##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII Acc65I AccIII AgeI Aor51HI
AscI Asp718I AviII AvrII BlnI
BsaI BseAI BsiWI BsmBI BspDI
BspEI BsrBI BsrDI BsrFI BstEII
BstPI Cfr10I ClaI CpoI EagI
EclXI Eco47III Eco52I EcoO65I FseI
FspI KpnI KspI MluI MroI
NaeI NdeI NgoMIV NheI NotI
NruI PacI PinAI PmeI RsrII
SacII SalI SapI SbfI SexAI
SfiI SgrAI Sse8387I SspI SwaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AclI 3582 AACGTT AA^CGTT
AcyI 585 GGCGCC G[GA]^CG[CT]C
AhdI 124 GACATTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4064 GTGCAC G^TGCAC
AocI 4487 CCTTAGG CC^T[ACGT]AGG
ApaI 561 GGGCCC GGGCC^C
ApaLI 4064 GTGCAC G^TGCAC
AspEI 124 GACATTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 617 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 585 GGCGCC GGCGC^C
BbrPI 5776 CACGTG CAC^GTG
BcgI 764 GCACTGTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 5922 GTATCC GTATCC(6/5)
BlpI 702 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 702 GCTCAGC GC^T[ACGT]AGC
BsaHI 585 GGCGCC G[GA]^CG[CT]C
BsaWI 3664 TCCGGT [AT]^CCGG[AT]
BsmI 1328 GAATGC GAATGC(1/-1)
BstBI 541 TTCGAA TT^CGAA
BstXI 4851 CCACATAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 4487 CCTTAGG CC^T[ACGT]AGG
CelII 702 GCTCAGC GC^T[ACGT]AGC
DraI 4303 TTTAAA TTT^AAA
Eam1105I 124 GACATTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 4487 CCTTAGG CC^T[ACGT]AGG
EcoRV 2554 GATATC GAT^ATC
EcoT22I 4778 ATGCAT ATGCA^T
HgaI 621 GCGTC GACGC(5/10)
Hin1I 585 GGCGCC G[GA]^CG[CT]C
HpaI 386 GTTAAC GTT^AAC
KasI 585 GGCGCC G^GCGCC
NarI 585 GGCGCC GG^CGCC
NcoI 1876 CCATGG C^CATGG
NsiI 4778 ATGCAT ATGCA^T
NspV 541 TTCGAA TT^CGAA
PflFI 617 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 5776 CACGTG CAC^GTG
PmlI 5776 CACGTG CAC^GTG
Psp1406I 3582 AACGTT AA^CGTT
PvuI 4195 CGATCG CGAT^CG
PvuII 965 CAGCTG CAG^CTG
ScaI 4434 AGTACT AGT^ACT
SfoI 585 GGCGCC GGC^GCC
SfuI 541 TTCGAA TT^CGAA
SnaBI 3207 TACGTA TAC^GTA
Tth111I 617 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AflIII 4376 ACATGT A^C[GA][CT]GT
4986 ACATGT
BalI 5195 TGGCCA TGG^CCA
6417 TGGCCA
BamHI 5549 GGATCC G^GATCC
5633 GGATCC
BanI 585 GGCGCC G^G[CT][GA]CC
1659 GGTGCC
BbvCI 347 GCTGAGG CCTCAGC(-5/-2)
1108 GCTGAGG
BglI 2761 GCCTGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4536 GCCTCTATGGC
BsaBI 4081 GATTAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4777 GATGCATATC
BsiEI 4195 CGATCG CG[GA][CT]^CG
5623 CGACCG
BspHI 859 TCATGA T^CATGA
2049 TCATGA
BspLU11I 4376 ACATGT A^CATGT
4986 ACATGT
BssSI 4291 CTCGTG CACGAG(-5/-1)
6155 CTCGTG
BstAPI 955 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2702 GCAGGGCCTGC
DsaI 1876 CCATGG C^C[GA][CT]GG
5799 CCGTGG
EaeI 5195 TGGCCA [CT]^GGCC[GA]
6417 TGGCCA
EcoNI 2708 CCTGCATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4003 CCTGCCATAGG
HaeII 531 AGCGCC [GA]GCGC^[CT]
585 GGCGCC
HincII 386 GTTAAC GT[CT]^[GA]AC
2174 GTCAAC
HindII 386 GTTAAC GT[CT]^[GA]AC
2174 GTCAAC
MamI 4081 GATTAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4777 GATGCATATC
MfeI 1672 CAATTG C^AATTG
6413 CAATTG
MluNI 5195 TGGCCA TGG^CCA
6417 TGGCCA
MscI 5195 TGGCCA TGG^CCA
6417 TGGCCA
MspA1I 965 CAGCTG C[AC]G^C[GT]G
5592 CCGCTG
MunI 1672 CAATTG C^AATTG
6413 CAATTG
PaeR7I 1058 CTCGAG C^TCGAG
3014 CTCGAG
PshAI 680 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5522 GACTCTGGTC
RcaI 859 TCATGA T^CATGA
2049 TCATGA
SacI 236 GAGCTC GAGCT^C
5393 GAGCTC
SmaI 425 CCCGGG CCC^GGG
558 CCCGGG
SpeI 3598 ACTAGT A^CTAGT
3928 ACTAGT
SphI 2688 GCATGC GCATG^C
5699 GCATGC
XbaI 1759 TCTAGA T^CTAGA
2294 TCTAGA
XcmI 937 CCAGAGCATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4249 CCATCCTTCTGCTGG
XhoI 1058 CTCGAG C^TCGAG
3014 CTCGAG
XmaCI 425 CCCGGG C^CCGGG
558 CCCGGG
XmaI 425 CCCGGG C^CCGGG
558 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflII AluI AlwI AlwNI ApoI
AseI AsnI Asp700I AspHI AvaI
AvaII BanII BbsI BbvI BclI
BcnI BfaI BfrI BglII BmyI
BpmI BpuAI BsaAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrGI BsrI BssHII BssKI Bst1107I
BstF5I BstNI BstUI BstYI BstZ17I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DraIII DrdI EarI EcoO109I
EcoRI EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PshBI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmnI