################################## # NO CUT ENZYMES ( 48 enzymes) # ################################## AclI AhdI AocI Aor51HI AspEI AviII BciVI BsiWI Bsp1407I BspDI BspHI BspLU11I BspMI BsrGI Bsu36I ClaI DraI Eam1105I Eco47III Eco81I EcoRV EcoT22I FseI FspI HindIII HpaI KspI MluI NotI NruI NsiI PacI PmeI PshAI Psp1406I PvuI RcaI SacII SalI SbfI ScaI SfiI SgrAI SnaBI SphI Sse8387I SspBI SwaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AatII 4881 GACGTC GACGT^C Acc65I 5251 GGTACC G^GTACC AccIII 152 TCCGGA T^CCGGA AflII 3153 CTTAAG C^TTAAG AflIII 1967 ACACGT A^C[GA][CT]GT AgeI 942 ACCGGT A^CCGGT Alw44I 4928 GTGCAC G^TGCAC ApaLI 4928 GTGCAC G^TGCAC AscI 5217 GGCGCGCC GG^CGCGCC AseI 4484 ATTAAT AT^TAAT AsnI 4484 ATTAAT AT^TAAT Asp700I 2044 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 5251 GGTACC G^GTACC BbrPI 4621 CACGTG CAC^GTG BcgI 3659 CGAAGGGGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 3421 TGATCA T^GATCA BfrI 3153 CTTAAG C^TTAAG BglI 4110 GCCCTAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 4685 GCTTAGC GC^T[ACGT]AGC Bpu1102I 4685 GCTTAGC GC^T[ACGT]AGC BsaBI 51 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 152 TCCGGA T^CCGGA BspEI 152 TCCGGA T^CCGGA BsrDI 768 CATTGC GCAATG(2/0) BssHII 5218 GCGCGC G^CGCGC Bst1107I 1086 GTATAC GTA^TAC BstZ17I 1086 GTATAC GTA^TAC CelII 4685 GCTTAGC GC^T[ACGT]AGC EcoRI 3264 GAATTC G^AATTC FbaI 3421 TGATCA T^GATCA HincII 631 GTTGAC GT[CT]^[GA]AC HindII 631 GTTGAC GT[CT]^[GA]AC KpnI 5251 GGTACC GGTAC^C MamI 51 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 67 CAATTG C^AATTG MroI 152 TCCGGA T^CCGGA MunI 67 CAATTG C^AATTG NaeI 2543 GCCGGC GCC^GGC NdeI 1619 CATATG CA^TATG NgoMIV 2543 GCCGGC G^CCGGC NheI 4694 GCTAGC G^CTAGC NspI 1772 ACATGC [GA]CATG^[CT] PaeR7I 1009 CTCGAG C^TCGAG PinAI 942 ACCGGT A^CCGGT PmaCI 4621 CACGTG CAC^GTG PmlI 4621 CACGTG CAC^GTG PshBI 4484 ATTAAT AT^TAAT SapI 571 GCTCTTC GCTCTTC(1/4) SpeI 849 ACTAGT A^CTAGT XbaI 1746 TCTAGA T^CTAGA XhoI 1009 CTCGAG C^TCGAG XmnI 2044 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 1086 GTATAC GT^[AC][GT]AC 2623 GTAGAC AlwNI 3798 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG 4557 CAGGCCCTG BbeI 2346 GGCGCC GGCGC^C 3225 GGCGCC BbsI 405 GAAGAC GAAGAC(2/6) 2826 GTCTTC BbvCI 3199 CCTCAGC CCTCAGC(-5/-2) 3477 CCTCAGC BglII 1013 AGATCT A^GATCT 4250 AGATCT BpuAI 405 GAAGAC GAAGAC(2/6) 2826 GTCTTC BsiEI 691 CGGCCG CG[GA][CT]^CG 2681 CGGCCG BsmBI 2103 CGTCTC CGTCTC(1/5) 1320 GAGACG BsmI 2063 GAATGC GAATGC(1/-1) 4299 GAATGC BsrBI 2612 CCGCTC CCGCTC(-3/-3) 2651 CCGCTC BssSI 1798 CACGAG CACGAG(-5/-1) 1868 CACGAG BstAPI 763 GCAGCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3455 GCAGATCATGC BstBI 4386 TTCGAA TT^CGAA 5123 TTCGAA CpoI 923 CGGACCG CG^G[AT]CCG 939 CGGACCG DraIII 1798 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG 2529 CACCGTGTG EagI 691 CGGCCG C^GGCCG 2681 CGGCCG EclXI 691 CGGCCG C^GGCCG 2681 CGGCCG Eco52I 691 CGGCCG C^GGCCG 2681 CGGCCG HaeII 2346 GGCGCC [GA]GCGC^[CT] 3225 GGCGCC KasI 2346 GGCGCC G^GCGCC 3225 GGCGCC NarI 2346 GGCGCC GG^CGCC 3225 GGCGCC NspV 4386 TTCGAA TT^CGAA 5123 TTCGAA PstI 4084 CTGCAG CTGCA^G 4699 CTGCAG RsrII 923 CGGACCG CG^G[AT]CCG 939 CGGACCG SacI 220 GAGCTC GAGCT^C 3948 GAGCTC SfoI 2346 GGCGCC GGC^GCC 3225 GGCGCC SfuI 4386 TTCGAA TT^CGAA 5123 TTCGAA SmlI 1009 CTCGAG C^T[CT][GA]AG 3153 CTTAAG SspI 1637 AATATT AAT^ATT 2046 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 128 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI ApaI ApoI AspHI AspI AvaI AvaII AvrII BalI BamHI BanI BanII BbvI BcnI BfaI BlnI BmyI BpmI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EarI EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PflFI PflMI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmaI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII XmaCI XmaI