##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AccIII AclI AcyI AhdI
ApaI AscI AseI AsnI Asp700I
AspEI AviII BbeI BbvCI BcgI
BglI BsaHI BseAI BspDI BspEI
BssHII Bst1107I BstAPI BstBI BstZ17I
ClaI CpoI EagI Eam1105I EclXI
Eco52I EcoRV FseI FspI Hin1I
KasI KspI MroI NarI NdeI
NheI NotI NruI NspV PacI
PaeR7I PmeI PshAI PshBI Psp1406I
PvuI RsrII SacII SfiI SfoI
SfuI SmaI SnaBI SphI SspI
SwaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 27 enzymes) #
###################################
AgeI 4094 ACCGGT A^CCGGT
BamHI 1274 GGATCC G^GATCC
BbsI 2301 GAAGAC GAAGAC(2/6)
BpuAI 2301 GAAGAC GAAGAC(2/6)
BsaBI 71 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 5289 CGGTCG CG[GA][CT]^CG
BsiWI 2455 CGTACG C^GTACG
BsmBI 1209 GAGACG CGTCTC(1/5)
BsrBI 1967 CCGCTC CCGCTC(-3/-3)
BsrDI 2126 CATTGC GCAATG(2/0)
EcoT22I 2292 ATGCAT ATGCA^T
HpaI 464 GTTAAC GTT^AAC
MamI 71 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4869 CAATTG C^AATTG
MluI 2364 ACGCGT A^CGCGT
MunI 4869 CAATTG C^AATTG
NaeI 3268 GCCGGC GCC^GGC
NgoMIV 3268 GCCGGC G^CCGGC
NsiI 2292 ATGCAT ATGCA^T
PinAI 4094 ACCGGT A^CCGGT
PpuMI 1676 AGGACCT [GA]G^G[AT]CC[CT]
SalI 2664 GTCGAC G^TCGAC
SbfI 3071 CCTGCAGG CCTGCA^GG
ScaI 2090 AGTACT AGT^ACT
SgrAI 4093 CACCGGTG C[GA]^CCGG[CT]G
SpeI 5183 ACTAGT A^CTAGT
Sse8387I 3071 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 47 enzymes) #
###################################
AccII 2365 CGCG CG^CG
4559 CGCG
AflII 3908 CTTAAG C^TTAAG
5154 CTTAAG
Alw44I 2079 GTGCAC G^TGCAC
2370 GTGCAC
AocI 1494 CCTCAGG CC^T[ACGT]AGG
4169 CCTGAGG
Aor51HI 385 AGCGCT AGC^GCT
4735 AGCGCT
ApaLI 2079 GTGCAC G^TGCAC
2370 GTGCAC
AspI 2667 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3127 GACGTAGTC
AvrII 4609 CCTAGG C^CTAGG
4686 CCTAGG
BalI 565 TGGCCA TGG^CCA
2158 TGGCCA
BbrPI 2373 CACGTG CAC^GTG
4391 CACGTG
BciVI 1066 GGATAC GTATCC(6/5)
5787 GGATAC
BclI 3394 TGATCA T^GATCA
3508 TGATCA
BfrI 3908 CTTAAG C^TTAAG
5154 CTTAAG
BlnI 4609 CCTAGG C^CTAGG
4686 CCTAGG
BsaWI 632 TCCGGT [AT]^CCGG[AT]
4094 ACCGGT
Bsp1407I 4972 TGTACA T^GTACA
5220 TGTACA
BspHI 4286 TCATGA T^CATGA
5025 TCATGA
BsrFI 3268 GCCGGC [GA]^CCGG[CT]
4094 ACCGGT
BsrGI 4972 TGTACA T^GTACA
5220 TGTACA
BssSI 2162 CACGAG CACGAG(-5/-1)
4214 CTCGTG
BstEII 606 GGTGACC G^GT[ACGT]ACC
2795 GGTAACC
BstPI 606 GGTGACC G^GT[ACGT]ACC
2795 GGTAACC
BstUI 2365 CGCG CG^CG
4559 CGCG
Bsu36I 1494 CCTCAGG CC^T[ACGT]AGG
4169 CCTGAGG
Cfr10I 3268 GCCGGC [GA]^CCGG[CT]
4094 ACCGGT
DraII 1676 AGGACCT [GA]G^G[ACGT]CC[CT]
3903 AGGCCCT
DraIII 2073 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
5749 CACCTGGTG
DrdI 1889 GACTGAAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3698 GACATGTCTGTC
Eco47III 385 AGCGCT AGC^GCT
4735 AGCGCT
Eco81I 1494 CCTCAGG CC^T[ACGT]AGG
4169 CCTGAGG
EcoO109I 1676 AGGACCT [GA]G^G[ACGT]CC[CT]
3903 AGGCCCT
EcoO65I 606 GGTGACC G^GT[ACGT]ACC
2795 GGTAACC
EcoRI 282 GAATTC G^AATTC
1013 GAATTC
FbaI 3394 TGATCA T^GATCA
3508 TGATCA
HindIII 2262 AAGCTT A^AGCTT
2615 AAGCTT
MluNI 565 TGGCCA TGG^CCA
2158 TGGCCA
MscI 565 TGGCCA TGG^CCA
2158 TGGCCA
MvnI 2365 CGCG CG^CG
4559 CGCG
NcoI 1388 CCATGG C^CATGG
4024 CCATGG
PflFI 2667 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3127 GACGTAGTC
PmaCI 2373 CACGTG CAC^GTG
4391 CACGTG
PmlI 2373 CACGTG CAC^GTG
4391 CACGTG
RcaI 4286 TCATGA T^CATGA
5025 TCATGA
SspBI 4972 TGTACA T^GTACA
5220 TGTACA
StuI 1372 AGGCCT AGG^CCT
1538 AGGCCT
Tth111I 2667 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3127 GACGTAGTC
XbaI 2806 TCTAGA T^CTAGA
3447 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
Acc65I AccI AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp718I AspHI AvaI AvaII BanI
BanII BbvI BcnI BfaI BglII
BlpI BmyI BpmI Bpu1102I BsaAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspLU11I BspMI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DsaI EaeI EarI
EcoNI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI KpnI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII