##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
AscI AspEI AviII BbeI BcgI
BclI BlpI Bpu1102I BsaBI BseAI
BsiWI BspDI BspEI BspHI BspLU11I
BssHII Bst1107I BstBI BstZ17I CelII
ClaI CpoI Eam1105I EcoRV EcoT22I
FbaI FseI FspI KasI MamI
MfeI MluI MroI MunI NarI
NotI NruI NsiI NspV PacI
PinAI PmeI Psp1406I PvuI RcaI
RsrII SalI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI SspI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 4875 GGTACC G^GTACC
AccII 4037 CGCG CG^CG
AcyI 2316 GGCGTC G[GA]^CG[CT]C
AflII 5229 CTTAAG C^TTAAG
Aor51HI 4445 AGCGCT AGC^GCT
ApaI 4439 GGGCCC GGGCC^C
AseI 5615 ATTAAT AT^TAAT
AsnI 5615 ATTAAT AT^TAAT
Asp718I 4875 GGTACC G^GTACC
AvrII 1674 CCTAGG C^CTAGG
BalI 4023 TGGCCA TGG^CCA
BamHI 4218 GGATCC G^GATCC
BfrI 5229 CTTAAG C^TTAAG
BlnI 1674 CCTAGG C^CTAGG
BsaHI 2316 GGCGTC G[GA]^CG[CT]C
BsaWI 1905 TCCGGT [AT]^CCGG[AT]
BsmBI 2132 GAGACG CGTCTC(1/5)
BstAPI 881 GCAGGCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 4037 CGCG CG^CG
DrdI 4534 GACCTCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4445 AGCGCT AGC^GCT
Hin1I 2316 GGCGTC G[GA]^CG[CT]C
HpaI 1069 GTTAAC GTT^AAC
KpnI 4875 GGTACC GGTAC^C
KspI 4036 CCGCGG CCGC^GG
MluNI 4023 TGGCCA TGG^CCA
MscI 4023 TGGCCA TGG^CCA
MvnI 4037 CGCG CG^CG
NaeI 5673 GCCGGC GCC^GGC
NgoMIV 5673 GCCGGC G^CCGGC
PaeR7I 681 CTCGAG C^TCGAG
PshBI 5615 ATTAAT AT^TAAT
SacII 4036 CCGCGG CCGC^GG
SbfI 4911 CCTGCAGG CCTGCA^GG
SphI 4484 GCATGC GCATG^C
Sse8387I 4911 CCTGCAGG CCTGCA^GG
SwaI 579 ATTTAAAT ATTT^AAAT
XcmI 2915 CCACACCACCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 681 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 116 GTCTAC GT^[AC][GT]AC
5412 GTCTAC
AflIII 3145 ACGTGT A^C[GA][CT]GT
5105 ACGTGT
Asp700I 1110 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2710 GAATGATTTC
AspI 4367 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4889 GACAGAGTC
BbrPI 3144 CACGTG CAC^GTG
5104 CACGTG
BciVI 1100 GGATAC GTATCC(6/5)
2295 GGATAC
BglII 199 AGATCT A^GATCT
4521 AGATCT
BsaAI 3144 CACGTG [CT]AC^GT[GA]
5104 CACGTG
BsiEI 828 CGGCCG CG[GA][CT]^CG
5671 CGGCCG
BsrDI 5498 CATTGC GCAATG(2/0)
4574 GCAATG
BsrFI 273 ACCGGC [GA]^CCGG[CT]
5673 GCCGGC
BstEII 4336 GGTCACC G^GT[ACGT]ACC
4763 GGTTACC
BstPI 4336 GGTCACC G^GT[ACGT]ACC
4763 GGTTACC
Cfr10I 273 ACCGGC [GA]^CCGG[CT]
5673 GCCGGC
DraI 580 TTTAAA TTT^AAA
5374 TTTAAA
EagI 828 CGGCCG C^GGCCG
5671 CGGCCG
EclXI 828 CGGCCG C^GGCCG
5671 CGGCCG
Eco52I 828 CGGCCG C^GGCCG
5671 CGGCCG
EcoO65I 4336 GGTCACC G^GT[ACGT]ACC
4763 GGTTACC
EcoRI 1826 GAATTC G^AATTC
2210 GAATTC
HaeII 82 GGCGCT [GA]GCGC^[CT]
4445 AGCGCT
HindIII 1087 AAGCTT A^AGCTT
3822 AAGCTT
NdeI 4880 CATATG CA^TATG
5116 CATATG
NheI 2051 GCTAGC G^CTAGC
3937 GCTAGC
NspI 264 GCATGT [GA]CATG^[CT]
4484 GCATGC
PflFI 4367 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4889 GACAGAGTC
PmaCI 3144 CACGTG CAC^GTG
5104 CACGTG
PmlI 3144 CACGTG CAC^GTG
5104 CACGTG
SacI 389 GAGCTC GAGCT^C
3005 GAGCTC
SapI 3989 GAAGAGC GCTCTTC(1/4)
5433 GCTCTTC
SexAI 2656 ACCAGGT A^CC[AT]GGT
3109 ACCTGGT
Tth111I 4367 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4889 GACAGAGTC
XbaI 3028 TCTAGA T^CTAGA
4298 TCTAGA
XmnI 1110 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2710 GAATGATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI AlwNI AocI ApaLI ApoI
AspHI AvaI AvaII BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspMI BsrBI BsrGI
BsrI BssKI BssSI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV PflMI PleI PpuMI PshAI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
SspBI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmaCI XmaI