################################## # NO CUT ENZYMES ( 64 enzymes) # ################################## AatII AccII AccIII AclI AgeI AscI AseI AsnI AspI BbrPI BglII BsaWI BseAI BsiEI BsiWI BsmBI BspEI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I CpoI EagI EclXI Eco52I EcoO65I EcoRV FseI KspI MfeI MluI MroI MunI MvnI NcoI NotI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI ScaI SfiI SfuI SgrAI SnaBI SpeI SspI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AcsI 32 GAATTC [GA]^AATT[CT] AflII 1449 CTTAAG C^TTAAG AhdI 1641 GACTGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 32 GAATTC [GA]^AATT[CT] Asp700I 3774 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1641 GACTGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 2305 GGATCC G^GATCC BcgI 4621 CGAGTTAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1670 TGATCA T^GATCA BfrI 1449 CTTAAG C^TTAAG BglI 1488 GCCGATCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 1607 CACGTA [CT]AC^GT[GA] BsaBI 2547 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmI 3 GCATTC GAATGC(1/-1) BspDI 2197 ATCGAT AT^CGAT BspHI 1979 TCATGA T^CATGA ClaI 2197 ATCGAT AT^CGAT DraI 4784 TTTAAA TTT^AAA DraIII 1561 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 427 GACAAGTTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 1641 GACTGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 32 GAATTC G^AATTC EcoT22I 2376 ATGCAT ATGCA^T FbaI 1670 TGATCA T^GATCA HpaI 2739 GTTAAC GTT^AAC MamI 2547 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NdeI 3201 CATATG CA^TATG NheI 636 GCTAGC G^CTAGC NsiI 2376 ATGCAT ATGCA^T PaeR7I 332 CTCGAG C^TCGAG PshAI 2763 GACAGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 1979 TCATGA T^CATGA SacI 923 GAGCTC GAGCT^C SbfI 745 CCTGCAGG CCTGCA^GG SmaI 3604 CCCGGG CCC^GGG SphI 2374 GCATGC GCATG^C Sse8387I 745 CCTGCAGG CCTGCA^GG StuI 1289 AGGCCT AGG^CCT Tsp509I 33 AATT ^AATT XhoI 332 CTCGAG C^TCGAG XmaCI 3604 CCCGGG C^CCGGG XmaI 3604 CCCGGG C^CCGGG XmnI 3774 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### Aor51HI 1631 AGCGCT AGC^GCT 2003 AGCGCT AviII 3160 TGCGCA TGC^GCA 4719 TGCGCA AvrII 3007 CCTAGG C^CTAGG 4588 CCTAGG BbeI 674 GGCGCC GGCGC^C 2810 GGCGCC BbvCI 1615 CCTCAGC CCTCAGC(-5/-2) 1105 GCTGAGG BlnI 3007 CCTAGG C^CTAGG 4588 CCTAGG BspLU11I 2753 ACATGT A^CATGT 2985 ACATGT BspMI 4527 ACCTGC ACCTGC(4/8) 3263 GCAGGT BstAPI 553 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3572 GCACCCACTGC Eco47III 1631 AGCGCT AGC^GCT 2003 AGCGCT FspI 3160 TGCGCA TGC^GCA 4719 TGCGCA HindIII 76 AAGCTT A^AGCTT 271 AAGCTT KasI 674 GGCGCC G^GCGCC 2810 GGCGCC MaeII 1608 ACGT A^CGT 4391 ACGT NaeI 1241 GCCGGC GCC^GGC 3118 GCCGGC NarI 674 GGCGCC GG^CGCC 2810 GGCGCC NgoMIV 1241 GCCGGC G^CCGGC 3118 GCCGGC SfoI 674 GGCGCC GGC^GCC 2810 GGCGCC ######################################### # 3 OR MORE CUT ENZYMES ( 133 enzymes) # ######################################### Acc65I AccI AciI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI AocI ApaI ApaLI Asp718I AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BciVI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BsrBI BsrDI BsrFI BsrGI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoII