##################################
# NO CUT ENZYMES (   57 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AgeI        
Aor51HI     ApaI        AscI        Asp718I     BamHI       
BbrPI       BcgI        BglII       BseAI       BsiWI       
BspEI       BsrBI       BstBI       CpoI        DraIII      
Eco47III    FseI        HpaI        KpnI        KspI        
MluI        MroI        NruI        NspV        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshAI       Psp1406I    PvuI        RsrII       SacII       
SalI        SbfI        SexAI       SfiI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
StuI        SwaI        XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   59 enzymes) #
###################################

AhdI         3887  GACCTTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI          513  ATTAAT               AT^TAAT
AsnI          513  ATTAAT               AT^TAAT
Asp700I      3336  GAAAGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        3887  GACCTTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI         2878  CTCGGG               C^[CT]CG[GA]G
AviII         813  TGCGCA               TGC^GCA
AvrII        2564  CCTAGG               C^CTAGG
BanI         3215  GGCGCC               G^G[CT][GA]CC
BbeI         3215  GGCGCC               GGCGC^C
BbvCI        4223  GCTGAGG              CCTCAGC(-5/-2)
BclI         5076  TGATCA               T^GATCA
BglI         5563  GCCCGGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         2564  CCTAGG               C^CTAGG
BlpI         1254  GCTGAGC              GC^T[ACGT]AGC
BpmI         2286  CTCCAG               CTGGAG(16/14)
Bpu1102I     1254  GCTGAGC              GC^T[ACGT]AGC
BsaAI        1326  TACGTG               [CT]AC^GT[GA]
BsaI         4618  GGTCTC               GGTCTC(1/5)
BsmBI        1532  CGTCTC               CGTCTC(1/5)
BsoBI        2878  CTCGGG               C^[CT]CG[GA]G
BspDI        1406  ATCGAT               AT^CGAT
BsrFI        1041  GCCGGC               [GA]^CCGG[CT]
BssHII         50  GCGCGC               G^CGCGC
BstEII       2935  GGTTACC              G^GT[ACGT]ACC
BstPI        2935  GGTTACC              G^GT[ACGT]ACC
CelII        1254  GCTGAGC              GC^T[ACGT]AGC
Cfr10I       1041  GCCGGC               [GA]^CCGG[CT]
ClaI         1406  ATCGAT               AT^CGAT
DrdI          151  GACCGGGGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI           78  CGGCCG               C^GGCCG
Eam1105I     3887  GACCTTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI          78  CGGCCG               C^GGCCG
Eco52I         78  CGGCCG               C^GGCCG
EcoNI          22  CCTGAGGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      2935  GGTTACC              G^GT[ACGT]ACC
EcoRV        2316  GATATC               GAT^ATC
EcoT22I      4904  ATGCAT               ATGCA^T
FbaI         5076  TGATCA               T^GATCA
FspI          813  TGCGCA               TGC^GCA
HincII       2736  GTTGAC               GT[CT]^[GA]AC
HindII       2736  GTTGAC               GT[CT]^[GA]AC
KasI         3215  GGCGCC               G^GCGCC
MfeI         2194  CAATTG               C^AATTG
MspA1I        310  CAGCTG               C[AC]G^C[GT]G
MunI         2194  CAATTG               C^AATTG
NaeI         1041  GCCGGC               GCC^GGC
NarI         3215  GGCGCC               GG^CGCC
NdeI         2572  CATATG               CA^TATG
NgoMIV       1041  GCCGGC               G^CCGGC
NotI           77  GCGGCCGC             GC^GGCCGC
NsiI         4904  ATGCAT               ATGCA^T
PflMI         613  CCACGCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI         513  ATTAAT               AT^TAAT
PvuII         310  CAGCTG               CAG^CTG
SfoI         3215  GGCGCC               GGC^GCC
SphI          164  GCATGC               GCATG^C
Van91I        613  CCACGCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI         3336  GAAAGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcyI          157  GGCGTC               G[GA]^CG[CT]C
             3215  GGCGCC              
AflII        2634  CTTAAG               C^TTAAG
             3201  CTTAAG              
Alw44I        788  GTGCAC               G^TGCAC
             5369  GTGCAC              
ApaLI         788  GTGCAC               G^TGCAC
             5369  GTGCAC              
BanII        2559  GAGCTC               G[GA]GC[CT]^C
             2846  GAGCTC              
BfrI         2634  CTTAAG               C^TTAAG
             3201  CTTAAG              
BsaHI         157  GGCGTC               G[GA]^CG[CT]C
             3215  GGCGCC              
BsaWI        2179  TCCGGT               [AT]^CCGG[AT]
             2785  TCCGGT              
BsiEI          78  CGGCCG               CG[GA][CT]^CG
              150  CGACCG              
BsmI          762  GAATGC               GAATGC(1/-1)
             1014  GAATGC              
Bsp1407I     4202  TGTACA               T^GTACA
             4836  TGTACA              
BspHI        1765  TCATGA               T^CATGA
             4139  TCATGA              
BsrGI        4202  TGTACA               T^GTACA
             4836  TGTACA              
Bst1107I     2399  GTATAC               GTA^TAC
             4591  GTATAC              
BstZ17I      2399  GTATAC               GTA^TAC
             4591  GTATAC              
DraI          651  TTTAAA               TTT^AAA
             2718  TTTAAA              
Hin1I         157  GGCGTC               G[GA]^CG[CT]C
             3215  GGCGCC              
NheI          890  GCTAGC               G^CTAGC
             5284  GCTAGC              
PpuMI        3269  AGGTCCT              [GA]G^G[AT]CC[CT]
             3885  AGGACCT             
RcaI         1765  TCATGA               T^CATGA
             4139  TCATGA              
SacI         2559  GAGCTC               GAGCT^C
             2846  GAGCTC              
ScaI         3457  AGTACT               AGT^ACT
             4951  AGTACT              
SspBI        4202  TGTACA               T^GTACA
             4836  TGTACA              


#########################################
# 3 OR MORE CUT ENZYMES (  119 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AflIII      AluI        AlwI        AlwNI       AocI        
ApoI        AspHI       AspI        AvaII       BalI        
BbsI        BbvI        BciVI       BcnI        BfaI        
BmyI        BpuAI       BsaBI       BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BspLU11I    BspMI       BsrDI       
BsrI        BssKI       BssSI       BstAPI      BstF5I      
BstNI       BstUI       BstXI       BstYI       Bsu36I      
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        Eco81I      
EcoO109I    EcoRI       EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        HinP1I      HindIII     HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MamI        MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspI        MvaI        MvnI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PflFI       PleI        PstI        RsaI        
SapI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SspI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       Tth111I     TthHB8I     XhoII