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# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AccII AccIII AclI AgeI
AscI AseI AsnI AspI BbrPI
BglII BsaWI BseAI BsiEI BsiWI
BsmBI BspEI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
CpoI EagI EclXI Eco52I EcoO65I
EcoRV FseI KspI MfeI MluI
MroI MunI MvnI NcoI NotI
NruI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SalI ScaI
SfiI SfuI SgrAI SnaBI SpeI
SspI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AcsI 32 GAATTC [GA]^AATT[CT]
AflII 1449 CTTAAG C^TTAAG
AhdI 1641 GACTGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 32 GAATTC [GA]^AATT[CT]
Asp700I 3774 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1641 GACTGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 2305 GGATCC G^GATCC
BcgI 4621 CGAGTTAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1670 TGATCA T^GATCA
BfrI 1449 CTTAAG C^TTAAG
BglI 1488 GCCGATCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1607 CACGTA [CT]AC^GT[GA]
BsaBI 2547 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 3 GCATTC GAATGC(1/-1)
BspDI 2197 ATCGAT AT^CGAT
BspHI 1979 TCATGA T^CATGA
ClaI 2197 ATCGAT AT^CGAT
DraI 4784 TTTAAA TTT^AAA
DraIII 1561 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 427 GACAAGTTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1641 GACTGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 32 GAATTC G^AATTC
EcoT22I 2376 ATGCAT ATGCA^T
FbaI 1670 TGATCA T^GATCA
HpaI 2739 GTTAAC GTT^AAC
MamI 2547 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 3201 CATATG CA^TATG
NheI 636 GCTAGC G^CTAGC
NsiI 2376 ATGCAT ATGCA^T
PaeR7I 332 CTCGAG C^TCGAG
PshAI 2763 GACAGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1979 TCATGA T^CATGA
SacI 923 GAGCTC GAGCT^C
SbfI 745 CCTGCAGG CCTGCA^GG
SmaI 3604 CCCGGG CCC^GGG
SphI 2374 GCATGC GCATG^C
Sse8387I 745 CCTGCAGG CCTGCA^GG
StuI 1289 AGGCCT AGG^CCT
Tsp509I 33 AATT ^AATT
XhoI 332 CTCGAG C^TCGAG
XmaCI 3604 CCCGGG C^CCGGG
XmaI 3604 CCCGGG C^CCGGG
XmnI 3774 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Aor51HI 1631 AGCGCT AGC^GCT
2003 AGCGCT
AviII 3160 TGCGCA TGC^GCA
4719 TGCGCA
AvrII 3007 CCTAGG C^CTAGG
4588 CCTAGG
BbeI 674 GGCGCC GGCGC^C
2810 GGCGCC
BbvCI 1615 CCTCAGC CCTCAGC(-5/-2)
1105 GCTGAGG
BlnI 3007 CCTAGG C^CTAGG
4588 CCTAGG
BspLU11I 2753 ACATGT A^CATGT
2985 ACATGT
BspMI 4527 ACCTGC ACCTGC(4/8)
3263 GCAGGT
BstAPI 553 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3572 GCACCCACTGC
Eco47III 1631 AGCGCT AGC^GCT
2003 AGCGCT
FspI 3160 TGCGCA TGC^GCA
4719 TGCGCA
HindIII 76 AAGCTT A^AGCTT
271 AAGCTT
KasI 674 GGCGCC G^GCGCC
2810 GGCGCC
MaeII 1608 ACGT A^CGT
4391 ACGT
NaeI 1241 GCCGGC GCC^GGC
3118 GCCGGC
NarI 674 GGCGCC GG^CGCC
2810 GGCGCC
NgoMIV 1241 GCCGGC G^CCGGC
3118 GCCGGC
SfoI 674 GGCGCC GGC^GCC
2810 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
Acc65I AccI AciI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI Asp718I AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BciVI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BsrBI BsrDI
BsrFI BsrGI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KpnI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I TspRI TthHB8I
Van91I XcmI XhoII