##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AccIII AclI AgeI ApaI AscI
AviII BbeI BbrPI BbvCI BcgI
BsaBI BseAI BsiEI BsiWI BspDI
BspEI BsrBI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DrdI EagI EclXI Eco52I EcoO65I
EcoRI EcoT22I FseI FspI HpaI
KasI KspI MamI MluI MroI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
StuI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AatII 631 GACGTC GACGT^C
Acc65I 4574 GGTACC G^GTACC
AcyI 631 GACGTC G[GA]^CG[CT]C
AflII 3183 CTTAAG C^TTAAG
AflIII 1112 ACATGT A^C[GA][CT]GT
AhdI 1126 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2620 GTGCAC G^TGCAC
AocI 2856 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2526 AGCGCT AGC^GCT
ApaLI 2620 GTGCAC G^TGCAC
Asp718I 4574 GGTACC G^GTACC
AspEI 1126 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 110 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4190 CCTAGG C^CTAGG
BalI 1633 TGGCCA TGG^CCA
BamHI 2140 GGATCC G^GATCC
BclI 1597 TGATCA T^GATCA
BfrI 3183 CTTAAG C^TTAAG
BlnI 4190 CCTAGG C^CTAGG
BlpI 9 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 9 GCTGAGC GC^T[ACGT]AGC
BsaAI 288 TACGTG [CT]AC^GT[GA]
BsaHI 631 GACGTC G[GA]^CG[CT]C
BsaWI 1554 ACCGGA [AT]^CCGG[AT]
BsmBI 793 GAGACG CGTCTC(1/5)
Bsp1407I 474 TGTACA T^GTACA
BspHI 2065 TCATGA T^CATGA
BspLU11I 1112 ACATGT A^CATGT
BsrGI 474 TGTACA T^GTACA
BstAPI 150 GCATTCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2856 CCTGAGG CC^T[ACGT]AGG
CelII 9 GCTGAGC GC^T[ACGT]AGC
DraII 1834 AGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 1043 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1126 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2526 AGCGCT AGC^GCT
Eco81I 2856 CCTGAGG CC^T[ACGT]AGG
EcoO109I 1834 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRV 1375 GATATC GAT^ATC
FbaI 1597 TGATCA T^GATCA
Hin1I 631 GACGTC G[GA]^CG[CT]C
KpnI 4574 GGTACC GGTAC^C
MfeI 681 CAATTG C^AATTG
MluNI 1633 TGGCCA TGG^CCA
MscI 1633 TGGCCA TGG^CCA
MunI 681 CAATTG C^AATTG
NdeI 3331 CATATG CA^TATG
PaeR7I 2576 CTCGAG C^TCGAG
PflFI 110 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1834 AGGTCCC [GA]G^G[AT]CC[CT]
RcaI 2065 TCATGA T^CATGA
SapI 559 GAAGAGC GCTCTTC(1/4)
SmaI 4639 CCCGGG CCC^GGG
SspBI 474 TGTACA T^GTACA
Tth111I 110 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1636 CCAGAATGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2576 CTCGAG C^TCGAG
XmaCI 4639 CCCGGG C^CCGGG
XmaI 4639 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 879 CGCG CG^CG
1608 CGCG
AseI 2962 ATTAAT AT^TAAT
4716 ATTAAT
AsnI 2962 ATTAAT AT^TAAT
4716 ATTAAT
Asp700I 2447 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4341 GAAACTCTTC
BanI 1208 GGTGCC G^G[CT][GA]CC
4574 GGTACC
BbsI 1365 GTCTTC GAAGAC(2/6)
3413 GTCTTC
BglI 2228 GCCAGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4189 GCCTAGGAGGC
BglII 651 AGATCT A^GATCT
2580 AGATCT
BpuAI 1365 GTCTTC GAAGAC(2/6)
3413 GTCTTC
BsaI 2513 GGTCTC GGTCTC(1/5)
4643 GGTCTC
BsrFI 14 GCCGGT [GA]^CCGG[CT]
1024 GCCGGT
BssSI 1336 CTCGTG CACGAG(-5/-1)
1562 CTCGTG
BstUI 879 CGCG CG^CG
1608 CGCG
BstXI 1020 CCATGCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1726 CCAGGTGAGTGG
Cfr10I 14 GCCGGT [GA]^CCGG[CT]
1024 GCCGGT
DsaI 1212 CCATGG C^C[GA][CT]GG
1491 CCATGG
EaeI 1105 TGGCCG [CT]^GGCC[GA]
1633 TGGCCA
FauI 864 CCCGC CCCGC(4/6)
1146 CCCGC
HgaI 1606 GACGC GACGC(5/10)
1567 GCGTC
HincII 811 GTCAAC GT[CT]^[GA]AC
1627 GTTGAC
HindII 811 GTCAAC GT[CT]^[GA]AC
1627 GTTGAC
HindIII 1511 AAGCTT A^AGCTT
1931 AAGCTT
MvnI 879 CGCG CG^CG
1608 CGCG
NcoI 1212 CCATGG C^CATGG
1491 CCATGG
PshBI 2962 ATTAAT AT^TAAT
4716 ATTAAT
SspI 2783 AATATT AAT^ATT
4538 AATATT
TaqI 2577 TCGA T^CGA
2910 TCGA
TthHB8I 2577 TCGA T^CGA
2910 TCGA
XmnI 2447 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4341 GAAACTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BanII BbvI BciVI BcnI
BfaI BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI EarI EcoNI EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I XhoII