##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
Acc65I AccI AccIII AclI AflIII
AgeI AocI AscI Asp718I AviII
BbrPI BcgI BglI BsaAI BseAI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssHII Bst1107I BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraIII Eco81I EcoO65I FseI FspI
HpaI KpnI KspI MfeI MluI
MroI MunI NotI NruI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI PvuII RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SgrAI SnaBI Sse8387I SspBI SwaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AhdI 2237 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 2237 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 480 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4409 CCTAGG C^CTAGG
BamHI 4379 GGATCC G^GATCC
BbvCI 1428 GCTGAGG CCTCAGC(-5/-2)
BciVI 2043 GGATAC GTATCC(6/5)
BglII 877 AGATCT A^GATCT
BlnI 4409 CCTAGG C^CTAGG
BsiEI 1438 CGGCCG CG[GA][CT]^CG
BsmI 1150 GAATGC GAATGC(1/-1)
BssSI 2223 CACGAG CACGAG(-5/-1)
BstBI 380 TTCGAA TT^CGAA
DrdI 1770 GACAAGACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1438 CGGCCG C^GGCCG
Eam1105I 2237 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1438 CGGCCG C^GGCCG
Eco52I 1438 CGGCCG C^GGCCG
EcoNI 1341 CCTGTATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3277 GAATTC G^AATTC
HincII 4209 GTCAAC GT[CT]^[GA]AC
HindII 4209 GTCAAC GT[CT]^[GA]AC
NaeI 1005 GCCGGC GCC^GGC
NcoI 4071 CCATGG C^CATGG
NdeI 4084 CATATG CA^TATG
NgoMIV 1005 GCCGGC G^CCGGC
NheI 4818 GCTAGC G^CTAGC
NspV 380 TTCGAA TT^CGAA
PaeR7I 24 CTCGAG C^TCGAG
PflFI 480 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 4425 CCATCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 2155 CTGCAG CTGCA^G
SfuI 380 TTCGAA TT^CGAA
SmaI 150 CCCGGG CCC^GGG
SpeI 2593 ACTAGT A^CTAGT
SphI 583 GCATGC GCATG^C
SspI 3396 AATATT AAT^ATT
Tth111I 480 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 4425 CCATCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 24 CTCGAG C^TCGAG
XmaCI 150 CCCGGG C^CCGGG
XmaI 150 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AatII 483 GACGTC GACGT^C
1197 GACGTC
AccII 261 CGCG CG^CG
1084 CGCG
Alw44I 3005 GTGCAC G^TGCAC
4785 GTGCAC
Aor51HI 489 AGCGCT AGC^GCT
2504 AGCGCT
ApaI 154 GGGCCC GGGCC^C
292 GGGCCC
ApaLI 3005 GTGCAC G^TGCAC
4785 GTGCAC
AseI 2617 ATTAAT AT^TAAT
3900 ATTAAT
AsnI 2617 ATTAAT AT^TAAT
3900 ATTAAT
BbsI 2265 GAAGAC GAAGAC(2/6)
4739 GTCTTC
BclI 1599 TGATCA T^GATCA
3241 TGATCA
BpuAI 2265 GAAGAC GAAGAC(2/6)
4739 GTCTTC
BsaWI 310 ACCGGA [AT]^CCGG[AT]
1463 ACCGGA
BsmBI 643 GAGACG CGTCTC(1/5)
2000 GAGACG
BspMI 3644 ACCTGC ACCTGC(4/8)
3016 GCAGGT
BstAPI 540 GCAGGGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
766 GCATCCACTGC
BstUI 261 CGCG CG^CG
1084 CGCG
DraI 3512 TTTAAA TTT^AAA
4335 TTTAAA
DsaI 1556 CCGTGG C^C[GA][CT]GG
4071 CCATGG
Eco47III 489 AGCGCT AGC^GCT
2504 AGCGCT
EcoRV 3778 GATATC GAT^ATC
4764 GATATC
EcoT22I 2185 ATGCAT ATGCA^T
4081 ATGCAT
FbaI 1599 TGATCA T^GATCA
3241 TGATCA
HindIII 1651 AAGCTT A^AGCTT
4459 AAGCTT
MvnI 261 CGCG CG^CG
1084 CGCG
NsiI 2185 ATGCAT ATGCA^T
4081 ATGCAT
PacI 2406 TTAATTAA TTAAT^TAA
2614 TTAATTAA
PshBI 2617 ATTAAT AT^TAAT
3900 ATTAAT
StuI 2997 AGGCCT AGG^CCT
4403 AGGCCT
TaqI 25 TCGA T^CGA
381 TCGA
TthHB8I 25 TCGA T^CGA
381 TCGA
XbaI 3656 TCTAGA T^CTAGA
4137 TCTAGA
XcmI 308 CCACCGGAGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1079 CCAGCCGCGTGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbeI BbvI BcnI BfaI
BfrI BlpI BmyI BpmI Bpu1102I
BsaBI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrBI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NarI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII XmnI