################################## # NO CUT ENZYMES ( 55 enzymes) # ################################## Acc65I AclI AflII AgeI AscI AseI AsnI Asp718I AviII BclI BfrI BglII BsiEI BsiWI BssHII BstBI BstEII BstPI CpoI DraI EagI EclXI Eco52I EcoO65I EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI KspI NdeI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SfiI SfuI SgrAI SnaBI SpeI SspI SwaI XhoI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AatII 476 GACGTC GACGT^C AccIII 4677 TCCGGA T^CCGGA AhdI 395 GACGCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp700I 2199 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 395 GACGCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 3522 CCTAGG C^CTAGG BcgI 2237 GCAAGGAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 4009 GTATCC GTATCC(6/5) BlnI 3522 CCTAGG C^CTAGG BlpI 862 GCTGAGC GC^T[ACGT]AGC Bpu1102I 862 GCTGAGC GC^T[ACGT]AGC BsaBI 4093 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 4677 TCCGGA T^CCGGA BsmBI 2777 CGTCTC CGTCTC(1/5) BsmI 1596 GCATTC GAATGC(1/-1) BspDI 4913 ATCGAT AT^CGAT BspEI 4677 TCCGGA T^CCGGA BspHI 2613 TCATGA T^CATGA BsrFI 530 GCCGGC [GA]^CCGG[CT] Bst1107I 163 GTATAC GTA^TAC BstZ17I 163 GTATAC GTA^TAC CelII 862 GCTGAGC GC^T[ACGT]AGC Cfr10I 530 GCCGGC [GA]^CCGG[CT] ClaI 4913 ATCGAT AT^CGAT DrdI 3279 GACCGTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 395 GACGCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 2501 GAATTC G^AATTC MamI 4093 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1992 CAATTG C^AATTG MluI 1030 ACGCGT A^CGCGT MroI 4677 TCCGGA T^CCGGA MunI 1992 CAATTG C^AATTG NaeI 530 GCCGGC GCC^GGC NgoMIV 530 GCCGGC G^CCGGC NheI 787 GCTAGC G^CTAGC RcaI 2613 TCATGA T^CATGA SalI 2417 GTCGAC G^TCGAC SbfI 1389 CCTGCAGG CCTGCA^GG Sse8387I 1389 CCTGCAGG CCTGCA^GG XbaI 2505 TCTAGA T^CTAGA XmnI 2199 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AccII 1031 CGCG CG^CG 4515 CGCG AcsI 127 AAATTT [GA]^AATT[CT] 2501 GAATTC ApoI 127 AAATTT [GA]^AATT[CT] 2501 GAATTC AspI 1774 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC 4887 GACGAGGTC BamHI 1839 GGATCC G^GATCC 2685 GGATCC BglI 753 GCCAGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1359 GCCCCGCTGGC BsaWI 167 ACCGGA [AT]^CCGG[AT] 4677 TCCGGA BsgI 85 GTGCAG GTGCAG(16/14) 4769 GTGCAG BsrBI 202 GAGCGG CCGCTC(-3/-3) 865 GAGCGG BstUI 1031 CGCG CG^CG 4515 CGCG MvnI 1031 CGCG CG^CG 4515 CGCG PflFI 1774 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC 4887 GACGAGGTC PshAI 1894 GACCCTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1983 GACACATGTC SapI 4310 GAAGAGC GCTCTTC(1/4) 4628 GAAGAGC SexAI 892 ACCTGGT A^CC[AT]GGT 4341 ACCAGGT SphI 1257 GCATGC GCATG^C 4471 GCATGC Tth111I 1774 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC 4887 GACGAGGTC ######################################### # 3 OR MORE CUT ENZYMES ( 145 enzymes) # ######################################### AccI AciI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AspHI AvaI AvaII BalI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspLU11I BspMI BsrDI BsrGI BsrI BssKI BssSI BstAPI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco47III Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SfoI SmaI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI