################################## # NO CUT ENZYMES ( 57 enzymes) # ################################## AatII Acc65I AccIII AclI AgeI Aor51HI ApaI AscI Asp718I BamHI BbrPI BcgI BglII BseAI BsiWI BspEI BsrBI BstBI CpoI DraIII Eco47III FseI HpaI KpnI KspI MluI MroI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I StuI SwaI XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AhdI 3887 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AseI 513 ATTAAT AT^TAAT AsnI 513 ATTAAT AT^TAAT Asp700I 3336 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 3887 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 2878 CTCGGG C^[CT]CG[GA]G AviII 813 TGCGCA TGC^GCA AvrII 2564 CCTAGG C^CTAGG BanI 3215 GGCGCC G^G[CT][GA]CC BbeI 3215 GGCGCC GGCGC^C BbvCI 4223 GCTGAGG CCTCAGC(-5/-2) BclI 5076 TGATCA T^GATCA BglI 5563 GCCCGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 2564 CCTAGG C^CTAGG BlpI 1254 GCTGAGC GC^T[ACGT]AGC BpmI 2286 CTCCAG CTGGAG(16/14) Bpu1102I 1254 GCTGAGC GC^T[ACGT]AGC BsaAI 1326 TACGTG [CT]AC^GT[GA] BsaI 4618 GGTCTC GGTCTC(1/5) BsmBI 1532 CGTCTC CGTCTC(1/5) BsoBI 2878 CTCGGG C^[CT]CG[GA]G BspDI 1406 ATCGAT AT^CGAT BsrFI 1041 GCCGGC [GA]^CCGG[CT] BssHII 50 GCGCGC G^CGCGC BstEII 2935 GGTTACC G^GT[ACGT]ACC BstPI 2935 GGTTACC G^GT[ACGT]ACC CelII 1254 GCTGAGC GC^T[ACGT]AGC Cfr10I 1041 GCCGGC [GA]^CCGG[CT] ClaI 1406 ATCGAT AT^CGAT DrdI 151 GACCGGGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 78 CGGCCG C^GGCCG Eam1105I 3887 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 78 CGGCCG C^GGCCG Eco52I 78 CGGCCG C^GGCCG EcoNI 22 CCTGAGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 2935 GGTTACC G^GT[ACGT]ACC EcoRV 2316 GATATC GAT^ATC EcoT22I 4904 ATGCAT ATGCA^T FbaI 5076 TGATCA T^GATCA FspI 813 TGCGCA TGC^GCA HincII 2736 GTTGAC GT[CT]^[GA]AC HindII 2736 GTTGAC GT[CT]^[GA]AC KasI 3215 GGCGCC G^GCGCC MfeI 2194 CAATTG C^AATTG MspA1I 310 CAGCTG C[AC]G^C[GT]G MunI 2194 CAATTG C^AATTG NaeI 1041 GCCGGC GCC^GGC NarI 3215 GGCGCC GG^CGCC NdeI 2572 CATATG CA^TATG NgoMIV 1041 GCCGGC G^CCGGC NotI 77 GCGGCCGC GC^GGCCGC NsiI 4904 ATGCAT ATGCA^T PflMI 613 CCACGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 513 ATTAAT AT^TAAT PvuII 310 CAGCTG CAG^CTG SfoI 3215 GGCGCC GGC^GCC SphI 164 GCATGC GCATG^C Van91I 613 CCACGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 3336 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcyI 157 GGCGTC G[GA]^CG[CT]C 3215 GGCGCC AflII 2634 CTTAAG C^TTAAG 3201 CTTAAG Alw44I 788 GTGCAC G^TGCAC 5369 GTGCAC ApaLI 788 GTGCAC G^TGCAC 5369 GTGCAC BanII 2559 GAGCTC G[GA]GC[CT]^C 2846 GAGCTC BfrI 2634 CTTAAG C^TTAAG 3201 CTTAAG BsaHI 157 GGCGTC G[GA]^CG[CT]C 3215 GGCGCC BsaWI 2179 TCCGGT [AT]^CCGG[AT] 2785 TCCGGT BsiEI 78 CGGCCG CG[GA][CT]^CG 150 CGACCG BsmI 762 GAATGC GAATGC(1/-1) 1014 GAATGC Bsp1407I 4202 TGTACA T^GTACA 4836 TGTACA BspHI 1765 TCATGA T^CATGA 4139 TCATGA BsrGI 4202 TGTACA T^GTACA 4836 TGTACA Bst1107I 2399 GTATAC GTA^TAC 4591 GTATAC BstZ17I 2399 GTATAC GTA^TAC 4591 GTATAC DraI 651 TTTAAA TTT^AAA 2718 TTTAAA Hin1I 157 GGCGTC G[GA]^CG[CT]C 3215 GGCGCC NheI 890 GCTAGC G^CTAGC 5284 GCTAGC PpuMI 3269 AGGTCCT [GA]G^G[AT]CC[CT] 3885 AGGACCT RcaI 1765 TCATGA T^CATGA 4139 TCATGA SacI 2559 GAGCTC GAGCT^C 2846 GAGCTC ScaI 3457 AGTACT AGT^ACT 4951 AGTACT SspBI 4202 TGTACA T^GTACA 4836 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 119 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI AspHI AspI AvaII BalI BbsI BbvI BciVI BcnI BfaI BmyI BpuAI BsaBI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspLU11I BspMI BsrDI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflFI PleI PstI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XhoII