##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
Acc65I AclI AflII AgeI AscI
AseI AsnI Asp718I AviII BclI
BfrI BglII BsiEI BsiWI BssHII
BstBI BstEII BstPI CpoI DraI
EagI EclXI Eco52I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KpnI KspI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshBI Psp1406I PvuI
RsrII SacII SfiI SfuI SgrAI
SnaBI SpeI SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 476 GACGTC GACGT^C
AccIII 4677 TCCGGA T^CCGGA
AhdI 395 GACGCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 2199 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 395 GACGCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3522 CCTAGG C^CTAGG
BcgI 2237 GCAAGGAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4009 GTATCC GTATCC(6/5)
BlnI 3522 CCTAGG C^CTAGG
BlpI 862 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 862 GCTGAGC GC^T[ACGT]AGC
BsaBI 4093 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 4677 TCCGGA T^CCGGA
BsmBI 2777 CGTCTC CGTCTC(1/5)
BsmI 1596 GCATTC GAATGC(1/-1)
BspDI 4913 ATCGAT AT^CGAT
BspEI 4677 TCCGGA T^CCGGA
BspHI 2613 TCATGA T^CATGA
BsrFI 530 GCCGGC [GA]^CCGG[CT]
Bst1107I 163 GTATAC GTA^TAC
BstZ17I 163 GTATAC GTA^TAC
CelII 862 GCTGAGC GC^T[ACGT]AGC
Cfr10I 530 GCCGGC [GA]^CCGG[CT]
ClaI 4913 ATCGAT AT^CGAT
DrdI 3279 GACCGTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 395 GACGCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2501 GAATTC G^AATTC
MamI 4093 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1992 CAATTG C^AATTG
MluI 1030 ACGCGT A^CGCGT
MroI 4677 TCCGGA T^CCGGA
MunI 1992 CAATTG C^AATTG
NaeI 530 GCCGGC GCC^GGC
NgoMIV 530 GCCGGC G^CCGGC
NheI 787 GCTAGC G^CTAGC
RcaI 2613 TCATGA T^CATGA
SalI 2417 GTCGAC G^TCGAC
SbfI 1389 CCTGCAGG CCTGCA^GG
Sse8387I 1389 CCTGCAGG CCTGCA^GG
XbaI 2505 TCTAGA T^CTAGA
XmnI 2199 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccII 1031 CGCG CG^CG
4515 CGCG
AcsI 127 AAATTT [GA]^AATT[CT]
2501 GAATTC
ApoI 127 AAATTT [GA]^AATT[CT]
2501 GAATTC
AspI 1774 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
4887 GACGAGGTC
BamHI 1839 GGATCC G^GATCC
2685 GGATCC
BglI 753 GCCAGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1359 GCCCCGCTGGC
BsaWI 167 ACCGGA [AT]^CCGG[AT]
4677 TCCGGA
BsgI 85 GTGCAG GTGCAG(16/14)
4769 GTGCAG
BsrBI 202 GAGCGG CCGCTC(-3/-3)
865 GAGCGG
BstUI 1031 CGCG CG^CG
4515 CGCG
MvnI 1031 CGCG CG^CG
4515 CGCG
PflFI 1774 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
4887 GACGAGGTC
PshAI 1894 GACCCTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1983 GACACATGTC
SapI 4310 GAAGAGC GCTCTTC(1/4)
4628 GAAGAGC
SexAI 892 ACCTGGT A^CC[AT]GGT
4341 ACCAGGT
SphI 1257 GCATGC GCATG^C
4471 GCATGC
Tth111I 1774 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
4887 GACGAGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 145 enzymes) #
#########################################
AccI AciI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbrPI BbsI BbvCI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspLU11I BspMI BsrDI
BsrGI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco47III Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SfoI SmaI SmlI SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI