##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
Acc65I AccIII AhdI ApaI AscI
Asp718I AspEI AvrII BbeI BbvCI
BclI BglII BlnI BsaBI BseAI
BsiEI BsiWI BspDI BspEI BsrBI
BssHII ClaI CpoI DrdI EagI
Eam1105I EclXI Eco52I EcoNI EcoRV
FbaI FseI HpaI KasI KpnI
KspI MamI MluI MroI NaeI
NarI NgoMIV NotI NruI PacI
PaeR7I PmeI PshAI PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SnaBI Sse8387I StuI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AatII 748 GACGTC GACGT^C
AclI 2568 AACGTT AA^CGTT
AcyI 748 GACGTC G[GA]^CG[CT]C
AflII 2819 CTTAAG C^TTAAG
AgeI 2175 ACCGGT A^CCGGT
AocI 396 CCTCAGG CC^T[ACGT]AGG
Aor51HI 4372 AGCGCT AGC^GCT
AspI 128 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4474 TGCGCA TGC^GCA
BalI 5654 TGGCCA TGG^CCA
BamHI 2383 GGATCC G^GATCC
BanI 5370 GGCACC G^G[CT][GA]CC
BanII 1238 GAGCCC G[GA]GC[CT]^C
BbrPI 980 CACGTG CAC^GTG
BcgI 1879 CGAGAAGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2819 CTTAAG C^TTAAG
BglI 5362 GCCCTGTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 3938 CTCCAG CTGGAG(16/14)
BsaHI 748 GACGTC G[GA]^CG[CT]C
BsaWI 2175 ACCGGT [AT]^CCGG[AT]
BsmBI 4406 GAGACG CGTCTC(1/5)
BssSI 4110 CACGAG CACGAG(-5/-1)
BstBI 2233 TTCGAA TT^CGAA
BstXI 2160 CCAGCAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 2383 GGATCC [GA]^GATC[CT]
Bsu36I 396 CCTCAGG CC^T[ACGT]AGG
Eco47III 4372 AGCGCT AGC^GCT
Eco81I 396 CCTCAGG CC^T[ACGT]AGG
FauI 1472 CCCGC CCCGC(4/6)
FspI 4474 TGCGCA TGC^GCA
HaeII 4372 AGCGCT [GA]GCGC^[CT]
HgaI 2305 GCGTC GACGC(5/10)
Hin1I 748 GACGTC G[GA]^CG[CT]C
HindIII 2799 AAGCTT A^AGCTT
MfeI 3992 CAATTG C^AATTG
MflI 2383 GGATCC [GA]^GATC[CT]
MluNI 5654 TGGCCA TGG^CCA
MscI 5654 TGGCCA TGG^CCA
MunI 3992 CAATTG C^AATTG
NdeI 455 CATATG CA^TATG
NspV 2233 TTCGAA TT^CGAA
PflFI 128 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 629 CCAACTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2175 ACCGGT A^CCGGT
PmaCI 980 CACGTG CAC^GTG
PmlI 980 CACGTG CAC^GTG
PpuMI 2713 AGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 2568 AACGTT AA^CGTT
SfuI 2233 TTCGAA TT^CGAA
SmaI 1645 CCCGGG CCC^GGG
Tth111I 128 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 629 CCAACTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1129 TCTAGA T^CTAGA
XhoII 2383 GGATCC [GA]^GATC[CT]
XmaCI 1645 CCCGGG C^CCGGG
XmaI 1645 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccII 3055 CGCG CG^CG
5411 CGCG
AseI 3241 ATTAAT AT^TAAT
4944 ATTAAT
AsnI 3241 ATTAAT AT^TAAT
4944 ATTAAT
AvaI 1645 CCCGGG C^[CT]CG[GA]G
2196 CTCGGG
BbsI 125 GAAGAC GAAGAC(2/6)
3848 GAAGAC
BpuAI 125 GAAGAC GAAGAC(2/6)
3848 GAAGAC
BsaAI 980 CACGTG [CT]AC^GT[GA]
4130 CACGTA
BseRI 5714 CTCCTC GAGGAG(10/8)
3262 GAGGAG
BsoBI 1645 CCCGGG C^[CT]CG[GA]G
2196 CTCGGG
Bsp1407I 2412 TGTACA T^GTACA
5458 TGTACA
BspHI 1729 TCATGA T^CATGA
3614 TCATGA
BspLU11I 879 ACATGT A^CATGT
2366 ACATGT
BsrFI 431 GCCGGT [GA]^CCGG[CT]
2175 ACCGGT
BsrGI 2412 TGTACA T^GTACA
5458 TGTACA
Bst1107I 4646 GTATAC GTA^TAC
5008 GTATAC
BstAPI 2645 GCAGAGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2987 GCAGTGCTTGC
BstEII 3412 GGTGACC G^GT[ACGT]ACC
3681 GGTGACC
BstPI 3412 GGTGACC G^GT[ACGT]ACC
3681 GGTGACC
BstUI 3055 CGCG CG^CG
5411 CGCG
BstZ17I 4646 GTATAC GTA^TAC
5008 GTATAC
Cfr10I 431 GCCGGT [GA]^CCGG[CT]
2175 ACCGGT
DraII 2713 AGGACCC [GA]G^G[ACGT]CC[CT]
4011 GGGGCCT
DraIII 4378 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
5020 CACTGTGTG
DsaI 2599 CCATGG C^C[GA][CT]GG
2830 CCATGG
EcoO109I 2713 AGGACCC [GA]G^G[ACGT]CC[CT]
4011 GGGGCCT
EcoO65I 3412 GGTGACC G^GT[ACGT]ACC
3681 GGTGACC
EcoRI 1745 GAATTC G^AATTC
2825 GAATTC
MvnI 3055 CGCG CG^CG
5411 CGCG
NcoI 2599 CCATGG C^CATGG
2830 CCATGG
NheI 866 GCTAGC G^CTAGC
2435 GCTAGC
PshBI 3241 ATTAAT AT^TAAT
4944 ATTAAT
RcaI 1729 TCATGA T^CATGA
3614 TCATGA
SpeI 1403 ACTAGT A^CTAGT
3088 ACTAGT
SphI 2037 GCATGC GCATG^C
5080 GCATGC
SspBI 2412 TGTACA T^GTACA
5458 TGTACA
SspI 3158 AATATT AAT^ATT
5623 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaLI
ApoI Asp700I AspHI AvaII BbvI
BciVI BcnI BfaI BlpI BmyI
Bpu1102I BsaI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI EaeI
EarI EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NsiI NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XmnI