##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI AscI Asp718I AspEI AviII
AvrII BamHI BbeI BcgI BlnI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BsrGI BstBI BstEII BstPI
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco52I EcoO65I EcoT22I FseI
FspI KasI KpnI KspI MfeI
MluI MroI MunI NarI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AcyI 547 GACGCC G[GA]^CG[CT]C
Alw44I 5546 GTGCAC G^TGCAC
Aor51HI 830 AGCGCT AGC^GCT
ApaLI 5546 GTGCAC G^TGCAC
AseI 2479 ATTAAT AT^TAAT
AsnI 2479 ATTAAT AT^TAAT
BalI 1322 TGGCCA TGG^CCA
BbrPI 268 CACGTG CAC^GTG
BclI 4641 TGATCA T^GATCA
BsaAI 268 CACGTG [CT]AC^GT[GA]
BsaHI 547 GACGCC G[GA]^CG[CT]C
BsaWI 898 ACCGGA [AT]^CCGG[AT]
BsmBI 1258 GAGACG CGTCTC(1/5)
BsmI 3204 GAATGC GAATGC(1/-1)
BspLU11I 3653 ACATGT A^CATGT
BsrBI 1744 GAGCGG CCGCTC(-3/-3)
BsrDI 3304 CATTGC GCAATG(2/0)
BssHII 576 GCGCGC G^CGCGC
Bst1107I 1966 GTATAC GTA^TAC
BstAPI 3784 GCATGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 1966 GTATAC GTA^TAC
Eco47III 830 AGCGCT AGC^GCT
EcoRI 5318 GAATTC G^AATTC
EcoRV 2323 GATATC GAT^ATC
FbaI 4641 TGATCA T^GATCA
Hin1I 547 GACGCC G[GA]^CG[CT]C
HindIII 2664 AAGCTT A^AGCTT
HpaI 4725 GTTAAC GTT^AAC
MluNI 1322 TGGCCA TGG^CCA
MscI 1322 TGGCCA TGG^CCA
NcoI 1104 CCATGG C^CATGG
PflMI 206 CCAACATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 268 CACGTG CAC^GTG
PmlI 268 CACGTG CAC^GTG
PshBI 2479 ATTAAT AT^TAAT
SexAI 515 ACCTGGT A^CC[AT]GGT
SmaI 1814 CCCGGG CCC^GGG
SspI 2960 AATATT AAT^ATT
SwaI 4335 ATTTAAAT ATTT^AAAT
Van91I 206 CCAACATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1814 CCCGGG C^CCGGG
XmaI 1814 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 1966 GTATAC GT^[AC][GT]AC
3400 GTAGAC
AflII 2412 CTTAAG C^TTAAG
5048 CTTAAG
AflIII 1421 ACGTGT A^C[GA][CT]GT
3653 ACATGT
Asp700I 3492 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3514 GAATCATTTC
AspI 1192 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2001 GACACAGTC
AvaI 1814 CCCGGG C^[CT]CG[GA]G
3578 CTCGGG
BbvCI 1558 CCTCAGC CCTCAGC(-5/-2)
2489 GCTGAGG
BciVI 4355 GGATAC GTATCC(6/5)
964 GTATCC
BfrI 2412 CTTAAG C^TTAAG
5048 CTTAAG
BglI 2528 GCCAGCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3886 GCCTTGAAGGC
BglII 1847 AGATCT A^GATCT
5475 AGATCT
BsgI 3755 CTGCAC GTGCAG(16/14)
4436 GTGCAG
BsoBI 1814 CCCGGG C^[CT]CG[GA]G
3578 CTCGGG
BssSI 2603 CACGAG CACGAG(-5/-1)
3180 CTCGTG
DrdI 232 GACAAGATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1147 GACATCTCGGTC
EcoNI 954 CCTCCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1041 CCTCATGGAGG
FauI 867 CCCGC CCCGC(4/6)
2440 CCCGC
HgaI 547 GACGC GACGC(5/10)
1051 GACGC
NaeI 580 GCCGGC GCC^GGC
3977 GCCGGC
NdeI 3670 CATATG CA^TATG
4967 CATATG
NgoMIV 580 GCCGGC G^CCGGC
3977 GCCGGC
NheI 2537 GCTAGC G^CTAGC
2778 GCTAGC
PflFI 1192 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2001 GACACAGTC
PshAI 4942 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5517 GACTGGAGTC
SapI 1593 GAAGAGC GCTCTTC(1/4)
2544 GAAGAGC
StuI 159 AGGCCT AGG^CCT
2830 AGGCCT
Tth111I 1192 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2001 GACACAGTC
XbaI 1455 TCTAGA T^CTAGA
4424 TCTAGA
XmnI 3492 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3514 GAATCATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AvaII BanI BanII BbsI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaBI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RcaI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII