##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII AccII AccIII AcyI AgeI
Aor51HI ApaI AscI AspI AviII
AvrII BamHI BbeI BbvCI BcgI
BciVI BclI BcnI BglI BlnI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BsrBI BsrFI
BssHII BssSI BstUI BstXI CfoI
Cfr10I ClaI CpoI DrdI EagI
EclXI Eco47III Eco52I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HpaII KasI
KspI MfeI MluI MroI MspI
MunI MvnI NaeI NarI NciI
NgoMIV NheI NotI NruI PacI
PflFI PinAI PmeI PshAI PvuI
RsrII SacI SacII SalI SapI
SbfI SfiI SfoI SgrAI SmaI
SnaBI Sse8387I Tth111I XcmI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AciI 1690 GCGG CCGC(-3/-1)
AclI 2671 AACGTT AA^CGTT
AflII 5090 CTTAAG C^TTAAG
AhdI 2287 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1069 GTGCAC G^TGCAC
AlwI 2961 GATCC GGATC(4/5)
ApaLI 1069 GTGCAC G^TGCAC
AspEI 2287 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 190 CTCGAG C^[CT]CG[GA]G
BbrPI 4234 CACGTG CAC^GTG
BfrI 5090 CTTAAG C^TTAAG
BsaBI 1040 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 2442 GAGGAG GAGGAG(10/8)
BsgI 2016 GTGCAG GTGCAG(16/14)
BsoBI 190 CTCGAG C^[CT]CG[GA]G
BspLU11I 5204 ACATGT A^CATGT
BstBI 1697 TTCGAA TT^CGAA
BstEII 4585 GGTGACC G^GT[ACGT]ACC
BstPI 4585 GGTGACC G^GT[ACGT]ACC
DraIII 2000 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2287 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 4585 GGTGACC G^GT[ACGT]ACC
EcoRI 4034 GAATTC G^AATTC
HpaI 2547 GTTAAC GTT^AAC
MamI 1040 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 3974 CCATGG C^CATGG
NdeI 3615 CATATG CA^TATG
NspV 1697 TTCGAA TT^CGAA
PaeR7I 190 CTCGAG C^TCGAG
PflMI 4590 CCATACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 4234 CACGTG CAC^GTG
PmlI 4234 CACGTG CAC^GTG
Psp1406I 2671 AACGTT AA^CGTT
SexAI 3034 ACCTGGT A^CC[AT]GGT
SfuI 1697 TTCGAA TT^CGAA
StuI 4310 AGGCCT AGG^CCT
SwaI 2737 ATTTAAAT ATTT^AAAT
Van91I 4590 CCATACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 5706 TCTAGA T^CTAGA
XhoI 190 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Acc65I 3177 GGTACC G^GTACC
5191 GGTACC
AocI 367 CCTCAGG CC^T[ACGT]AGG
1716 CCTCAGG
Asp718I 3177 GGTACC G^GTACC
5191 GGTACC
AspHI 1069 GTGCAC G[AT]GC[AT]^C
2503 GTGCTC
BanII 2194 GAGCCC G[GA]GC[CT]^C
3588 GAGCCC
BlpI 2569 GCTAAGC GC^T[ACGT]AGC
3809 GCTTAGC
Bpu1102I 2569 GCTAAGC GC^T[ACGT]AGC
3809 GCTTAGC
BsaAI 2945 TACGTG [CT]AC^GT[GA]
4234 CACGTG
BsaI 2340 GAGACC GGTCTC(1/5)
3748 GAGACC
BsiHKAI 1069 GTGCAC G[AT]GC[AT]^C
2503 GTGCTC
BsrDI 494 CATTGC GCAATG(2/0)
2800 GCAATG
Bsu36I 367 CCTCAGG CC^T[ACGT]AGG
1716 CCTCAGG
CelII 2569 GCTAAGC GC^T[ACGT]AGC
3809 GCTTAGC
Eco81I 367 CCTCAGG CC^T[ACGT]AGG
1716 CCTCAGG
EcoNI 1848 CCTTGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3914 CCTCTGCTAGG
EcoRV 1199 GATATC GAT^ATC
5059 GATATC
KpnI 3177 GGTACC GGTAC^C
5191 GGTACC
PpuMI 2285 AGGACCC [GA]G^G[AT]CC[CT]
5511 GGGACCT
SpeI 3725 ACTAGT A^CTAGT
5130 ACTAGT
SphI 465 GCATGC GCATG^C
5518 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccI AcsI AfaI AflIII AluI
AlwNI ApoI AseI AsnI Asp700I
AvaII BalI BanI BbsI BbvI
BfaI BglII BmyI BpmI BpuAI
BsaJI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I Bsp1407I BspHI BspMI
BsrGI BsrI BssKI Bst1107I BstAPI
BstF5I BstNI BstYI BstZ17I Cac8I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I EcoT22I Fnu4HI FokI HaeIII
HincII HindII HindIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NsiI
NspI PleI PshBI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI