################################## # NO CUT ENZYMES ( 50 enzymes) # ################################## AccIII AgeI AhdI Aor51HI AscI AseI AsnI AspEI AvrII BamHI BcgI BlnI BsaWI BseAI BsiWI BspDI BspEI BspHI BssHII Bst1107I BstBI BstZ17I ClaI CpoI Eam1105I Eco47III EcoRV FseI MluI MroI NdeI NotI NruI NspV PinAI PmeI PpuMI PshBI RcaI RsrII SacI SalI SbfI ScaI SexAI SfuI SgrAI SphI Sse8387I SwaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AclI 2616 AACGTT AA^CGTT Alw44I 1524 GTGCAC G^TGCAC ApaI 1412 GGGCCC GGGCC^C ApaLI 1524 GTGCAC G^TGCAC AspI 1720 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 405 TGCGCA TGC^GCA BanII 1412 GGGCCC G[GA]GC[CT]^C BbeI 905 GGCGCC GGCGC^C BbrPI 767 CACGTG CAC^GTG BclI 3921 TGATCA T^GATCA BspMI 5587 ACCTGC ACCTGC(4/8) BssSI 1155 CTCGTG CACGAG(-5/-1) BstEII 3899 GGTTACC G^GT[ACGT]ACC BstPI 3899 GGTTACC G^GT[ACGT]ACC DraIII 4163 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 5640 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 3899 GGTTACC G^GT[ACGT]ACC EcoRI 5802 GAATTC G^AATTC EcoT22I 3268 ATGCAT ATGCA^T FbaI 3921 TGATCA T^GATCA FspI 405 TGCGCA TGC^GCA HindIII 2275 AAGCTT A^AGCTT KasI 905 GGCGCC G^GCGCC KspI 3687 CCGCGG CCGC^GG MfeI 2422 CAATTG C^AATTG MunI 2422 CAATTG C^AATTG NaeI 5182 GCCGGC GCC^GGC NarI 905 GGCGCC GG^CGCC NgoMIV 5182 GCCGGC G^CCGGC NheI 2019 GCTAGC G^CTAGC NsiI 3268 ATGCAT ATGCA^T PacI 2581 TTAATTAA TTAAT^TAA PaeR7I 1812 CTCGAG C^TCGAG PflFI 1720 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 767 CACGTG CAC^GTG PmlI 767 CACGTG CAC^GTG PshAI 5101 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 2616 AACGTT AA^CGTT PvuI 3222 CGATCG CGAT^CG SacII 3687 CCGCGG CCGC^GG SapI 508 GCTCTTC GCTCTTC(1/4) SfiI 3311 GGCCAGTCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 905 GGCGCC GGC^GCC SnaBI 3414 TACGTA TAC^GTA SpeI 2267 ACTAGT A^CTAGT Tth111I 1720 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 4087 TCTAGA T^CTAGA XhoI 1812 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 46 enzymes) # ################################### AatII 433 GACGTC GACGT^C 5707 GACGTC Acc65I 2490 GGTACC G^GTACC 4191 GGTACC AccII 3220 CGCG CG^CG 3688 CGCG AflII 3110 CTTAAG C^TTAAG 3149 CTTAAG AlwNI 3363 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG 3654 CAGTACCTG AocI 1201 CCTGAGG CC^T[ACGT]AGG 5283 CCTGAGG Asp718I 2490 GGTACC G^GTACC 4191 GGTACC BalI 559 TGGCCA TGG^CCA 1325 TGGCCA BfrI 3110 CTTAAG C^TTAAG 3149 CTTAAG BglI 2451 GCCACACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3312 GCCAGTCAGGC BlpI 956 GCTGAGC GC^T[ACGT]AGC 1341 GCTCAGC Bpu1102I 956 GCTGAGC GC^T[ACGT]AGC 1341 GCTCAGC BsaAI 767 CACGTG [CT]AC^GT[GA] 3414 TACGTA BsaBI 3916 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4647 GATCTGCATC BsaI 2673 GAGACC GGTCTC(1/5) 3522 GAGACC BsrFI 2487 GCCGGT [GA]^CCGG[CT] 5182 GCCGGC BstAPI 539 GCATACTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3362 GCAGGCACTGC BstUI 3220 CGCG CG^CG 3688 CGCG BstXI 999 CCACTGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2421 CCAATTGTGTGG Bsu36I 1201 CCTGAGG CC^T[ACGT]AGG 5283 CCTGAGG CelII 956 GCTGAGC GC^T[ACGT]AGC 1341 GCTCAGC Cfr10I 2487 GCCGGT [GA]^CCGG[CT] 5182 GCCGGC DrdI 476 GACAAACACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3625 GACCTTGGAGTC EagI 72 CGGCCG C^GGCCG 125 CGGCCG EclXI 72 CGGCCG C^GGCCG 125 CGGCCG Eco52I 72 CGGCCG C^GGCCG 125 CGGCCG Eco81I 1201 CCTGAGG CC^T[ACGT]AGG 5283 CCTGAGG HaeII 905 GGCGCC [GA]GCGC^[CT] 1317 AGCGCC HpaI 608 GTTAAC GTT^AAC 4058 GTTAAC KpnI 2490 GGTACC GGTAC^C 4191 GGTACC MamI 3916 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4647 GATCTGCATC MluNI 559 TGGCCA TGG^CCA 1325 TGGCCA MscI 559 TGGCCA TGG^CCA 1325 TGGCCA MslI 4049 CACAGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 5133 CATTTCCATG MvnI 3220 CGCG CG^CG 3688 CGCG NcoI 562 CCATGG C^CATGG 1898 CCATGG PflMI 2680 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4892 CCAAATACTGG PstI 185 CTGCAG CTGCA^G 379 CTGCAG PvuII 98 CAGCTG CAG^CTG 2684 CAGCTG SmaI 61 CCCGGG CCC^GGG 1710 CCCGGG SspI 4227 AATATT AAT^ATT 4913 AATATT StuI 413 AGGCCT AGG^CCT 5613 AGGCCT Van91I 2680 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4892 CCAAATACTGG XcmI 1970 CCAGACTTTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 5190 CCAAGGGGCAGATGG XmaCI 61 CCCGGG C^CCGGG 1710 CCCGGG XmaI 61 CCCGGG C^CCGGG 1710 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AccI AciI AcsI AcyI AfaI AflIII AluI AlwI ApoI Asp700I AspHI AvaI AvaII BanI BbsI BbvCI BbvI BciVI BcnI BfaI BglII BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspLU11I BsrBI BsrDI BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI