##################################
# NO CUT ENZYMES (   62 enzymes) #
##################################

AclI        AflII       Aor51HI     AscI        AseI        
AsnI        AviII       BbeI        BfrI        BsaBI       
BsiEI       BsiWI       BssHII      Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     CpoI        EagI        
EclXI       Eco47III    Eco52I      EcoO65I     EcoRI       
FseI        FspI        HindIII     HpaI        KasI        
KspI        MamI        MfeI        MluI        MunI        
NaeI        NarI        NgoMIV      NotI        NruI        
NspV        PacI        PmeI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacII       SalI        
SapI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        SspI        
SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AatII        1841  GACGTC               GACGT^C
AccI         3546  GTCTAC               GT^[AC][GT]AC
AgeI          464  ACCGGT               A^CCGGT
AhdI         4219  GACCTGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       2057  GTGCAC               G^TGCAC
ApaLI        2057  GTGCAC               G^TGCAC
Asp700I       440  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        4219  GACCTGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI         792  GGATCC               G^GATCC
BbrPI        2042  CACGTG               CAC^GTG
BclI          688  TGATCA               T^GATCA
BglII        2784  AGATCT               A^GATCT
BlpI         3056  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     3056  GCTGAGC              GC^T[ACGT]AGC
BsmBI        2396  GAGACG               CGTCTC(1/5)
BspDI        2033  ATCGAT               AT^CGAT
BspLU11I     4281  ACATGT               A^CATGT
BstXI         805  CCACTCTAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII        3056  GCTGAGC              GC^T[ACGT]AGC
ClaI         2033  ATCGAT               AT^CGAT
DraI         2873  TTTAAA               TTT^AAA
Eam1105I     4219  GACCTGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV         371  GATATC               GAT^ATC
FbaI          688  TGATCA               T^GATCA
HincII       3516  GTCAAC               GT[CT]^[GA]AC
HindII       3516  GTCAAC               GT[CT]^[GA]AC
NheI         1630  GCTAGC               G^CTAGC
PaeR7I       2564  CTCGAG               C^TCGAG
PinAI         464  ACCGGT               A^CCGGT
PmaCI        2042  CACGTG               CAC^GTG
PmlI         2042  CACGTG               CAC^GTG
SbfI         3339  CCTGCAGG             CCTGCA^GG
SphI         4233  GCATGC               GCATG^C
Sse8387I     3339  CCTGCAGG             CCTGCA^GG
XhoI         2564  CTCGAG               C^TCGAG
XmnI          440  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AccIII        795  TCCGGA               T^CCGGA
             1374  TCCGGA              
AcsI          212  AAATTC               [GA]^AATT[CT]
             3038  AAATTC              
AcyI          288  GACGCC               G[GA]^CG[CT]C
             1841  GACGTC              
ApoI          212  AAATTC               [GA]^AATT[CT]
             3038  AAATTC              
AspI          137  GACGCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3098  GACTTGGTC           
AvrII        3660  CCTAGG               C^CTAGG
             3875  CCTAGG              
BalI         1861  TGGCCA               TGG^CCA
             3770  TGGCCA              
BciVI        1906  GGATAC               GTATCC(6/5)
              738  GTATCC              
BglI         1149  GCCAGGATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1567  GCCCCTCCGGC         
BlnI         3660  CCTAGG               C^CTAGG
             3875  CCTAGG              
BsaHI         288  GACGCC               G[GA]^CG[CT]C
             1841  GACGTC              
BseAI         795  TCCGGA               T^CCGGA
             1374  TCCGGA              
Bsp1407I      553  TGTACA               T^GTACA
             3827  TGTACA              
BspEI         795  TCCGGA               T^CCGGA
             1374  TCCGGA              
BspHI        1014  TCATGA               T^CATGA
             2706  TCATGA              
BsrFI         464  ACCGGT               [GA]^CCGG[CT]
              831  ACCGGC              
BsrGI         553  TGTACA               T^GTACA
             3827  TGTACA              
Cfr10I        464  ACCGGT               [GA]^CCGG[CT]
              831  ACCGGC              
DrdI         1663  GACACACCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4492  GACTCCTCAGTC        
EcoT22I      2813  ATGCAT               ATGCA^T
             4663  ATGCAT              
Hin1I         288  GACGCC               G[GA]^CG[CT]C
             1841  GACGTC              
MluNI        1861  TGGCCA               TGG^CCA
             3770  TGGCCA              
MroI          795  TCCGGA               T^CCGGA
             1374  TCCGGA              
MscI         1861  TGGCCA               TGG^CCA
             3770  TGGCCA              
NcoI          120  CCATGG               C^CATGG
             2745  CCATGG              
NdeI          508  CATATG               CA^TATG
             1524  CATATG              
NsiI         2813  ATGCAT               ATGCA^T
             4663  ATGCAT              
PflFI         137  GACGCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3098  GACTTGGTC           
PflMI        2165  CCAGGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2782  CCAGATCTTGG         
RcaI         1014  TCATGA               T^CATGA
             2706  TCATGA              
SacI         2435  GAGCTC               GAGCT^C
             3189  GAGCTC              
SspBI         553  TGTACA               T^GTACA
             3827  TGTACA              
Tth111I       137  GACGCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3098  GACTTGGTC           
Van91I       2165  CCAGGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2782  CCAGATCTTGG         
XcmI         1864  CCACTACAAGTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             4310  CCACACCTGGCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  125 enzymes) #
#########################################

Acc65I      AccII       AciI        AfaI        AflIII      
AluI        AlwI        AlwNI       AocI        ApaI        
Asp718I     AspHI       AvaI        AvaII       BanI        
BanII       BbsI        BbvCI       BbvI        BcgI        
BcnI        BfaI        BmyI        BpmI        BpuAI       
BsaAI       BsaI        BsaJI       BsaWI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsoBI       Bsp1286I    BspMI       
BsrBI       BsrDI       BsrI        BssKI       BssSI       
BstAPI      BstF5I      BstNI       BstUI       BstYI       
Bsu36I      Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DraIII      DsaI        EaeI        
EarI        Eco81I      EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        KpnI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PleI        PpuMI       PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmaI        SmlI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       XmaCI       XmaI