##################################
# NO CUT ENZYMES ( 48 enzymes) #
##################################
AclI AhdI AocI Aor51HI AspEI
AviII BciVI BsiWI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrGI Bsu36I
ClaI DraI Eam1105I Eco47III Eco81I
EcoRV EcoT22I FseI FspI HindIII
HpaI KspI MluI NotI NruI
NsiI PacI PmeI PshAI Psp1406I
PvuI RcaI SacII SalI SbfI
ScaI SfiI SgrAI SnaBI SphI
Sse8387I SspBI SwaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 4881 GACGTC GACGT^C
Acc65I 5251 GGTACC G^GTACC
AccIII 152 TCCGGA T^CCGGA
AflII 3153 CTTAAG C^TTAAG
AflIII 1967 ACACGT A^C[GA][CT]GT
AgeI 942 ACCGGT A^CCGGT
Alw44I 4928 GTGCAC G^TGCAC
ApaLI 4928 GTGCAC G^TGCAC
AscI 5217 GGCGCGCC GG^CGCGCC
AseI 4484 ATTAAT AT^TAAT
AsnI 4484 ATTAAT AT^TAAT
Asp700I 2044 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 5251 GGTACC G^GTACC
BbrPI 4621 CACGTG CAC^GTG
BcgI 3659 CGAAGGGGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3421 TGATCA T^GATCA
BfrI 3153 CTTAAG C^TTAAG
BglI 4110 GCCCTAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 4685 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 4685 GCTTAGC GC^T[ACGT]AGC
BsaBI 51 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 152 TCCGGA T^CCGGA
BspEI 152 TCCGGA T^CCGGA
BsrDI 768 CATTGC GCAATG(2/0)
BssHII 5218 GCGCGC G^CGCGC
Bst1107I 1086 GTATAC GTA^TAC
BstZ17I 1086 GTATAC GTA^TAC
CelII 4685 GCTTAGC GC^T[ACGT]AGC
EcoRI 3264 GAATTC G^AATTC
FbaI 3421 TGATCA T^GATCA
HincII 631 GTTGAC GT[CT]^[GA]AC
HindII 631 GTTGAC GT[CT]^[GA]AC
KpnI 5251 GGTACC GGTAC^C
MamI 51 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 67 CAATTG C^AATTG
MroI 152 TCCGGA T^CCGGA
MunI 67 CAATTG C^AATTG
NaeI 2543 GCCGGC GCC^GGC
NdeI 1619 CATATG CA^TATG
NgoMIV 2543 GCCGGC G^CCGGC
NheI 4694 GCTAGC G^CTAGC
NspI 1772 ACATGC [GA]CATG^[CT]
PaeR7I 1009 CTCGAG C^TCGAG
PinAI 942 ACCGGT A^CCGGT
PmaCI 4621 CACGTG CAC^GTG
PmlI 4621 CACGTG CAC^GTG
PshBI 4484 ATTAAT AT^TAAT
SapI 571 GCTCTTC GCTCTTC(1/4)
SpeI 849 ACTAGT A^CTAGT
XbaI 1746 TCTAGA T^CTAGA
XhoI 1009 CTCGAG C^TCGAG
XmnI 2044 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 1086 GTATAC GT^[AC][GT]AC
2623 GTAGAC
AlwNI 3798 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
4557 CAGGCCCTG
BbeI 2346 GGCGCC GGCGC^C
3225 GGCGCC
BbsI 405 GAAGAC GAAGAC(2/6)
2826 GTCTTC
BbvCI 3199 CCTCAGC CCTCAGC(-5/-2)
3477 CCTCAGC
BglII 1013 AGATCT A^GATCT
4250 AGATCT
BpuAI 405 GAAGAC GAAGAC(2/6)
2826 GTCTTC
BsiEI 691 CGGCCG CG[GA][CT]^CG
2681 CGGCCG
BsmBI 2103 CGTCTC CGTCTC(1/5)
1320 GAGACG
BsmI 2063 GAATGC GAATGC(1/-1)
4299 GAATGC
BsrBI 2612 CCGCTC CCGCTC(-3/-3)
2651 CCGCTC
BssSI 1798 CACGAG CACGAG(-5/-1)
1868 CACGAG
BstAPI 763 GCAGCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3455 GCAGATCATGC
BstBI 4386 TTCGAA TT^CGAA
5123 TTCGAA
CpoI 923 CGGACCG CG^G[AT]CCG
939 CGGACCG
DraIII 1798 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
2529 CACCGTGTG
EagI 691 CGGCCG C^GGCCG
2681 CGGCCG
EclXI 691 CGGCCG C^GGCCG
2681 CGGCCG
Eco52I 691 CGGCCG C^GGCCG
2681 CGGCCG
HaeII 2346 GGCGCC [GA]GCGC^[CT]
3225 GGCGCC
KasI 2346 GGCGCC G^GCGCC
3225 GGCGCC
NarI 2346 GGCGCC GG^CGCC
3225 GGCGCC
NspV 4386 TTCGAA TT^CGAA
5123 TTCGAA
PstI 4084 CTGCAG CTGCA^G
4699 CTGCAG
RsrII 923 CGGACCG CG^G[AT]CCG
939 CGGACCG
SacI 220 GAGCTC GAGCT^C
3948 GAGCTC
SfoI 2346 GGCGCC GGC^GCC
3225 GGCGCC
SfuI 4386 TTCGAA TT^CGAA
5123 TTCGAA
SmlI 1009 CTCGAG C^T[CT][GA]AG
3153 CTTAAG
SspI 1637 AATATT AAT^ATT
2046 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApaI ApoI AspHI
AspI AvaI AvaII AvrII BalI
BamHI BanI BanII BbvI BcnI
BfaI BlnI BmyI BpmI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DrdI DsaI EaeI EarI EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PflFI
PflMI PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmaI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII XmaCI XmaI