################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## AccIII AclI AgeI ApaI AscI AviII BbeI BbrPI BbvCI BcgI BsaBI BseAI BsiEI BsiWI BspDI BspEI BsrBI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DrdI EagI EclXI Eco52I EcoO65I EcoRI EcoT22I FseI FspI HpaI KasI KspI MamI MluI MroI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I StuI SwaI XbaI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AatII 631 GACGTC GACGT^C Acc65I 4574 GGTACC G^GTACC AcyI 631 GACGTC G[GA]^CG[CT]C AflII 3183 CTTAAG C^TTAAG AflIII 1112 ACATGT A^C[GA][CT]GT AhdI 1126 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2620 GTGCAC G^TGCAC AocI 2856 CCTGAGG CC^T[ACGT]AGG Aor51HI 2526 AGCGCT AGC^GCT ApaLI 2620 GTGCAC G^TGCAC Asp718I 4574 GGTACC G^GTACC AspEI 1126 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 110 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 4190 CCTAGG C^CTAGG BalI 1633 TGGCCA TGG^CCA BamHI 2140 GGATCC G^GATCC BclI 1597 TGATCA T^GATCA BfrI 3183 CTTAAG C^TTAAG BlnI 4190 CCTAGG C^CTAGG BlpI 9 GCTGAGC GC^T[ACGT]AGC Bpu1102I 9 GCTGAGC GC^T[ACGT]AGC BsaAI 288 TACGTG [CT]AC^GT[GA] BsaHI 631 GACGTC G[GA]^CG[CT]C BsaWI 1554 ACCGGA [AT]^CCGG[AT] BsmBI 793 GAGACG CGTCTC(1/5) Bsp1407I 474 TGTACA T^GTACA BspHI 2065 TCATGA T^CATGA BspLU11I 1112 ACATGT A^CATGT BsrGI 474 TGTACA T^GTACA BstAPI 150 GCATTCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 2856 CCTGAGG CC^T[ACGT]AGG CelII 9 GCTGAGC GC^T[ACGT]AGC DraII 1834 AGGTCCC [GA]G^G[ACGT]CC[CT] DraIII 1043 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 1126 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2526 AGCGCT AGC^GCT Eco81I 2856 CCTGAGG CC^T[ACGT]AGG EcoO109I 1834 AGGTCCC [GA]G^G[ACGT]CC[CT] EcoRV 1375 GATATC GAT^ATC FbaI 1597 TGATCA T^GATCA Hin1I 631 GACGTC G[GA]^CG[CT]C KpnI 4574 GGTACC GGTAC^C MfeI 681 CAATTG C^AATTG MluNI 1633 TGGCCA TGG^CCA MscI 1633 TGGCCA TGG^CCA MunI 681 CAATTG C^AATTG NdeI 3331 CATATG CA^TATG PaeR7I 2576 CTCGAG C^TCGAG PflFI 110 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 1834 AGGTCCC [GA]G^G[AT]CC[CT] RcaI 2065 TCATGA T^CATGA SapI 559 GAAGAGC GCTCTTC(1/4) SmaI 4639 CCCGGG CCC^GGG SspBI 474 TGTACA T^GTACA Tth111I 110 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1636 CCAGAATGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 2576 CTCGAG C^TCGAG XmaCI 4639 CCCGGG C^CCGGG XmaI 4639 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccII 879 CGCG CG^CG 1608 CGCG AseI 2962 ATTAAT AT^TAAT 4716 ATTAAT AsnI 2962 ATTAAT AT^TAAT 4716 ATTAAT Asp700I 2447 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4341 GAAACTCTTC BanI 1208 GGTGCC G^G[CT][GA]CC 4574 GGTACC BbsI 1365 GTCTTC GAAGAC(2/6) 3413 GTCTTC BglI 2228 GCCAGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 4189 GCCTAGGAGGC BglII 651 AGATCT A^GATCT 2580 AGATCT BpuAI 1365 GTCTTC GAAGAC(2/6) 3413 GTCTTC BsaI 2513 GGTCTC GGTCTC(1/5) 4643 GGTCTC BsrFI 14 GCCGGT [GA]^CCGG[CT] 1024 GCCGGT BssSI 1336 CTCGTG CACGAG(-5/-1) 1562 CTCGTG BstUI 879 CGCG CG^CG 1608 CGCG BstXI 1020 CCATGCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1726 CCAGGTGAGTGG Cfr10I 14 GCCGGT [GA]^CCGG[CT] 1024 GCCGGT DsaI 1212 CCATGG C^C[GA][CT]GG 1491 CCATGG EaeI 1105 TGGCCG [CT]^GGCC[GA] 1633 TGGCCA FauI 864 CCCGC CCCGC(4/6) 1146 CCCGC HgaI 1606 GACGC GACGC(5/10) 1567 GCGTC HincII 811 GTCAAC GT[CT]^[GA]AC 1627 GTTGAC HindII 811 GTCAAC GT[CT]^[GA]AC 1627 GTTGAC HindIII 1511 AAGCTT A^AGCTT 1931 AAGCTT MvnI 879 CGCG CG^CG 1608 CGCG NcoI 1212 CCATGG C^CATGG 1491 CCATGG PshBI 2962 ATTAAT AT^TAAT 4716 ATTAAT SspI 2783 AATATT AAT^ATT 4538 AATATT TaqI 2577 TCGA T^CGA 2910 TCGA TthHB8I 2577 TCGA T^CGA 2910 TCGA XmnI 2447 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4341 GAAACTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 97 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII BanII BbvI BciVI BcnI BfaI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI EarI EcoNI EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XhoII