##################################
# NO CUT ENZYMES (   50 enzymes) #
##################################

AccIII      AgeI        AhdI        Aor51HI     AscI        
AseI        AsnI        AspEI       AvrII       BamHI       
BcgI        BlnI        BsaWI       BseAI       BsiWI       
BspDI       BspEI       BspHI       BssHII      Bst1107I    
BstBI       BstZ17I     ClaI        CpoI        Eam1105I    
Eco47III    EcoRV       FseI        MluI        MroI        
NdeI        NotI        NruI        NspV        PinAI       
PmeI        PpuMI       PshBI       RcaI        RsrII       
SacI        SalI        SbfI        ScaI        SexAI       
SfuI        SgrAI       SphI        Sse8387I    SwaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AclI         2616  AACGTT               AA^CGTT
Alw44I       1524  GTGCAC               G^TGCAC
ApaI         1412  GGGCCC               GGGCC^C
ApaLI        1524  GTGCAC               G^TGCAC
AspI         1720  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         405  TGCGCA               TGC^GCA
BanII        1412  GGGCCC               G[GA]GC[CT]^C
BbeI          905  GGCGCC               GGCGC^C
BbrPI         767  CACGTG               CAC^GTG
BclI         3921  TGATCA               T^GATCA
BspMI        5587  ACCTGC               ACCTGC(4/8)
BssSI        1155  CTCGTG               CACGAG(-5/-1)
BstEII       3899  GGTTACC              G^GT[ACGT]ACC
BstPI        3899  GGTTACC              G^GT[ACGT]ACC
DraIII       4163  CACAATGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI        5640  CCTGAAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      3899  GGTTACC              G^GT[ACGT]ACC
EcoRI        5802  GAATTC               G^AATTC
EcoT22I      3268  ATGCAT               ATGCA^T
FbaI         3921  TGATCA               T^GATCA
FspI          405  TGCGCA               TGC^GCA
HindIII      2275  AAGCTT               A^AGCTT
KasI          905  GGCGCC               G^GCGCC
KspI         3687  CCGCGG               CCGC^GG
MfeI         2422  CAATTG               C^AATTG
MunI         2422  CAATTG               C^AATTG
NaeI         5182  GCCGGC               GCC^GGC
NarI          905  GGCGCC               GG^CGCC
NgoMIV       5182  GCCGGC               G^CCGGC
NheI         2019  GCTAGC               G^CTAGC
NsiI         3268  ATGCAT               ATGCA^T
PacI         2581  TTAATTAA             TTAAT^TAA
PaeR7I       1812  CTCGAG               C^TCGAG
PflFI        1720  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         767  CACGTG               CAC^GTG
PmlI          767  CACGTG               CAC^GTG
PshAI        5101  GACCACAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     2616  AACGTT               AA^CGTT
PvuI         3222  CGATCG               CGAT^CG
SacII        3687  CCGCGG               CCGC^GG
SapI          508  GCTCTTC              GCTCTTC(1/4)
SfiI         3311  GGCCAGTCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          905  GGCGCC               GGC^GCC
SnaBI        3414  TACGTA               TAC^GTA
SpeI         2267  ACTAGT               A^CTAGT
Tth111I      1720  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         4087  TCTAGA               T^CTAGA
XhoI         1812  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   46 enzymes) #
###################################

AatII         433  GACGTC               GACGT^C
             5707  GACGTC              
Acc65I       2490  GGTACC               G^GTACC
             4191  GGTACC              
AccII        3220  CGCG                 CG^CG
             3688  CGCG                
AflII        3110  CTTAAG               C^TTAAG
             3149  CTTAAG              
AlwNI        3363  CAGGCACTG            CAG[ACGT][ACGT][ACGT]^CTG
             3654  CAGTACCTG           
AocI         1201  CCTGAGG              CC^T[ACGT]AGG
             5283  CCTGAGG             
Asp718I      2490  GGTACC               G^GTACC
             4191  GGTACC              
BalI          559  TGGCCA               TGG^CCA
             1325  TGGCCA              
BfrI         3110  CTTAAG               C^TTAAG
             3149  CTTAAG              
BglI         2451  GCCACACGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             3312  GCCAGTCAGGC         
BlpI          956  GCTGAGC              GC^T[ACGT]AGC
             1341  GCTCAGC             
Bpu1102I      956  GCTGAGC              GC^T[ACGT]AGC
             1341  GCTCAGC             
BsaAI         767  CACGTG               [CT]AC^GT[GA]
             3414  TACGTA              
BsaBI        3916  GATTTTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4647  GATCTGCATC          
BsaI         2673  GAGACC               GGTCTC(1/5)
             3522  GAGACC              
BsrFI        2487  GCCGGT               [GA]^CCGG[CT]
             5182  GCCGGC              
BstAPI        539  GCATACTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3362  GCAGGCACTGC         
BstUI        3220  CGCG                 CG^CG
             3688  CGCG                
BstXI         999  CCACTGTTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2421  CCAATTGTGTGG        
Bsu36I       1201  CCTGAGG              CC^T[ACGT]AGG
             5283  CCTGAGG             
CelII         956  GCTGAGC              GC^T[ACGT]AGC
             1341  GCTCAGC             
Cfr10I       2487  GCCGGT               [GA]^CCGG[CT]
             5182  GCCGGC              
DrdI          476  GACAAACACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3625  GACCTTGGAGTC        
EagI           72  CGGCCG               C^GGCCG
              125  CGGCCG              
EclXI          72  CGGCCG               C^GGCCG
              125  CGGCCG              
Eco52I         72  CGGCCG               C^GGCCG
              125  CGGCCG              
Eco81I       1201  CCTGAGG              CC^T[ACGT]AGG
             5283  CCTGAGG             
HaeII         905  GGCGCC               [GA]GCGC^[CT]
             1317  AGCGCC              
HpaI          608  GTTAAC               GTT^AAC
             4058  GTTAAC              
KpnI         2490  GGTACC               GGTAC^C
             4191  GGTACC              
MamI         3916  GATTTTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4647  GATCTGCATC          
MluNI         559  TGGCCA               TGG^CCA
             1325  TGGCCA              
MscI          559  TGGCCA               TGG^CCA
             1325  TGGCCA              
MslI         4049  CACAGCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             5133  CATTTCCATG          
MvnI         3220  CGCG                 CG^CG
             3688  CGCG                
NcoI          562  CCATGG               C^CATGG
             1898  CCATGG              
PflMI        2680  CCATCAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4892  CCAAATACTGG         
PstI          185  CTGCAG               CTGCA^G
              379  CTGCAG              
PvuII          98  CAGCTG               CAG^CTG
             2684  CAGCTG              
SmaI           61  CCCGGG               CCC^GGG
             1710  CCCGGG              
SspI         4227  AATATT               AAT^ATT
             4913  AATATT              
StuI          413  AGGCCT               AGG^CCT
             5613  AGGCCT              
Van91I       2680  CCATCAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4892  CCAAATACTGG         
XcmI         1970  CCAGACTTTCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             5190  CCAAGGGGCAGATGG     
XmaCI          61  CCCGGG               C^CCGGG
             1710  CCCGGG              
XmaI           61  CCCGGG               C^CCGGG
             1710  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  114 enzymes) #
#########################################

AccI        AciI        AcsI        AcyI        AfaI        
AflIII      AluI        AlwI        ApoI        Asp700I     
AspHI       AvaI        AvaII       BanI        BbsI        
BbvCI       BbvI        BciVI       BcnI        BfaI        
BglII       BmyI        BpmI        BpuAI       BsaHI       
BsaJI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspLU11I    
BsrBI       BsrDI       BsrGI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraI        DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PleI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SspBI       StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       XmnI