##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII Acc65I AccII AflII AgeI
Alw44I ApaLI AscI AseI AsnI
Asp718I AviII BfrI BsiEI BsiWI
BspDI BspHI BsrBI BssHII Bst1107I
BstUI BstZ17I ClaI DrdI EagI
EclXI Eco52I FseI FspI HindIII
HpaI KpnI KspI MluI MvnI
NdeI NotI NruI PacI PinAI
PmeI PshBI PvuI RcaI SacII
SapI SbfI SfiI SgrAI SnaBI
SpeI Sse8387I SwaI XbaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 482 TCCGGA T^CCGGA
AclI 903 AACGTT AA^CGTT
AhdI 4146 GACTCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 4146 GACTCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2995 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3258 CCTAGG C^CTAGG
BamHI 2372 GGATCC G^GATCC
BbeI 4457 GGCGCC GGCGC^C
BbvCI 4654 CCTCAGC CCTCAGC(-5/-2)
BciVI 2965 GGATAC GTATCC(6/5)
BlnI 3258 CCTAGG C^CTAGG
BsaBI 91 GATTGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 464 GAGACC GGTCTC(1/5)
BseAI 482 TCCGGA T^CCGGA
Bsp1407I 171 TGTACA T^GTACA
BspEI 482 TCCGGA T^CCGGA
BsrGI 171 TGTACA T^GTACA
CpoI 4180 CGGACCG CG^G[AT]CCG
DraI 4241 TTTAAA TTT^AAA
Eam1105I 4146 GACTCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1546 CCTGGATTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1560 GATATC GAT^ATC
EcoT22I 4219 ATGCAT ATGCA^T
KasI 4457 GGCGCC G^GCGCC
MamI 91 GATTGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4232 CAATTG C^AATTG
MroI 482 TCCGGA T^CCGGA
MunI 4232 CAATTG C^AATTG
NarI 4457 GGCGCC GG^CGCC
NheI 4486 GCTAGC G^CTAGC
NsiI 4219 ATGCAT ATGCA^T
PaeR7I 4618 CTCGAG C^TCGAG
PflFI 2995 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 903 AACGTT AA^CGTT
RsrII 4180 CGGACCG CG^G[AT]CCG
SalI 4157 GTCGAC G^TCGAC
SexAI 4324 ACCAGGT A^CC[AT]GGT
SfoI 4457 GGCGCC GGC^GCC
SspBI 171 TGTACA T^GTACA
Tth111I 2995 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 4618 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 3433 GTAGAC GT^[AC][GT]AC
4157 GTCGAC
AcyI 404 GACGCC G[GA]^CG[CT]C
4457 GGCGCC
AocI 2498 CCTTAGG CC^T[ACGT]AGG
2822 CCTTAGG
Aor51HI 861 AGCGCT AGC^GCT
2320 AGCGCT
ApaI 64 GGGCCC GGGCC^C
3751 GGGCCC
BcgI 446 GCACCCGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
765 GCACACTGGTCG
BclI 270 TGATCA T^GATCA
648 TGATCA
BlpI 3115 GCTGAGC GC^T[ACGT]AGC
4736 GCTAAGC
Bpu1102I 3115 GCTGAGC GC^T[ACGT]AGC
4736 GCTAAGC
BsaHI 404 GACGCC G[GA]^CG[CT]C
4457 GGCGCC
BsaWI 482 TCCGGA [AT]^CCGG[AT]
3456 ACCGGA
BsmBI 2120 CGTCTC CGTCTC(1/5)
4016 CGTCTC
BspLU11I 1858 ACATGT A^CATGT
2282 ACATGT
BssSI 21 CACGAG CACGAG(-5/-1)
1375 CACGAG
Bsu36I 2498 CCTTAGG CC^T[ACGT]AGG
2822 CCTTAGG
CelII 3115 GCTGAGC GC^T[ACGT]AGC
4736 GCTAAGC
DraIII 1163 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
1881 CACACAGTG
Eco47III 861 AGCGCT AGC^GCT
2320 AGCGCT
Eco81I 2498 CCTTAGG CC^T[ACGT]AGG
2822 CCTTAGG
FbaI 270 TGATCA T^GATCA
648 TGATCA
HgaI 404 GACGC GACGC(5/10)
517 GACGC
Hin1I 404 GACGCC G[GA]^CG[CT]C
4457 GGCGCC
HincII 1839 GTTGAC GT[CT]^[GA]AC
4157 GTCGAC
HindII 1839 GTTGAC GT[CT]^[GA]AC
4157 GTCGAC
NaeI 3760 GCCGGC GCC^GGC
4381 GCCGGC
NcoI 507 CCATGG C^CATGG
3162 CCATGG
NgoMIV 3760 GCCGGC G^CCGGC
4381 GCCGGC
PflMI 948 CCAGGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1247 CCACTGGATGG
PshAI 3366 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4010 GACTGTCGTC
PvuII 1040 CAGCTG CAG^CTG
4657 CAGCTG
ScaI 285 AGTACT AGT^ACT
2776 AGTACT
SmaI 2598 CCCGGG CCC^GGG
3747 CCCGGG
StuI 1350 AGGCCT AGG^CCT
2659 AGGCCT
Van91I 948 CCAGGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1247 CCACTGGATGG
XmaCI 2598 CCCGGG C^CCGGG
3747 CCCGGG
XmaI 2598 CCCGGG C^CCGGG
3747 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbrPI BbsI BbvI BcnI BfaI
BglI BglII BmyI BpmI BpuAI
BsaAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BstAPI BstBI BstEII
BstF5I BstNI BstPI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoO65I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI NspV PleI
PmaCI PmlI PpuMI PstI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SfuI SmlI SphI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI