################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## AatII AccIII AclI AcyI AhdI ApaI AscI AseI AsnI Asp700I AspEI AviII BbeI BbvCI BcgI BglI BsaHI BseAI BspDI BspEI BssHII Bst1107I BstAPI BstBI BstZ17I ClaI CpoI EagI Eam1105I EclXI Eco52I EcoRV FseI FspI Hin1I KasI KspI MroI NarI NdeI NheI NotI NruI NspV PacI PaeR7I PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SfiI SfoI SfuI SmaI SnaBI SphI SspI SwaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 27 enzymes) # ################################### AgeI 4094 ACCGGT A^CCGGT BamHI 1274 GGATCC G^GATCC BbsI 2301 GAAGAC GAAGAC(2/6) BpuAI 2301 GAAGAC GAAGAC(2/6) BsaBI 71 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 5289 CGGTCG CG[GA][CT]^CG BsiWI 2455 CGTACG C^GTACG BsmBI 1209 GAGACG CGTCTC(1/5) BsrBI 1967 CCGCTC CCGCTC(-3/-3) BsrDI 2126 CATTGC GCAATG(2/0) EcoT22I 2292 ATGCAT ATGCA^T HpaI 464 GTTAAC GTT^AAC MamI 71 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 4869 CAATTG C^AATTG MluI 2364 ACGCGT A^CGCGT MunI 4869 CAATTG C^AATTG NaeI 3268 GCCGGC GCC^GGC NgoMIV 3268 GCCGGC G^CCGGC NsiI 2292 ATGCAT ATGCA^T PinAI 4094 ACCGGT A^CCGGT PpuMI 1676 AGGACCT [GA]G^G[AT]CC[CT] SalI 2664 GTCGAC G^TCGAC SbfI 3071 CCTGCAGG CCTGCA^GG ScaI 2090 AGTACT AGT^ACT SgrAI 4093 CACCGGTG C[GA]^CCGG[CT]G SpeI 5183 ACTAGT A^CTAGT Sse8387I 3071 CCTGCAGG CCTGCA^GG ################################### # TWO CUT ENZYMES ( 47 enzymes) # ################################### AccII 2365 CGCG CG^CG 4559 CGCG AflII 3908 CTTAAG C^TTAAG 5154 CTTAAG Alw44I 2079 GTGCAC G^TGCAC 2370 GTGCAC AocI 1494 CCTCAGG CC^T[ACGT]AGG 4169 CCTGAGG Aor51HI 385 AGCGCT AGC^GCT 4735 AGCGCT ApaLI 2079 GTGCAC G^TGCAC 2370 GTGCAC AspI 2667 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3127 GACGTAGTC AvrII 4609 CCTAGG C^CTAGG 4686 CCTAGG BalI 565 TGGCCA TGG^CCA 2158 TGGCCA BbrPI 2373 CACGTG CAC^GTG 4391 CACGTG BciVI 1066 GGATAC GTATCC(6/5) 5787 GGATAC BclI 3394 TGATCA T^GATCA 3508 TGATCA BfrI 3908 CTTAAG C^TTAAG 5154 CTTAAG BlnI 4609 CCTAGG C^CTAGG 4686 CCTAGG BsaWI 632 TCCGGT [AT]^CCGG[AT] 4094 ACCGGT Bsp1407I 4972 TGTACA T^GTACA 5220 TGTACA BspHI 4286 TCATGA T^CATGA 5025 TCATGA BsrFI 3268 GCCGGC [GA]^CCGG[CT] 4094 ACCGGT BsrGI 4972 TGTACA T^GTACA 5220 TGTACA BssSI 2162 CACGAG CACGAG(-5/-1) 4214 CTCGTG BstEII 606 GGTGACC G^GT[ACGT]ACC 2795 GGTAACC BstPI 606 GGTGACC G^GT[ACGT]ACC 2795 GGTAACC BstUI 2365 CGCG CG^CG 4559 CGCG Bsu36I 1494 CCTCAGG CC^T[ACGT]AGG 4169 CCTGAGG Cfr10I 3268 GCCGGC [GA]^CCGG[CT] 4094 ACCGGT DraII 1676 AGGACCT [GA]G^G[ACGT]CC[CT] 3903 AGGCCCT DraIII 2073 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG 5749 CACCTGGTG DrdI 1889 GACTGAAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3698 GACATGTCTGTC Eco47III 385 AGCGCT AGC^GCT 4735 AGCGCT Eco81I 1494 CCTCAGG CC^T[ACGT]AGG 4169 CCTGAGG EcoO109I 1676 AGGACCT [GA]G^G[ACGT]CC[CT] 3903 AGGCCCT EcoO65I 606 GGTGACC G^GT[ACGT]ACC 2795 GGTAACC EcoRI 282 GAATTC G^AATTC 1013 GAATTC FbaI 3394 TGATCA T^GATCA 3508 TGATCA HindIII 2262 AAGCTT A^AGCTT 2615 AAGCTT MluNI 565 TGGCCA TGG^CCA 2158 TGGCCA MscI 565 TGGCCA TGG^CCA 2158 TGGCCA MvnI 2365 CGCG CG^CG 4559 CGCG NcoI 1388 CCATGG C^CATGG 4024 CCATGG PflFI 2667 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3127 GACGTAGTC PmaCI 2373 CACGTG CAC^GTG 4391 CACGTG PmlI 2373 CACGTG CAC^GTG 4391 CACGTG RcaI 4286 TCATGA T^CATGA 5025 TCATGA SspBI 4972 TGTACA T^GTACA 5220 TGTACA StuI 1372 AGGCCT AGG^CCT 1538 AGGCCT Tth111I 2667 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3127 GACGTAGTC XbaI 2806 TCTAGA T^CTAGA 3447 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 119 enzymes) # ######################################### Acc65I AccI AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI Asp718I AspHI AvaI AvaII BanI BanII BbvI BcnI BfaI BglII BlpI BmyI BpmI Bpu1102I BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspLU11I BspMI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DsaI EaeI EarI EcoNI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII