTGAGC              GC^T[ACGT]AGC
             3761  GCTTAGC             
Bpu1102I      153  GCTGAGC              GC^T[ACGT]AGC
             3761  GCTTAGC             
BsaAI         685  CACGTA               [CT]AC^GT[GA]
             3861  TACGTG              
BsgI          243  GTGCAG               GTGCAG(16/14)
             1072  GTGCAG              
CelII         153  GCTGAGC              GC^T[ACGT]AGC
             3761  GCTTAGC             
EaeI         1123  TGGCCG               [CT]^GGCC[GA]
             3011  TGGCCA              
EcoNI        1337  CCTCCACAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2284  CCTATGGCAGG         
EcoT22I      3517  ATGCAT               ATGCA^T
             5325  ATGCAT              
FauI         1952  CCCGC                CCCGC(4/6)
             2766  CCCGC               
MslI         3067  CATCAAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             3095  CATGATCATG          
NciI         1002  CCGGG                CC^[GC]GG
             5419  CCGGG               
NsiI         3517  ATGCAT               ATGCA^T
             5325  ATGCAT              
PflMI         664  CCAGTAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             5448  CCAGCACTTGG         
PstI          964  CTGCAG               CTGCA^G
              973  CTGCAG              
SacI         3805  GAGCTC               GAGCT^C
             4420  GAGCTC              
SapI         3329  GAAGAGC              GCTCTTC(1/4)
             4565  GCTCTTC             
ScaI         3786  AGTACT               AGT^ACT
             4359  AGTACT              
SphI         1278  GCATGC               GCATG^C
             2381  GCATGC              
SspI         2274  AATATT               AAT^ATT
             5002  AATATT              
Van91I        664  CCAGTAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             5448  CCAGCACTTGG         
XbaI         2628  TCTAGA               T^CTAGA
             4570  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (  109 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        Asp700I     AspHI       
AspI        AvaII       BanII       BbsI        BbvI        
BclI        BfaI        BmyI        BpmI        BpuAI       
BsaI        BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        Bsp1286I    
BspMI       BsrDI       BsrI        BssHII      BssKI       
BstAPI      BstF5I      BstNI       BstUI       BstXI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraI        DraII       DsaI        EarI        
EcoO109I    EcoRII      EcoT14I     FbaI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MspA1I      MspI        
MvaI        MvnI        MwoI        NcoI        NdeII       
NheI        NlaIII      NlaIV       NspI        PflFI       
PleI        PpuMI       PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SpeI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       Tth111I     
TthHB8I     XcmI        XhoII       XmnI