################################## # NO CUT ENZYMES ( 103 enzymes) # ################################## AatII AccI AccIII AclI AcyI AflIII AhdI AocI ApaI AscI Asp700I AspEI AspI AvrII BbeI BbrPI BbsI BcgI BciVI BclI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoRV FauI FbaI FseI HgaI Hin1I HincII HindII HpaI KasI KspI MaeII MfeI MluI MroI MunI NarI NdeI NotI NruI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfoI SfuI SgrAI SnaBI Sse8387I SspBI SspI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### Acc65I 4273 GGTACC G^GTACC AccII 1360 CGCG CG^CG AgeI 5149 ACCGGT A^CCGGT AseI 2431 ATTAAT AT^TAAT AsnI 2431 ATTAAT AT^TAAT Asp718I 4273 GGTACC G^GTACC BalI 764 TGGCCA TGG^CCA BsaWI 5149 ACCGGT [AT]^CCGG[AT] BseRI 1045 CTCCTC GAGGAG(10/8) BspHI 1073 TCATGA T^CATGA BspMI 414 ACCTGC ACCTGC(4/8) BstEII 4564 GGTAACC G^GT[ACGT]ACC BstPI 4564 GGTAACC G^GT[ACGT]ACC BstUI 1360 CGCG CG^CG EaeI 764 TGGCCA [CT]^GGCC[GA] EcoO65I 4564 GGTAACC G^GT[ACGT]ACC EcoRI 1252 GAATTC G^AATTC EcoT22I 215 ATGCAT ATGCA^T HindIII 4296 AAGCTT A^AGCTT KpnI 4273 GGTACC GGTAC^C MluNI 764 TGGCCA TGG^CCA MscI 764 TGGCCA TGG^CCA MvnI 1360 CGCG CG^CG NaeI 3310 GCCGGC GCC^GGC NgoMIV 3310 GCCGGC G^CCGGC NsiI 215 ATGCAT ATGCA^T NspI 1312 GCATGC [GA]CATG^[CT] PinAI 5149 ACCGGT A^CCGGT PshBI 2431 ATTAAT AT^TAAT RcaI 1073 TCATGA T^CATGA SacI 542 GAGCTC GAGCT^C SapI 2909 GCTCTTC GCTCTTC(1/4) ScaI 2170 AGTACT AGT^ACT SfiI 549 GGCCTCTGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 656 CCCGGG CCC^GGG SphI 1312 GCATGC GCATG^C XmaCI 656 CCCGGG C^CCGGG XmaI 656 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AflII 2289 CTTAAG C^TTAAG 2391 CTTAAG Aor51HI 839 AGCGCT AGC^GCT 4489 AGCGCT AvaI 656 CCCGGG C^[CT]CG[GA]G 755 CCCGAG AviII 1118 TGCGCA TGC^GCA 1530 TGCGCA BamHI 725 GGATCC G^GATCC 2449 GGATCC BbvCI 1405 GCTGAGG CCTCAGC(-5/-2) 1423 GCTGAGG BcnI 656 CCCGG CC^[GC]GG 657 CCGGG BfrI 2289 CTTAAG C^TTAAG 2391 CTTAAG BsaBI 613 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 828 GATCCCAATC BsaI 1042 GGTCTC GGTCTC(1/5) 4040 GGTCTC BsoBI 656 CCCGGG C^[CT]CG[GA]G 755 CCCGAG BsrFI 3310 GCCGGC [GA]^CCGG[CT] 5149 ACCGGT Cfr10I 3310 GCCGGC [GA]^CCGG[CT] 5149 ACCGGT Eco47III 839 AGCGCT AGC^GCT 4489 AGCGCT FspI 1118 TGCGCA TGC^GCA 1530 TGCGCA MamI 613 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 828 GATCCCAATC MslI 685 CATCCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1068 CATTTTCATG NciI 656 CCCGG CC^[GC]GG 657 CCGGG NheI 3610 GCTAGC G^CTAGC 4312 GCTAGC SmlI 2289 CTTAAG C^T[CT][GA]AG 2391 CTTAAG TaqI 483 TCGA T^CGA 933 TCGA TthHB8I 483 TCGA T^CGA 933 TCGA XcmI 1437 CCAAATGGGGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 5181 CCATGGTGGCTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 94 enzymes) # ######################################### AciI AcsI AfaI AluI Alw44I AlwI AlwNI ApaLI ApoI AspHI AvaII BanI BanII BbvI BfaI BglI BmyI BpmI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrDI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NcoI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SpeI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XhoII