################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## AatII Acc65I AccIII AclI AgeI AhdI AscI Asp718I AspEI AviII AvrII BamHI BbeI BcgI BlnI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BsrGI BstBI BstEII BstPI ClaI CpoI DraIII EagI Eam1105I EclXI Eco52I EcoO65I EcoT22I FseI FspI KasI KpnI KspI MfeI MluI MroI MunI NarI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI XhoI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AcyI 547 GACGCC G[GA]^CG[CT]C Alw44I 5546 GTGCAC G^TGCAC Aor51HI 830 AGCGCT AGC^GCT ApaLI 5546 GTGCAC G^TGCAC AseI 2479 ATTAAT AT^TAAT AsnI 2479 ATTAAT AT^TAAT BalI 1322 TGGCCA TGG^CCA BbrPI 268 CACGTG CAC^GTG BclI 4641 TGATCA T^GATCA BsaAI 268 CACGTG [CT]AC^GT[GA] BsaHI 547 GACGCC G[GA]^CG[CT]C BsaWI 898 ACCGGA [AT]^CCGG[AT] BsmBI 1258 GAGACG CGTCTC(1/5) BsmI 3204 GAATGC GAATGC(1/-1) BspLU11I 3653 ACATGT A^CATGT BsrBI 1744 GAGCGG CCGCTC(-3/-3) BsrDI 3304 CATTGC GCAATG(2/0) BssHII 576 GCGCGC G^CGCGC Bst1107I 1966 GTATAC GTA^TAC BstAPI 3784 GCATGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstZ17I 1966 GTATAC GTA^TAC Eco47III 830 AGCGCT AGC^GCT EcoRI 5318 GAATTC G^AATTC EcoRV 2323 GATATC GAT^ATC FbaI 4641 TGATCA T^GATCA Hin1I 547 GACGCC G[GA]^CG[CT]C HindIII 2664 AAGCTT A^AGCTT HpaI 4725 GTTAAC GTT^AAC MluNI 1322 TGGCCA TGG^CCA MscI 1322 TGGCCA TGG^CCA NcoI 1104 CCATGG C^CATGG PflMI 206 CCAACATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 268 CACGTG CAC^GTG PmlI 268 CACGTG CAC^GTG PshBI 2479 ATTAAT AT^TAAT SexAI 515 ACCTGGT A^CC[AT]GGT SmaI 1814 CCCGGG CCC^GGG SspI 2960 AATATT AAT^ATT SwaI 4335 ATTTAAAT ATTT^AAAT Van91I 206 CCAACATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 1814 CCCGGG C^CCGGG XmaI 1814 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccI 1966 GTATAC GT^[AC][GT]AC 3400 GTAGAC AflII 2412 CTTAAG C^TTAAG 5048 CTTAAG AflIII 1421 ACGTGT A^C[GA][CT]GT 3653 ACATGT Asp700I 3492 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3514 GAATCATTTC AspI 1192 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2001 GACACAGTC AvaI 1814 CCCGGG C^[CT]CG[GA]G 3578 CTCGGG BbvCI 1558 CCTCAGC CCTCAGC(-5/-2) 2489 GCTGAGG BciVI 4355 GGATAC GTATCC(6/5) 964 GTATCC BfrI 2412 CTTAAG C^TTAAG 5048 CTTAAG BglI 2528 GCCAGCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3886 GCCTTGAAGGC BglII 1847 AGATCT A^GATCT 5475 AGATCT BsgI 3755 CTGCAC GTGCAG(16/14) 4436 GTGCAG BsoBI 1814 CCCGGG C^[CT]CG[GA]G 3578 CTCGGG BssSI 2603 CACGAG CACGAG(-5/-1) 3180 CTCGTG DrdI 232 GACAAGATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1147 GACATCTCGGTC EcoNI 954 CCTCCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1041 CCTCATGGAGG FauI 867 CCCGC CCCGC(4/6) 2440 CCCGC HgaI 547 GACGC GACGC(5/10) 1051 GACGC NaeI 580 GCCGGC GCC^GGC 3977 GCCGGC NdeI 3670 CATATG CA^TATG 4967 CATATG NgoMIV 580 GCCGGC G^CCGGC 3977 GCCGGC NheI 2537 GCTAGC G^CTAGC 2778 GCTAGC PflFI 1192 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2001 GACACAGTC PshAI 4942 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 5517 GACTGGAGTC SapI 1593 GAAGAGC GCTCTTC(1/4) 2544 GAAGAGC StuI 159 AGGCCT AGG^CCT 2830 AGGCCT Tth111I 1192 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2001 GACACAGTC XbaI 1455 TCTAGA T^CTAGA 4424 TCTAGA XmnI 3492 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3514 GAATCATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 118 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI AocI ApaI ApoI AspHI AvaII BanI BanII BbsI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaBI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII