##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflIII AhdI AocI ApaI AscI
Asp700I AspEI AspI AvrII BbeI
BbrPI BbsI BcgI BciVI BclI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaHI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstBI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoRV FauI FbaI FseI
HgaI Hin1I HincII HindII HpaI
KasI KspI MaeII MfeI MluI
MroI MunI NarI NdeI NotI
NruI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfoI SfuI SgrAI SnaBI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 4273 GGTACC G^GTACC
AccII 1360 CGCG CG^CG
AgeI 5149 ACCGGT A^CCGGT
AseI 2431 ATTAAT AT^TAAT
AsnI 2431 ATTAAT AT^TAAT
Asp718I 4273 GGTACC G^GTACC
BalI 764 TGGCCA TGG^CCA
BsaWI 5149 ACCGGT [AT]^CCGG[AT]
BseRI 1045 CTCCTC GAGGAG(10/8)
BspHI 1073 TCATGA T^CATGA
BspMI 414 ACCTGC ACCTGC(4/8)
BstEII 4564 GGTAACC G^GT[ACGT]ACC
BstPI 4564 GGTAACC G^GT[ACGT]ACC
BstUI 1360 CGCG CG^CG
EaeI 764 TGGCCA [CT]^GGCC[GA]
EcoO65I 4564 GGTAACC G^GT[ACGT]ACC
EcoRI 1252 GAATTC G^AATTC
EcoT22I 215 ATGCAT ATGCA^T
HindIII 4296 AAGCTT A^AGCTT
KpnI 4273 GGTACC GGTAC^C
MluNI 764 TGGCCA TGG^CCA
MscI 764 TGGCCA TGG^CCA
MvnI 1360 CGCG CG^CG
NaeI 3310 GCCGGC GCC^GGC
NgoMIV 3310 GCCGGC G^CCGGC
NsiI 215 ATGCAT ATGCA^T
NspI 1312 GCATGC [GA]CATG^[CT]
PinAI 5149 ACCGGT A^CCGGT
PshBI 2431 ATTAAT AT^TAAT
RcaI 1073 TCATGA T^CATGA
SacI 542 GAGCTC GAGCT^C
SapI 2909 GCTCTTC GCTCTTC(1/4)
ScaI 2170 AGTACT AGT^ACT
SfiI 549 GGCCTCTGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 656 CCCGGG CCC^GGG
SphI 1312 GCATGC GCATG^C
XmaCI 656 CCCGGG C^CCGGG
XmaI 656 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflII 2289 CTTAAG C^TTAAG
2391 CTTAAG
Aor51HI 839 AGCGCT AGC^GCT
4489 AGCGCT
AvaI 656 CCCGGG C^[CT]CG[GA]G
755 CCCGAG
AviII 1118 TGCGCA TGC^GCA
1530 TGCGCA
BamHI 725 GGATCC G^GATCC
2449 GGATCC
BbvCI 1405 GCTGAGG CCTCAGC(-5/-2)
1423 GCTGAGG
BcnI 656 CCCGG CC^[GC]GG
657 CCGGG
BfrI 2289 CTTAAG C^TTAAG
2391 CTTAAG
BsaBI 613 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
828 GATCCCAATC
BsaI 1042 GGTCTC GGTCTC(1/5)
4040 GGTCTC
BsoBI 656 CCCGGG C^[CT]CG[GA]G
755 CCCGAG
BsrFI 3310 GCCGGC [GA]^CCGG[CT]
5149 ACCGGT
Cfr10I 3310 GCCGGC [GA]^CCGG[CT]
5149 ACCGGT
Eco47III 839 AGCGCT AGC^GCT
4489 AGCGCT
FspI 1118 TGCGCA TGC^GCA
1530 TGCGCA
MamI 613 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
828 GATCCCAATC
MslI 685 CATCCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1068 CATTTTCATG
NciI 656 CCCGG CC^[GC]GG
657 CCGGG
NheI 3610 GCTAGC G^CTAGC
4312 GCTAGC
SmlI 2289 CTTAAG C^T[CT][GA]AG
2391 CTTAAG
TaqI 483 TCGA T^CGA
933 TCGA
TthHB8I 483 TCGA T^CGA
933 TCGA
XcmI 1437 CCAAATGGGGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5181 CCATGGTGGCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI AlwNI ApaLI ApoI AspHI
AvaII BanI BanII BbvI BfaI
BglI BmyI BpmI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NcoI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SpeI StuI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XhoII