################################## # NO CUT ENZYMES ( 62 enzymes) # ################################## AclI AflII Aor51HI AscI AseI AsnI AviII BbeI BfrI BsaBI BsiEI BsiWI BssHII Bst1107I BstBI BstEII BstPI BstZ17I CpoI EagI EclXI Eco47III Eco52I EcoO65I EcoRI FseI FspI HindIII HpaI KasI KspI MamI MfeI MluI MunI NaeI NarI NgoMIV NotI NruI NspV PacI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AatII 1841 GACGTC GACGT^C AccI 3546 GTCTAC GT^[AC][GT]AC AgeI 464 ACCGGT A^CCGGT AhdI 4219 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2057 GTGCAC G^TGCAC ApaLI 2057 GTGCAC G^TGCAC Asp700I 440 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 4219 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 792 GGATCC G^GATCC BbrPI 2042 CACGTG CAC^GTG BclI 688 TGATCA T^GATCA BglII 2784 AGATCT A^GATCT BlpI 3056 GCTGAGC GC^T[ACGT]AGC Bpu1102I 3056 GCTGAGC GC^T[ACGT]AGC BsmBI 2396 GAGACG CGTCTC(1/5) BspDI 2033 ATCGAT AT^CGAT BspLU11I 4281 ACATGT A^CATGT BstXI 805 CCACTCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 3056 GCTGAGC GC^T[ACGT]AGC ClaI 2033 ATCGAT AT^CGAT DraI 2873 TTTAAA TTT^AAA Eam1105I 4219 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRV 371 GATATC GAT^ATC FbaI 688 TGATCA T^GATCA HincII 3516 GTCAAC GT[CT]^[GA]AC HindII 3516 GTCAAC GT[CT]^[GA]AC NheI 1630 GCTAGC G^CTAGC PaeR7I 2564 CTCGAG C^TCGAG PinAI 464 ACCGGT A^CCGGT PmaCI 2042 CACGTG CAC^GTG PmlI 2042 CACGTG CAC^GTG SbfI 3339 CCTGCAGG CCTGCA^GG SphI 4233 GCATGC GCATG^C Sse8387I 3339 CCTGCAGG CCTGCA^GG XhoI 2564 CTCGAG C^TCGAG XmnI 440 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AccIII 795 TCCGGA T^CCGGA 1374 TCCGGA AcsI 212 AAATTC [GA]^AATT[CT] 3038 AAATTC AcyI 288 GACGCC G[GA]^CG[CT]C 1841 GACGTC ApoI 212 AAATTC [GA]^AATT[CT] 3038 AAATTC AspI 137 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC 3098 GACTTGGTC AvrII 3660 CCTAGG C^CTAGG 3875 CCTAGG BalI 1861 TGGCCA TGG^CCA 3770 TGGCCA BciVI 1906 GGATAC GTATCC(6/5) 738 GTATCC BglI 1149 GCCAGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1567 GCCCCTCCGGC BlnI 3660 CCTAGG C^CTAGG 3875 CCTAGG BsaHI 288 GACGCC G[GA]^CG[CT]C 1841 GACGTC BseAI 795 TCCGGA T^CCGGA 1374 TCCGGA Bsp1407I 553 TGTACA T^GTACA 3827 TGTACA BspEI 795 TCCGGA T^CCGGA 1374 TCCGGA BspHI 1014 TCATGA T^CATGA 2706 TCATGA BsrFI 464 ACCGGT [GA]^CCGG[CT] 831 ACCGGC BsrGI 553 TGTACA T^GTACA 3827 TGTACA Cfr10I 464 ACCGGT [GA]^CCGG[CT] 831 ACCGGC DrdI 1663 GACACACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4492 GACTCCTCAGTC EcoT22I 2813 ATGCAT ATGCA^T 4663 ATGCAT Hin1I 288 GACGCC G[GA]^CG[CT]C 1841 GACGTC MluNI 1861 TGGCCA TGG^CCA 3770 TGGCCA MroI 795 TCCGGA T^CCGGA 1374 TCCGGA MscI 1861 TGGCCA TGG^CCA 3770 TGGCCA NcoI 120 CCATGG C^CATGG 2745 CCATGG NdeI 508 CATATG CA^TATG 1524 CATATG NsiI 2813 ATGCAT ATGCA^T 4663 ATGCAT PflFI 137 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC 3098 GACTTGGTC PflMI 2165 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2782 CCAGATCTTGG RcaI 1014 TCATGA T^CATGA 2706 TCATGA SacI 2435 GAGCTC GAGCT^C 3189 GAGCTC SspBI 553 TGTACA T^GTACA 3827 TGTACA Tth111I 137 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC 3098 GACTTGGTC Van91I 2165 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2782 CCAGATCTTGG XcmI 1864 CCACTACAAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4310 CCACACCTGGCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 125 enzymes) # ######################################### Acc65I AccII AciI AfaI AflIII AluI AlwI AlwNI AocI ApaI Asp718I AspHI AvaI AvaII BanI BanII BbsI BbvCI BbvI BcgI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmaCI XmaI