##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
Acc65I AccIII AgeI AhdI Alw44I
ApaLI AscI Asp718I AspEI AviII
AvrII BalI BlnI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BsrBI
BssHII ClaI CpoI EagI Eam1105I
EclXI Eco52I EcoRI FseI FspI
KpnI KspI MluI MluNI MroI
MscI NheI NotI NruI PacI
PaeR7I PinAI PmeI PshAI PvuI
RsrII SacII SalI SbfI SexAI
SfiI SgrAI SnaBI SpeI Sse8387I
SspI SwaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 3071 GACGTC GACGT^C
AclI 2365 AACGTT AA^CGTT
AflII 896 CTTAAG C^TTAAG
AseI 5856 ATTAAT AT^TAAT
AsnI 5856 ATTAAT AT^TAAT
BbeI 2039 GGCGCC GGCGC^C
BbrPI 5559 CACGTG CAC^GTG
BcgI 1169 CGAACAAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3633 TGATCA T^GATCA
BfrI 896 CTTAAG C^TTAAG
BglI 4174 GCCAACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 2498 GATTTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1305 TCCGGT [AT]^CCGG[AT]
BspHI 4396 TCATGA T^CATGA
BsrDI 345 CATTGC GCAATG(2/0)
DsaI 2172 CCATGG C^C[GA][CT]GG
FbaI 3633 TGATCA T^GATCA
HpaI 4145 GTTAAC GTT^AAC
KasI 2039 GGCGCC G^GCGCC
MamI 2498 GATTTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1788 CAATTG C^AATTG
MunI 1788 CAATTG C^AATTG
NaeI 1736 GCCGGC GCC^GGC
NarI 2039 GGCGCC GG^CGCC
NcoI 2172 CCATGG C^CATGG
NgoMIV 1736 GCCGGC G^CCGGC
PmaCI 5559 CACGTG CAC^GTG
PmlI 5559 CACGTG CAC^GTG
PshBI 5856 ATTAAT AT^TAAT
Psp1406I 2365 AACGTT AA^CGTT
RcaI 4396 TCATGA T^CATGA
SfoI 2039 GGCGCC GGC^GCC
SmaI 3473 CCCGGG CCC^GGG
StuI 4181 AGGCCT AGG^CCT
XmaCI 3473 CCCGGG C^CCGGG
XmaI 3473 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
AccII 1398 CGCG CG^CG
4570 CGCG
AcyI 2039 GGCGCC G[GA]^CG[CT]C
3071 GACGTC
AocI 3749 CCTCAGG CC^T[ACGT]AGG
6551 CCTCAGG
Aor51HI 1513 AGCGCT AGC^GCT
3086 AGCGCT
ApaI 1362 GGGCCC GGGCC^C
2698 GGGCCC
Asp700I 1423 GAATAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1666 GAAGCAGTTC
BsaAI 5079 CACGTA [CT]AC^GT[GA]
5559 CACGTG
BsaHI 2039 GGCGCC G[GA]^CG[CT]C
3071 GACGTC
Bsp1407I 5227 TGTACA T^GTACA
6848 TGTACA
BsrFI 1736 GCCGGC [GA]^CCGG[CT]
5373 ACCGGC
BsrGI 5227 TGTACA T^GTACA
6848 TGTACA
Bst1107I 5269 GTATAC GTA^TAC
6838 GTATAC
BstAPI 422 GCATGGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3374 GCACGTCCTGC
BstBI 51 TTCGAA TT^CGAA
1167 TTCGAA
BstEII 1282 GGTGACC G^GT[ACGT]ACC
4495 GGTAACC
BstPI 1282 GGTGACC G^GT[ACGT]ACC
4495 GGTAACC
BstUI 1398 CGCG CG^CG
4570 CGCG
BstZ17I 5269 GTATAC GTA^TAC
6838 GTATAC
Bsu36I 3749 CCTCAGG CC^T[ACGT]AGG
6551 CCTCAGG
Cfr10I 1736 GCCGGC [GA]^CCGG[CT]
5373 ACCGGC
DraIII 1016 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
4035 CACTTAGTG
EaeI 5365 TGGCCG [CT]^GGCC[GA]
5537 TGGCCG
Eco47III 1513 AGCGCT AGC^GCT
3086 AGCGCT
Eco81I 3749 CCTCAGG CC^T[ACGT]AGG
6551 CCTCAGG
EcoNI 873 CCTTTGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4523 CCTACAACAGG
EcoO65I 1282 GGTGACC G^GT[ACGT]ACC
4495 GGTAACC
EcoRV 1010 GATATC GAT^ATC
5956 GATATC
EcoT14I 1219 CCTTGG C^C[AT][AT]GG
2172 CCATGG
EcoT22I 204 ATGCAT ATGCA^T
280 ATGCAT
Hin1I 2039 GGCGCC G[GA]^CG[CT]C
3071 GACGTC
HincII 3287 GTTGAC GT[CT]^[GA]AC
4145 GTTAAC
HindII 3287 GTTGAC GT[CT]^[GA]AC
4145 GTTAAC
HindIII 630 AAGCTT A^AGCTT
3907 AAGCTT
MvnI 1398 CGCG CG^CG
4570 CGCG
NsiI 204 ATGCAT ATGCA^T
280 ATGCAT
NspV 51 TTCGAA TT^CGAA
1167 TTCGAA
SacI 2261 GAGCTC GAGCT^C
5679 GAGCTC
SfuI 51 TTCGAA TT^CGAA
1167 TTCGAA
SphI 474 GCATGC GCATG^C
4170 GCATGC
SspBI 5227 TGTACA T^GTACA
6848 TGTACA
StyI 1219 CCTTGG C^C[AT][AT]GG
2172 CCATGG
XmnI 1423 GAATAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1666 GAAGCAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AspI AvaI AvaII BamHI BanI
BanII BbsI BbvCI BbvI BciVI
BcnI BfaI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspLU11I BspMI BsrI BssKI BssSI
BstF5I BstNI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DrdI EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeI
NdeII NlaIII NlaIV NspI PflFI
PflMI PleI PpuMI PstI PvuII
RsaI SapI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XbaI
XhoII