################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## Acc65I AccI AccIII AclI AflIII AgeI AocI AscI Asp718I AviII BbrPI BcgI BglI BsaAI BseAI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII Bst1107I BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraIII Eco81I EcoO65I FseI FspI HpaI KpnI KspI MfeI MluI MroI MunI NotI NruI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SgrAI SnaBI Sse8387I SspBI SwaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AhdI 2237 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 2237 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 480 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 4409 CCTAGG C^CTAGG BamHI 4379 GGATCC G^GATCC BbvCI 1428 GCTGAGG CCTCAGC(-5/-2) BciVI 2043 GGATAC GTATCC(6/5) BglII 877 AGATCT A^GATCT BlnI 4409 CCTAGG C^CTAGG BsiEI 1438 CGGCCG CG[GA][CT]^CG BsmI 1150 GAATGC GAATGC(1/-1) BssSI 2223 CACGAG CACGAG(-5/-1) BstBI 380 TTCGAA TT^CGAA DrdI 1770 GACAAGACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1438 CGGCCG C^GGCCG Eam1105I 2237 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 1438 CGGCCG C^GGCCG Eco52I 1438 CGGCCG C^GGCCG EcoNI 1341 CCTGTATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 3277 GAATTC G^AATTC HincII 4209 GTCAAC GT[CT]^[GA]AC HindII 4209 GTCAAC GT[CT]^[GA]AC NaeI 1005 GCCGGC GCC^GGC NcoI 4071 CCATGG C^CATGG NdeI 4084 CATATG CA^TATG NgoMIV 1005 GCCGGC G^CCGGC NheI 4818 GCTAGC G^CTAGC NspV 380 TTCGAA TT^CGAA PaeR7I 24 CTCGAG C^TCGAG PflFI 480 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 4425 CCATCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 2155 CTGCAG CTGCA^G SfuI 380 TTCGAA TT^CGAA SmaI 150 CCCGGG CCC^GGG SpeI 2593 ACTAGT A^CTAGT SphI 583 GCATGC GCATG^C SspI 3396 AATATT AAT^ATT Tth111I 480 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 4425 CCATCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 24 CTCGAG C^TCGAG XmaCI 150 CCCGGG C^CCGGG XmaI 150 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AatII 483 GACGTC GACGT^C 1197 GACGTC AccII 261 CGCG CG^CG 1084 CGCG Alw44I 3005 GTGCAC G^TGCAC 4785 GTGCAC Aor51HI 489 AGCGCT AGC^GCT 2504 AGCGCT ApaI 154 GGGCCC GGGCC^C 292 GGGCCC ApaLI 3005 GTGCAC G^TGCAC 4785 GTGCAC AseI 2617 ATTAAT AT^TAAT 3900 ATTAAT AsnI 2617 ATTAAT AT^TAAT 3900 ATTAAT BbsI 2265 GAAGAC GAAGAC(2/6) 4739 GTCTTC BclI 1599 TGATCA T^GATCA 3241 TGATCA BpuAI 2265 GAAGAC GAAGAC(2/6) 4739 GTCTTC BsaWI 310 ACCGGA [AT]^CCGG[AT] 1463 ACCGGA BsmBI 643 GAGACG CGTCTC(1/5) 2000 GAGACG BspMI 3644 ACCTGC ACCTGC(4/8) 3016 GCAGGT BstAPI 540 GCAGGGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 766 GCATCCACTGC BstUI 261 CGCG CG^CG 1084 CGCG DraI 3512 TTTAAA TTT^AAA 4335 TTTAAA DsaI 1556 CCGTGG C^C[GA][CT]GG 4071 CCATGG Eco47III 489 AGCGCT AGC^GCT 2504 AGCGCT EcoRV 3778 GATATC GAT^ATC 4764 GATATC EcoT22I 2185 ATGCAT ATGCA^T 4081 ATGCAT FbaI 1599 TGATCA T^GATCA 3241 TGATCA HindIII 1651 AAGCTT A^AGCTT 4459 AAGCTT MvnI 261 CGCG CG^CG 1084 CGCG NsiI 2185 ATGCAT ATGCA^T 4081 ATGCAT PacI 2406 TTAATTAA TTAAT^TAA 2614 TTAATTAA PshBI 2617 ATTAAT AT^TAAT 3900 ATTAAT StuI 2997 AGGCCT AGG^CCT 4403 AGGCCT TaqI 25 TCGA T^CGA 381 TCGA TthHB8I 25 TCGA T^CGA 381 TCGA XbaI 3656 TCTAGA T^CTAGA 4137 TCTAGA XcmI 308 CCACCGGAGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1079 CCAGCCGCGTGATGG ######################################### # 3 OR MORE CUT ENZYMES ( 119 enzymes) # ######################################### AciI AcsI AcyI AfaI AflII AluI AlwI AlwNI ApoI Asp700I AspHI AvaI AvaII BalI BanI BanII BbeI BbvI BcnI BfaI BfrI BlpI BmyI BpmI Bpu1102I BsaBI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NarI NciI NdeII NlaIII NlaIV NspI PleI PpuMI RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII XmnI