################################## # NO CUT ENZYMES ( 60 enzymes) # ################################## AatII AccIII AclI AgeI AhdI AscI AspEI AviII BbeI BcgI BclI BlpI Bpu1102I BsaBI BseAI BsiWI BspDI BspEI BspHI BspLU11I BssHII Bst1107I BstBI BstZ17I CelII ClaI CpoI Eam1105I EcoRV EcoT22I FbaI FseI FspI KasI MamI MfeI MluI MroI MunI NarI NotI NruI NsiI NspV PacI PinAI PmeI Psp1406I PvuI RcaI RsrII SalI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SspI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Acc65I 4875 GGTACC G^GTACC AccII 4037 CGCG CG^CG AcyI 2316 GGCGTC G[GA]^CG[CT]C AflII 5229 CTTAAG C^TTAAG Aor51HI 4445 AGCGCT AGC^GCT ApaI 4439 GGGCCC GGGCC^C AseI 5615 ATTAAT AT^TAAT AsnI 5615 ATTAAT AT^TAAT Asp718I 4875 GGTACC G^GTACC AvrII 1674 CCTAGG C^CTAGG BalI 4023 TGGCCA TGG^CCA BamHI 4218 GGATCC G^GATCC BfrI 5229 CTTAAG C^TTAAG BlnI 1674 CCTAGG C^CTAGG BsaHI 2316 GGCGTC G[GA]^CG[CT]C BsaWI 1905 TCCGGT [AT]^CCGG[AT] BsmBI 2132 GAGACG CGTCTC(1/5) BstAPI 881 GCAGGCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 4037 CGCG CG^CG DrdI 4534 GACCTCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 4445 AGCGCT AGC^GCT Hin1I 2316 GGCGTC G[GA]^CG[CT]C HpaI 1069 GTTAAC GTT^AAC KpnI 4875 GGTACC GGTAC^C KspI 4036 CCGCGG CCGC^GG MluNI 4023 TGGCCA TGG^CCA MscI 4023 TGGCCA TGG^CCA MvnI 4037 CGCG CG^CG NaeI 5673 GCCGGC GCC^GGC NgoMIV 5673 GCCGGC G^CCGGC PaeR7I 681 CTCGAG C^TCGAG PshBI 5615 ATTAAT AT^TAAT SacII 4036 CCGCGG CCGC^GG SbfI 4911 CCTGCAGG CCTGCA^GG SphI 4484 GCATGC GCATG^C Sse8387I 4911 CCTGCAGG CCTGCA^GG SwaI 579 ATTTAAAT ATTT^AAAT XcmI 2915 CCACACCACCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 681 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AccI 116 GTCTAC GT^[AC][GT]AC 5412 GTCTAC AflIII 3145 ACGTGT A^C[GA][CT]GT 5105 ACGTGT Asp700I 1110 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2710 GAATGATTTC AspI 4367 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4889 GACAGAGTC BbrPI 3144 CACGTG CAC^GTG 5104 CACGTG BciVI 1100 GGATAC GTATCC(6/5) 2295 GGATAC BglII 199 AGATCT A^GATCT 4521 AGATCT BsaAI 3144 CACGTG [CT]AC^GT[GA] 5104 CACGTG BsiEI 828 CGGCCG CG[GA][CT]^CG 5671 CGGCCG BsrDI 5498 CATTGC GCAATG(2/0) 4574 GCAATG BsrFI 273 ACCGGC [GA]^CCGG[CT] 5673 GCCGGC BstEII 4336 GGTCACC G^GT[ACGT]ACC 4763 GGTTACC BstPI 4336 GGTCACC G^GT[ACGT]ACC 4763 GGTTACC Cfr10I 273 ACCGGC [GA]^CCGG[CT] 5673 GCCGGC DraI 580 TTTAAA TTT^AAA 5374 TTTAAA EagI 828 CGGCCG C^GGCCG 5671 CGGCCG EclXI 828 CGGCCG C^GGCCG 5671 CGGCCG Eco52I 828 CGGCCG C^GGCCG 5671 CGGCCG EcoO65I 4336 GGTCACC G^GT[ACGT]ACC 4763 GGTTACC EcoRI 1826 GAATTC G^AATTC 2210 GAATTC HaeII 82 GGCGCT [GA]GCGC^[CT] 4445 AGCGCT HindIII 1087 AAGCTT A^AGCTT 3822 AAGCTT NdeI 4880 CATATG CA^TATG 5116 CATATG NheI 2051 GCTAGC G^CTAGC 3937 GCTAGC NspI 264 GCATGT [GA]CATG^[CT] 4484 GCATGC PflFI 4367 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4889 GACAGAGTC PmaCI 3144 CACGTG CAC^GTG 5104 CACGTG PmlI 3144 CACGTG CAC^GTG 5104 CACGTG SacI 389 GAGCTC GAGCT^C 3005 GAGCTC SapI 3989 GAAGAGC GCTCTTC(1/4) 5433 GCTCTTC SexAI 2656 ACCAGGT A^CC[AT]GGT 3109 ACCTGGT Tth111I 4367 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4889 GACAGAGTC XbaI 3028 TCTAGA T^CTAGA 4298 TCTAGA XmnI 1110 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2710 GAATGATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 125 enzymes) # ######################################### AciI AcsI AfaI AluI Alw44I AlwI AlwNI AocI ApaLI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspMI BsrBI BsrGI BsrI BssKI BssSI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PflMI PleI PpuMI PshAI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmaCI XmaI