##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Aor51HI ApaI AscI Asp718I BamHI
BbrPI BcgI BglII BseAI BsiWI
BspEI BsrBI BstBI CpoI DraIII
Eco47III FseI HpaI KpnI KspI
MluI MroI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SalI SbfI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
StuI SwaI XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AhdI 3887 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 513 ATTAAT AT^TAAT
AsnI 513 ATTAAT AT^TAAT
Asp700I 3336 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3887 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2878 CTCGGG C^[CT]CG[GA]G
AviII 813 TGCGCA TGC^GCA
AvrII 2564 CCTAGG C^CTAGG
BanI 3215 GGCGCC G^G[CT][GA]CC
BbeI 3215 GGCGCC GGCGC^C
BbvCI 4223 GCTGAGG CCTCAGC(-5/-2)
BclI 5076 TGATCA T^GATCA
BglI 5563 GCCCGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2564 CCTAGG C^CTAGG
BlpI 1254 GCTGAGC GC^T[ACGT]AGC
BpmI 2286 CTCCAG CTGGAG(16/14)
Bpu1102I 1254 GCTGAGC GC^T[ACGT]AGC
BsaAI 1326 TACGTG [CT]AC^GT[GA]
BsaI 4618 GGTCTC GGTCTC(1/5)
BsmBI 1532 CGTCTC CGTCTC(1/5)
BsoBI 2878 CTCGGG C^[CT]CG[GA]G
BspDI 1406 ATCGAT AT^CGAT
BsrFI 1041 GCCGGC [GA]^CCGG[CT]
BssHII 50 GCGCGC G^CGCGC
BstEII 2935 GGTTACC G^GT[ACGT]ACC
BstPI 2935 GGTTACC G^GT[ACGT]ACC
CelII 1254 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1041 GCCGGC [GA]^CCGG[CT]
ClaI 1406 ATCGAT AT^CGAT
DrdI 151 GACCGGGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 78 CGGCCG C^GGCCG
Eam1105I 3887 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 78 CGGCCG C^GGCCG
Eco52I 78 CGGCCG C^GGCCG
EcoNI 22 CCTGAGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2935 GGTTACC G^GT[ACGT]ACC
EcoRV 2316 GATATC GAT^ATC
EcoT22I 4904 ATGCAT ATGCA^T
FbaI 5076 TGATCA T^GATCA
FspI 813 TGCGCA TGC^GCA
HincII 2736 GTTGAC GT[CT]^[GA]AC
HindII 2736 GTTGAC GT[CT]^[GA]AC
KasI 3215 GGCGCC G^GCGCC
MfeI 2194 CAATTG C^AATTG
MspA1I 310 CAGCTG C[AC]G^C[GT]G
MunI 2194 CAATTG C^AATTG
NaeI 1041 GCCGGC GCC^GGC
NarI 3215 GGCGCC GG^CGCC
NdeI 2572 CATATG CA^TATG
NgoMIV 1041 GCCGGC G^CCGGC
NotI 77 GCGGCCGC GC^GGCCGC
NsiI 4904 ATGCAT ATGCA^T
PflMI 613 CCACGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 513 ATTAAT AT^TAAT
PvuII 310 CAGCTG CAG^CTG
SfoI 3215 GGCGCC GGC^GCC
SphI 164 GCATGC GCATG^C
Van91I 613 CCACGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 3336 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 157 GGCGTC G[GA]^CG[CT]C
3215 GGCGCC
AflII 2634 CTTAAG C^TTAAG
3201 CTTAAG
Alw44I 788 GTGCAC G^TGCAC
5369 GTGCAC
ApaLI 788 GTGCAC G^TGCAC
5369 GTGCAC
BanII 2559 GAGCTC G[GA]GC[CT]^C
2846 GAGCTC
BfrI 2634 CTTAAG C^TTAAG
3201 CTTAAG
BsaHI 157 GGCGTC G[GA]^CG[CT]C
3215 GGCGCC
BsaWI 2179 TCCGGT [AT]^CCGG[AT]
2785 TCCGGT
BsiEI 78 CGGCCG CG[GA][CT]^CG
150 CGACCG
BsmI 762 GAATGC GAATGC(1/-1)
1014 GAATGC
Bsp1407I 4202 TGTACA T^GTACA
4836 TGTACA
BspHI 1765 TCATGA T^CATGA
4139 TCATGA
BsrGI 4202 TGTACA T^GTACA
4836 TGTACA
Bst1107I 2399 GTATAC GTA^TAC
4591 GTATAC
BstZ17I 2399 GTATAC GTA^TAC
4591 GTATAC
DraI 651 TTTAAA TTT^AAA
2718 TTTAAA
Hin1I 157 GGCGTC G[GA]^CG[CT]C
3215 GGCGCC
NheI 890 GCTAGC G^CTAGC
5284 GCTAGC
PpuMI 3269 AGGTCCT [GA]G^G[AT]CC[CT]
3885 AGGACCT
RcaI 1765 TCATGA T^CATGA
4139 TCATGA
SacI 2559 GAGCTC GAGCT^C
2846 GAGCTC
ScaI 3457 AGTACT AGT^ACT
4951 AGTACT
SspBI 4202 TGTACA T^GTACA
4836 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI AspHI AspI AvaII BalI
BbsI BbvI BciVI BcnI BfaI
BmyI BpuAI BsaBI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspLU11I BspMI BsrDI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflFI PleI PstI RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XhoII