################################## # NO CUT ENZYMES ( 91 enzymes) # ################################## AatII AccII AccIII AcyI AgeI Aor51HI ApaI AscI AspI AviII AvrII BamHI BbeI BbvCI BcgI BciVI BclI BcnI BglI BlnI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BsrBI BsrFI BssHII BssSI BstUI BstXI CfoI Cfr10I ClaI CpoI DrdI EagI EclXI Eco47III Eco52I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HpaII KasI KspI MfeI MluI MroI MspI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI PacI PflFI PinAI PmeI PshAI PvuI RsrII SacI SacII SalI SapI SbfI SfiI SfoI SgrAI SmaI SnaBI Sse8387I Tth111I XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AciI 1690 GCGG CCGC(-3/-1) AclI 2671 AACGTT AA^CGTT AflII 5090 CTTAAG C^TTAAG AhdI 2287 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1069 GTGCAC G^TGCAC AlwI 2961 GATCC GGATC(4/5) ApaLI 1069 GTGCAC G^TGCAC AspEI 2287 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 190 CTCGAG C^[CT]CG[GA]G BbrPI 4234 CACGTG CAC^GTG BfrI 5090 CTTAAG C^TTAAG BsaBI 1040 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 2442 GAGGAG GAGGAG(10/8) BsgI 2016 GTGCAG GTGCAG(16/14) BsoBI 190 CTCGAG C^[CT]CG[GA]G BspLU11I 5204 ACATGT A^CATGT BstBI 1697 TTCGAA TT^CGAA BstEII 4585 GGTGACC G^GT[ACGT]ACC BstPI 4585 GGTGACC G^GT[ACGT]ACC DraIII 2000 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 2287 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 4585 GGTGACC G^GT[ACGT]ACC EcoRI 4034 GAATTC G^AATTC HpaI 2547 GTTAAC GTT^AAC MamI 1040 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NcoI 3974 CCATGG C^CATGG NdeI 3615 CATATG CA^TATG NspV 1697 TTCGAA TT^CGAA PaeR7I 190 CTCGAG C^TCGAG PflMI 4590 CCATACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 4234 CACGTG CAC^GTG PmlI 4234 CACGTG CAC^GTG Psp1406I 2671 AACGTT AA^CGTT SexAI 3034 ACCTGGT A^CC[AT]GGT SfuI 1697 TTCGAA TT^CGAA StuI 4310 AGGCCT AGG^CCT SwaI 2737 ATTTAAAT ATTT^AAAT Van91I 4590 CCATACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 5706 TCTAGA T^CTAGA XhoI 190 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### Acc65I 3177 GGTACC G^GTACC 5191 GGTACC AocI 367 CCTCAGG CC^T[ACGT]AGG 1716 CCTCAGG Asp718I 3177 GGTACC G^GTACC 5191 GGTACC AspHI 1069 GTGCAC G[AT]GC[AT]^C 2503 GTGCTC BanII 2194 GAGCCC G[GA]GC[CT]^C 3588 GAGCCC BlpI 2569 GCTAAGC GC^T[ACGT]AGC 3809 GCTTAGC Bpu1102I 2569 GCTAAGC GC^T[ACGT]AGC 3809 GCTTAGC BsaAI 2945 TACGTG [CT]AC^GT[GA] 4234 CACGTG BsaI 2340 GAGACC GGTCTC(1/5) 3748 GAGACC BsiHKAI 1069 GTGCAC G[AT]GC[AT]^C 2503 GTGCTC BsrDI 494 CATTGC GCAATG(2/0) 2800 GCAATG Bsu36I 367 CCTCAGG CC^T[ACGT]AGG 1716 CCTCAGG CelII 2569 GCTAAGC GC^T[ACGT]AGC 3809 GCTTAGC Eco81I 367 CCTCAGG CC^T[ACGT]AGG 1716 CCTCAGG EcoNI 1848 CCTTGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3914 CCTCTGCTAGG EcoRV 1199 GATATC GAT^ATC 5059 GATATC KpnI 3177 GGTACC GGTAC^C 5191 GGTACC PpuMI 2285 AGGACCC [GA]G^G[AT]CC[CT] 5511 GGGACCT SpeI 3725 ACTAGT A^CTAGT 5130 ACTAGT SphI 465 GCATGC GCATG^C 5518 GCATGC ######################################### # 3 OR MORE CUT ENZYMES ( 107 enzymes) # ######################################### AccI AcsI AfaI AflIII AluI AlwNI ApoI AseI AsnI Asp700I AvaII BalI BanI BbsI BbvI BfaI BglII BmyI BpmI BpuAI BsaJI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspHI BspMI BsrGI BsrI BssKI Bst1107I BstAPI BstF5I BstNI BstYI BstZ17I Cac8I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HincII HindII HindIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV NsiI NspI PleI PshBI PstI PvuII RcaI RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI