##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AclI AflII Aor51HI AscI AseI
AsnI AviII BbeI BfrI BsaBI
BsiEI BsiWI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I CpoI EagI
EclXI Eco47III Eco52I EcoO65I EcoRI
FseI FspI HindIII HpaI KasI
KspI MamI MfeI MluI MunI
NaeI NarI NgoMIV NotI NruI
NspV PacI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SspI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 1841 GACGTC GACGT^C
AccI 3546 GTCTAC GT^[AC][GT]AC
AgeI 464 ACCGGT A^CCGGT
AhdI 4219 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2057 GTGCAC G^TGCAC
ApaLI 2057 GTGCAC G^TGCAC
Asp700I 440 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4219 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 792 GGATCC G^GATCC
BbrPI 2042 CACGTG CAC^GTG
BclI 688 TGATCA T^GATCA
BglII 2784 AGATCT A^GATCT
BlpI 3056 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3056 GCTGAGC GC^T[ACGT]AGC
BsmBI 2396 GAGACG CGTCTC(1/5)
BspDI 2033 ATCGAT AT^CGAT
BspLU11I 4281 ACATGT A^CATGT
BstXI 805 CCACTCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 3056 GCTGAGC GC^T[ACGT]AGC
ClaI 2033 ATCGAT AT^CGAT
DraI 2873 TTTAAA TTT^AAA
Eam1105I 4219 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 371 GATATC GAT^ATC
FbaI 688 TGATCA T^GATCA
HincII 3516 GTCAAC GT[CT]^[GA]AC
HindII 3516 GTCAAC GT[CT]^[GA]AC
NheI 1630 GCTAGC G^CTAGC
PaeR7I 2564 CTCGAG C^TCGAG
PinAI 464 ACCGGT A^CCGGT
PmaCI 2042 CACGTG CAC^GTG
PmlI 2042 CACGTG CAC^GTG
SbfI 3339 CCTGCAGG CCTGCA^GG
SphI 4233 GCATGC GCATG^C
Sse8387I 3339 CCTGCAGG CCTGCA^GG
XhoI 2564 CTCGAG C^TCGAG
XmnI 440 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccIII 795 TCCGGA T^CCGGA
1374 TCCGGA
AcsI 212 AAATTC [GA]^AATT[CT]
3038 AAATTC
AcyI 288 GACGCC G[GA]^CG[CT]C
1841 GACGTC
ApoI 212 AAATTC [GA]^AATT[CT]
3038 AAATTC
AspI 137 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3098 GACTTGGTC
AvrII 3660 CCTAGG C^CTAGG
3875 CCTAGG
BalI 1861 TGGCCA TGG^CCA
3770 TGGCCA
BciVI 1906 GGATAC GTATCC(6/5)
738 GTATCC
BglI 1149 GCCAGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1567 GCCCCTCCGGC
BlnI 3660 CCTAGG C^CTAGG
3875 CCTAGG
BsaHI 288 GACGCC G[GA]^CG[CT]C
1841 GACGTC
BseAI 795 TCCGGA T^CCGGA
1374 TCCGGA
Bsp1407I 553 TGTACA T^GTACA
3827 TGTACA
BspEI 795 TCCGGA T^CCGGA
1374 TCCGGA
BspHI 1014 TCATGA T^CATGA
2706 TCATGA
BsrFI 464 ACCGGT [GA]^CCGG[CT]
831 ACCGGC
BsrGI 553 TGTACA T^GTACA
3827 TGTACA
Cfr10I 464 ACCGGT [GA]^CCGG[CT]
831 ACCGGC
DrdI 1663 GACACACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4492 GACTCCTCAGTC
EcoT22I 2813 ATGCAT ATGCA^T
4663 ATGCAT
Hin1I 288 GACGCC G[GA]^CG[CT]C
1841 GACGTC
MluNI 1861 TGGCCA TGG^CCA
3770 TGGCCA
MroI 795 TCCGGA T^CCGGA
1374 TCCGGA
MscI 1861 TGGCCA TGG^CCA
3770 TGGCCA
NcoI 120 CCATGG C^CATGG
2745 CCATGG
NdeI 508 CATATG CA^TATG
1524 CATATG
NsiI 2813 ATGCAT ATGCA^T
4663 ATGCAT
PflFI 137 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3098 GACTTGGTC
PflMI 2165 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2782 CCAGATCTTGG
RcaI 1014 TCATGA T^CATGA
2706 TCATGA
SacI 2435 GAGCTC GAGCT^C
3189 GAGCTC
SspBI 553 TGTACA T^GTACA
3827 TGTACA
Tth111I 137 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3098 GACTTGGTC
Van91I 2165 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2782 CCAGATCTTGG
XcmI 1864 CCACTACAAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4310 CCACACCTGGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
Acc65I AccII AciI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
Asp718I AspHI AvaI AvaII BanI
BanII BbsI BbvCI BbvI BcgI
BcnI BfaI BmyI BpmI BpuAI
BsaAI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI KpnI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmaCI XmaI