################################## # NO CUT ENZYMES ( 54 enzymes) # ################################## AatII Acc65I AccII AflII AgeI Alw44I ApaLI AscI AseI AsnI Asp718I AviII BfrI BsiEI BsiWI BspDI BspHI BsrBI BssHII Bst1107I BstUI BstZ17I ClaI DrdI EagI EclXI Eco52I FseI FspI HindIII HpaI KpnI KspI MluI MvnI NdeI NotI NruI PacI PinAI PmeI PshBI PvuI RcaI SacII SapI SbfI SfiI SgrAI SnaBI SpeI Sse8387I SwaI XbaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccIII 482 TCCGGA T^CCGGA AclI 903 AACGTT AA^CGTT AhdI 4146 GACTCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 4146 GACTCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2995 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 3258 CCTAGG C^CTAGG BamHI 2372 GGATCC G^GATCC BbeI 4457 GGCGCC GGCGC^C BbvCI 4654 CCTCAGC CCTCAGC(-5/-2) BciVI 2965 GGATAC GTATCC(6/5) BlnI 3258 CCTAGG C^CTAGG BsaBI 91 GATTGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 464 GAGACC GGTCTC(1/5) BseAI 482 TCCGGA T^CCGGA Bsp1407I 171 TGTACA T^GTACA BspEI 482 TCCGGA T^CCGGA BsrGI 171 TGTACA T^GTACA CpoI 4180 CGGACCG CG^G[AT]CCG DraI 4241 TTTAAA TTT^AAA Eam1105I 4146 GACTCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 1546 CCTGGATTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 1560 GATATC GAT^ATC EcoT22I 4219 ATGCAT ATGCA^T KasI 4457 GGCGCC G^GCGCC MamI 91 GATTGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 4232 CAATTG C^AATTG MroI 482 TCCGGA T^CCGGA MunI 4232 CAATTG C^AATTG NarI 4457 GGCGCC GG^CGCC NheI 4486 GCTAGC G^CTAGC NsiI 4219 ATGCAT ATGCA^T PaeR7I 4618 CTCGAG C^TCGAG PflFI 2995 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC Psp1406I 903 AACGTT AA^CGTT RsrII 4180 CGGACCG CG^G[AT]CCG SalI 4157 GTCGAC G^TCGAC SexAI 4324 ACCAGGT A^CC[AT]GGT SfoI 4457 GGCGCC GGC^GCC SspBI 171 TGTACA T^GTACA Tth111I 2995 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 4618 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AccI 3433 GTAGAC GT^[AC][GT]AC 4157 GTCGAC AcyI 404 GACGCC G[GA]^CG[CT]C 4457 GGCGCC AocI 2498 CCTTAGG CC^T[ACGT]AGG 2822 CCTTAGG Aor51HI 861 AGCGCT AGC^GCT 2320 AGCGCT ApaI 64 GGGCCC GGGCC^C 3751 GGGCCC BcgI 446 GCACCCGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 765 GCACACTGGTCG BclI 270 TGATCA T^GATCA 648 TGATCA BlpI 3115 GCTGAGC GC^T[ACGT]AGC 4736 GCTAAGC Bpu1102I 3115 GCTGAGC GC^T[ACGT]AGC 4736 GCTAAGC BsaHI 404 GACGCC G[GA]^CG[CT]C 4457 GGCGCC BsaWI 482 TCCGGA [AT]^CCGG[AT] 3456 ACCGGA BsmBI 2120 CGTCTC CGTCTC(1/5) 4016 CGTCTC BspLU11I 1858 ACATGT A^CATGT 2282 ACATGT BssSI 21 CACGAG CACGAG(-5/-1) 1375 CACGAG Bsu36I 2498 CCTTAGG CC^T[ACGT]AGG 2822 CCTTAGG CelII 3115 GCTGAGC GC^T[ACGT]AGC 4736 GCTAAGC DraIII 1163 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG 1881 CACACAGTG Eco47III 861 AGCGCT AGC^GCT 2320 AGCGCT Eco81I 2498 CCTTAGG CC^T[ACGT]AGG 2822 CCTTAGG FbaI 270 TGATCA T^GATCA 648 TGATCA HgaI 404 GACGC GACGC(5/10) 517 GACGC Hin1I 404 GACGCC G[GA]^CG[CT]C 4457 GGCGCC HincII 1839 GTTGAC GT[CT]^[GA]AC 4157 GTCGAC HindII 1839 GTTGAC GT[CT]^[GA]AC 4157 GTCGAC NaeI 3760 GCCGGC GCC^GGC 4381 GCCGGC NcoI 507 CCATGG C^CATGG 3162 CCATGG NgoMIV 3760 GCCGGC G^CCGGC 4381 GCCGGC PflMI 948 CCAGGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1247 CCACTGGATGG PshAI 3366 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 4010 GACTGTCGTC PvuII 1040 CAGCTG CAG^CTG 4657 CAGCTG ScaI 285 AGTACT AGT^ACT 2776 AGTACT SmaI 2598 CCCGGG CCC^GGG 3747 CCCGGG StuI 1350 AGGCCT AGG^CCT 2659 AGGCCT Van91I 948 CCAGGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1247 CCACTGGATGG XmaCI 2598 CCCGGG C^CCGGG 3747 CCCGGG XmaI 2598 CCCGGG C^CCGGG 3747 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 127 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AvaI AvaII BalI BanI BanII BbrPI BbsI BbvI BcnI BfaI BglI BglII BmyI BpmI BpuAI BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstAPI BstBI BstEII BstF5I BstNI BstPI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoO65I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI NspV PleI PmaCI PmlI PpuMI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfuI SmlI SphI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI