##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AclI AcsI AflII AgeI AhdI
Aor51HI ApoI AscI AseI AsnI
AspEI AvrII BamHI BbeI BbrPI
BciVI BfrI BglI BglII BlnI
BsaAI BsaBI BsiEI BsiWI BspDI
BspLU11I BsrFI BssHII BssSI BstBI
BstEII BstPI Cfr10I ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV FseI HpaI
KasI KspI MamI MfeI MluI
MunI NaeI NarI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacII SalI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SspI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 458 GGTACC G^GTACC
AccII 808 CGCG CG^CG
AccIII 766 TCCGGA T^CCGGA
AflIII 3251 ACACGT A^C[GA][CT]GT
Asp700I 3618 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 458 GGTACC G^GTACC
BclI 4107 TGATCA T^GATCA
BsaWI 766 TCCGGA [AT]^CCGG[AT]
BseAI 766 TCCGGA T^CCGGA
BsmI 3917 GAATGC GAATGC(1/-1)
BspEI 766 TCCGGA T^CCGGA
BspHI 4334 TCATGA T^CATGA
BsrBI 2268 GAGCGG CCGCTC(-3/-3)
Bst1107I 4149 GTATAC GTA^TAC
BstUI 808 CGCG CG^CG
BstZ17I 4149 GTATAC GTA^TAC
DraIII 398 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT22I 3885 ATGCAT ATGCA^T
FbaI 4107 TGATCA T^GATCA
HaeII 2158 AGCGCC [GA]GCGC^[CT]
HincII 2204 GTTGAC GT[CT]^[GA]AC
HindII 2204 GTTGAC GT[CT]^[GA]AC
HindIII 3159 AAGCTT A^AGCTT
KpnI 458 GGTACC GGTAC^C
MroI 766 TCCGGA T^CCGGA
MvnI 808 CGCG CG^CG
NdeI 4154 CATATG CA^TATG
NsiI 3885 ATGCAT ATGCA^T
RcaI 4334 TCATGA T^CATGA
SacI 2604 GAGCTC GAGCT^C
SphI 3883 GCATGC GCATG^C
XmnI 3618 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AatII 435 GACGTC GACGT^C
1608 GACGTC
AccI 3584 GTCTAC GT^[AC][GT]AC
4149 GTATAC
ApaI 2837 GGGCCC GGGCC^C
3045 GGGCCC
AspI 1206 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1839 GACAAGGTC
BbsI 792 GAAGAC GAAGAC(2/6)
733 GTCTTC
BcgI 1553 CGAGGACCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2062 GCATTGAGATCG
BlpI 2258 GCTGAGC GC^T[ACGT]AGC
2999 GCTCAGC
Bpu1102I 2258 GCTGAGC GC^T[ACGT]AGC
2999 GCTCAGC
BpuAI 792 GAAGAC GAAGAC(2/6)
733 GTCTTC
BsmBI 437 CGTCTC CGTCTC(1/5)
1859 GAGACG
Bsp1407I 1147 TGTACA T^GTACA
4010 TGTACA
BsrGI 1147 TGTACA T^GTACA
4010 TGTACA
CelII 2258 GCTGAGC GC^T[ACGT]AGC
2999 GCTCAGC
NcoI 1390 CCATGG C^CATGG
1406 CCATGG
PflFI 1206 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1839 GACAAGGTC
PflMI 238 CCAAAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4374 CCAGGGCATGG
PshAI 1861 GACGGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3598 GACAATAGTC
SmaI 864 CCCGGG CCC^GGG
912 CCCGGG
SspBI 1147 TGTACA T^GTACA
4010 TGTACA
Tth111I 1206 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1839 GACAAGGTC
Van91I 238 CCAAAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4374 CCAGGGCATGG
XmaCI 864 CCCGGG C^CCGGG
912 CCCGGG
XmaI 864 CCCGGG C^CCGGG
912 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AciI AcyI AfaI AluI Alw44I
AlwI AlwNI AocI ApaLI AspHI
AvaI AvaII AviII BalI BanI
BanII BbvCI BbvI BcnI BfaI
BmyI BpmI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DrdI DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
SapI Sau3AI Sau96I SbfI ScrFI
SfaNI SfcI SmlI Sse8387I StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII