##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
AccIII AgeI AhdI Aor51HI AscI
AseI AsnI AspEI AvrII BamHI
BcgI BlnI BsaWI BseAI BsiWI
BspDI BspEI BspHI BssHII Bst1107I
BstBI BstZ17I ClaI CpoI Eam1105I
Eco47III EcoRV FseI MluI MroI
NdeI NotI NruI NspV PinAI
PmeI PpuMI PshBI RcaI RsrII
SacI SalI SbfI ScaI SexAI
SfuI SgrAI SphI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AclI 2616 AACGTT AA^CGTT
Alw44I 1524 GTGCAC G^TGCAC
ApaI 1412 GGGCCC GGGCC^C
ApaLI 1524 GTGCAC G^TGCAC
AspI 1720 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 405 TGCGCA TGC^GCA
BanII 1412 GGGCCC G[GA]GC[CT]^C
BbeI 905 GGCGCC GGCGC^C
BbrPI 767 CACGTG CAC^GTG
BclI 3921 TGATCA T^GATCA
BspMI 5587 ACCTGC ACCTGC(4/8)
BssSI 1155 CTCGTG CACGAG(-5/-1)
BstEII 3899 GGTTACC G^GT[ACGT]ACC
BstPI 3899 GGTTACC G^GT[ACGT]ACC
DraIII 4163 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 5640 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3899 GGTTACC G^GT[ACGT]ACC
EcoRI 5802 GAATTC G^AATTC
EcoT22I 3268 ATGCAT ATGCA^T
FbaI 3921 TGATCA T^GATCA
FspI 405 TGCGCA TGC^GCA
HindIII 2275 AAGCTT A^AGCTT
KasI 905 GGCGCC G^GCGCC
KspI 3687 CCGCGG CCGC^GG
MfeI 2422 CAATTG C^AATTG
MunI 2422 CAATTG C^AATTG
NaeI 5182 GCCGGC GCC^GGC
NarI 905 GGCGCC GG^CGCC
NgoMIV 5182 GCCGGC G^CCGGC
NheI 2019 GCTAGC G^CTAGC
NsiI 3268 ATGCAT ATGCA^T
PacI 2581 TTAATTAA TTAAT^TAA
PaeR7I 1812 CTCGAG C^TCGAG
PflFI 1720 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 767 CACGTG CAC^GTG
PmlI 767 CACGTG CAC^GTG
PshAI 5101 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2616 AACGTT AA^CGTT
PvuI 3222 CGATCG CGAT^CG
SacII 3687 CCGCGG CCGC^GG
SapI 508 GCTCTTC GCTCTTC(1/4)
SfiI 3311 GGCCAGTCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 905 GGCGCC GGC^GCC
SnaBI 3414 TACGTA TAC^GTA
SpeI 2267 ACTAGT A^CTAGT
Tth111I 1720 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4087 TCTAGA T^CTAGA
XhoI 1812 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 46 enzymes) #
###################################
AatII 433 GACGTC GACGT^C
5707 GACGTC
Acc65I 2490 GGTACC G^GTACC
4191 GGTACC
AccII 3220 CGCG CG^CG
3688 CGCG
AflII 3110 CTTAAG C^TTAAG
3149 CTTAAG
AlwNI 3363 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
3654 CAGTACCTG
AocI 1201 CCTGAGG CC^T[ACGT]AGG
5283 CCTGAGG
Asp718I 2490 GGTACC G^GTACC
4191 GGTACC
BalI 559 TGGCCA TGG^CCA
1325 TGGCCA
BfrI 3110 CTTAAG C^TTAAG
3149 CTTAAG
BglI 2451 GCCACACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3312 GCCAGTCAGGC
BlpI 956 GCTGAGC GC^T[ACGT]AGC
1341 GCTCAGC
Bpu1102I 956 GCTGAGC GC^T[ACGT]AGC
1341 GCTCAGC
BsaAI 767 CACGTG [CT]AC^GT[GA]
3414 TACGTA
BsaBI 3916 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4647 GATCTGCATC
BsaI 2673 GAGACC GGTCTC(1/5)
3522 GAGACC
BsrFI 2487 GCCGGT [GA]^CCGG[CT]
5182 GCCGGC
BstAPI 539 GCATACTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3362 GCAGGCACTGC
BstUI 3220 CGCG CG^CG
3688 CGCG
BstXI 999 CCACTGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2421 CCAATTGTGTGG
Bsu36I 1201 CCTGAGG CC^T[ACGT]AGG
5283 CCTGAGG
CelII 956 GCTGAGC GC^T[ACGT]AGC
1341 GCTCAGC
Cfr10I 2487 GCCGGT [GA]^CCGG[CT]
5182 GCCGGC
DrdI 476 GACAAACACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3625 GACCTTGGAGTC
EagI 72 CGGCCG C^GGCCG
125 CGGCCG
EclXI 72 CGGCCG C^GGCCG
125 CGGCCG
Eco52I 72 CGGCCG C^GGCCG
125 CGGCCG
Eco81I 1201 CCTGAGG CC^T[ACGT]AGG
5283 CCTGAGG
HaeII 905 GGCGCC [GA]GCGC^[CT]
1317 AGCGCC
HpaI 608 GTTAAC GTT^AAC
4058 GTTAAC
KpnI 2490 GGTACC GGTAC^C
4191 GGTACC
MamI 3916 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4647 GATCTGCATC
MluNI 559 TGGCCA TGG^CCA
1325 TGGCCA
MscI 559 TGGCCA TGG^CCA
1325 TGGCCA
MslI 4049 CACAGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
5133 CATTTCCATG
MvnI 3220 CGCG CG^CG
3688 CGCG
NcoI 562 CCATGG C^CATGG
1898 CCATGG
PflMI 2680 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4892 CCAAATACTGG
PstI 185 CTGCAG CTGCA^G
379 CTGCAG
PvuII 98 CAGCTG CAG^CTG
2684 CAGCTG
SmaI 61 CCCGGG CCC^GGG
1710 CCCGGG
SspI 4227 AATATT AAT^ATT
4913 AATATT
StuI 413 AGGCCT AGG^CCT
5613 AGGCCT
Van91I 2680 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4892 CCAAATACTGG
XcmI 1970 CCAGACTTTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5190 CCAAGGGGCAGATGG
XmaCI 61 CCCGGG C^CCGGG
1710 CCCGGG
XmaI 61 CCCGGG C^CCGGG
1710 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AluI AlwI ApoI Asp700I
AspHI AvaI AvaII BanI BbsI
BbvCI BbvI BciVI BcnI BfaI
BglII BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspLU11I
BsrBI BsrDI BsrGI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI