################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## AatII AclI AgeI AhdI Alw44I AocI ApaLI AscI AspEI AspI AviII BcgI BsaBI BsiEI BsiWI BsmBI BspDI BsrBI BsrFI BssHII Bst1107I BstAPI BstZ17I Bsu36I Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoRV FseI FspI HgaI HpaI MamI MluI NaeI NdeI NgoMIV NheI NotI NruI PacI PflFI PinAI PmeI Psp1406I PvuI RsrII SalI SapI SbfI ScaI SexAI SfiI SgrAI SmaI Sse8387I StuI SwaI Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### Acc65I 792 GGTACC G^GTACC AccIII 2603 TCCGGA T^CCGGA AcyI 1025 GGCGCC G[GA]^CG[CT]C AlwNI 2146 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 798 AGCGCT AGC^GCT ApaI 866 GGGCCC GGGCC^C AseI 3648 ATTAAT AT^TAAT AsnI 3648 ATTAAT AT^TAAT Asp700I 3054 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 792 GGTACC G^GTACC BamHI 1974 GGATCC G^GATCC BbeI 1025 GGCGCC GGCGC^C BclI 1551 TGATCA T^GATCA BglI 68 GCCTTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1797 AGATCT A^GATCT BsaHI 1025 GGCGCC G[GA]^CG[CT]C BseAI 2603 TCCGGA T^CCGGA BspEI 2603 TCCGGA T^CCGGA BssSI 399 CACGAG CACGAG(-5/-1) BstBI 2980 TTCGAA TT^CGAA BstEII 2857 GGTAACC G^GT[ACGT]ACC BstPI 2857 GGTAACC G^GT[ACGT]ACC Eco47III 798 AGCGCT AGC^GCT EcoO65I 2857 GGTAACC G^GT[ACGT]ACC EcoRI 758 GAATTC G^AATTC FauI 572 CCCGC CCCGC(4/6) FbaI 1551 TGATCA T^GATCA Hin1I 1025 GGCGCC G[GA]^CG[CT]C HincII 2196 GTCAAC GT[CT]^[GA]AC HindII 2196 GTCAAC GT[CT]^[GA]AC KasI 1025 GGCGCC G^GCGCC KpnI 792 GGTACC GGTAC^C KspI 1858 CCGCGG CCGC^GG MfeI 366 CAATTG C^AATTG MroI 2603 TCCGGA T^CCGGA MunI 366 CAATTG C^AATTG NarI 1025 GGCGCC GG^CGCC NspV 2980 TTCGAA TT^CGAA PaeR7I 1746 CTCGAG C^TCGAG PshAI 131 GACATGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 3648 ATTAAT AT^TAAT PvuII 538 CAGCTG CAG^CTG SacI 775 GAGCTC GAGCT^C SacII 1858 CCGCGG CCGC^GG SfoI 1025 GGCGCC GGC^GCC SfuI 2980 TTCGAA TT^CGAA SpeI 3270 ACTAGT A^CTAGT SphI 2997 GCATGC GCATG^C SspI 91 AATATT AAT^ATT XhoI 1746 CTCGAG C^TCGAG XmnI 3054 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AccI 176 GTCTAC GT^[AC][GT]AC 1846 GTCTAC AccII 410 CGCG CG^CG 1859 CGCG AcsI 758 GAATTC [GA]^AATT[CT] 2949 AAATTT AflII 919 CTTAAG C^TTAAG 2130 CTTAAG ApoI 758 GAATTC [GA]^AATT[CT] 2949 AAATTT AvaI 1746 CTCGAG C^[CT]CG[GA]G 2971 CTCGGG AvrII 181 CCTAGG C^CTAGG 3768 CCTAGG BbrPI 1016 CACGTG CAC^GTG 2626 CACGTG BbsI 1407 GAAGAC GAAGAC(2/6) 3999 GTCTTC BbvCI 247 CCTCAGC CCTCAGC(-5/-2) 3148 GCTGAGG BcnI 706 CCCGG CC^[GC]GG 1777 CCGGG BfrI 919 CTTAAG C^TTAAG 2130 CTTAAG BlnI 181 CCTAGG C^CTAGG 3768 CCTAGG BlpI 1991 GCTTAGC GC^T[ACGT]AGC 3851 GCTCAGC Bpu1102I 1991 GCTTAGC GC^T[ACGT]AGC 3851 GCTCAGC BpuAI 1407 GAAGAC GAAGAC(2/6) 3999 GTCTTC BsaI 2 GAGACC GGTCTC(1/5) 2002 GGTCTC BsaWI 782 ACCGGA [AT]^CCGG[AT] 2603 TCCGGA BsoBI 1746 CTCGAG C^[CT]CG[GA]G 2971 CTCGGG Bsp1407I 2916 TGTACA T^GTACA 4227 TGTACA BspMI 2672 GCAGGT ACCTGC(4/8) 3122 GCAGGT BsrGI 2916 TGTACA T^GTACA 4227 TGTACA BstUI 410 CGCG CG^CG 1859 CGCG CelII 1991 GCTTAGC GC^T[ACGT]AGC 3851 GCTCAGC EcoNI 1117 CCTCTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1896 CCTAGTTTAGG HindIII 1073 AAGCTT A^AGCTT 3055 AAGCTT MspA1I 538 CAGCTG C[AC]G^C[GT]G 1858 CCGCGG MvnI 410 CGCG CG^CG 1859 CGCG NciI 706 CCCGG CC^[GC]GG 1777 CCGGG PflMI 471 CCATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 491 CCACACTATGG PmaCI 1016 CACGTG CAC^GTG 2626 CACGTG PmlI 1016 CACGTG CAC^GTG 2626 CACGTG SnaBI 1174 TACGTA TAC^GTA 2226 TACGTA SspBI 2916 TGTACA T^GTACA 4227 TGTACA Van91I 471 CCATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 491 CCACACTATGG ######################################### # 3 OR MORE CUT ENZYMES ( 103 enzymes) # ######################################### AciI AfaI AflIII AluI AlwI AspHI AvaII BalI BanI BanII BbvI BciVI BfaI BmyI BpmI BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspHI BspLU11I BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspI MvaI MwoI NcoI NdeII NlaIII NlaIV NsiI NspI PleI PpuMI PstI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII