##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AclI AgeI AhdI Alw44I
AocI ApaLI AscI AspEI AspI
AviII BcgI BsaBI BsiEI BsiWI
BsmBI BspDI BsrBI BsrFI BssHII
Bst1107I BstAPI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoRV
FseI FspI HgaI HpaI MamI
MluI NaeI NdeI NgoMIV NheI
NotI NruI PacI PflFI PinAI
PmeI Psp1406I PvuI RsrII SalI
SapI SbfI ScaI SexAI SfiI
SgrAI SmaI Sse8387I StuI SwaI
Tth111I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 792 GGTACC G^GTACC
AccIII 2603 TCCGGA T^CCGGA
AcyI 1025 GGCGCC G[GA]^CG[CT]C
AlwNI 2146 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 798 AGCGCT AGC^GCT
ApaI 866 GGGCCC GGGCC^C
AseI 3648 ATTAAT AT^TAAT
AsnI 3648 ATTAAT AT^TAAT
Asp700I 3054 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 792 GGTACC G^GTACC
BamHI 1974 GGATCC G^GATCC
BbeI 1025 GGCGCC GGCGC^C
BclI 1551 TGATCA T^GATCA
BglI 68 GCCTTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1797 AGATCT A^GATCT
BsaHI 1025 GGCGCC G[GA]^CG[CT]C
BseAI 2603 TCCGGA T^CCGGA
BspEI 2603 TCCGGA T^CCGGA
BssSI 399 CACGAG CACGAG(-5/-1)
BstBI 2980 TTCGAA TT^CGAA
BstEII 2857 GGTAACC G^GT[ACGT]ACC
BstPI 2857 GGTAACC G^GT[ACGT]ACC
Eco47III 798 AGCGCT AGC^GCT
EcoO65I 2857 GGTAACC G^GT[ACGT]ACC
EcoRI 758 GAATTC G^AATTC
FauI 572 CCCGC CCCGC(4/6)
FbaI 1551 TGATCA T^GATCA
Hin1I 1025 GGCGCC G[GA]^CG[CT]C
HincII 2196 GTCAAC GT[CT]^[GA]AC
HindII 2196 GTCAAC GT[CT]^[GA]AC
KasI 1025 GGCGCC G^GCGCC
KpnI 792 GGTACC GGTAC^C
KspI 1858 CCGCGG CCGC^GG
MfeI 366 CAATTG C^AATTG
MroI 2603 TCCGGA T^CCGGA
MunI 366 CAATTG C^AATTG
NarI 1025 GGCGCC GG^CGCC
NspV 2980 TTCGAA TT^CGAA
PaeR7I 1746 CTCGAG C^TCGAG
PshAI 131 GACATGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3648 ATTAAT AT^TAAT
PvuII 538 CAGCTG CAG^CTG
SacI 775 GAGCTC GAGCT^C
SacII 1858 CCGCGG CCGC^GG
SfoI 1025 GGCGCC GGC^GCC
SfuI 2980 TTCGAA TT^CGAA
SpeI 3270 ACTAGT A^CTAGT
SphI 2997 GCATGC GCATG^C
SspI 91 AATATT AAT^ATT
XhoI 1746 CTCGAG C^TCGAG
XmnI 3054 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 176 GTCTAC GT^[AC][GT]AC
1846 GTCTAC
AccII 410 CGCG CG^CG
1859 CGCG
AcsI 758 GAATTC [GA]^AATT[CT]
2949 AAATTT
AflII 919 CTTAAG C^TTAAG
2130 CTTAAG
ApoI 758 GAATTC [GA]^AATT[CT]
2949 AAATTT
AvaI 1746 CTCGAG C^[CT]CG[GA]G
2971 CTCGGG
AvrII 181 CCTAGG C^CTAGG
3768 CCTAGG
BbrPI 1016 CACGTG CAC^GTG
2626 CACGTG
BbsI 1407 GAAGAC GAAGAC(2/6)
3999 GTCTTC
BbvCI 247 CCTCAGC CCTCAGC(-5/-2)
3148 GCTGAGG
BcnI 706 CCCGG CC^[GC]GG
1777 CCGGG
BfrI 919 CTTAAG C^TTAAG
2130 CTTAAG
BlnI 181 CCTAGG C^CTAGG
3768 CCTAGG
BlpI 1991 GCTTAGC GC^T[ACGT]AGC
3851 GCTCAGC
Bpu1102I 1991 GCTTAGC GC^T[ACGT]AGC
3851 GCTCAGC
BpuAI 1407 GAAGAC GAAGAC(2/6)
3999 GTCTTC
BsaI 2 GAGACC GGTCTC(1/5)
2002 GGTCTC
BsaWI 782 ACCGGA [AT]^CCGG[AT]
2603 TCCGGA
BsoBI 1746 CTCGAG C^[CT]CG[GA]G
2971 CTCGGG
Bsp1407I 2916 TGTACA T^GTACA
4227 TGTACA
BspMI 2672 GCAGGT ACCTGC(4/8)
3122 GCAGGT
BsrGI 2916 TGTACA T^GTACA
4227 TGTACA
BstUI 410 CGCG CG^CG
1859 CGCG
CelII 1991 GCTTAGC GC^T[ACGT]AGC
3851 GCTCAGC
EcoNI 1117 CCTCTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1896 CCTAGTTTAGG
HindIII 1073 AAGCTT A^AGCTT
3055 AAGCTT
MspA1I 538 CAGCTG C[AC]G^C[GT]G
1858 CCGCGG
MvnI 410 CGCG CG^CG
1859 CGCG
NciI 706 CCCGG CC^[GC]GG
1777 CCGGG
PflMI 471 CCATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
491 CCACACTATGG
PmaCI 1016 CACGTG CAC^GTG
2626 CACGTG
PmlI 1016 CACGTG CAC^GTG
2626 CACGTG
SnaBI 1174 TACGTA TAC^GTA
2226 TACGTA
SspBI 2916 TGTACA T^GTACA
4227 TGTACA
Van91I 471 CCATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
491 CCACACTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AspHI AvaII BalI BanI BanII
BbvI BciVI BfaI BmyI BpmI
BsaAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspHI BspLU11I BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspI MvaI MwoI
NcoI NdeII NlaIII NlaIV NsiI
NspI PleI PpuMI PstI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII