##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflIII AgeI Alw44I ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
BamHI BbrPI BclI BglII BsaAI
BsaBI BseAI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI Bst1107I BstZ17I
ClaI CpoI DraI DraIII EcoRI
EcoRV EcoT22I FbaI FseI HpaI
KpnI MamI MfeI MluI MroI
MunI NdeI NheI NotI NsiI
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SfiI SgrAI SnaBI
SphI SspBI SspI SwaI XbaI
XcmI XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AccI 2916 GTAGAC GT^[AC][GT]AC
AflII 3886 CTTAAG C^TTAAG
AhdI 5651 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 5651 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3657 CCTAGG C^CTAGG
BcgI 4304 GCAGCCGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 5269 GTATCC GTATCC(6/5)
BfrI 3886 CTTAAG C^TTAAG
BlnI 3657 CCTAGG C^CTAGG
BsiEI 3548 CGGCCG CG[GA][CT]^CG
BsiWI 488 CGTACG C^GTACG
BsmBI 5322 CGTCTC CGTCTC(1/5)
BsrDI 3783 CATTGC GCAATG(2/0)
BsrFI 2137 GCCGGC [GA]^CCGG[CT]
BstBI 4973 TTCGAA TT^CGAA
Cfr10I 2137 GCCGGC [GA]^CCGG[CT]
DrdI 2048 GACGGCGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3548 CGGCCG C^GGCCG
Eam1105I 5651 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3548 CGGCCG C^GGCCG
Eco52I 3548 CGGCCG C^GGCCG
KspI 324 CCGCGG CCGC^GG
NaeI 2137 GCCGGC GCC^GGC
NcoI 4784 CCATGG C^CATGG
NgoMIV 2137 GCCGGC G^CCGGC
NruI 3143 TCGCGA TCG^CGA
NspV 4973 TTCGAA TT^CGAA
SacII 324 CCGCGG CCGC^GG
ScaI 2894 AGTACT AGT^ACT
SfuI 4973 TTCGAA TT^CGAA
SpeI 3307 ACTAGT A^CTAGT
Tsp509I 1096 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AocI 194 CCTGAGG CC^T[ACGT]AGG
4935 CCTCAGG
Aor51HI 856 AGCGCT AGC^GCT
4898 AGCGCT
AspI 87 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1514 GACCCTGTC
BsaWI 2152 ACCGGA [AT]^CCGG[AT]
5124 TCCGGT
BsmI 5001 GAATGC GAATGC(1/-1)
329 GCATTC
BssHII 1430 GCGCGC G^CGCGC
4278 GCGCGC
BssSI 2997 CACGAG CACGAG(-5/-1)
5671 CTCGTG
BstEII 674 GGTGACC G^GT[ACGT]ACC
3195 GGTAACC
BstPI 674 GGTGACC G^GT[ACGT]ACC
3195 GGTAACC
Bsu36I 194 CCTGAGG CC^T[ACGT]AGG
4935 CCTCAGG
Eco47III 856 AGCGCT AGC^GCT
4898 AGCGCT
Eco81I 194 CCTGAGG CC^T[ACGT]AGG
4935 CCTCAGG
EcoO65I 674 GGTGACC G^GT[ACGT]ACC
3195 GGTAACC
HincII 93 GTCAAC GT[CT]^[GA]AC
5714 GTTGAC
HindII 93 GTCAAC GT[CT]^[GA]AC
5714 GTTGAC
HindIII 2481 AAGCTT A^AGCTT
4969 AAGCTT
MseI 1098 TTAA T^TAA
3887 TTAA
MslI 1172 CACTACGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
5430 CATCAGAGTG
NspI 445 ACATGC [GA]CATG^[CT]
1166 ACATGC
PflFI 87 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1514 GACCCTGTC
SapI 2241 GAAGAGC GCTCTTC(1/4)
5518 GAAGAGC
SexAI 88 ACCAGGT A^CC[AT]GGT
3084 ACCAGGT
Tru9I 1098 TTAA T^TAA
3887 TTAA
Tth111I 87 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1514 GACCCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII AviII BalI BanI BanII
BbeI BbsI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV PaeR7I
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I SbfI
ScrFI SfaNI SfcI SfoI SmaI
SmlI Sse8387I StuI StyI TaqI
TfiI TseI Tsp45I TspRI TthHB8I
Van91I XhoI XhoII XmaCI XmaI